Next Issue
Volume 13, December-1
Previous Issue
Volume 13, November-1
 
 

Plants, Volume 13, Issue 22 (November-2 2024) – 148 articles

  • Issues are regarded as officially published after their release is announced to the table of contents alert mailing list.
  • You may sign up for e-mail alerts to receive table of contents of newly released issues.
  • PDF is the official format for papers published in both, html and pdf forms. To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Reader to open them.
Order results
Result details
Section
Select all
Export citation of selected articles as:
18 pages, 2206 KiB  
Article
Effects of Rocky Desertification Stress on Oat (Avena sativa L.) Seed Germination and Seedling Growth in the Karst Areas of Southwest China
by Haiyan Huang, Yuting Yang, Junqin Li, Yang Gao, Xiangtao Wang, Rui Wang, Zijun Zhou, Puchang Wang and Lili Zhao
Plants 2024, 13(22), 3260; https://doi.org/10.3390/plants13223260 - 20 Nov 2024
Viewed by 381
Abstract
Oat is an important crop widely distributed in temperate zones and is also commonly planted in the karst areas of southwest China. However, due to severe rocky desertification, the complex soil in this area is characterized by high calcium content, alkaline conditions, and [...] Read more.
Oat is an important crop widely distributed in temperate zones and is also commonly planted in the karst areas of southwest China. However, due to severe rocky desertification, the complex soil in this area is characterized by high calcium content, alkaline conditions, and drought, which significantly negatively impact the growth of oat seedlings. To study the adaptability of oats to rocky desertification stress at the seedling stage, we investigated the effects of CaCl2 (0–150 mM), the pH (3–9), and drought stress (PEG-6000 solution at 0 to −0.79 MPa) on seed germination and seedling growth. The results showed that (1) calcium stress had dual effects on seed germination within the range of 5–150 mM CaCl2. Low concentrations of CaCl2 (5 mM) promoted the germination potential, germination rate, germination index, and vigor index of oats, as well as the growth and biomass accumulation of radicles in oat seedlings; however, high concentrations of CaCl2 inhibited these germination parameters. (2) Under drought stress, moderate concentrations of a PEG-6000 solution significantly improved the germination potential and germination rate of oat seeds, but the germination index and vigor index decreased with an increasing PEG-6000 concentration. When the PEG-6000 concentration corresponded to −0.06 MPa, the root growth and fresh weight accumulation of oat seedlings were significantly promoted; however, as the concentration increased to −0.53 MPa and –0.79 MPa, seed germination and seedling growth were significantly inhibited. (3) pH treatments had no significant effect on oat seed germination, but all growth indexes of oats showed a downward trend under alkaline conditions. These results suggest that suitable conditions for oat planting in karst rocky desertification areas are 5 mM CaCl2, pH levels of 5–8, and drought stress between 0 and −0.32 MPa. This study provides a theoretical basis for oat introduction, cultivation, and stress-resistant breeding in this area. Full article
(This article belongs to the Special Issue Ecophysiology and Quality of Crops)
Show Figures

Figure 1

23 pages, 2539 KiB  
Article
Chemical and Biological Properties of Different Romanian Populations of Hyssopus officinalis Correlated via Molecular Docking
by Ilinca Merima Imbrea, Magdalena Osiceanu, Anca Hulea, Mukhtar Adeiza Suleiman, Iuliana Popescu, Doris Floares (Oarga), Emilian Onisan, Alina-Georgeta Neacșu, Cosmin Alin Popescu, Calin Hulea, Georgeta Pop, Simona Niță, Florin Imbrea and Diana Obistioiu
Plants 2024, 13(22), 3259; https://doi.org/10.3390/plants13223259 - 20 Nov 2024
Viewed by 273
Abstract
This study compares three Romanian Hyssopus officinalis species—H. officinalis f. ruber (HOR), H. officinalis f. albus (HOA), and H. officinalis f. cyaneus (HOC)—evaluating their chemical composition and biological activities, specifically protein denaturation, haemolysis inhibition, and antibacterial effects. Chemical profiles were determined using [...] Read more.
This study compares three Romanian Hyssopus officinalis species—H. officinalis f. ruber (HOR), H. officinalis f. albus (HOA), and H. officinalis f. cyaneus (HOC)—evaluating their chemical composition and biological activities, specifically protein denaturation, haemolysis inhibition, and antibacterial effects. Chemical profiles were determined using Gas Chromatography–Mass Spectrometry (GC-MS). The species were cultivated at two distinct locations: the Didactic and Experimental Station DESUSVT and the Agricultural Research and Development Station Lovrin (ARDSL). This study investigates the correlation between chemical composition, biological activities, and local climate data at each site. The results show significant variations in chemical profiles, with species and cultivation location influencing the biological activities. H. officinalis f. albus (HOA) exhibited the strongest antimicrobial activity, particularly against Gram-positive bacteria. The molecular docking analysis highlighted key compounds, such as cyclohexene,4-isopropenyl-1-methoxymethoxymethyl and elemol, with binding solid affinities to microbial and inflammatory proteins. This study provides valuable insights into the chemical and biological properties of Hyssopus officinalis, emphasising its potential in combating microbial infections, protein denaturation, and haemolysis inhibition. Full article
(This article belongs to the Special Issue Chemical Analysis, Bioactivity, and Application of Essential Oils)
Show Figures

Figure 1

23 pages, 5158 KiB  
Article
Development of Analytical Model to Describe Reflectance Spectra in Leaves with Palisade and Spongy Mesophyll
by Ekaterina Sukhova, Yuriy Zolin, Kseniya Grebneva, Ekaterina Berezina, Oleg Bondarev, Anastasiia Kior, Alyona Popova, Daria Ratnitsyna, Lyubov Yudina and Vladimir Sukhov
Plants 2024, 13(22), 3258; https://doi.org/10.3390/plants13223258 - 20 Nov 2024
Viewed by 300
Abstract
Remote sensing plays an important role in plant cultivation and ecological monitoring. This sensing is often based on measuring spectra of leaf reflectance, which are dependent on morphological, biochemical, and physiological characteristics of plants. However, interpretation of the reflectance spectra requires the development [...] Read more.
Remote sensing plays an important role in plant cultivation and ecological monitoring. This sensing is often based on measuring spectra of leaf reflectance, which are dependent on morphological, biochemical, and physiological characteristics of plants. However, interpretation of the reflectance spectra requires the development of new tools to analyze relations between plant characteristics and leaf reflectance. The current study was devoted to the development, parameterization, and verification of the analytical model to describe reflectance spectra of the dicot plant leaf with palisade and spongy mesophyll layers (on the example of pea leaves). Four variables (intensities of forward and backward collimated light and intensities of forward and backward scattered light) were considered. Light reflectance and transmittance on borders of lamina (Snell’s and Fresnel’s laws), light transmittance in the palisade mesophyll (Beer–Bouguer–Lambert law), and light transmittance and scattering in the spongy mesophyll (Kubelka–Munk theory) were described. The developed model was parameterized based on experimental results (reflectance spectra, contents of chlorophylls and carotenoid, and thicknesses of palisade and spongy mesophyll in pea leaves) and the literature data (final R2 was 0.989 for experimental and model-based reflectance spectra). Further model-based and experimental investigations showed that decreasing palisade and spongy mesophyll thicknesses in pea leaves (from 35.5 to 25.2 µm and from 58.6 to 47.8 µm, respectively) increased reflectance of green light and decreased reflectance of near-infrared light. Similarity between model-based and experimental results verified the developed model. Thus, the model can be used to analyze leaf reflectance spectra and, thereby, to increase efficiency of the plant remote and proximal sensing. Full article
(This article belongs to the Special Issue Integration of Spectroscopic and Photosynthetic Analyses in Plants)
Show Figures

