Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
8.1
4.9
Journals
IJMS
Special Issues
Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition
ijms-logo
Submit to Special Issue Submit Abstract to Special Issue Review for IJMS Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 20 February 2025 | Viewed by 3626

Special Issue Editors

Prof. Dr. Ewa Kurczyńska

E-Mail Website
Guest Editor
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 28 Jagiellonska St, 40-032 Katowice, Poland
Interests: cell differentiation; cell wall; auxin; somatic embryogenesis; nanoparticles; symplasmic communication
Special Issues, Collections and Topics in MDPI journals
Dr. Alexander Betekhtin

E-Mail Website
Guest Editor
Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, Poland
Interests: arabinogalactan proteins; cell cycle; cell wall; epigenetics; extensins; model plants; pectins; plant transformation; ploidy instability; proteomics; somatic embryogenesis; somaclonal variation; stem cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Modern plant cell biotechnology is a very diverse, multidisciplinary field of science. Biotechnological products are created by combining basic research in the field of biological sciences (biochemistry, molecular biology, genetics, cell biology, and immunology) and engineering sciences, and are used on a large scale in healthcare (medicine, the pharmaceutical industry, and diagnostics), agriculture, environmental protection, production (including food, chemicals, and cosmetics), and the development of alternative energy sources.

This Special Issue includes a wide range of topics linked with plant cell biotechnology, with a particular, but not exclusive, emphasis on the use of agriculturally important plants. Recent research related, for example, to genetic engineering techniques (the modification, editing, and regulation of gene expression) and other related topics are welcome.

As volume 1 of the “Modern Plant Cell Biotechnology: From Genes to Structure” Special Issue is successful, we are reopening it in the International Journal of Molecular Sciences (ISSN: 1422-0067, IF: 5.6, and JCR category Q1). This second Special Issue, “Modern Plant Cell Biotechnology: From Genes to Structure 2.0”, welcomes original manuscripts and review articles addressing this hot topic.

Prof. Dr. Ewa Kurczyńska
Dr. Alexander Betekhtin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetic engineering techniques
  • gene modification
  • gene editing
  • regulation of gene expression
  • plant cell biotechnology

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (4 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

19 pages, 8889 KiB  
Communication
Cell Wall Microdomains Analysis in the Quadrifids of Utricularia dichotoma
by Bartosz J. Płachno, Małgorzata Kapusta, Marcin Feldo and Piotr Świątek
Int. J. Mol. Sci. 2025, 26(2), 832; https://doi.org/10.3390/ijms26020832 - 20 Jan 2025
Viewed by 531
Abstract
Carnivorous plants have fascinated botanists and ecologists with their various unusual adaptations in organ structure, physiology, and complex interactions with other organisms since the time of Charles Darwin. Species of the genus Utricularia (bladderworts, family Lentibulariaceae) are carnivorous plants that prey mainly on [...] Read more.
Carnivorous plants have fascinated botanists and ecologists with their various unusual adaptations in organ structure, physiology, and complex interactions with other organisms since the time of Charles Darwin. Species of the genus Utricularia (bladderworts, family Lentibulariaceae) are carnivorous plants that prey mainly on invertebrates using traps (bladders) of leaf origin. In the traps, there are glandular trichomes called quadrifids, which produce digestive enzymes and absorb the products of prey digestion. These quadrifids are unique due to their highly complex glandular cell structure; hence, they are an excellent model for studying the cell wall and its specialization. The main aim of the study was to investigate the presence and distribution of homogalacturonans (HGs) and hemicelluloses in the cell walls of trichome cells and especially in cell wall ingrowths in the quadrifid cells. The following antibodies were used against the wall components: anti-HGs (homogalacturonans) —JIM5 (low methylesterified HGs), JIM7 (highly esterified HGs), LM19 (low methylesterified HGs), CCRC-M38 (a fully de-esterified HG), LM5 (galactan); anti-hemicelluloses—LM25 (galactoxyloglucan; XXLLG, XXLG, XXXG modules of xyloglucans), LM15 (xyloglucan), CCRC-M138 (xylan), LM11 (heteroxylan); and anti-mannans: LM20 (heteromannan) and LM22 (heteromannan). The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. In quadrifid cells, we found differences in the presence of the epitope detected by the LM5 antibody in the cell walls. In addition, cell wall ingrowths represented distinct microdomains of the cell wall in terms of the occurrence of wall components (they were methylesterified and demethylesterified homogalacturonan-poor). Hemicelluloses (galactoxyloglucan and xyloglucan) and arabinogalactans co-occur in cell wall ingrowths. Also, a part of the cell wall of the pedestal cell, which forms a Casparian strip, represented a distinct microdomain. We did not detect epitopes recognized by LM11, LM20 and LM22 antibodies. Our research shows that several cell wall microdomains occur in the cell walls of quadrifid cells. They differ depending on the presence and distribution of low methylesterified HGs, highly esterified HGs, fully de-esterified HGs, galactan (the epitope detected by the LM5 antibody), xyloglucan, galactoxyloglucan, and xylan (the epitope detected by the CCRC-M138 antibody). Full article
(This article belongs to the Special Issue Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition)
►▼ Show Figures

Figure 1

18 pages, 4445 KiB  
Article
Mechanisms of Aluminum Toxicity Impacting Root Growth in Shatian Pomelo
by Jingfu Yan, Wenbo Zhu, Dongshen Wu, Xinya Chen, Shaoxia Yang, Yingbin Xue, Ying Liu and Ying Liu
Int. J. Mol. Sci. 2024, 25(24), 13454; https://doi.org/10.3390/ijms252413454 - 15 Dec 2024
Viewed by 788
Abstract
Aluminum (Al) toxicity in acidic soils poses significant challenges to crop growth and development. However, the response mechanism of Shatian pomelo (Citrus maxima ‘Shatian Yu’) roots to Al toxicity remains poorly understood. This study employed root phenotype analysis, physiological response index measurement, [...] Read more.
Aluminum (Al) toxicity in acidic soils poses significant challenges to crop growth and development. However, the response mechanism of Shatian pomelo (Citrus maxima ‘Shatian Yu’) roots to Al toxicity remains poorly understood. This study employed root phenotype analysis, physiological response index measurement, root transcriptome analysis, and quantitative PCR (qPCR) validation to investigate the effects of Al toxicity on Shatian pomelo roots. The findings revealed that Al toxicity inhibited root growth and development, resulting in reduced root biomass, total root length, total root surface area, root volume, average root diameter, and root tip count. Antioxidant enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase activity) and soluble protein content increased with rising Al toxicity, whereas malondialdehyde content initially increased and then declined. Additionally, Al toxicity stress increased Al (1439.25%) content and decreased boron (B, 50.64%), magnesium (Mg, 42.04%), calcium (Ca, 46.02%), manganese (Mn, 86.75%), and iron (Fe, 69.92%) levels in the roots. RNA sequencing (RNA-seq) analysis identified 3855 differentially expressed genes (DEGs) between 0 mmol/L Al (control) and 4 mmol/L Al (Al toxicity) concentrations, with 1457 genes up-regulated and 2398 down-regulated, indicating a complex molecular regulatory response. The qPCR results further validated these findings. This study elucidates the response mechanisms of Shatian pomelo roots to Al toxicity stress, providing insights into the regulatory pathways involved. The findings offer valuable reference points for breeding Al-resistant Shatian pomelo varieties. The results of this study provide important genetic tools and technical support for the screening and breeding of highly resistant varieties of Shatian pomelo. On the one hand, by detecting the key indexes (such as antioxidant enzyme activity and nutrient absorption capacity) of Shatian pomelo, varieties with excellent anti-Al toxicity characteristics can be selected. On the other hand, the Al-resistant genes identified in this study, such as TFM1 and ALERTFA0, can be used to develop molecular markers, assisted marker breeding, or transgenic breeding to accelerate the breeding process of Al-resistant strains. Full article
(This article belongs to the Special Issue Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition)
►▼ Show Figures

Figure 1

24 pages, 7276 KiB  
Article
Unraveling the Genetic Control of Pigment Accumulation in Physalis Fruits
by Wennan Zhao, Haiyan Wu, Xiaohan Gao, Huimei Cai, Jiahui Zhang, Chunbo Zhao, Weishu Chen, Hongyu Qiao and Jingying Zhang
Int. J. Mol. Sci. 2024, 25(18), 9852; https://doi.org/10.3390/ijms25189852 - 12 Sep 2024
Viewed by 756
Abstract
Physalis pubescens and Physalis alkekengi, members of the Physalis genus, are valued for their delicious and medicinal fruits as well as their different ripened fruit colors—golden for P. pubescens and scarlet for P. alkekengi. This study aimed to elucidate the pigment [...] Read more.
Physalis pubescens and Physalis alkekengi, members of the Physalis genus, are valued for their delicious and medicinal fruits as well as their different ripened fruit colors—golden for P. pubescens and scarlet for P. alkekengi. This study aimed to elucidate the pigment composition and genetic mechanisms during fruit maturation in these species. Fruit samples were collected at four development stages, analyzed using spectrophotometry and high-performance liquid chromatography (HPLC), and complemented with transcriptome sequencing to assess gene expression related to pigment biosynthesis. β-carotene was identified as the dominant pigment in P. pubescens, contrasting with P. alkekengi, which contained both lycopene and β-carotene. The carotenoid biosynthesis pathway was central to fruit pigmentation in both species. Key genes pf02G043370 and pf06G178980 in P. pubescens, and TRINITY_DN20150_c1_g3, TRINITY_DN10183_c0_g1, and TRINITY_DN23805_c0_g3 in P. alkekengi were associated with carotenoid production. Notably, the MYB-related and bHLH transcription factors (TFs) regulated zeta-carotene isomerase and β-hydroxylase activities in P. pubescens with the MYB-related TF showing dual regulatory roles. In P. alkekengi, six TF families—bHLH, HSF, WRKY, M-type MADS, AP2, and NAC—were implicated in controlling carotenoid synthesis enzymes. Our findings highlight the intricate regulatory network governing pigmentation and provide insights into Physalis germplasm’s genetic improvement and conservation. Full article
(This article belongs to the Special Issue Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition)
►▼ Show Figures

Figure 1

13 pages, 2712 KiB  
Article
The REPLUMLESS Transcription Factor Controls the Expression of the RECEPTOR-LIKE CYTOPLASMIC KINASE VI_A2 Gene Involved in Shoot and Fruit Patterning of Arabidopsis thaliana
by Erzsébet Kenesi, Orsolya Beöthy-Fehér, Réka Szőllősi, Ildikó Domonkos, Ildikó Valkai and Attila Fehér
Int. J. Mol. Sci. 2024, 25(14), 8001; https://doi.org/10.3390/ijms25148001 - 22 Jul 2024
Viewed by 922
Abstract
The promoter of the RECEPTOR-LIKE CYTOPLASMIC KINASE VI_A2 (RLCK VI_A2) gene contains nine binding sites for the REPLUMLESS (RPL) transcription factor. In agreement, the expression of the kinase gene was strongly downregulated in the rpl-4 mutant. Comparing phenotypes of loss-of-function mutants, [...] Read more.
The promoter of the RECEPTOR-LIKE CYTOPLASMIC KINASE VI_A2 (RLCK VI_A2) gene contains nine binding sites for the REPLUMLESS (RPL) transcription factor. In agreement, the expression of the kinase gene was strongly downregulated in the rpl-4 mutant. Comparing phenotypes of loss-of-function mutants, it was revealed that both genes are involved in stem growth, phyllotaxis, organization of the vascular tissues, and the replum, highlighting potential functional interactions. The expression of the RLCKVI_A2 gene from the constitutive 35S promoter could not complement the rpl-4 phenotypes but exhibited a dominant positive effect on stem growth and affected vascular differentiation and organization. The results also indicated that the number of vascular bundles is regulated independently from stem thickness. Although our study cannot demonstrate a direct link between the RPL and RLVKVI_A2 genes, it highlights the significance of the proper developmental regulation of the RLCKVI_A2 promoter for balanced stem development. Full article
(This article belongs to the Special Issue Modern Plant Cell Biotechnology: From Genes to Structure, 2nd Edition)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-4
Back to TopTop