Cellular and Molecular Mechanisms in Bone and Cartilage Damage and Regeneration
A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Cellular Pathology".
Deadline for manuscript submissions: 28 February 2025 | Viewed by 1253
Special Issue Editor
Interests: cell therapy; bone union; cartilage regeneration; osteonecrosis
Special Issue Information
Dear Colleagues,
Bones and cartilage, the two most important parts of the musculoskeletal system, provide mobility and maintain the body’s posture. Bone and cartilage damage can have a highly deleterious impact on life and society; therefore, understanding the mechanisms of bone and cartilage regeneration is important. However, questions remain regarding the precise mechanisms of bone formation, how the different molecular processes interact, and the real identity of regenerative cells.
In this Special Issue, we invite researchers and industry experts to submit their original research articles and reviews related to the following topics and keywords:
Bone Morphogenic Proteins (BMPs): The role of BMPs in bone and cartilage regeneration; BMP signaling pathways and their implications in orthopedic therapies; and innovative uses of BMPs in reconstructive surgery.
Bone Union with Cell Therapy: Advances in stem cell therapies for fracture non-union; mechanisms and applications of mesenchymal stem cells (MSCs) in bone healing; comparative studies on bone marrow-derived MSCs versus expanded MSCs; and the role of endothelial progenitor cells (EPCs) in bone regeneration.
Cartilage Regeneration: novel cell-based therapies for cartilage repair; functional of collagen/PLA scaffolds in cartilage regeneration; and BMPs in regulating inflammation and promoting osteogenesis at injured growth plates.
Regeneration and Revascularization of Bone Osteonecrosis: Evaluation of bone turnover markers in MSC-regenerative approaches for osteonecrosis; research studies and reviews in bone regeneration for osteonecrosis patients; and evaluation of osteonecrosis regeneration with MRI and histology, and arteriography.
Cartilage Biomarkers: The identification and validation of new cartilage biomarkers; biomarkers in monitoring the progress of cartilage regeneration therapies; and the role of biochemical markers in the diagnosis and treatment of cartilage damage.
Diaphyseal Regeneration with Callostasis: Techniques and outcomes of callostasis in diaphyseal bone regeneration; the use of BMPs and cell-based therapies in enhancing callostasis; and research studies and reviews on the SECCS system and other scaffold-based therapies.
Union with the Membrane Technique: Advances in the membrane technique for guided bone regeneration; mechanisms and applications of barrier membranes in bone healing; and combination therapies involving BMPs and membranes for optimal bone regeneration.
Submissions should provide novel insights, comprehensive reviews, or significant advancements in the field of bone and cartilage damage and regeneration. We hope that a better understanding of the underlying mechanisms can facilitate the development of new strategies for improving bone and cartilage regeneration.
Prof. Dr. Philippe Hernigou
Guest Editor
Manuscript Submission Information
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Keywords
- bone morphogenic proteins (BMPs)
- bone union with cell therapy
- advances in stem cell therapies for fracture non-union and cartilage regeneration
- novel cell-based therapies for cartilage repair
- functional of collagen/PLA scaffolds in cartilage regeneration
- regeneration and revascularization of bone osteonecrosis
- evaluation of osteonecrosis regeneration with MRI and histology, and arteriography
- identification and validation of new cartilage biomarkers
- diaphyseal regeneration with callostasis
- union with the membrane technique
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Planned Papers
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Comparison of fresh and frozen bone marrow aspirate concentrate cells (BMAC) derived MSCs for in vitro multipotent differentiation and cartilage regeneration in rat OA model
Authors: Xueqin Gao
Affiliation: Steadman Philippon Research Institute (Fort Collins Lab), USA
Title: ACTIVATED CARBON FIBER INCORPORATED WITH METAL IONS: evaluation of biological bone interactions in vitro
Authors: Letícia Cavassini Torquato; Luiz Augusto Rodrigues Santos; Nátaly Domingues Almeida; Clarissa Carvalho Martins Maciel; Glenda Biasotto; Camilla Magnoni Moretto Nunes; Luana Marotta Reis de Vasconcellos; Jossano Saldanha Marcuzzo; Eduardo José de Arruda; Andrea Carvalho De Marco
Affiliation: São Paulo State University (UNESP)
Abstract: The present study had as main objective to evaluate the carbon fiber obtained from textile PAN fiber, in the different forms of presentation: non-activated carbon fiber felt (NACF) and activated carbon fiber felt (ACF) with silver (Ag-ACF), gold (Au-ACF), copper (Cu-ACF), palladium (Pd-ACF) and platinum (Pt-ACF), in the cellular behavior and osteogenesis of mesenchymal cells. Were assessed, cell proliferation, cell viability, quantification of calcium in mineralization nodules, total protein content and alkaline phosphatase activity were quantified, cell interaction by Scanning Electron Microscopy and genotoxicity. All samples were similar in terms of cell proliferation, except for the Ag-ACF group compared to the control group (C). Regarding cell viability, C obtained higher viability than the other groups, and ACF was superior to the Ag-ACF, Cu-ACF, Pt-ACF, being statistically similar to the Au-ACF and Pd-ACF groups. The Au-ACF and Pd-ACF groups presented statistically significant difference to the Ag-ACF and Cu-ACF groups. In the total protein expression Au-ACF had lower expression. For the alkaline phosphatase activity, the Ag-ACF and Cu-ACF showed inferior results. Au-ACF and Pd-ACF have demonstrated potential for future application as scaffolds for bone repair.
Title: Unraveling the mechanism of impaired osteogenic differentiation in osteoporosis: insights from ADRB2 gene polymorphism.
Authors: Olga A. Krasnova; Julia V. Sopova; Anastasiia A. Kovaleva; Polina I. Semenova; Anna S. Zhuk; Daria V. Smirnova; Daria A. Perepletchikova; Vitaly V. Karelkin; Olga M. Lesnyak; Irina E. Neganova
Affiliation: Laboratory of Molecular Science, Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg, Russia
Abstract: Osteoporosis is characterized by increased resorption and decreased bone formation, is pre-dominantly influenced by genetic factors. G-protein coupled receptors (GPCRs) play a vital role in bone homeostasis, and mutations in GPCR genes are associated with osteoporosis. This study aimed to investigate the impact of single nucleotide polymorphism (SNP) rs1042713 in the ADRB2 gene, encoding the beta-2-adrenergic receptor, on osteoblastogenesis. In this study, using quantitative polymerase chain reaction, western immunoblotting, immunofluorescence assays, and flow cytometry, we examined the expression of ADRB2 and markers of bone matrix synthe-sis in mesenchymal stem cells (MSC) derived from osteoporosis patient (OP-MSCs) carrying ADRB2 SNP in comparison with MSCs from healthy donor (HD-MSCs). Results showed signifi-cantly reduced ADRB2 expression in OP-MSCs at both mRNA and protein levels, alongside de-creased TYPE 1 COLLAGEN expression, a key bone matrix component. Notably, OP-MSCs ex-hibited increased ERK kinase expression during differentiation, indicating sustained cell cycle progression unlike to HD-MSC. These results provide novel insights into the association of ADRB2 gene polymorphisms with osteogenic differentiation. The preserved proliferative activity of OP-MSCs with rs1042713 in ADRB2 contributes to their inability to undergo effective osteo-genic differentiation. This research suggests that targeting genetic factors may offer new thera-peutic strategies to mitigate osteoporosis progression.
