Biosensors for Extracellular Vesicles

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (10 August 2021) | Viewed by 4696

Special Issue Editor


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Guest Editor
Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain
Interests: extracellular vesicles; biosensors and bioanalytics; nanomaterial sensors
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Special Issue Information

Dear Colleagues,

I am pleased to invite you to contribute to this Special Issue on Biosensors for Extracellular Vesicles (EVs).

These vesicles play a key role in intercellular communication, since they contain lipids, proteins, and nucleic acids which can be transferred to the recipient cells. They are receiving great attention in recent years as potential biomarkers and therapeutic vectors.

EVs that are secreted into biological fluids can act as biomarkers for prognosis and monitoring the response to treatment in a range of diseases. This includes autoimmune, vascular diseases, and cancer, among others. Analysis of EVs from body fluids may serve as a source of biomarkers for liquid biopsy. However, the bottleneck at EV research that is limiting clinical translation is the current isolation and quantification techniques. Although various methods exist to analyze EVs, quantification and characterization of EVs in clinical samples remains challenging.

This Special Issue plans to gather current research and review papers on “Biosensors for Extracellular Vesicles”. It is expected that these devices will have a great impact on the development of novel point-of-care methods for diagnostics.

Prof. Dr. María Carmen Blanco-López
Guest Editor

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Published Papers (1 paper)

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Research

12 pages, 2255 KiB  
Article
Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip
by Gijung Kim, Min Chul Park, Seonae Jang, Daeyoung Han, Hojun Kim, Wonjune Kim, Honggu Chun and Sunghoon Kim
Biosensors 2021, 11(9), 347; https://doi.org/10.3390/bios11090347 - 19 Sep 2021
Cited by 5 | Viewed by 3962
Abstract
Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane [...] Read more.
Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane with 200 nm pores, positioned between two chambers, functions as the size-selective filter. Using the chip, EVs from cell culture media and human serum were separated. The separated EVs were analyzed by nanoparticle tracking analysis (NTA), scanning electron microscopy, and immunoblotting. The experimental results proved the selective separation of EVs in cell culture media and human serum. Moreover, the diffusion-based separation showed a high yield of EVs in human serum compared to ultracentrifuge-based separation. The EV recovery rate analyzed from NTA data was 42% for cell culture media samples. We expect the developed method to be a potential tool for EV separation for diagnosis and therapy because it does not require complicated processes such as immune, chemical reaction, and external force and is scalable by increasing the nanoporous membrane size. Full article
(This article belongs to the Special Issue Biosensors for Extracellular Vesicles)
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