Figure 1

18 pages, 2159 KiB  
Article
Effects of Sugarcane/Peanut Intercropping on Root Exudates and Rhizosphere Soil Nutrient
by Xiumei Tang, Lulu Liao, Haining Wu, Jun Xiong, Zhong Li, Zhipeng Huang, Liangqiong He, Jing Jiang, Ruichun Zhong, Zhuqiang Han and Ronghua Tang
Plants 2024, 13(22), 3257; https://doi.org/10.3390/plants13223257 - 20 Nov 2024
Viewed by 232
Abstract
Intercropping can enable more efficient resource use and increase yield. Most current studies focus on the correlation between soil nutrients and crop yield under intercropping conditions. However, the mechanisms related to root exudates and soil nutrients remain unclear. Therefore, this study explored the [...] Read more.
Intercropping can enable more efficient resource use and increase yield. Most current studies focus on the correlation between soil nutrients and crop yield under intercropping conditions. However, the mechanisms related to root exudates and soil nutrients remain unclear. Therefore, this study explored the correlation between rhizosphere soil nutrients and root exudates in sugarcane/peanut intercropping. Root extracts, root exudates, rhizosphere soil enzyme activities, and soil nutrients were analyzed and compared in monocultured and intercropped peanut and sugarcane at different growth stages. The root metabolites were annotated using the Kyoto Encyclopedia of Genes and Genomes pathways to further identify the connection between soil nutrients and root exudates. The effects of intercropping differed in peanut and sugarcane at different growth stages, and the difference between podding and pod-filling stages was significant. Intercropping generally had a great effect on peanut; it not only significantly increased the organic acid, soluble sugars, and phenolic acids in root exudates and extracts from peanuts, but also significantly increased rhizosphere soil enzyme activities and soil nutrient levels. Intercropping peanuts promoted fumaric acid secretion from roots and significantly affected the metabolic pathways of alanine, aspartate, and glutamate. Sugarcane/peanut intercropping can increase root exudates and effectively improve soil nutrients. The changes in soil nutrients are closely related to the effects of fumaric acid on alanine, aspartate, and glutamate metabolism. Full article
(This article belongs to the Section Plant–Soil Interactions)
Show Figures

Figure 1

12 pages, 3511 KiB  
Article
Diversity Patterns of Epiphytic Orchids Along Elevation in the Mountains of Western Nepal
by Manisha Awasthi, Santosh Thapa, Bandana Awasthi, Chae Ryeong Lim, Young Han You and Ki Wha Chung
Plants 2024, 13(22), 3256; https://doi.org/10.3390/plants13223256 - 20 Nov 2024
Viewed by 249
Abstract
The biodiversity and distribution of epiphytic orchids are strongly influenced by their relationship with host plants, and environmental variables like elevation, slope, and local climate are key factors in determining the abundance and diversity of these orchids. The aim of this study was [...] Read more.
The biodiversity and distribution of epiphytic orchids are strongly influenced by their relationship with host plants, and environmental variables like elevation, slope, and local climate are key factors in determining the abundance and diversity of these orchids. The aim of this study was to examine the richness of orchid species at different elevations within the research area of Nepal. Sampling was conducted at elevations ranging from 1300 m to 2800 m above sea level, using a systematic sampling technique known as belt transects. Six circular plots with a diameter of 5.6 m were established in a horizontal transect at each site, spaced at least 50 m apart, with 100 elevation interval. The analysis revealed a hump-shaped relationship between orchid species richness and elevation, with the highest species richness observed at altitude of 2100–2200 m. The abundance of orchids was significantly correlated with host characteristics, including habit (shrub/tree), bark texture, nature (deciduous/evergreen), and physical factors. This study underscores the significant contribution of host characteristics and environmental factors in explaining the diversity of epiphytic orchid species along the elevation of the Himalayas. Full article
(This article belongs to the Special Issue Orchid Conservation and Biodiversity)
Show Figures

Figure 1

47 pages, 3429 KiB  
Review
Recent Advances in Postharvest Application of Exogenous Phytohormones for Quality Preservation of Fruits and Vegetables
by Sbulelo Mwelase, Jerry O. Adeyemi and Olaniyi A. Fawole
Plants 2024, 13(22), 3255; https://doi.org/10.3390/plants13223255 - 20 Nov 2024
Viewed by 308
Abstract
The increasing global population has heightened the demand for food, leading to escalated food production and, consequently, the generation of significant food waste. Factors such as rapid ripening, susceptibility to physiological disorders, and vulnerability to microbial attacks have been implicated as contributing to [...] Read more.
The increasing global population has heightened the demand for food, leading to escalated food production and, consequently, the generation of significant food waste. Factors such as rapid ripening, susceptibility to physiological disorders, and vulnerability to microbial attacks have been implicated as contributing to the accelerated senescence associated with food waste generation. Fruits and vegetables, characterized by their high perishability, account for approximately half of all food waste produced, rendering them a major area of concern. Various postharvest technologies have thus been employed, including the application of phytohormone treatments, to safeguard and extend the storability of highly perishable food products. This review, therefore, explores the physicochemical properties and biological aspects of phytohormones that render them suitable for food preservation. Furthermore, this review examines the effects of externally applied phytohormones on the postharvest physiology and quality attributes of fresh produce. Finally, the review investigates the mechanisms by which exogenous phytohormones preserve food quality and discusses the associated limitations and safety considerations related to the use of these compounds in food applications. Full article
(This article belongs to the Special Issue Postharvest Quality and Physiology of Vegetables and Fruits)
Show Figures

Figure 1

18 pages, 5996 KiB  
Article
Contrasting Weather and Stocking Effects on Eucalyptus Initial Coppice Response in Brazil
by Pietro Gragnolati Fernandes, Clayton Alcarde Alvares, Túlio Barroso Queiroz, Pedro Vitor Pimenta, Jarbas Silva Borges, James Stahl, Flávio Teixeira Mendes, Amanda Souza, Gustavo Matheus Silva, Gualter Guenther Costa da Silva, Sara Bezerra Bandeira Milhomem, Rosilvam Ramos de Sousa and Rodrigo Eiji Hakamada
Plants 2024, 13(22), 3254; https://doi.org/10.3390/plants13223254 - 20 Nov 2024
Viewed by 309
Abstract
In Eucalyptus plantations, coppice rotations often yield less than initial rotations. The TECHS project (Tolerance of Eucalyptus Clones to Hydric, Thermal and Biotic Stresses) studied short rotation coppicing across a 3000 km gradient. The main objective of this work was to compare the [...] Read more.
In Eucalyptus plantations, coppice rotations often yield less than initial rotations. The TECHS project (Tolerance of Eucalyptus Clones to Hydric, Thermal and Biotic Stresses) studied short rotation coppicing across a 3000 km gradient. The main objective of this work was to compare the survival, sprouting, and initial growth of Eucalyptus clones managed and to examine factors that might influence the productivity of the coppice rotation: climate, genotypes, and stocking. Eight of the TECHS sites spread from latitudes 6° S to 30° S were included in the coppice study, with 17 genotypes at each site. The initial rotation had been planted at a 3 m × 3 m spacing and also in a spacing trial at densities from 500 to 3500 trees ha−1. Six months after harvesting the initial Eucalyptus rotation, average survival was 88%, with tropical clones showing over twice the sprouting biomass (6.7 vs. 2.9 Mg ha−1) and four times the woody biomass compared to subtropical clones (4.7 vs. 1.1 Mg ha−1). Greater initial water deficits had stronger sprouting and growth. Clones with higher belowground carbon allocation in the initial rotation performed better in coppicing, and precipitation became more influential after 12 months. Drought and spacing trials significantly affected growth. Full article
Show Figures

Figure 1

19 pages, 29968 KiB  
Article
Ripe Tomato Detection Algorithm Based on Improved YOLOv9
by Yan Wang, Qianjie Rong and Chunhua Hu
Plants 2024, 13(22), 3253; https://doi.org/10.3390/plants13223253 - 20 Nov 2024
Viewed by 257
Abstract
Recognizing ripe tomatoes is a crucial aspect of tomato picking. To ensure the accuracy of inspection results, You Only Look Once version 9 (YOLOv9) has been explored as a fruit detection algorithm. To tackle the challenge of identifying tomatoes and the low accuracy [...] Read more.
Recognizing ripe tomatoes is a crucial aspect of tomato picking. To ensure the accuracy of inspection results, You Only Look Once version 9 (YOLOv9) has been explored as a fruit detection algorithm. To tackle the challenge of identifying tomatoes and the low accuracy of small object detection in complex environments, we propose a ripe tomato recognition algorithm based on an enhanced YOLOv9-C model. After collecting tomato data, we used Mosaic for data augmentation, which improved model robustness and enriched experimental data. Improvements were made to the feature extraction and down-sampling modules, integrating HGBlock and SPD-ADown modules into the YOLOv9 model. These measures resulted in high detection performance with precision and recall rates of 97.2% and 92.3% in horizontal and vertical experimental comparisons, respectively. The module-integrated model improved accuracy and recall by 1.3% and 1.1%, respectively, and also reduced inference time by 1 ms compared to the original model. The inference time of this model was 14.7 ms, which is 16 ms better than the RetinaNet model. This model was tested accurately with [email protected] (%) up to 98%, which is 9.6% higher than RetinaNet. Its increased speed and accuracy make it more suitable for practical applications. Overall, this model provides a reliable technique for recognizing ripe tomatoes during the picking process. Full article
Show Figures

