Transcriptional Regulation in Plants: From Basic to Applied Research

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (10 September 2022) | Viewed by 8823

Special Issue Editors


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Guest Editor
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 907 36 Umeå, Sweden
Interests: molecular biology; flowering; transcriptional regulation; protein interactome; responses to temperature stress

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Guest Editor
Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy
Interests: DNA damage response (DDR); microRNAs; abiotic stress response; systems biology; seed biology
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Special Issue Information

Dear Colleagues,

Transcriptional regulation governs plant development in response to endogenous and environmental stimuli. Plants evolved several mechanisms to finely orchestrate their genes’ expression in order to adapt their growth to specific environments. The importance of correct transcriptional regulation is intuitive—particularly in plants, in relation to their sessile way of life. This is particularly crucial now, as many ecosystems are subjected to rapid and unpredictable changes causing uncountable damages to local economies.

This Special Issue aims to link the advances of basic research in the transcriptional regulation in model species, particularly those in response to abiotic and biotic stresses, with translational applications to crops and forestry. The idea is to propose a starting point for future applications aimed at developing new plant varieties and resource management to adequately answer to a rapid changing environment. The topics should address, but are not limited to, different aspects of transcriptional regulation related to abiotic and biotic stresses, such as:

  • Gene expression and its regulation;
  • Genome-wide investigation of DNA-binding proteins and their role in transcription;
  • Transcriptomics;
  • Post-transcriptional regulation;
  • Systems biology;
  • Model plants and economically relevant species.

Dr. Silvio Collani
Dr. Anca Macovei
Guest Editors

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Keywords

  • transcriptional regulation
  • plant development
  • biotic stress
  • abiotic stress
  • omics

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Published Papers (2 papers)

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Research

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14 pages, 1876 KiB  
Article
The Specificity of Transgene Suppression in Plants by Exogenous dsRNA
by Konstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Eduard Y. Kostetsky and Alexandra S. Dubrovina
Plants 2022, 11(6), 715; https://doi.org/10.3390/plants11060715 - 8 Mar 2022
Cited by 7 | Viewed by 2693
Abstract
The phenomenon of RNA interference (RNAi) is widely used to develop new approaches for crop improvement and plant protection. Recent investigations show that it is possible to downregulate plant transgenes, as more prone sequences to silencing than endogenous genes, by exogenous application of [...] Read more.
The phenomenon of RNA interference (RNAi) is widely used to develop new approaches for crop improvement and plant protection. Recent investigations show that it is possible to downregulate plant transgenes, as more prone sequences to silencing than endogenous genes, by exogenous application of double-stranded RNAs (dsRNAs) and small interfering RNAs (siRNAs). However, there are scarce data on the specificity of exogenous RNAs. In this study, we explored whether plant transgene suppression is sequence-specific to exogenous dsRNAs and whether similar effects can be caused by exogenous DNAs that are known to be perceived by plants and induce certain epigenetic and biochemical changes. We treated transgenic plants of Arabidopsis thaliana bearing the neomycin phosphotransferase II (NPTII) transgene with specific synthetic NPTII-dsRNAs and non-specific dsRNAs, encoding enhanced green fluorescent protein (EGFP), as well as with DNA molecules mimicking the applied RNAs. None of the EGFP-dsRNA doses resulted in a significant decrease in NPTII transgene expression in the NPTII-transgenic plants, while the specific NPTII-dsRNA significantly reduced NPTII expression in a dose-dependent manner. Long DNAs mimicking dsRNAs and short DNA oligonucleotides mimicking siRNAs did not exhibit a significant effect on NPTII transgene expression. Thus, exogenous NPTII-dsRNAs induced a sequence-specific and RNA-specific transgene-suppressing effect, supporting external application of dsRNAs as a promising strategy for plant gene regulation. Full article
(This article belongs to the Special Issue Transcriptional Regulation in Plants: From Basic to Applied Research)
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Review

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19 pages, 2650 KiB  
Review
Chromatin-Based Transcriptional Reprogramming in Plants under Abiotic Stresses
by Koushik Halder, Abira Chaudhuri, Malik Z. Abdin, Manoj Majee and Asis Datta
Plants 2022, 11(11), 1449; https://doi.org/10.3390/plants11111449 - 29 May 2022
Cited by 13 | Viewed by 4247
Abstract
Plants’ stress response machinery is characterized by an intricate network of signaling cascades that receive and transmit environmental cues and ultimately trigger transcriptional reprogramming. The family of epigenetic regulators that are the key players in the stress-induced signaling cascade comprise of chromatin remodelers, [...] Read more.
Plants’ stress response machinery is characterized by an intricate network of signaling cascades that receive and transmit environmental cues and ultimately trigger transcriptional reprogramming. The family of epigenetic regulators that are the key players in the stress-induced signaling cascade comprise of chromatin remodelers, histone modifiers, DNA modifiers and regulatory non-coding RNAs. Changes in the histone modification and DNA methylation lead to major alterations in the expression level and pattern of stress-responsive genes to adjust with abiotic stress conditions namely heat, cold, drought and salinity. The spotlight of this review falls primarily on the chromatin restructuring under severe abiotic stresses, crosstalk between epigenetic regulators along with a brief discussion on stress priming in plants. Full article
(This article belongs to the Special Issue Transcriptional Regulation in Plants: From Basic to Applied Research)
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