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Intracellular Organellar Ion Channels and Molecular Physiology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 5290

Special Issue Editors


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Guest Editor
Yale University, New Haven, CT, USA

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Guest Editor
CryoEM Center, Laboratory of Molecular Physiology and Biophysics, Hauptman-Woodward Medical Research Institute, SUNY-Buffalo. 700 Ellicott St., Buffalo, NY 14203-1102, USA
Interests: molecular physiology and biophysics; lipid-dependent gating of Kv channels; ion channels in regulated secretion; RNA-binding proteins; development of new technologies for membrane biology and cryoEM imaging
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Special Issue Information

Intracellular membranes have ion gradients across them. It is expected that ion channels and transporters play important roles in generating and/or maintaining these gradients and in using the electrochemical gradients to drive movement of signals and materials across membranes. In this Special Issue, experts will provide a detailed introduction to the ion channels of interest in each organelle and will discuss their physiological or pathophysiological functions. The intracellular membrane-enclosed organelles generally include ER, Golgi apparatus, mitochondria, lysosomes, chloroplasts, endosomes, peroxisomes, lysosome-related organelles, trafficking vesicles, secretory granules, synaptic vesicles, phagosomes, autophagosomes, vacuoles, etc. Exosomes are also considered because of their intracellular origin. The general theme is to discuss cation or anion channels discovered with relatively high certainty and crystallize the current understandings into a mechanistic basis for physiology and pathophysiology.

Prof. Elizabeth Jonas
Dr. Qiu-Xing Jiang
Guest Editors

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Published Papers (1 paper)

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Review

22 pages, 1860 KiB  
Review
Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment
by Agnieszka Walewska, Milena Krajewska, Aleksandra Stefanowska, Aleksandra Buta, Renata Bilewicz, Paweł Krysiński, Piotr Bednarczyk, Piotr Koprowski and Adam Szewczyk
Int. J. Mol. Sci. 2022, 23(3), 1210; https://doi.org/10.3390/ijms23031210 - 21 Jan 2022
Cited by 13 | Viewed by 4691
Abstract
In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we [...] Read more.
In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we focus on the critical evaluation of techniques used in the studies of mitochondrial potassium channels, describing their advantages and limitations. Functional analysis of mitochondrial potassium channels in comparison to that of plasmalemmal channels presents additional experimental challenges. The reliability of functional studies of mitochondrial potassium channels is often affected by the need to isolate mitochondria and by functional properties of mitochondria such as respiration, metabolic activity, swelling capacity, or high electrical potential. Three types of techniques are critically evaluated: electrophysiological techniques, potassium flux measurements, and biochemical techniques related to potassium flux measurements. Finally, new possible approaches to the study of the function of mitochondrial potassium channels are presented. We hope that this review will assist researchers in selecting reliable methods for studying, e.g., the effects of drugs on mitochondrial potassium channel function. Additionally, this review should aid in the critical evaluation of the results reported in various articles on mitochondrial potassium channels. Full article
(This article belongs to the Special Issue Intracellular Organellar Ion Channels and Molecular Physiology)
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