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Inflammatory Bowel Diseases: Molecular Mechanism and Therapeutics

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 20 June 2025 | Viewed by 922

Special Issue Editor


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Guest Editor
Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University College of Medicine, Incheon 22212, Republic of Korea
Interests: animal model; mini-pig; efficacy evaluation; disease; preclinical study; inflammatory bowel disease
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Special Issue Information

Dear Colleagues,

Inflammatory bowel disease (IBD) is an inflammatory disease of the gastrointestinal tract that includes ulcerative colitis (UC) and Crohn’s disease (CD) and has the potential to develop into colon cancer over the long term. The purpose of this Special Issue is to explore the molecular mechanisms and treatments of IBD to gain a deeper understanding of the pathophysiology of IBD and new therapeutic approaches to treat these diseases. Various animal models have played a key role in the study of inflammatory bowel disease. In particular, mini pigs have become valuable animal models in biomedical research because of their physiological and anatomical similarities to humans. Animal models are helpful for studying the molecular progression of the disease and evaluating treatments, as well as future research areas including drug development.

This Special Issue welcomes original papers and reviews related to the molecular mechanisms, pathophysiology, and treatment of IBD. IJMS is a journal of molecular science; therefore, pure clinical or model studies will not be suitable for our journal. But clinical or pure model submissions with biomolecular experiments are welcomed.

Dr. Woon Kyu Lee
Guest Editor

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Keywords

  • inflammatory bowel disease
  • IBD
  • ulcerative colitis
  • Crohn’s disease
  • animal models

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Published Papers (1 paper)

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Research

22 pages, 2203 KiB  
Article
Proteomic Profiling of Extracellular Vesicles in Inflammatory Bowel Diseases
by Montse Baldán-Martín, Mikel Azkargorta, Ainhoa Lapitz, Lorena Ortega Moreno, Ibon Iloro, Samuel Fernández-Tomé, Ander Arbelaiz, Iraide Escobes, Alicia C. Marín, David Bernardo, Luis Bujanda, Jesús M. Bañales, Felix Elortza, Javier P. Gisbert and María Chaparro
Int. J. Mol. Sci. 2025, 26(2), 526; https://doi.org/10.3390/ijms26020526 - 9 Jan 2025
Viewed by 501
Abstract
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD [...] Read more.
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group). All groups were matched for age and sex. Disease activity was assessed by ileocolonoscopy and categorized based on the SES-CD (CD) and the endoscopic sub-score of the Mayo Score (UC). EVs were enriched by ul-tracentrifugation, and their size and concentration were determined by nanoparticle tracking analysis. The expression of CD63, CD81, and CD9 was determined using West-ern blotting. Proteomic analysis was performed by label-free nano-LC MS/MS. A total of 324 proteins were identified; 60 showed differential abundance in CD-HC, 34 in UC-HC, and 21 in CD-UC. Regarding disease activity, the abundance of 58 and 32 proteins was altered in aCD-HC and aUC-HC, respectively. Functional analyses revealed that proteins associated with aCD were involved in immune regulation, whereas those linked to aUC were enriched in oxidative stress. We have identified expressed proteins between EVs from patients with CD and UC, depending on the presence of disease, the disease type, and the disease activity. These proteins are potential candidates as disease biomarkers and open new research avenues to better understand these conditions. Full article
(This article belongs to the Special Issue Inflammatory Bowel Diseases: Molecular Mechanism and Therapeutics)
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