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Fermentation
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Antibiotic Production in Streptomyces
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Antibiotic Production in Streptomyces

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Industrial Fermentation".

Deadline for manuscript submissions: closed (31 January 2024) | Viewed by 4410

Special Issue Editors

Prof. Dr. Hang Wu

E-Mail Website
Guest Editor
School of Life Sciences, Anhui University, Hefei, China
Interests: microbial genetics and metabolic engineering; synthetic biology
Prof. Dr. Hua Yuan

E-Mail Website
Guest Editor
College of Life Sciences, Shanghai Normal University, Shanghai, China
Interests: microbial biochemistry and metabolic engineering; synthetic biology

Special Issue Information

Dear Colleagues,

Currently, antibiotics are extensively applied in human medicine, animal health, plant crop protection, etc. Most antibiotics are originally isolated from the fermentation broth of Streptomycetes and related rare actinomycetes as bioactive natural products. The production of these natural products is very complex and depends on a range of different factors, from intracellular gene expression and regulation to extracellular medium ingredients and process control. Over the past decades, a number of Streptomycetes and related rare actinomycetes used for the industrial-scale production of antibiotics have been obtained via multiplex engineering strategies, including random mutagenesis, metabolic engineering, synthetic biology, etc. Engineered strains are further harnessed for high titre production by fermentation optimization. In this Special Issue, we would like to highlight the field of antibiotic production in Streptomycetes and related rare actinomycetes, including but not limited to the following topics：

  • Enhancing antibiotic production in native hosts.
  • Enhancing antibiotic production in heterologous hosts.
  • Enhancing antibiotic production by fermentation optimization.
  • Novel antibiotic discovery in Streptomycetes and related rare actinomycetes.
  • Innovative approach for antibiotic production in Streptomycetes.

Prof. Dr. Hang Wu
Prof. Dr. Hua Yuan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • streptomycetes
  • rare actinomycetes
  • secondary metabolism
  • antibiotic biosynthesis
  • metabolic engineering
  • synthetic biotechnology
  • fermentation engineering
  • bioactive natural product
  • biosynthetic gene cluster
  • genome mining

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Published Papers (2 papers)

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Research

19 pages, 7543 KiB  
Article
Analysis of Secondary Metabolite Synthesis Potential of Streptomyces fradiae sf106 Based on the Whole Genome and Non-Target Metabolomics and Exploration of the Biosynthesis of Tylosin
by Chenbo Jia, Xian Ma, Yuting Jiang, Shanshan Cheng, Sijun Yue and Jianyu Su
Fermentation 2023, 9(10), 866; https://doi.org/10.3390/fermentation9100866 - 25 Sep 2023
Viewed by 1889
Abstract
Streptomyces fradiae sf106 is a type of actinomycete that can produce abundant secondary metabolites, making it a natural cell factory for drug synthesis. In order to comprehensively understand the genomic profile of Streptomyces fradiae sf106 and its potential for producing secondary metabolites, a [...] Read more.
Streptomyces fradiae sf106 is a type of actinomycete that can produce abundant secondary metabolites, making it a natural cell factory for drug synthesis. In order to comprehensively understand the genomic profile of Streptomyces fradiae sf106 and its potential for producing secondary metabolites, a combination of several methods was used to perform whole-genome sequencing of sf106. The results showed that sf106 is most closely related to Streptomyces xinghaiensis S187; the average nucleotide identity and average amino acid identity of sf106 and S187 were more than 96%. The genome size of sf106 is approximately 7300 kb, the GC content is greater than 72%, and more than 6700 coding sequences (CDS) were identified. Analysis of mobile genetic elements revealed the presence of a large number of horizontally transferred genes in Streptomyces fradiae sf106, which contribute to microbial diversity. Through antiSMASH prediction, 22 secondary metabolite gene clusters were obtained, which had great potential to generate polyketide metabolites. By examining the data, it was found that the genes contained in cluster 9 were similar to those involved in tylosin synthesis. Non-targeted metabolome sequencing revealed that a total of 1855 identifiable metabolites were produced in the fermentation broth, and the majority of metabolites showed highly significant differences in mean relative abundance between the groups. The identified metabolites were compared against the KEGG compound database to obtain metabolite classifications, mainly including Biological Roles, Phytochemical Compounds, Lipids, and Pesticides. One-way ANOVA indicated that the relative concentration of tylosin differed significantly across all the growth periods, except for the late-logarithmic and stabilization stages. This study provides important basic information on the secondary metabolite research of sf106, which will help us to understand and apply Streptomyces fradiae sf106 more comprehensively. Full article
(This article belongs to the Special Issue Antibiotic Production in Streptomyces)
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17 pages, 5595 KiB  
Article
Transcriptomics-Guided Investigation of the SLCG_Lrp Regulon Provides New Insights into Its Role for Lincomycin Biosynthesis
by Yurong Xu, Wanlian Xu, Jing Yi, Binglin Li, Meng Liu, Maifei Zhang, Yang Zheng, Ruihua Liu, Hang Wu and Buchang Zhang
Fermentation 2023, 9(4), 396; https://doi.org/10.3390/fermentation9040396 - 19 Apr 2023
Cited by 5 | Viewed by 1574
Abstract
Lincomycin industrially produced by Streptomyces lincolnensis can be adopted to treat infections caused by Gram-positive bacteria. SLCG_Lrp, a transcriptional regulator of the Lrp family, was first identified to positively regulate lincomycin biosynthesis. However, the regulatory role of SLCG_Lrp is yet to be elucidated. [...] Read more.
Lincomycin industrially produced by Streptomyces lincolnensis can be adopted to treat infections caused by Gram-positive bacteria. SLCG_Lrp, a transcriptional regulator of the Lrp family, was first identified to positively regulate lincomycin biosynthesis. However, the regulatory role of SLCG_Lrp is yet to be elucidated. This study utilized RNA-seq for comparing the transcriptome profile of original-strain LCGL and the ΔSLCGL_Lrp mutant. A total of 244 genes comprising 116 downregulated and 128 upregulated genes were differentially expressed between LCGL and ΔSLCGL_Lrp. An in-depth analysis revealed that SLCG_Lrp promotes nitrate assimilation but inhibits fatty acid metabolism, as well as directly regulates five regulators participating in the modulation of multiple cellular processes. With individual inactivation of those regulatory genes in S. lincolnensis LCGL, we confirmed the FadR transcriptional regulator SLCG_2185 was obviously correlated with lincomycin production and found it to transcriptionally stimulate the lincomycin biosynthetic cluster. Furthermore, SLCG_2185 overexpression in the high-yield S. lincolnensis LA219X promoted lincomycin production by 17.8%, and SLCG_2185 being co-overexpressed with SLCG_Lrp in LA219X increased lincomycin production by 28.1% compared to LA219X. Therefore, this investigation not only provides a direction for further investigations regarding the regulation mechanism of SLCG_Lrp, but also provides a basis for guiding the further improvement of lincomycin levels. Full article
(This article belongs to the Special Issue Antibiotic Production in Streptomyces)
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