Figure 1

19 pages, 1697 KiB  
Article
The Molecular Cloning and Functional Analysis of the FAD2 Gene in Hippophe rhamnoids L.
by Di Cong, Chang Ni, Luwen Han, Jianlin Cheng, Wei An, Siyu An, Hongzhang Liu, Huijing Liu, Dan Yao, Yuqin Fu, Shuying Liu and Guoshuang Chen
Plants 2024, 13(22), 3252; https://doi.org/10.3390/plants13223252 - 20 Nov 2024
Viewed by 279
Abstract
Seabuckthorn (Hippophae rhamnoides Linn.) is a commonly utilized medicinal crop with various applications in the treatment of different diseases. Two particularly noteworthy nutrients in seabuckthorn fruit are seabuckthorn oil and flavonoids. In recent years, seabuckthorn oil has attracted considerable attention due to [...] Read more.
Seabuckthorn (Hippophae rhamnoides Linn.) is a commonly utilized medicinal crop with various applications in the treatment of different diseases. Two particularly noteworthy nutrients in seabuckthorn fruit are seabuckthorn oil and flavonoids. In recent years, seabuckthorn oil has attracted considerable attention due to its perceived benefits for beauty and healthcare. Consequently, there is a clear need for further research into seabuckthorn oil. While numerous studies have been conducted on the regulation of oil by the FAD2 gene family, there is a paucity of literature examining the molecular mechanism of FAD2 gene involvement in seabuckthorn oil regulation. Accordingly, two FAD2 genes have been identified in seabuckthorn, which are classified differently and perform distinct functions. Both genes are located in the endoplasmic reticulum. Following transient expression in seabuckthorn fruits, it was observed that HrFAD2-1 and HrFAD2-3 were capable of influencing the synthesis of α-linolenic acid, with HrFAD2-1 additionally demonstrated to facilitate the synthesis of lysophosphatidic acid. All of the aforementioned genes have been observed to promote jasmonic acid (JA) synthesis. The heterologous transformation of Linum usitatissimum demonstrates that both HrFAD2-1 and HrFAD2-3 are capable of promoting plant growth. The HrFAD2-1 gene was observed to significantly increase the content of major fatty acids in Linum usitatissimum Linn seeds, whereas the HrFAD2-3 gene appeared to be primarily involved in the regulation of plant growth and development. In conclusion, a preliminary investigation into the functions of the HrFAD2-1 and HrFAD2-3 genes in fatty acid synthesis was conducted. This revealed that HrFAD2-1 is closely associated with oleic acid synthesis and acts as a negative regulator. Furthermore, our findings will provide a foundation for subsequent investigations into the fatty acid synthesis pathway in Hippophae rhamnoides oil, offering a theoretical basis for subsequent studies at the molecular level. Full article
Show Figures

Figure 1

12 pages, 1499 KiB  
Article
Histochemical Localization and Cytotoxic Potential of Alkaloids in Phaedranassa lehmannii
by Lina M. Trujillo Chacón, Hawer Leiva, José M. Rojas, Isabel C. Zapata Vahos, Dagoberto Castro, María Domínguez and Edison Osorio
Plants 2024, 13(22), 3251; https://doi.org/10.3390/plants13223251 - 20 Nov 2024
Viewed by 278
Abstract
Plants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in [...] Read more.
Plants of the subfamily Amaryllidoideae are a source of unique and bioactive alkaloids called Amaryllidaceae alkaloids. The study of their anticancer potential has intensified in recent years. This work aims to locate and characterize the profile of cytotoxic alkaloids biosynthesized and stored in different tissues of Phaedranassa lehmannii Regel using different histochemical methods and chromatographic analysis. The histochemical analysis in the bulbs revealed the presence of alkaloids at the basal edge of the scale-like leaves and bud apical zone. The GC-MS analysis indicated that the bulbs biosynthesize crinane- (9.80 µg/g DW), galanthamine- (8.04 µg/g DW), lycorine- (7.38 µg/g DW), and narciclasine-type (3.75 µg/g DW) alkaloids. The root biosynthesizes alkaloids that are mainly distributed mostly in lycorine- (225.29 µg/g DW) and galanthamine-type (72.35 µg/g DW) alkaloids. The total alkaloids biosynthesized by the root (324.93 µg/g DW) exceeded eleven times the abundance of the alkaloids identified in the bulbs (28.97 µg/g DW). In addition, the total alkaloid fractions exhibited a dose-dependent cytotoxic effect in the evaluated concentrations, with IC50 values of 11.76 ± 0.99 µg/mL and 2.59 ± 0.56 µg/mL against human lung (A549) cancer cells and 8.00 ± 1.35 µg/mL and 18.74 ± 1.99 µg/mL against gastric (AGS) cancer cells. The present study provided evidence to locate and characterize the alkaloids of P. lehmannii grown under nursery conditions as a species producing potential antiproliferative alkaloids. Full article
(This article belongs to the Special Issue Alkaloids: Chemical Structures with Pharmaceutical Potential)
Show Figures

Figure 1

17 pages, 3917 KiB  
Article
Efficiency of Desiccation, Biomass Production, and Nutrient Accumulation in Zuri and Quênia Guinea Grasses in Integrated Crop–Livestock Systems and Second-Crop Maize
by Bruno de Souza Marques, Kátia Aparecida de Pinho Costa, Hemython Luís Bandeira do Nascimento, Ubirajara Oliveira Bilego, Eduardo Hara, Rose Luiza Moraes Tavares, Juliana Silva Rodrigues Cabral, Luciana Maria da Silva, José Carlos Bento, Breno Furquim de Morais, Adriano Carvalho Costa and Tiago do Prado Paim
Plants 2024, 13(22), 3250; https://doi.org/10.3390/plants13223250 - 20 Nov 2024
Viewed by 476
Abstract
Modern agriculture faces the challenge of increasing production without expanding cultivated areas, promoting sustainable practices that ensure food security and environmental preservation. Integrated crop–livestock systems (ICLSs) stand out as an effective strategy, diversifying and intensifying agricultural production in a sustainable manner, ensuring adequate [...] Read more.
Modern agriculture faces the challenge of increasing production without expanding cultivated areas, promoting sustainable practices that ensure food security and environmental preservation. Integrated crop–livestock systems (ICLSs) stand out as an effective strategy, diversifying and intensifying agricultural production in a sustainable manner, ensuring adequate soil cover, and improving nutrient cycling efficiency. Thus, this study aimed to explore and compare integrated crop–livestock systems with Zuri guinea grass (Panicum maximum cv. BRS Zuri) and Quênia guinea grass (Panicum maximum cv. BRS Quênia) against the conventional soybean/maize succession method in a tropical region, and how these systems affect biomass decomposition, C:N ratio, nutrient cycling, and fertilizer equivalents. A field experiment was conducted in two phases: the first in the second-crop season and the second in the main season, using a randomized block design with four replicates. The treatments consisted of two ICLS systems, one with Zuri and Quênia guinea grasses established after soybean, and a succession system with maize established after soybean. The results indicated that Quênia guinea grass showed greater desiccation efficiency, with an injury rate of 86.5% at 21 days, 8.5% higher compared to Zuri guinea grass. In terms of biomass, Zuri and Quênia guinea grasses had average productions of 7021.1 kg ha−1, which was 43.25% higher compared to maize biomass. The biomass decomposition of the grasses was faster due to their lower C:N ratio, resulting in greater nutrient release into the soil. Both forage grasses (Zuri and Quênia guinea grasses) are suitable for integrated crop–livestock systems, as they showed similar biomass production and nutrient accumulation. Soybean yield was not influenced by the different cropping systems, showing similar results between the biomass of Zuri and Quênia guinea grasses and maize. However, grass biomass enriches the soil more through the return of fertilizer equivalents, which in future studies could be considered for the reduction of mineral fertilizers, ensuring greater sustainability of agricultural systems. Full article
(This article belongs to the Special Issue Ecophysiology and Quality of Crops)
Show Figures

