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Advances in Electrochemotherapy
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Advances in Electrochemotherapy

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 20 March 2025 | Viewed by 2024

Special Issue Editors

Prof. Dr. Jurij Novickij

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Guest Editor
Institute of Telecommunication, Vilnius Gediminas Technical University, Vilnius, Lithuania
Interests: nanosecond electrochemotherapy
Dr. Julita Kulbacka

E-Mail Website
Guest Editor
Department of Molecular and Cellular Biology, Wroclaw Medical University, 50-367 Wroclaw, Poland
Interests: drug delivery; drug resistance; electroporation; photodynamic therapy; oxidative stress and free radicals; natural chemotherapeutics; nanotechnology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Maja Čemažar

E-Mail Website
Guest Editor
National Research Council—Institute of Translational Pharmacology (CNR-IFT), Via Fosso del Cavaliere 100, Rome, Italy
Interests: gene electrotransfer; cancer gene therapy; electroporation; plasmid DNA; electrochemotherapy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Electrochemotherapy (ECT) is a safe and efficient procedure for the treatment of cancer, relying on the transient permeability of the cell plasma membrane (i.e., electroporation), which facilitates targeted drug delivery. As a result, ECT ensures the localized treatment of tumors, making it a popular procedure in clinics and a safer alternative to conventional treatments. However, a deeper insight into electrochemotherapy at the molecular level is needed, including into the possibility of modulating the immune response to secure a more effective treatment process, even in the case of metastases. Additionally, novel parametric protocols and accompanying molecular mechanisms should be continually researched to counter the multi-drug resistance (MDR) of cancer and to promote the further evolution of its treatment. Therefore, this Special Issue focuses on advances in and applications of ECT.

Prof. Dr. Jurij Novickij
Prof. Dr. Maja Čemažar
Prof. Dr. Julita Kulbacka
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemotherapy
  • drug delivery
  • pulsed electric fields
  • cancer
  • drug resistance

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Published Papers (2 papers)

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Research

12 pages, 2543 KiB  
Article
The Effects of Bipolar Cancellation Phenomenon on Nano-Electrochemotherapy of Melanoma Tumors: In Vitro and In Vivo Pilot
by Eglė Mickevičiūtė, Eivina Radzevičiūtė-Valčiukė, Veronika Malyško-Ptašinskė, Paulina Malakauskaitė, Barbora Lekešytė, Nina Rembialkowska, Julita Kulbacka, Joanna Tunikowska, Jurij Novickij and Vitalij Novickij
Int. J. Mol. Sci. 2024, 25(17), 9338; https://doi.org/10.3390/ijms25179338 - 28 Aug 2024
Viewed by 767
Abstract
The phenomenon known as bipolar cancellation is observed when biphasic nanosecond electric field pulses are used, which results in reduced electroporation efficiency when compared to unipolar pulses of the same parameters. Basically, the negative phase of the bipolar pulse diminishes the effect of [...] Read more.
The phenomenon known as bipolar cancellation is observed when biphasic nanosecond electric field pulses are used, which results in reduced electroporation efficiency when compared to unipolar pulses of the same parameters. Basically, the negative phase of the bipolar pulse diminishes the effect of the positive phase. Our study aimed to investigate how bipolar cancellation affects Ca2+ electrochemotherapy and cellular response under varying electric field intensities and pulse durations (3–7 kV/cm, 100, 300, and 500 ns bipolar 1 MHz repetition frequency pulse bursts, n = 100). As a reference, standard microsecond range parametric protocols were used (100 µs × 8 pulses). We have shown that the cancellation effect is extremely strong when the pulses are closely spaced (1 MHz frequency), which results in a lack of cell membrane permeabilization and consequent failure of electrochemotherapy in vitro. To validate the observations, we have performed a pilot in vivo study where we compared the efficacy of monophasic (5 kV/cm × ↑500 ns × 100) and biphasic sequences (5 kV/cm × ↑500 ns + ↓500 ns × 100) delivered at 1 MHz frequency in the context of Ca2+ electrochemotherapy (B16-F10 cell line, C57BL/6 mice, n = 24). Mice treated with bipolar pulses did not exhibit prolonged survival when compared to the untreated control (tumor-bearing mice); therefore, the bipolar cancellation phenomenon was also occurrent in vivo, significantly impairing electrochemotherapy. At the same time, the efficacy of monophasic nanosecond pulses was comparable to 1.4 kV/cm × 100 µs × 8 pulses sequence, resulting in tumor reduction following the treatment and prolonged survival of the animals. Full article
(This article belongs to the Special Issue Advances in Electrochemotherapy)
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15 pages, 6094 KiB  
Article
Threshold Interphase Delay for Bipolar Pulses to Prevent Cancellation Phenomenon during Electrochemotherapy
by Veronika Malyško-Ptašinskė, Aušra Nemeikaitė-Čėnienė, Eivina Radzevičiūtė-Valčiukė, Eglė Mickevičiūtė, Paulina Malakauskaitė, Barbora Lekešytė and Vitalij Novickij
Int. J. Mol. Sci. 2024, 25(16), 8774; https://doi.org/10.3390/ijms25168774 - 12 Aug 2024
Viewed by 712
Abstract
Electroporation-based procedures employing nanosecond bipolar pulses are commonly linked to an undesirable phenomenon known as the cancelation effect. The cancellation effect arises when the second pulse partially or completely neutralizes the effects of the first pulse, simultaneously diminishing cells’ plasma membrane permeabilization and [...] Read more.
Electroporation-based procedures employing nanosecond bipolar pulses are commonly linked to an undesirable phenomenon known as the cancelation effect. The cancellation effect arises when the second pulse partially or completely neutralizes the effects of the first pulse, simultaneously diminishing cells’ plasma membrane permeabilization and the overall efficiency of the procedure. Introducing a temporal gap between the positive and negative phases of the bipolar pulses during electroporation procedures may help to overcome the cancellation phenomenon; however, the exact thresholds are not yet known. Therefore, in this work, we have tested the influence of different interphase delay values (from 0 ms to 95 ms) using symmetric bipolar nanoseconds (300 and 500 ns) on cell permeabilization using 10 Hz, 100 Hz, and 1 kHz protocols. As a model mouse hepatoma, the MH-22a cell line was employed. Additionally, we conducted in vitro electrochemotherapy with cisplatin, employing reduced interphase delay values (0 ms and 0.1 ms) at 10 Hz. Cell plasma membrane permeabilization and viability dependence on a variety of bipolar pulsed electric field protocols were characterized. It was shown that it is possible to minimize bipolar cancellation, enabling treatment efficiency comparable to monophasic pulses with identical parameters. At the same time, it was highlighted that bipolar cancellation has a significant influence on permeabilization, while the effects on the outcome of electrochemotherapy are minimal. Full article
(This article belongs to the Special Issue Advances in Electrochemotherapy)
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