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Research on Advanced Bioanalytical Approaches for Biomolecules Detection

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Informatics".

Deadline for manuscript submissions: 20 January 2025 | Viewed by 1377

Special Issue Editors


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Guest Editor
1. NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, LT-03225 Vilnius, Lithuania
2. Department of Immunology and Bioelectrochemistry, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania
Interests: biosensors; immunosensors for bioanalytical and biomedical application; electrochemical, optical and piezoelectric signal transducers; nanoparticles and nanostructured surfaces; synthesis and application of conducting polymers; immobilization of biomolecules
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
1. NanoTechnas—Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, LT-03225 Vilnius, Lithuania
2. Department of Immunology and Bioelectrochemistry, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania
Interests: immunosensors; biosensors; the use of nanomaterials for the fabrication of sensors; conducting polymers
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
1. NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, LT-03225 Vilnius, Lithuania
2. Department of Immunology and Bioelectrochemistry, State Research Institute Centre for Innovative Medicine, LT-08406 Vilnius, Lithuania
Interests: electrochemical enzymatic biosensors and immunosensors; optical immunosensors; enzymatic biofuel cells and self-powered biosensors; synthesis and application of nanomaterials for biosensing

Special Issue Information

Dear Colleagues,

Bioanalytical chemistry commonly focuses on advanced techniques and innovative methods and their application in solving medical and clinical, food safety, and environmental control issues. There is an urgent need for rapid, simple, and ultrasensitive detection of molecules by miniaturized and affordable immunoanalytical and bioanalytical systems. Timely detection of various molecules in different matrices can prevent the spread of infectious and non-infectious diseases, ensure food safety and quality, and protect the population from harmful environmental effects. Recent advances in nanotechnology, the application of molecularly imprinted polymers in affinity sensors, the miniaturization of bioanalytical systems, and the possibility of multiplex detection of several molecules in a single measurement open up new opportunities compared to the conventional currently used immunoanalytical and bioanalytical methods. In addition, conventional diagnostic methods have limited applications in low-resource settings due to the use of bulky and expensive instrumentation.

This Special Issue of IJMS is focused on the recent progress and cutting-edge solutions applied in immunoanalytical and bioanalytical systems, including biosensors, immunosensors, and affinity sensors. Original research papers, communications, and review articles are welcome.

Prof. Dr. Almira Ramanaviciene
Dr. Anton Popov
Dr. Asta Kausaite-Minkstimiene
Guest Editors

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Keywords

  • biosensors
  • immunosensors
  • immuno- or bio-assay
  • molecularly imprinted polymers
  • nanoparticles
  • biomarkers
  • analytical signal amplification strategies

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Published Papers (1 paper)

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Research

13 pages, 1653 KiB  
Article
Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein
by Viktorija Lisyte, Asta Kausaite-Minkstimiene, Benediktas Brasiunas, Anton Popov and Almira Ramanaviciene
Int. J. Mol. Sci. 2024, 25(16), 8574; https://doi.org/10.3390/ijms25168574 - 6 Aug 2024
Viewed by 966
Abstract
The strong immunogenicity of the SARS-CoV-2 nucleocapsid protein is widely recognized, and the detection of specific antibodies is critical for COVID-19 diagnostics in patients. This research proposed direct, label-free, and sensitive detection of antibodies against the SARS-CoV-2 nucleocapsid protein (anti-SCoV2-rN). Recombinant SARS-CoV-2 nucleocapsid [...] Read more.
The strong immunogenicity of the SARS-CoV-2 nucleocapsid protein is widely recognized, and the detection of specific antibodies is critical for COVID-19 diagnostics in patients. This research proposed direct, label-free, and sensitive detection of antibodies against the SARS-CoV-2 nucleocapsid protein (anti-SCoV2-rN). Recombinant SARS-CoV-2 nucleocapsid protein (SCoV2-rN) was immobilized by carbodiimide chemistry on an SPR sensor chip coated with a self-assembled monolayer of 11-mercaptoundecanoic acid. When immobilized under optimal conditions, a SCoV2-rN surface mass concentration of 3.61 ± 0.52 ng/mm2 was achieved, maximizing the effectiveness of the immunosensor for the anti-SCoV2-rN determination. The calculated KD value of 6.49 × 10−8 ± 5.3 × 10−9 M confirmed the good affinity of the used monoclonal anti-SCoV2-rN antibodies. The linear range of the developed immunosensor was from 0.5 to 50 nM of anti-SCoV2-rN, where the limit of detection and the limit of quantification values were 0.057 and 0.19 nM, respectively. The immunosensor exhibited good reproducibility and specificity. In addition, the developed immunosensor is suitable for multiple anti-SCoV2-rN antibody detections. Full article
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