Figure 1

17 pages, 4865 KiB  
Article
Morpho-Phenological, Chemical, and Genetic Characterization of Italian Maize Landraces from the Lazio Region
by Rita Redaelli, Laura Bassolino, Carlotta Balconi, Irma Terracciano, Alessio Torri, Federica Nicoletti, Gianluca Benedetti, Valentina Iacoponi, Roberto Rea and Paola Taviani
Plants 2024, 13(22), 3249; https://doi.org/10.3390/plants13223249 - 20 Nov 2024
Viewed by 353
Abstract
In the framework of a Collaboration Agreement between CREA and ARSIAL, a morpho-phenological, chemical, and genetic characterization of maize populations native to the Lazio region was carried out. During 2022 and 2023, a set of 50 accessions, belonging both to ARSIAL and CREA [...] Read more.
In the framework of a Collaboration Agreement between CREA and ARSIAL, a morpho-phenological, chemical, and genetic characterization of maize populations native to the Lazio region was carried out. During 2022 and 2023, a set of 50 accessions, belonging both to ARSIAL and CREA maize collections, were multiplied in Bergamo. Morpho-phenological descriptors were recorded in the field: plant height, ear height, and male and female flowering time. The grain chemical composition in terms of protein, lipid, starch, ash and fiber was evaluated by near-infrared spectroscopy (NIRS). A double-digest restriction-site-associated DNA sequencing (ddRADseq) strategy was used to genotype the landraces. The two collections were not significantly different in terms of grain chemical composition. On the other hand, the ARSIAL and CREA germplasm showed a different distribution in the three cluster-based population structure obtained by ddRADseq, which largely corresponded to the distribution map of their collection sites. The materials from the Lazio region maintained by ARSIAL and CREA were revealed to be different. The comparison between the two groups of landraces showed the importance of characterizing germplasm collections to promote the recovery and valorization of local biodiversity. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
Show Figures

Figure 1

15 pages, 3542 KiB  
Article
Genome-Wide Identification and Characterization of SNPs and InDels of Capsicum annuum var. glabriusculum from Mexico Based on Whole Genome Sequencing
by Valeria Itzel Moreno-Contreras, Ma. Carmen E. Delgado-Gardea, Jesús A. Ramos-Hernández, Alfonso Mendez-Tenorio, Hugo Varela-Rodríguez, Blanca Sánchez-Ramírez, Zilia Y. Muñoz-Ramírez and Rocío Infante-Ramírez
Plants 2024, 13(22), 3248; https://doi.org/10.3390/plants13223248 - 20 Nov 2024
Viewed by 384
Abstract
Capsicum annuum var. glabriusculum is an economically important horticultural crop and is considered the wild genetic ancestor of chili peppers. The distribution range extends from southern North America, through Central America, to South America. Approximately 226 million 150 paired-end reads were generated from [...] Read more.
Capsicum annuum var. glabriusculum is an economically important horticultural crop and is considered the wild genetic ancestor of chili peppers. The distribution range extends from southern North America, through Central America, to South America. Approximately 226 million 150 paired-end reads were generated from CHMX_Ch1 (a C. annuum from Chihuahua, Mexico). To compare with the CHMX_Ch1 genome, high-quality reads from QO (a C. annuum from Querétaro, Mexico) were downloaded from the NCBI database. A total of 210,324 variants were detected in CHMX_Ch1, whereas 169,718 variants were identified in QO, all compared to the domesticated C. annuum reference genome, UCD10Xv1.1. This comprised 203,990 SNPs and 6334 InDels in CHMX_Ch1 and 164,955 SNPs and 4763 InDels in QO. The variants with high and moderate impact were identified as missense, splice acceptor, splice donor, start lost, stop gain, stop lost, frameshift, insertion, and deletion effects. The candidate genes with the highest fold enrichment values among the SNPs were predominantly involved in gene regulation and metabolic processes. InDels were associated with nuclear and transcriptional regulator activity in both genomes. Overall, a greater number of variants were found in CHMX_Ch1 compared to QO. This study provides knowledge of the principal functions associated with high- and moderate-impact variants and supplies a resource for further investigations of the genetic characteristics of these chiltepin peppers. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
Show Figures

Figure 1

28 pages, 2364 KiB  
Review
Optimizing Brassica oleracea L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration
by Miriam Romero-Muñoz and Margarita Pérez-Jiménez
Plants 2024, 13(22), 3247; https://doi.org/10.3390/plants13223247 - 19 Nov 2024
Viewed by 582
Abstract
The Brassica oleracea L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic [...] Read more.
The Brassica oleracea L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic hybridization to ease hybrid seed production. Protoplasts entail a versatile tool in plant biotechnology, supporting breeding strategies that involve genome editing and hybridization. This review discusses the use of somatic hybridization in B. oleracea L. as a biotechnological method for developing fusion products with desirable agronomic traits, particularly cytoplasmic male sterile (CMS) condition. These CMS lines are critical for implementing a cost-effective, efficient, and reliable system for producing F1 hybrids. We present recent studies on CMS systems in B. oleracea L. crops, providing an overview of established models that explain the mechanisms of CMS and fertility restoration. Additionally, we emphasize key insights gained from protoplast fusion applied to B. oleracea L. breeding. Key steps including pre-treatments of donor plants, the main tissues used as sources of parental protoplasts, methods for obtaining somatic hybrids and cybrids, and the importance of establishing a reliable plant regeneration method are discussed. Finally, the review explores the incorporation of genome editing technologies, such as CRISPR-Cas9, to introduce multiple agronomic traits in Brassica species. This combination of advanced biotechnological tools holds significant promise for enhancing B. oleracea breeding programs in the actual climate change context. Full article
Show Figures

Figure 1

15 pages, 6238 KiB  
Article
Photosynthetic Responses of Peat Moss (Sphagnum spp.) and Bog Cranberry (Vaccinium oxycoccos L.) to Spring Warming
by Michal Antala, Abdallah Yussuf Ali Abdelmajeed, Marcin Stróżecki, Włodzimierz Krzesiński, Radosław Juszczak and Anshu Rastogi
Plants 2024, 13(22), 3246; https://doi.org/10.3390/plants13223246 - 19 Nov 2024
Viewed by 286
Abstract
The rising global temperature makes understanding the impact of warming on plant physiology in critical ecosystems essential, as changes in plant physiology can either help mitigate or intensify climate change. The northern peatlands belong to the most important parts of the global carbon [...] Read more.
The rising global temperature makes understanding the impact of warming on plant physiology in critical ecosystems essential, as changes in plant physiology can either help mitigate or intensify climate change. The northern peatlands belong to the most important parts of the global carbon cycle. Therefore, knowledge of the ongoing and future climate change impacts on peatland vegetation photosynthesis is crucial for further refinement of peatland or global carbon cycle and vegetation models. As peat moss (Sphagnum spp.) and bog cranberry (Vaccinium oxycoccos L.) represent some of the most common plant functional groups of peatland vegetation, we examined the impact of experimental warming on the status of their photosynthetic apparatus during the early vegetation season. We also studied the differences in the winter to early spring transition of peat moss and bog cranberry photosynthetic activity. We have shown that peat moss starts photosynthetic activity earlier because it relies on light-dependent energy dissipation through the winter. However, bog cranberry needs a period of warmer temperature to reach full activity due to the sustained, non-regulated, heat dissipation during winter, as suggested by the doubling of photosystem II efficiency and 36% decrease in sustained heat dissipation between the mid-March and beginning of May. The experimental warming further enhanced the performance of photosystem II, indicated by a significant increase in the photosystem II performance index on an absorption basis due to warming. Therefore, our results suggest that bog cranberry can benefit more from early spring warming, as its activity is sped up more compared to peat moss. This will probably result in faster shrub encroachment of the peatlands in the warmer future. The vegetation and carbon models should take into account the results of this research to predict the peatland functions under changing climate conditions. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
Show Figures

