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Synthetic Biology Research Based on a Yarrowia lipolytica Model

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 December 2024 | Viewed by 2059

Special Issue Editor


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Guest Editor
INRA INA-PG CNRS, Centre de Biotechnologie Agro-Industrielle, F-78850 Thiverval-Grignon, France
Interests: lipid accumulation; microbial genetics; metabolic engineering; biotechnology of yeasts; algal engineering
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Special Issue Information

Dear Colleagues, 

Yarrowia lipolytica has gained significant interest in synthetic biology research due to its unique characteristics and metabolic capabilities. Y. lipolytica has been extensively used as a model organism in synthetic biology due to its ability to utilize a wide range of carbon sources, including various hydrophobic substrates. Researchers have used metabolic engineering techniques to enhance its lipid-accumulation capabilities to produce biofuels, oleochemicals, and other high-value lipids. Synthetic biology approaches have been employed to enhance lipid production in Y. lipolytica via engineering metabolic pathways involved in lipid synthesis and regulation. Researchers have engineered Y. lipolytica strains to produce various proteins, including therapeutic enzymes, organic acids, polymers, and pharmaceutical precursors. Its secretion machinery and post-translational modification capabilities make it suitable to produce complex proteins. Synthetic biology techniques have enabled the engineering of Y. lipolytica strains to produce enzymes with desired properties, such as thermostability, pH tolerance, or substrate specificity. Researchers have engineered Y. lipolytica strains that can be controlled or triggered by light, enabling the precise modulation of gene expression and metabolic activities. These applications make Y. lipolytica a promising platform that serves as a valuable model organism in synthetic biology for developing sustainable biotechnological solutions.

This Special Issue calls for articles and reviews related to synthetic biology research on Yarrowia lipolytica.

Dr. Jean-Marc Nicaud
Guest Editor

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Keywords

  • Y. lipolytica
  • synthetic biology
  • metabolic engineering
  • lipid accumulation
  • oleochemistry
  • biofuels
  • sustainable biotechnology

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Published Papers (1 paper)

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14 pages, 2530 KiB  
Article
Revealing the Mechanisms of Enhanced β-Farnesene Production in Yarrowia lipolytica through Metabolomics Analysis
by Qianxi Liu, Haoran Bi, Kai Wang, Yang Zhang, Biqiang Chen, Huili Zhang, Meng Wang and Yunming Fang
Int. J. Mol. Sci. 2023, 24(24), 17366; https://doi.org/10.3390/ijms242417366 - 11 Dec 2023
Cited by 2 | Viewed by 1659
Abstract
β-Farnesene is an advanced molecule with promising applications in agriculture, the cosmetics industry, pharmaceuticals, and bioenergy. To supplement the shortcomings of rational design in the development of high-producing β-farnesene strains, a Metabolic Pathway Design-Fermentation Test-Metabolomic Analysis-Target Mining experimental cycle was designed. In this [...] Read more.
β-Farnesene is an advanced molecule with promising applications in agriculture, the cosmetics industry, pharmaceuticals, and bioenergy. To supplement the shortcomings of rational design in the development of high-producing β-farnesene strains, a Metabolic Pathway Design-Fermentation Test-Metabolomic Analysis-Target Mining experimental cycle was designed. In this study, by over-adding 20 different amino acids/nucleobases to induce fluctuations in the production of β-farnesene, the changes in intracellular metabolites in the β-farnesene titer-increased group were analyzed using non-targeted metabolomics. Differential metabolites that were detected in each experimental group were selected, and their metabolic pathways were located. Based on these differential metabolites, targeted strain gene editing and culture medium optimization were performed. The overexpression of the coenzyme A synthesis-related gene pantothenate kinase (PanK) and the addition of four mixed water-soluble vitamins in the culture medium increased the β-farnesene titer in the shake flask to 1054.8 mg/L, a 48.5% increase from the initial strain. In the subsequent fed-batch fermentation, the β-farnesene titer further reached 24.6 g/L. This work demonstrates the tremendous application value of metabolomics analysis for the development of industrial recombinant strains and the optimization of fermentation conditions. Full article
(This article belongs to the Special Issue Synthetic Biology Research Based on a Yarrowia lipolytica Model)
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