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The Role of MSCs on Inflammatory and Fibrotic Diseases

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (15 July 2022) | Viewed by 2884

Special Issue Editor


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Guest Editor
Department of Clinical and Molecular Sciences—Histology, Università Politecnica delle Marche, 60126 Ancona, Italy
Interests: mesenchymal stem cells; inflammation; paracrine effects; skin pathologies; miRNAs
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Special Issue Information

Dear Colleagues,

Inflammatory diseases include a vast array of disorders characterized by inflammation, ranging from asthma and autoimmune pathologies up to fibrotic diseases. Fibrosis is in fact defined as a pathological feature of most chronic inflammatory diseases. Among fibrotic diseases, pulmonary fibrosis deserves particular attention. In recent decades, scientific has research tried to address the question of the role of mesenchymal stem cells (MSCs) on inflammatory and fibrotic diseases; MSCs are able to modulate the inflamed microenvironment by secreting pro- and/or anti-inflammatory cytokines. The link between MSCs and inflammation is actually still debated: In different models, it has been observed that MSCs can sustain or counteract inflammation.

New insights about MSCs/inflammation/fibrotic diseases are requested to improve the knowledge of the mechanisms regulating these pathologies and to focus on the potential therapeutic role of MSCs.

Prof. Dr. Monia Orciani
Guest Editor

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Keywords

  • MSCs
  • inflammation
  • fibrosis
  • lung
  • cytokines
  • paracrine effect

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Published Papers (1 paper)

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Research

18 pages, 4147 KiB  
Article
Synergic Effect of Early Administration of Probiotics and Adipose-Derived Mesenchymal Stem Cells on Alleviating Inflammation-Induced Chronic Neuropathic Pain in Rodents
by Kuan-Hung Chen, Hung-Sheng Lin, Yi-Chen Li, Pei-Hsun Sung, Yi-Ling Chen, Tsung-Cheng Yin and Hon-Kan Yip
Int. J. Mol. Sci. 2022, 23(19), 11974; https://doi.org/10.3390/ijms231911974 - 9 Oct 2022
Cited by 8 | Viewed by 2300
Abstract
This study investigated the hypothesis that probiotics enhanced the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) on alleviating neuropathic pain (NP) due to chronic constriction injury (CCI) mainly through regulating the microbiota in rats. SD rats (n = 50) were categorized into [...] Read more.
This study investigated the hypothesis that probiotics enhanced the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) on alleviating neuropathic pain (NP) due to chronic constriction injury (CCI) mainly through regulating the microbiota in rats. SD rats (n = 50) were categorized into group 1 (sham-control), group 2 (NP), group 3 (NP + probiotics (i.e., 1.5 billion C.F.U./day/rat, orally 3 h after NP procedure, followed by QOD 30 times)), group 4 (NP + ADMSCs (3.0 × 105 cells) 3 h after CCI procedure, followed by QOD six times (i.e., seven times in total, i.e., mimic a clinical setting of drug use) and group 5 (NP + probiotics + ADMSCs (3.0 × 105 cells)) and euthanized by day 60 after NP induction. By day 28 after NP induction, flow-cytometric analysis showed circulating levels of early (AN-V+/PI) and late (AN-V+/PI+) apoptotic, and three inflammatory (CD11b-c+, Ly6G+ and MPO+) cells were lowest in group 1 and significantly progressively reduced in groups 2 to 5 (all p < 0.0001). By days 7, 14, 21, 28, and 60 after CCI, the thresholds of thermal paw withdrawal latency (PWL) and mechanical paw withdrawal threshold (PWT) were highest in group 1 and significantly progressively increased in groups 2 to 5 (all p < 0.0001). Numbers of pain-connived cells (Nav1.8+/peripherin+, p-ERK+/peripherin+, p-p38+/peripherin+ and p-p38+/NF200+) and protein expressions of inflammatory (p-NF-κB, IL-1ß, TNF-α and MMP-9), apoptotic (cleaved-caspase-3, cleaved-PARP), oxidative-stress (NOX-1, NOX-2), DNA-damaged (γ-H2AX) and MAPK-family (p-P38, p-JNK, p-ERK1/2) biomarkers as well as the protein levels of Nav.1.3, Nav.1.8, and Nav.1.9 in L4-L5 in dorsal root ganglia displayed an opposite pattern of mechanical PWT among the groups (all p < 0.0001). In conclusion, combined probiotic and ADMSC therapy was superior to merely one for alleviating CCI-induced NP mainly through suppressing inflammation and oxidative stress. Full article
(This article belongs to the Special Issue The Role of MSCs on Inflammatory and Fibrotic Diseases)
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