Figure 1

23 pages, 960 KiB  
Article
Genome-Wide Identification and Stress Responses of Cowpea Thaumatin-like Proteins: A Comprehensive Analysis
by Carolline de Jesús-Pires, José Ribamar Costa Ferreira-Neto, Roberta Lane de Oliveira-Silva, Jéssica Barboza da Silva, Manassés Daniel da Silva, Antônio Félix da Costa and Ana Maria Benko-Iseppon
Plants 2024, 13(22), 3245; https://doi.org/10.3390/plants13223245 - 19 Nov 2024
Viewed by 306
Abstract
Cowpea (Vigna unguiculata (L.) Walp.) is an important legume cultivated mainly in regions with limited water availability across the African and American continents. Its productivity is significantly affected by environmental stresses. Thaumatin-like proteins (TLPs), which belong to the PR-5 (pathogenesis-related 5) protein family, [...] Read more.
Cowpea (Vigna unguiculata (L.) Walp.) is an important legume cultivated mainly in regions with limited water availability across the African and American continents. Its productivity is significantly affected by environmental stresses. Thaumatin-like proteins (TLPs), which belong to the PR-5 (pathogenesis-related 5) protein family, are known to be responsive to both biotic and abiotic stresses. However, their role remains controversial, with some TLPs associated with plant defense (particularly against fungal infections) and others associated with abiotic stresses response. In this study, we evaluated the structural diversity and gene expression of TLPs in cowpea (VuTLPs) under different stress conditions, including biotic [mechanical injury followed by inoculation with Cowpea Aphid-borne Mosaic Virus (CABMV) or Cowpea Severe Mosaic Virus (CPSMV)] and abiotic (root dehydration). Genomic anchoring of VuTLPs revealed 34 loci encoding these proteins. Neighbor- joining analysis clustered the VuTLPs into three distinct groups. We identified 15 segmental duplication and 6 tandem duplication gene pairs, with the majority of VuTLP genes found to be under purifying selection. Promoter analysis associated VuTLPs with bHLH, Dof-type, and MYB- related transcription factors, supporting their diverse roles. Diversity in VuTLP function was also observed in their expression profiles under the studied stress conditions. Gene expression data showed that most VuTLPs are recruited within the first minutes after biotic stress imposition. For the root dehydration assay, the most transcripts were up-regulated 150 min post-stress. Moreover, the gene expression data suggested that VuTLPs exhibit functional specialization depending on the stress condition, highlighting their diverse roles and biotechnological potential. Full article
Show Figures

Figure 1

21 pages, 2975 KiB  
Article
Patterns and Mechanisms of Legume Responses to Nitrogen Enrichment: A Global Meta-Analysis
by Juan Tang, Wei Li, Ting Wei, Ruilong Huang and Zhuanfei Zeng
Plants 2024, 13(22), 3244; https://doi.org/10.3390/plants13223244 - 19 Nov 2024
Viewed by 319
Abstract
Nitrogen (N), while the most abundant element in the atmosphere, is an essential soil nutrient that limits plant growth. Leguminous plants naturally possess the ability to fix atmospheric nitrogen through symbiotic relationships with rhizobia in their root nodules. However, the widespread use of [...] Read more.
Nitrogen (N), while the most abundant element in the atmosphere, is an essential soil nutrient that limits plant growth. Leguminous plants naturally possess the ability to fix atmospheric nitrogen through symbiotic relationships with rhizobia in their root nodules. However, the widespread use of synthetic N fertilizers in modern agriculture has led to N enrichment in soils, causing complex and profound effects on legumes. Amid ongoing debates about how leguminous plants respond to N enrichment, the present study compiles 2174 data points from 162 peer-reviewed articles to analyze the impacts and underlying mechanisms of N enrichment on legumes. The findings reveal that N enrichment significantly increases total legume biomass by 30.9% and N content in plant tissues by 13.2% globally. However, N enrichment also leads to notable reductions, including a 5.8% decrease in root-to-shoot ratio, a 21.2% decline in nodule number, a 29.3% reduction in nodule weight, and a 27.1% decrease in the percentage of plant N derived from N2 fixation (%Ndfa). Legume growth traits and N2-fixing capability in response to N enrichment are primarily regulated by climatic factors, such as mean annual temperature (MAT) and mean annual precipitation (MAP), as well as the aridity index (AI) and N fertilizer application rates. Correlation analyses show that plant biomass is positively correlated with MAT, and tissue N content also exhibits a positive correlation with MAT. In contrast, nodule numbers and tissue N content are negatively correlated with N fertilizer application rates, whereas %Ndfa shows a positive correlation with AI and MAP. Under low N addition, the increase in total biomass in response to N enrichment is twice as large as that observed under high N addition. Furthermore, regions at lower elevations with abundant hydrothermal resources are especially favorable for total biomass accumulation, indicating that the responses of legumes to N enrichment are habitat-specific. These results provide scientific evidence for the mechanisms underlying legume responses to N enrichment and offer valuable insights and theoretical references for the conservation and management of legumes in the context of global climate change. Full article
(This article belongs to the Special Issue Fertilizer and Abiotic Stress)
Show Figures

Figure 1

12 pages, 1728 KiB  
Article
Deodeokaloid, a New Indole Alkaloid N-Glycoside and Bioactive Phenolic Compounds from the Roots of Codonopsis lanceolata
by Yeo Rang Cho, Joo-Hyun Hong, Dong-Min Kang, Yoon-Joo Ko, Mi-Jeong Ahn and Ki Hyun Kim
Plants 2024, 13(22), 3243; https://doi.org/10.3390/plants13223243 - 19 Nov 2024
Viewed by 303
Abstract
Codonopsis lanceolata, commonly known as the bonnet bellflower or deodeok, is primarily found in Eastern Asia. Its roots have been used traditionally across Asia to treat various ailments such as bronchitis, coughs, asthma, and inflammation. In our ongoing efforts to discover bioactive [...] Read more.
Codonopsis lanceolata, commonly known as the bonnet bellflower or deodeok, is primarily found in Eastern Asia. Its roots have been used traditionally across Asia to treat various ailments such as bronchitis, coughs, asthma, and inflammation. In our ongoing efforts to discover bioactive natural products, a phytochemical investigation of the n-BuOH fraction of C. lanceolata root extracts led to the isolation and identification of a new indole alkaloid N-glycoside, deodeokaloid (D-indole-3-lactic acid N-β-D-glucopyranoside) (1), alongside known compounds tangshenoside I (2), tangshenoside IV (3), and chlorogenic acid (4) through HPLC purification. The structure of the new compound 1 was elucidated using 1D and 2D NMR spectroscopy and high-resolution electrospray ionization mass spectrometry (HR-ESIMS). Its absolute configuration was determined through a combination of DP4+ probability analysis and chemical reactions. The isolated compounds 14 were evaluated for their anti-Helicobacter pylori and antioxidant activities. In the anti-H. pylori assay, compound 3 showed antibacterial activity similar to that of quercetin as the positive control, inhibiting the bacterial growth by 36.8%. Compound 4 exhibited the most potent antioxidant activity, with an ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)] radical scavenging activity of 1624.7 mmol TE/mol and a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging capacity of 707.5 mmol Trolox equivalent (TE)/mol. Compounds 24 displayed significant intracellular reactive oxygen species (ROS) scavenging capacity in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. This study highlights C. lanceolata roots as a promising natural source of bioactive compounds with potential therapeutic applications. Full article
(This article belongs to the Special Issue Alkaloids: Chemical Structures with Pharmaceutical Potential)
Show Figures

