Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.2
2.8
Journals
Genes
Special Issues
DNA Damage Repair and Plant Stress Response
share announcement

DNA Damage Repair and Plant Stress Response

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Plant Genetics and Genomics".

Deadline for manuscript submissions: closed (25 December 2024) | Viewed by 2493

Special Issue Editor

Dr. Anca Macovei

E-Mail Website
Guest Editor
Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy
Interests: DNA damage response (DDR); microRNAs; abiotic stress response; systems biology; seed biology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Genome preservation is essential for all living organisms. Cells continually undergo DNA damage due to endogenous and exogenous factors, and this can compromise plant growth and development. Understanding how DNA damage response pathways work and how they are regulated can aid in developing plants with multi-stress tolerance. Hence, this is one of the current “hot topics” in addressing challenges that are related to climate change and its effects on plant productivity and food security. Throughout the years, a lot of research has been dedicated to identifying genes/proteins that are involved in DNA repair, and with the aid of cutting-edge biotechnological applications (e.g., omics, genome editing, imaging techniques), this information can be translated into useful application. Therefore, this Special Issue collects articles, such as original research, review, opinion papers, and communications, which broaden knowledge related to DNA damage response, DNA repair, and plant adaptation to climate change.

Dr. Anca Macovei
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biotic and abiotic stresses causing DNA damage
  • crops, wild-type relatives, orphan crops, natural ecosystems
  • DNA damage response (DDR)
  • DDR regulation
  • DNA damage and repair in in vitro tissue culture
  • DNA repair and genome editing tools
  • DNA repair pathways
  • cell-cycle regulation in the context of DDR
  • irradiation treatments in between breeding programs and genomic damage
  • microRNA and DNA repair
  • omics approaches to understanding DDR
  • preservation of genome integrity
  • programmed cell death and endoreduplication
  • reactive oxygen species (ROS) and DNA damage

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

14 pages, 2490 KiB  
Article
Arabidopsis thaliana DNA Damage Response Mutants Challenged with Genotoxic Agents—A Different Experimental Approach to Investigate the TDP1α and TDP1β Genes
by Anna Bertoncini, Paola Pagano and Anca Macovei
Genes 2025, 16(1), 103; https://doi.org/10.3390/genes16010103 - 19 Jan 2025
Viewed by 480
Abstract
Background/Objectives: DNA damage response (DDR) is a highly conserved and complex signal transduction network required for preserving genome integrity. DNA repair pathways downstream of DDR include the tyrosyl-DNA phosphodiesterase1 (TDP1) enzyme that hydrolyses the phosphodiester bond between the tyrosine residue of topoisomerase I [...] Read more.
Background/Objectives: DNA damage response (DDR) is a highly conserved and complex signal transduction network required for preserving genome integrity. DNA repair pathways downstream of DDR include the tyrosyl-DNA phosphodiesterase1 (TDP1) enzyme that hydrolyses the phosphodiester bond between the tyrosine residue of topoisomerase I (TopI) and 3′-phosphate end of DNA. A small TDP1 subfamily, composed of TDP1α and TDP1β, is present in plants. The aim of this work was to investigate the role of the two TDP1 genes in the DDR context. Methods: A series of Arabidopsis thaliana DDR single and double mutants defective in the sog1, e2fb, pol2A, atm, and atr genes, treated with the genotoxic agents camptothecin (CPT, inhibitor of TopI) and NSC120686 (NSC, inhibitor of TDP1), were used. These compounds were specifically used due to their known impact on the TDP1 function. The effect of the treatments was assessed via phenotypic analyses that included germination percentage, speed, and seedling growth. Subsequently, the expression of the TDP1α and TDP1β genes was monitored through qRT-PCR. Results: Overall, the gathered data indicate that the atm mutant was highly sensitive to NSC120686, both phenotypically and concerning the TDP1α gene expression profiles. Alternatively, the upregulation of TDP1β in e2fb, pol2a, and atr supports its implication in the replication stress response. Conclusions: The current study demonstrates that genotoxic stress induced by CPT and NSC has a genotype-dependent effect reflected by a differential expression of TDP1 genes and early phenotypic development. Full article
(This article belongs to the Special Issue DNA Damage Repair and Plant Stress Response)
►▼ Show Figures

