3D Bioprinting for Medical Applications

Special Issue Editors

Foundation of Research and Science Development, Warsaw, Poland
Interests: bioprinting; pancreatic islets; tissue models; pancreas; biomaterials; stem cells; medical application

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Guest Editor
Foundation of Research and Science Development, Warsaw, Poland
Interests: biomaterials; 3D bioprinting; pancreas; transplantation; tissue models; medicine

Special Issue Information

Dear Colleagues,

Bioprinting is a rapidly developing field of tissue engineering and modern regenerative medicine. Three-dimensional bioprinting using living cells and appropriate biomaterials is one of the increasingly used technologies for the regeneration of hard tissues and the production of bionic organs and tissue models. In parallel with the development of new bioprinting techniques, there is an intensive development of biomaterials and full bioink compositions that provide an appropriate environment for cells. The intensive development of new technologies and biomaterials allows us to think about bioprinting entire organs and using them in clinical practice. Bioprinted organs and smaller models may also contribute to revolutionizing preclinical testing of new drugs and active substances. The possibility of testing new drugs on bioprinted models will not only reduce the number of animals used for research, but above all, it will help us to discover new opportunities for pharmaceutical sciences and basic research. However, despite numerous studies and reports, there is still much to discover, and this requires scientists to take an interdisciplinary approach to their research. Despite advanced work and promising results of preclinical studies, the clinical use of bioprinted constructs and whole organs is still in the research phase. In particular, stem cells are being increasingly used in bioprinting research.

In this Special Issue, we would like to present a collection of articles detailing the latest developments and trends in 3D bioprinting research for medical applications, paying particular attention to the development of innovative bioinks, new cross-linking mechanisms, and cell viability and differentiation intended to create bionic constructs. In this Special Issue, we want to focus on the possibility of using these achievements in transplantation medicine, for basic and preclinical research of new drugs and the toxicity of active substances.

Dr. Marta Klak
Dr. Michal Wszoła
Guest Editors

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Keywords

  • tissue models
  • bionic organs
  • bioprinted constructs
  • regenerative medicine
  • drug testing
  • tissue-engineered products (TEP)
  • stem cells

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Published Papers (1 paper)

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Research

13 pages, 3151 KiB  
Article
Spherical Shell Bioprinting to Produce Uniform Spheroids with Controlled Sizes
by Kuk Hui Son, Dong-Ha Kim, Seunghye Park, Hyun Jae Kim, Mira Park, Seung-Jin Kim, Sang Jin Lee, Keunsun Ahn and Jin Woo Lee
J. Funct. Biomater. 2024, 15(11), 350; https://doi.org/10.3390/jfb15110350 - 18 Nov 2024
Viewed by 969
Abstract
Conventional cell spheroid production methods are largely manual, leading to variations in size and shape that compromise consistency and reliability for use in cell-based therapeutic applications. To enhance spheroid production, a spherical shell bioprinting system was implemented, enabling the high-throughput generation of uniform [...] Read more.
Conventional cell spheroid production methods are largely manual, leading to variations in size and shape that compromise consistency and reliability for use in cell-based therapeutic applications. To enhance spheroid production, a spherical shell bioprinting system was implemented, enabling the high-throughput generation of uniform cell spheroids with precisely controlled sizes. The system encapsulates cells within thin alginate hydrogel shells formed through bioprinting and ion crosslinking reactions. Alginate–calcium ion crosslinking created alginate shells that contained gelatin-based bioinks with embedded cells, facilitating spontaneous cell aggregation within the shells and eliminating the need for plastic wells. By adjusting cell concentrations in the alginate–gelatin bioink, we achieved precise control over spheroid size, maintaining a sphericity above 0.94 and size deviations within ±10 µm. This method has been successfully applied to various cell types including cancer cells, fibroblasts, chondrocytes, and epithelial cells, demonstrating its versatility. This scalable approach enhances the reliability of cell therapy and drug screening, offering a robust platform for future biomedical applications. Full article
(This article belongs to the Special Issue 3D Bioprinting for Medical Applications)
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