Figure 1

13 pages, 3317 KiB  
Article
Induction and Transcriptome Analysis of Callus Tissue from Endosperm of Makapuno Coconut
by Jing Huang, Zijia Liu, Qinghui Guo, Jixin Zou, Yusheng Zheng and Dongdong Li
Plants 2024, 13(22), 3242; https://doi.org/10.3390/plants13223242 - 19 Nov 2024
Viewed by 304
Abstract
The makapuno coconut endosperm is distinguished by its soft and irregular texture, in contrast to the solid endosperm of regular coconuts. To establish a scientific foundation for studying makapuno coconuts, callus was induced from makapuno endosperm using a combination of plant growth regulators. [...] Read more.
The makapuno coconut endosperm is distinguished by its soft and irregular texture, in contrast to the solid endosperm of regular coconuts. To establish a scientific foundation for studying makapuno coconuts, callus was induced from makapuno endosperm using a combination of plant growth regulators. The induction was successful, and the resulting callus was subsequently subcultured for further study. Transcriptome sequencing of the makapuno callus identified 429 differentially expressed genes (DEGs), with 273 upregulated and 156 downregulated, compared to callus derived from regular coconut endosperm. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that these DEGs were involved in key metabolic pathways, including fructose and mannose metabolism, carbon fixation in photosynthetic organisms, galactose metabolism, and amino sugar and nucleotide sugar metabolism. Furthermore, lipid content analysis of the makapuno callus revealed a significantly higher total lipid level compared to regular callus, with notable differences in the levels of specific fatty acids, such as myristic acid, palmitic acid, and linoleic acid. This study establishes a novel platform for molecular biological research on makapuno coconuts and provides valuable insights into the molecular mechanisms underlying the formation of makapuno callus tissue. The findings also lay the groundwork for future research aimed at elucidating the unique properties of makapuno endosperm and exploring its potential applications. Full article
(This article belongs to the Special Issue Genetic Improvement in Coconut)
Show Figures

Figure 1

14 pages, 6573 KiB  
Article
Threshold Behavior Hidden in the Growth Response of Peat Moss Sphagnum riparium to Temperature
by Victor L. Mironov
Plants 2024, 13(22), 3241; https://doi.org/10.3390/plants13223241 - 19 Nov 2024
Viewed by 269
Abstract
The balance between photosynthetic carbon accumulation and respiratory loss in plants varies depending on temperature. This leads to a situation where the increased need for carbon is not met when a certain temperature threshold is reached. Over the last two decades, temperature thresholds [...] Read more.
The balance between photosynthetic carbon accumulation and respiratory loss in plants varies depending on temperature. This leads to a situation where the increased need for carbon is not met when a certain temperature threshold is reached. Over the last two decades, temperature thresholds in carbon metabolism in autotrophic systems have been widely studied. However, it remains unclear how these thresholds manifest themselves in the natural growth of individual plant species. To address this issue, we used data from an extensive monitoring of the growth of peat moss Sphagnum riparium over 9 years in mires in Karelia (Russia). We measured the growth of shoots in sample plots and obtained 1609 estimates of growth rates during the monitoring period. Investigating the relationship between growth rate and temperature, we identified two distinct intervals in response to temperature. These two intervals are separated by the temperature threshold of 13.2 °C. The first interval, which covers 42% of the growing season, exhibits a strong exponential dependence of growth rate on temperature, with a coefficient Q10 = 4.01. This indicates that growth is most sensitive to changes in temperature within this range. In contrast, the second interval (58% of the growing season) shows a weaker dependence, with a Q10 coefficient of 1.21, suggesting that growth is less responsive to changes within this temperature range. The temperature threshold was found to be negatively related to May (r = −0.76; p = 0.018) and September (r = −0.78; p = 0.012) temperatures of the previous growing season, and together they best explain (r = −0.91; p = 0.0007) the temperature threshold. Overall, our findings suggest that the temperature threshold does exist in the growth of S. riparium and can be identified in different years. The negative correlation between temperature threshold and May and September temperatures from the previous year indicates that intervals in the growing season with temperatures near the temperature threshold have an impact on subsequent carbon balance and are particularly significant for the further growth and development of Sphagnum mosses. Full article
(This article belongs to the Special Issue Bryophyte Biology)
Show Figures

Figure 1

15 pages, 1001 KiB  
Article
Assessing the Host Range of Ophraella communa for the Biological Control of Ambrosia artemisiifolia in France
by Zoé Rousset, Alberto Zamprogna, Coline C. Jaworski, Nicolas Desneux and Vincent Lesieur
Plants 2024, 13(22), 3240; https://doi.org/10.3390/plants13223240 - 19 Nov 2024
Viewed by 330
Abstract
Ambrosia artemisiifolia is a well-known invasive species in Europe, causing health issues with its extremely allergenic pollen and yield loss through competition in agriculture. One potential biological control agent is Ophraella communa, accidentally introduced in Europe in 2013. This species was discovered [...] Read more.
Ambrosia artemisiifolia is a well-known invasive species in Europe, causing health issues with its extremely allergenic pollen and yield loss through competition in agriculture. One potential biological control agent is Ophraella communa, accidentally introduced in Europe in 2013. This species was discovered in France in 2023, but before planning further mass releases for biological control, it is necessary to assess its safety for agricultural crops and local plant biodiversity. Prior to its first detection in France, we conducted a host-range study of the beetle in a confined laboratory with no-choice and choice tests for 16 plant species, selected based on the centrifugal phylogenetic method. Results showed a restricted host range to the Heliantheae tribe and minimal risk to indigenous European plant species, with no larval survival and barely any eggs laid on these plants. Choice tests also showed a strong preference of O. communa for A. artemisiifolia. Our results combined with previous studies and observations in the field in other countries suggest a low risk to sunflower, Helianthus annuus, which is an important crop in France. This confirms that O. communa could be a low-risk biological control agent that can be used in classical biological control programs against A. artemisiifolia in France. Full article
(This article belongs to the Special Issue Embracing Systems Thinking in Crop Protection Science)
Show Figures

Figure 1

13 pages, 1181 KiB  
Review
Trade-Off Regulation in Plant Growth and Stress Responses Through the Role of Heterotrimeric G Protein Signaling
by Horim Lee
Plants 2024, 13(22), 3239; https://doi.org/10.3390/plants13223239 - 19 Nov 2024
Viewed by 348
Abstract
Unlike animals, plants are sessile organisms that cannot migrate to more favorable conditions and must constantly adapt to a variety of biotic and abiotic stresses. Therefore, plants exhibit developmental plasticity to cope, which is probably based on the underlying trade-off mechanism that allocates [...] Read more.
Unlike animals, plants are sessile organisms that cannot migrate to more favorable conditions and must constantly adapt to a variety of biotic and abiotic stresses. Therefore, plants exhibit developmental plasticity to cope, which is probably based on the underlying trade-off mechanism that allocates energy expenditure between growth and stress responses to achieve appropriate growth and development under different environmental conditions. Plant heterotrimeric G protein signaling plays a crucial role in the trade-off involved in the regulation of normal growth and stress adaptation. This review examines the composition and signaling processes of heterotrimeric G proteins in plants, detailing how they balance growth and adaptive responses in plant immunity and thermomorphogenesis through recent advances in the field. Understanding the trade-offs associated with plant G protein signaling will have significant implications for agricultural innovation, particularly in the development of crops with improved resilience and minimal growth penalties under environmental stress. Full article
Show Figures

Figure 1

17 pages, 9077 KiB  
Article
Diversity and Virulence of Diaporthe Species Associated with Peach Trunk Diseases in China
by Ying Zhou, Wei Zhang, Pranami D. Abeywickrama, Zhizheng He, Zhixiang Zhang, Yonghua Li, Shifang Li, Zaifeng Fan and Jiye Yan
Plants 2024, 13(22), 3238; https://doi.org/10.3390/plants13223238 - 18 Nov 2024
Viewed by 546
Abstract
Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about [...] Read more.
Peach (Prunus persica L.) is one of the most important and oldest stone fruits grown in China. Though Diaporthe species have more commonly been reported as plant pathogens, endophytes and saprophytes with a wide range of plant hosts, little is known about the Diaporthe species associated with peach trunk diseases in China. In the present study, forty-four Diaporthe isolates were obtained from trees with peach branch canker, shoot blight and gummosis symptoms in four provinces in China. Based on a combination of morphology and multi-locus sequence analysis of the rDNA internal transcribed spacer region (ITS), calmodulin (cal), translation elongation factor 1-α (tef1) and β-tubulin (tub2), these Diaporthe isolates were assigned to four species. Detailed descriptions and illustrations of all of the species, D. arecae, D. caulivora, D. discoidispora and D. eres, are provided. This study further reports the first host association of D. caulivora and D. discoidispora on peaches worldwide. The pathogenicity experiment results revealed that D. arecae was the most aggressive species, whereas D. discoidispora was the least aggressive on detached peach shoots. This study provides new insights into the fungi associated with peach trunk diseases in China, and the results of this study may help to facilitate routine diagnosis and planning of suitable plant disease management strategies. Full article
(This article belongs to the Special Issue Mycology and Plant Pathology)
Show Figures