Figure 1

17 pages, 3709 KiB  
Article
Cytogenetic and Molecular Effects of Kaolin’s Foliar Application in Grapevine (Vitis vinifera L.) under Summer’s Stressful Growing Conditions
by Ana Carvalho, Lia-Tânia Dinis, Ana Luzio, Sara Bernardo, José Moutinho-Pereira and José Lima-Brito
Genes 2024, 15(6), 747; https://doi.org/10.3390/genes15060747 - 6 Jun 2024
Cited by 2 | Viewed by 1048
Abstract
Grapevine varieties from “Douro Superior” (NE Portugal) experience high temperatures, solar radiation, and water deficit during the summer. This summer’s stressful growing conditions induce nucleic acids, lipids, and protein oxidation, which cause cellular, physiological, molecular, and biochemical changes. Cell cycle anomalies, mitosis delay, [...] Read more.
Grapevine varieties from “Douro Superior” (NE Portugal) experience high temperatures, solar radiation, and water deficit during the summer. This summer’s stressful growing conditions induce nucleic acids, lipids, and protein oxidation, which cause cellular, physiological, molecular, and biochemical changes. Cell cycle anomalies, mitosis delay, or cell death may occur at the cellular level, leading to reduced plant productivity. However, the foliar application of kaolin (KL) can mitigate the impact of abiotic stress by decreasing leaf temperature and enhancing antioxidant defence. Hence, this study hypothesised that KL-treated grapevine plants growing in NE Portugal would reveal, under summer stressful growing conditions, higher progression and stability of the leaf mitotic cell cycle than the untreated (control) plants. KL was applied after veraison for two years. Leaves, sampled 3 and 5 weeks later, were cytogenetically, molecularly, and biochemically analysed. Globally, integrating these multidisciplinary data confirmed the decreased leaf temperature and enhanced antioxidant defence of the KL-treated plants, accompanied by an improved regularity and completion of the leaf cell cycle relative to the control plants. Nevertheless, the KL efficacy was significantly influenced by the sampling date and/or variety. In sum, the achieved results confirmed the hypothesis initially proposed. Full article
(This article belongs to the Special Issue DNA Damage Repair and Plant Stress Response)
►▼ Show Figures

Figure 1

Review

Jump to: Research

22 pages, 955 KiB  
Review
Hallmarks of DNA Damage Response in Germination Across Model and Crop Species
by Federico Sincinelli, Shraddha Shridhar Gaonkar, Sri Amarnadh Gupta Tondepu, Conrado Jr Dueñas and Andrea Pagano
Genes 2025, 16(1), 95; https://doi.org/10.3390/genes16010095 - 17 Jan 2025
Viewed by 591
Abstract
DNA damage response (DDR) contributes to seed quality by guarding genome integrity in the delicate phases of pre- and post-germination. As a key determinant of stress tolerance and resilience, DDR has notable implications on the wider scale of the agroecosystems challenged by harsh [...] Read more.
DNA damage response (DDR) contributes to seed quality by guarding genome integrity in the delicate phases of pre- and post-germination. As a key determinant of stress tolerance and resilience, DDR has notable implications on the wider scale of the agroecosystems challenged by harsh climatic events. The present review focuses on the existing and documented links that interconnect DDR efficiency with an array of molecular hallmarks with biochemical, molecular, and physiological valence within the seed metabolic networks. The expression of genes encoding DDR sensors, transducers, mediators, and effectors is interpreted as a source of conserved hallmarks, along with markers of oxidative damage reflecting the seed’s ability to germinate. Similarly, the accumulation patterns of proteins and metabolites that contribute to DNA stability are predictive of seed quality traits. While a list of candidates is presented from multiple models and crop species, their interaction with chromatin dynamics, cell cycle progression, and hormonal regulation provides further levels of analysis to investigate the seed stress response holistically. The identification of novel hallmarks of DDR in seeds constitutes a framework to prompt validation with different experimental systems, to refine the current models of pre-germinative metabolism, and to promote targeted approaches for seed quality evaluation. Full article
(This article belongs to the Special Issue DNA Damage Repair and Plant Stress Response)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop