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Emerging Developments in Molecular Endocrinology and Metabolic Research

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Endocrinology and Metabolism".

Deadline for manuscript submissions: 20 January 2025 | Viewed by 1638

Special Issue Editor


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Guest Editor
1. Department of Endocrinology, Division of Medicine, Tan Tock Seng Hospital, Singapore 308433, Singapore
2. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore
3. Singapore Institute for Clinical Sciences, Brenner Centre for Molecular Medicine, Agency for Science, Technology and Research (A*STAR), Singapore 117609, Singapore
4. Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore
5. Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore
Interests: adipocyte biology; metabolic syndrome/diabetes; thyroidology; endocrine manifestations of systemic disorders; mathematical modeling of endocrine physiology
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Special Issue Information

Dear Colleagues,

The fields of endocrinology and metabolism are home to scientists and clinicians who commit themselves to dissecting and understanding the fundamental mechanisms of hormonal signaling and the regulation of complex endocrine networks in the body necessary for survival and optimal health. The range of endocrine disorders is wide and challenging to manage, with many conditions still not being fully understood and effective treatments still being deciphered. Advancements in science allow for novel, cutting-edge techniques to be developed and translated to the bedside, as seen in the historical leaps of genetic engineering which first cloned the human insulin gene in bacterial plasmids for large-scale insulin production. Gene knockouts, knock-ins and CRISPR-Cas 9 gene editing technologies interrogate disease processes with ever-increasing and finer precision and offer deep insights that can lead to new diagnostics and therapeutics strategies. This Issue is thus devoted to the exploration of exciting scientific discoveries and advances in the field of endocrine and metabolic physiology. It encompasses novel concepts, new research techniques and cutting-edge diagnostic methodologies and treatment paradigms that catalyze bench-to-bedside translation, so as to achieve better insights into the regulatory processes which orchestrate the complexities of endocrine and metabolic homeostasis. To this end, we invite experts in the field to contribute their research work to this Special Issue. It is hoped that such an endeavor will ultimately yield a book volume that will stand out as an inspiring resource for both the neophyte who wishes to be brought up to speed with the current knowledge, and also for the seasoned researcher who can benefit from the information within its pages.

Yours sincerely,

Prof. Dr. Melvin K. S. Leow
Guest Editor

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Keywords

  • endocrine feedback loops
  • endocrine and metabolic physiology
  • homeostasis
  • hormonal regulation
  • metabolic disorders
  • cellular and molecular mechanisms
  • diagnostics and therapeutics
  • techniques and methodologies
  • genomics and metabolomics
  • mathematical modeling

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Published Papers (2 papers)

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Research

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15 pages, 5181 KiB  
Article
Deciphering the Role of the SREBF1 Gene in the Transcriptional Regulation of Porcine Adipogenesis Using CRISPR/Cas9 Editing
by Mehmet Onur Aksoy, Adrianna Bilinska, Monika Stachowiak, Tatiana Flisikowska and Izabela Szczerbal
Int. J. Mol. Sci. 2024, 25(23), 12677; https://doi.org/10.3390/ijms252312677 - 26 Nov 2024
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Abstract
Sterol regulatory element-binding protein 1 (SREBP1) is an important transcription factor that controls lipid metabolism and adipogenesis. Two isoforms, SREBP1a and SREBP1c, are generated by alternative splicing of the first exon of the SREBF1 gene. The porcine SREBF1 gene has mainly been studied [...] Read more.
Sterol regulatory element-binding protein 1 (SREBP1) is an important transcription factor that controls lipid metabolism and adipogenesis. Two isoforms, SREBP1a and SREBP1c, are generated by alternative splicing of the first exon of the SREBF1 gene. The porcine SREBF1 gene has mainly been studied for its role in lipid metabolism in adipose tissues, but little is known about its involvement, and the role of its two isoforms, in adipogenesis. The aim of the present study was to introduce a deletion in the 5′-regulatory region of the SREBF1c gene, considered crucial for adipogenesis, using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) method. This approach allows for the evaluation of how inhibiting SREBF1c transcription affects the expression of other genes essential for adipocyte differentiation, particularly PPARG, CEBPA, CEBPB, CEBPD, GATA2, and FABP4. It was observed that disrupting the SREBF1c isoform had no effect on the GATA2 gene but did result in a decrease in the expression of the CEBPA and CEBPD genes, an increase in the expression of CEBPB, and an inhibition in the expression of the PPARG and FABP4 genes. These changes in gene expression blocked adipogenesis, as could be seen by the failure of lipid droplets to accumulate. Our results provide evidence highlighting the pivotal role of the SREBP1c isoform in the regulation of porcine adipogenesis. Full article
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Review

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23 pages, 1552 KiB  
Review
Oxidized Low-Density Lipoprotein and Its Role in Immunometabolism
by Negin Mosalmanzadeh and Brandt D. Pence
Int. J. Mol. Sci. 2024, 25(21), 11386; https://doi.org/10.3390/ijms252111386 - 23 Oct 2024
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Abstract
Modified cholesterols such as oxidized low-density lipoprotein (OxLDL) contribute to atherosclerosis and other disorders through the promotion of foam cell formation and inflammation. In recent years, it has become evident that immune cell responses to inflammatory molecules such as OxLDLs depend on cellular [...] Read more.
Modified cholesterols such as oxidized low-density lipoprotein (OxLDL) contribute to atherosclerosis and other disorders through the promotion of foam cell formation and inflammation. In recent years, it has become evident that immune cell responses to inflammatory molecules such as OxLDLs depend on cellular metabolic functions. This review examines the known effects of OxLDL on immunometabolism and immune cell responses in atherosclerosis and several other diseases. We additionally provide context on the relationship between OxLDL and aging/senescence and identify gaps in the literature and our current understanding in these areas. Full article
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