Figure 1

22 pages, 1028 KiB  
Review
Genetic Basis of Tillering Angle from Other Plants to Wheat: Current Progress and Future Perspectives
by Xiaohong Chen, Tingshu Lei, Yuming Yan, Mengyu Sun, Tao Zhong, Baolin Wu, Hanxi Liu, Chao Zhang, Fengli Sun and Yajun Xi
Plants 2024, 13(22), 3237; https://doi.org/10.3390/plants13223237 - 18 Nov 2024
Viewed by 523
Abstract
Plant architecture is an important agronomic trait that impacts crop yield. The tiller angle is a critical aspect of the plant’s structural organization, which is influenced by both internal and external factors. The genetic mechanisms underlying the tiller angle have been extensively investigated [...] Read more.
Plant architecture is an important agronomic trait that impacts crop yield. The tiller angle is a critical aspect of the plant’s structural organization, which is influenced by both internal and external factors. The genetic mechanisms underlying the tiller angle have been extensively investigated in other plants. However, research on wheat is relatively limited. Additionally, mechanics has emerged as a connection between biochemical signaling and the development of three-dimensional biological forms. It not only reveals how physical interactions at the cellular level influence overall morphogenesis but also elucidates the interplay between these mechanical processes and molecular signaling pathways that collectively determine plant morphology. This review examines the recent advancements in the study of tillering angle in wheat and other plants. It discusses progress in research ranging from observable characteristics to the regulation of genes, as well as the physiological and biochemical aspects, and the adaptability to environmental factors. In addition, this review also discusses the effects of mechanical on plant growth and development, and provides ideas for the study of mechanical regulation mechanism of tillering angle in wheat. Consequently, based on the research of other plants and combined with the genetic and mechanical principles, this approach offers novel insights and methodologies for studying tillering in wheat. This interdisciplinary research framework not only enhances our understanding of the mechanisms underlying wheat growth and development but may also uncover the critical factors that regulate tillering angle, thereby providing a scientific foundation for improving wheat yield and adaptability. Full article
Show Figures

Figure 1

13 pages, 3504 KiB  
Article
Establishment of Dittrichia viscosa L. Hairy Roots and Improvement of Bioactive Compound Production
by Annalisa Paradiso, Miriana Durante, Sofia Caretto and Angelo De Paolis
Plants 2024, 13(22), 3236; https://doi.org/10.3390/plants13223236 - 18 Nov 2024
Viewed by 421
Abstract
Dittrichia viscosa is a ruderal plant species growing along roadsides and well adapting to extreme environmental conditions. D. viscosa plant tissues, especially leaves, are known to be a rich source of bioactive metabolites which have antioxidant, cytotoxic, antiproliferative and anticancer properties. Hairy root [...] Read more.
Dittrichia viscosa is a ruderal plant species growing along roadsides and well adapting to extreme environmental conditions. D. viscosa plant tissues, especially leaves, are known to be a rich source of bioactive metabolites which have antioxidant, cytotoxic, antiproliferative and anticancer properties. Hairy root cultures are a suitable biotechnological system for investigating plant metabolic pathways and producing specialized metabolites in in vitro conditions. In this study, D. viscosa hairy root transformed lines induced by Agrobacterium rhizogenes ATCC15834 were obtained using leaf explants, and the integration of rolB and rolC genes in the genomes of transformed hairy roots were confirmed by PCR analysis. Three hairy root D. viscosa lines (DvHrT1, DvHrT4 and DvHrT5) having different phenotypic features were characterized in terms of total phenolics, flavonoids and antioxidant activity. Correlated with antioxidant activity, phenolic and flavonoid content of DvHrT1 was significantly higher than control roots and the other DvHrT lines. Our results suggest that D. viscosa hairy roots can be a valuable tool for producing various bioactive compounds having antioxidant activity and are to be further investigated to produce other specific molecules that could find application in agricultural or pharmaceutical fields. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
Show Figures

Figure 1

14 pages, 2798 KiB  
Article
Ethnopharmacological Study of Garrya laurifolia and Its Antidiabetic Effect in Rats
by María Mirian Estévez-Carmona, Saudy Saret Pablo-Pérez, Jesús Eduardo Almanza-Cruz, María Estela Meléndez-Camargo, Daniel Arrieta-Baez, José Melesio Cristóbal-Luna and Margarita Franco-Colín
Plants 2024, 13(22), 3235; https://doi.org/10.3390/plants13223235 - 18 Nov 2024
Viewed by 416
Abstract
This study aimed to scientifically validate the traditional use of Garrya laurifolia (Gl) leaves as an antidiabetic agent attributed to a community in Mexico. The descriptive ethnobotanical study was conducted in San Miguel Tecpan, a municipality of Jilotzingo, State of Mexico, Mexico, where [...] Read more.
This study aimed to scientifically validate the traditional use of Garrya laurifolia (Gl) leaves as an antidiabetic agent attributed to a community in Mexico. The descriptive ethnobotanical study was conducted in San Miguel Tecpan, a municipality of Jilotzingo, State of Mexico, Mexico, where a structured questionnaire was applied to 44 inhabitants. In vivo studies evaluated the acute oral toxicity of Gl leaves in murine and the effects of a leaf infusion on glycemia in normoglycemic and diabetic rats; in addition, the interaction between Gl and metformin (Met) was also evaluated. The in vitro antioxidant activity of Gl was determined. The phytochemical screening and quantification of phenolic and flavonoid content of Gl leaves were performed. Gl had a high relative frequency of citation (0.68) among respondents. Gl had a low acute toxicity risk with LD50 > 5000 mg/kg. The extract had no hypoglycemic effect in normoglycemic rats, but it did have hypoglycemic and antihyperglycemic effects (250 and 500 mg/kg) in diabetic rats. The interaction between Gl (500 mg/kg) + Met (300 mg/kg) resulted in antidiabetic synergism. Gl showed strong antioxidant activity (93.1 ± 0.4%). Phytochemical screening revealed the presence of alkaloids, flavonoids, and some other phenolic compounds. The total phenol content was 77.9 ± 0.6 mg EQ/g and 87.7 ± 0.7 mg EAG/g, and the flavonoids content was 5.32 ± 0.2 mg EQ/g. UHPLC-MS/MS analysis identified chlorogenic acid, rutin, aucubin, luteolin 7-O-neohesperoside, and myricitrin. The findings support the potential use of Gl as a safe and effective antidiabetic agent. Full article
(This article belongs to the Special Issue Ethnobotanical Studies of Medicinal Plants)
Show Figures

