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New Perspectives in Steroidomics

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Endocrinology and Metabolism".

Deadline for manuscript submissions: 30 January 2025 | Viewed by 739

Special Issue Editor


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Guest Editor
Department of Steroids and Proteofactors, Institute of Endocrinology, Národní 8, 116 94 Prague, Czech Republic
Interests: steroids; steroidomics; chemometrics; neuroactive steroids; steroids and pregnancy; chromatography
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Since the beginning of 2010, the term steroidomics can be found in the literature, but studies corresponding to this term were published long before that because steroidomics is a field that focuses on high-throughput profiling and quantification of steroids, most commonly by liquid or gas chromatography with tandem mass spectrometric detection. As maintaining balanced steroid levels is essential for overall health, while imbalances can contribute to disease, researchers are exploring steroidomics for applications in the prevention, evaluation, and treatment of various pathologies such as cancer, diabetes, neurodegenerative and neuropsychiatric disorders, pregnancy complications, various gonadal or adrenal disorders, which are often age-related, and others. This Special Issue, “New Perspectives in Steroidomics”, will focus on successful solutions related to the application of steroidomics not only in the areas mentioned above but particularly in areas where this approach has not yet been used. Studies utilizing steroidomics in the investigation of physiological processes will also be welcome.

Dr. Martin Hill
Guest Editor

Manuscript Submission Information

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Keywords

  • steroidomics
  • steroids
  • pathophysiology
  • physiology
  • diagnostics
  • treatment
  • prediction
  • prognosis
  • LC-MS/MS
  • GC-MS/MS

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Published Papers (1 paper)

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Research

37 pages, 2265 KiB  
Article
Altered Steroidome in Women with Multiple Sclerosis
by Radmila Kancheva, Martin Hill, Marta Velíková, Ludmila Kancheva, Josef Včelák, Radek Ampapa, Michal Židó, Ivana Štětkářová, Jana Libertínová, Michala Vosátková and Eva Kubala Havrdová
Int. J. Mol. Sci. 2024, 25(22), 12033; https://doi.org/10.3390/ijms252212033 - 8 Nov 2024
Viewed by 576
Abstract
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) mainly afflicting young women. Various steroids can influence the onset and development of the disease or, on the contrary, mitigate its course; however, a systematic review of steroidomic changes [...] Read more.
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) mainly afflicting young women. Various steroids can influence the onset and development of the disease or, on the contrary, mitigate its course; however, a systematic review of steroidomic changes in MS patients is lacking. Based on the gas chromatography tandem mass spectrometry (GC-MS/MS) platform and, in the case of estradiol, also using immunoassay, this study performed a comprehensive steroidomic analysis in 25 female MS patients aged 39(32, 49) years compared to 15 female age-matched controls aged 38(31, 46) years. A significant trend towards higher ratios of conjugated steroids to their unconjugated counterparts was found in patients, which is of particular interest in terms of the balance between excitatory and inhibitory steroid modulators of ionotropic receptors. Patients showed altered metabolic pathway to cortisol with decreased conversion of pregnenolone to 17-hydroxypregnenolone and 17-hydroxypregnenolone to 17-hydroxyprogesterone and increased conversion of 17-hydroxypregnenolone to dehydroepiandrosterone (DHEA), resulting in lower levels of 17-hydroxyprogesterone, as well as indications of impaired conversion of 11-deoxy-steroids to 11β-hydroxy-steroids but reduced conversion of cortisol to cortisone. Due to over-activation of hypothalamic-pituitary-adrenal axis (HPAA), however, cortisol and cortisone levels were higher in patients with indications of depleted cortisol synthesizing enzymes. Patients showed lower conversion of DHEA to androstenedione, androstenedione to testosterone, androstenedione to estradiol in the major pathway, and testosterone to estradiol in the minor pathway for estradiol synthesis at increased conversion of androstenedione to testosterone. They also showed lower conversion of immunoprotective Δ5 androstanes to their more potent 7α/β-hydroxy metabolites and had lower circulating allopregnanolone and higher ratio 3β-hydroxy-steroids to their neuroprotective 3α-hydroxy-counterparts. Full article
(This article belongs to the Special Issue New Perspectives in Steroidomics)
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