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Cells
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State of the Art in Stem Cell Culture for Regenerative...
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State of the Art in Stem Cell Culture for Regenerative Medicine

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: 30 November 2024 | Viewed by 4049

Special Issue Editors

Dr. Franca Di Meglio

E-Mail Website
Guest Editor
Department of Public Health, University of Naples Federico II, 80131 Naples, Italy
Interests: cell differentiation; cardiac cell therapy; biomaterials; three-dimensional cell cultures; stem cells; extracellular matrix
Dr. Daria Nurzynska

E-Mail Website
Guest Editor
Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84084 Fisciano, Italy
Interests: anatomy; histology; cell biology; congenital heart defects; adult stem cells
Prof. Dr. Clotilde Castaldo

E-Mail Website
Guest Editor
Department of Public Health, University of Naples Federico II, 80131 Naples, Italy
Interests: cardiac regenerative medicine; decellularized tissues; extracellular matrix; biological scaffolds; iPSCs; cardiac progenitor cells

Special Issue Information

Dear Colleagues,

The unique properties of stem cells, namely self-renewal and differentiation, make them capable of tissue and organ maintenance, endowing abilities of regeneration during physiological cell turnover or after pathological cell injury and loss. For these reasons, stem cells of various differentiation potential, i.e., pluripotent, multipotent, and unipotent (tissue-specific), have been isolated, manipulated, and evaluated in the pre-clinical and clinical studies for their use in regenerative medicine. Since the in vitro properties of these cells do not necessarily translate into tissue or organ regeneration when administered in vivo, the interactions between cells, extracellular matrix/scaffold and cytokines/growth factors are considered essential for guaranteeing cell properties and overseeing regulation in the living tissue, giving rise to bioengineering. Nowadays, regenerative medicine investigates several in vitro cell enhancement strategies, aiming at long-term cell survival, differentiation and tissue-specific function without genomic instability and rejection. The scope of this Special Issue is to present and discuss (in the form of original research papers and descriptive reviews) the state of the art in cell culture for regenerative medicine.

Potential topics include, but are not limited to: embryonic and induced pluripotent stem cells or tissue-specific, unipotent stem cells; classic or three-dimensional support; decellularized native extracellular matrix or biomimetic bio-engineered scaffolds; growth factor stimulation or mechanical activation for differentiation and structural organization; and any other emerging approach influencing the in vitro translation of the in vivo conditions for stem cell culture.

Dr. Franca Di Meglio
Dr. Daria Nurzynska
Prof. Dr. Clotilde Castaldo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stem cells
  • cell culture techniques
  • regenerative medicine
  • tissue engineering

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Published Papers (2 papers)

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Research

14 pages, 6643 KiB  
Article
Transcription Factor-Mediated Generation of Dopaminergic Neurons from Human iPSCs—A Comparison of Methods
by Kirstin O. McDonald, Nikita M. A. Lyons, Luca K. C. Gray, Janet B. Xu, Lucia Schoderboeck, Stephanie M. Hughes and Indranil Basak
Cells 2024, 13(12), 1016; https://doi.org/10.3390/cells13121016 - 11 Jun 2024
Viewed by 2153
Abstract
Dopaminergic neurons are the predominant brain cells affected in Parkinson’s disease. With the limited availability of live human brain dopaminergic neurons to study pathological mechanisms of Parkinson’s disease, dopaminergic neurons have been generated from human-skin-cell-derived induced pluripotent stem cells. Originally, induced pluripotent stem-cell-derived [...] Read more.
Dopaminergic neurons are the predominant brain cells affected in Parkinson’s disease. With the limited availability of live human brain dopaminergic neurons to study pathological mechanisms of Parkinson’s disease, dopaminergic neurons have been generated from human-skin-cell-derived induced pluripotent stem cells. Originally, induced pluripotent stem-cell-derived dopaminergic neurons were generated using small molecules. These neurons took more than two months to mature. However, the transcription-factor-mediated differentiation of induced pluripotent stem cells has revealed quicker and cheaper methods to generate dopaminergic neurons. In this study, we compared and contrasted three protocols to generate induced pluripotent stem-cell-derived dopaminergic neurons using transcription-factor-mediated directed differentiation. We deviated from the established protocols using lentivirus transduction to stably integrate different transcription factors into the AAVS1 safe harbour locus of induced pluripotent stem cells. We used different media compositions to generate more than 90% of neurons in the culture, out of which more than 85% of the neurons were dopaminergic neurons within three weeks. Therefore, from our comparative study, we reveal that a combination of transcription factors along with small molecule treatment may be required to generate a pure population of human dopaminergic neurons. Full article
(This article belongs to the Special Issue State of the Art in Stem Cell Culture for Regenerative Medicine)
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15 pages, 5306 KiB  
Article
Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells
by Taeko Ichise, Hirotake Ichise and Yusuke Shimizu
Cells 2024, 13(7), 582; https://doi.org/10.3390/cells13070582 - 27 Mar 2024
Viewed by 1217
Abstract
Human adipose-derived stromal cells (ADSCs) are an important resource for cell-based therapies. However, the dynamics of ADSCs after transplantation and their mechanisms of action in recipients remain unclear. Herein, we generated genetically engineered mouse ADSCs to clarify their biodistribution and post-transplantation status and [...] Read more.
Human adipose-derived stromal cells (ADSCs) are an important resource for cell-based therapies. However, the dynamics of ADSCs after transplantation and their mechanisms of action in recipients remain unclear. Herein, we generated genetically engineered mouse ADSCs to clarify their biodistribution and post-transplantation status and to analyze their role in recipient mesenchymal tissue modeling. Immortalized ADSCs (iADSCs) retained ADSC characteristics such as stromal marker gene expression and differentiation potential. iADSCs expressing a fluorescent reporter gene were seeded into biocompatible nonwoven fabric sheets and transplanted into the dorsal subcutaneous region of neonatal mice. Transplanted donor ADSCs were distributed as CD90-positive stromal cells on the sheets and survived 1 month after transplantation. Although accumulation of T lymphocytes or macrophages inside the sheet was not observed with or without donor cells, earlier migration and accumulation of recipient blood vascular endothelial cells (ECs) inside the sheet was observed in the presence of donor cells. Thus, our mouse model can help in studying the interplay between donor ADSCs and recipient cells over a 1-month period. This system may be of value for assessing and screening bioengineered ADSCs in vivo for optimal cell-based therapies. Full article
(This article belongs to the Special Issue State of the Art in Stem Cell Culture for Regenerative Medicine)
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