Figure 1

18 pages, 19571 KiB  
Article
Impacts of Climate Changes on Spatiotemporal Variation of Cotton Water Requirement and Irrigation in Tarim Basin, Central Asia
by Min Xu, Hao Wu and Xiaoping Chen
Plants 2024, 13(22), 3234; https://doi.org/10.3390/plants13223234 - 18 Nov 2024
Viewed by 433
Abstract
Evapotranspiration (ETc), crop water requirement (Dcr), irrigation (IR) and irrigation leaching amount (IRlc) play a critical role in optimizing irrigation scheduling and are also important for hydrological cycle processes and ecological environment [...] Read more.
Evapotranspiration (ETc), crop water requirement (Dcr), irrigation (IR) and irrigation leaching amount (IRlc) play a critical role in optimizing irrigation scheduling and are also important for hydrological cycle processes and ecological environment in arid regions. This research examined the spatiotemporal variability of the ETc, Dcr and IR of cotton using data from 16 meteorological stations in the Tarim basin (TRB) of arid Northwest China during 1961–2017. The results showed that the mean annual ETc of cotton exhibited a significant decreasing trend, with a change rate of 12.965 mm·10 a−1 and 18.357 mm·10 a−1 during 1961–2017 and 1961–1990, respectively. Subsequently, it experienced a substantial increase with a change rate of 16.833 mm·10 a−1 after 1990. The Dcr of cotton followed a decreasing trend at a rate of 15.531 mm·10 a−1 and 21.99 mm·10 a−1 during 1961–2017 and 1961–1990, respectively. The Dcr of cotton provided an increasing trend at a rate of 20.164 mm·10 a−1 during 1991–2017. The IR of cotton followed a decreasing trend at a rate of 19.66 mm·10 a−1 in 1961–2017 and 24.531 mm·10 a−1 in 1961–1990, but an increasing trend at a rate of 14.437 mm·10 a−1 in 1991–2017. The IRlc of cotton decreased by 2.566 mm·10 a−1 and 3.663 mm·10 a−1 during 1961–2017 and 1961–1990, respectively. After 1990, it experienced a substantial increase by 3.331 mm·10 a−1. Wind speed exerted the greatest influence on the variability in Dcr and IR between 1961 and 1990, while shine hour played a more prominent role in explaining the variability in Dcr and precipitation may have played a more significant role in explaining the variability in IR. This study is helpful for the scientific planning for agriculture, water resource allocation and water-saving irrigation in arid regions. Full article
(This article belongs to the Special Issue Agricultural Water Pollution Treatment and Water Use Safety)
Show Figures

Figure 1

27 pages, 6374 KiB  
Review
Beyond Essential Oils: Diterpenes, Lignans, and Biflavonoids from Juniperus communis L. as a Source of Multi-Target Lead Compounds
by Alina Arabela Jojić, Sergio Liga, Diana Uţu, Graţiana Ruse, Liana Suciu, Andrei Motoc, Codruța Marinela Şoica and Diana-Simona Tchiakpe-Antal
Plants 2024, 13(22), 3233; https://doi.org/10.3390/plants13223233 - 17 Nov 2024
Viewed by 498
Abstract
Common Juniper (Juniperus communis L.) is a gymnosperm that stands out through its fleshy, spherical female cones, often termed simply “berries”. The cone berries and various vegetative parts (leaves, twigs and even roots) are used in traditional phytotherapy, based on the beneficial [...] Read more.
Common Juniper (Juniperus communis L.) is a gymnosperm that stands out through its fleshy, spherical female cones, often termed simply “berries”. The cone berries and various vegetative parts (leaves, twigs and even roots) are used in traditional phytotherapy, based on the beneficial effects exerted by a variety of secondary metabolites. While the volatile compounds of Juniperus communis are known for their aromatic properties and have been well-researched for their antimicrobial effects, this review shifts focus to non-volatile secondary metabolites—specifically diterpenes, lignans, and biflavonoids. These compounds are of significant biomedical interest due to their notable pharmacological activities, including antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. The aim of this review is to offer an up-to-date account of chemical composition of Juniperus communis and related species, with a primary emphasis on the bioactivities of diterpenes, lignans, and biflavonoids. By examining recent preclinical and clinical data, this work assesses the therapeutic potential of these metabolites and their mechanisms of action, underscoring their value in developing new therapeutic options. Additionally, this review addresses the pharmacological efficacy and possible therapeutic applications of Juniperus communis in treating various human diseases, thus supporting its potential role in evidence-based phytotherapy. Full article
(This article belongs to the Section Phytochemistry)
Show Figures

Figure 1

21 pages, 3964 KiB  
Article
Emission and Transcriptional Regulation of Aroma Variation in Oncidium Twinkle ‘Red Fantasy’ Under Diel Rhythm
by Yan Chen, Shengyuan Zhong, Lan Kong, Ronghui Fan, Yan Xu, Yiquan Chen and Huaiqin Zhong
Plants 2024, 13(22), 3232; https://doi.org/10.3390/plants13223232 - 17 Nov 2024
Viewed by 316
Abstract
Oncidium hybridum is one of the important cut-flowers in the world. However, the lack of aroma in its cut-flower varieties greatly limits the sustainable development of the Oncidium hybridum cut-flowers industry. This paper is an integral investigation of the diel pattern and influencing [...] Read more.
Oncidium hybridum is one of the important cut-flowers in the world. However, the lack of aroma in its cut-flower varieties greatly limits the sustainable development of the Oncidium hybridum cut-flowers industry. This paper is an integral investigation of the diel pattern and influencing factors of the aroma release of Oncidium Twinkle ‘Red Fantasy’. GC-MS analysis revealed that the release of 3-Carene peaked at 10:00, while Butyl tiglate and Prenyl senecioate did so at 14:00, with a diel rhythm. By analyzing the correlation network between aroma component synthesis and differentially expressed genes, 15 key structural genes were detected and regulated by multiple circadian rhythm-related transcription factors. Cluster-17371.18_TPS, Cluster-65495.1_TPS, Cluster-46699.0_TPS, Cluster-60935.10_DXS, Cluster-47205.4_IDI, and Cluster-65313.7_LOX were key genes in the terpenoid and fatty acid derivative biosynthetic pathway, which were co-expressed with aroma release. Constant light/dark treatments revealed that the diurnal release of 3-Carene may be influenced by light and the circadian clock, and Butyl tiglate and Prenyl senecioate may be mainly determined by endogenous circadian clock. Under constant light treatment, the TPS, DXS, IDI, and LOX genes seem to lose their regulatory role in the release of aroma compounds from Oncidium Twinkle ‘Red Fantasy’. Under constant dark treatment, the TPS genes were consistent with the release pattern of 3-Carene, which may be a key factor in regulating the diel rhythm of 3-Carene biosynthesis. These results laid a theoretical foundation for the study of floral transcriptional regulation and genetic engineering technology breeding of Oncidium hybridum. Full article
(This article belongs to the Special Issue Recent Advances in Horticultural Plant Genomics)
Show Figures

Figure 1

14 pages, 1092 KiB  
Article
GC/MS Analysis and Protective Effects of Mentha longifolia L. Essential Oil Against Antituberculosis Drug-Induced Organs Toxicity in Wistar Albino Rats
by Usama K. Abdel-Hameed, Abdulaziz S. Abualghaith, Shaza H. Aly, Mohamed Mostafa Soliman, Lamiaa Adnan Munshi, Safia A. A. Mohammed, Omayma A. Eldahshan and Eman A. R. Abdelghffar
Plants 2024, 13(22), 3231; https://doi.org/10.3390/plants13223231 - 17 Nov 2024
Viewed by 454
Abstract
Mentha longifolia (L.) L., also known as wild mint, is a perennial herbaceous plant that belongs to the Lamiaceae family. This study aimed to investigate the effects of essential oil of M. longifolia (MLEO) on oxidative stress and inflammatory responses in the liver [...] Read more.
Mentha longifolia (L.) L., also known as wild mint, is a perennial herbaceous plant that belongs to the Lamiaceae family. This study aimed to investigate the effects of essential oil of M. longifolia (MLEO) on oxidative stress and inflammatory responses in the liver and kidneys in the context of drug-induced liver injury caused by the anti-TB drugs rifampicin, isoniazid, and pyrazinamide (INH-RIF-PZA). The chemical composition of MLEO was characterized using GC/MS analysis, which revealed the presence of pulegone, trans-p-menthan-3-one, piperitenone, and β-caryophyllene as its major volatile constituents. An INH/RIF/PZA mixture was administered to Wistar rats for 30 days, and silymarin was administered as a standard drug. MLEO was administered p.o. at doses of 50 mg and 100 mg/kg b.w. Both doses of the MLEO therapy effectively regulated all biochemical indicators of hepatic impairment and reduced the damage caused by the INH/RIF/PZA mixture. It may be deduced that MLEO has the ability to protect organs against INH/RIF/PZA-induced damage and could potentially be a valuable natural remedy for treating anti-TB-induced liver and kidney injuries. Full article
(This article belongs to the Special Issue Chemical Composition and Biological Activities of Essential Oils)
Show Figures

Figure 1

Previous Issue
Back to TopTop