Journal of Functional Biomaterials

20 pages, 4201 KiB

Open AccessArticle

Impact of Particle Size and Sintering Temperature on Calcium Phosphate Gyroid Structure Scaffolds for Bone Tissue Engineering

by Romina Haydeé Aspera-Werz, Guanqiao Chen, Lea Schilonka, Islam Bouakaz, Catherine Bronne, Elisabeth Cobraiville, Grégory Nolens and Andreas Nussler

J. Funct. Biomater. 2024, 15(12), 355; https://doi.org/10.3390/jfb15120355 - 21 Nov 2024

Abstract

Due to the chemical composition and structure of the target tissue, autologous bone grafting remains the gold standard for orthopedic applications worldwide. However, ongoing advancements in alternative grafting materials show that 3D-printed synthetic biomaterials offer many advantages. For instance, they provide high availability, [...] Read more.

Due to the chemical composition and structure of the target tissue, autologous bone grafting remains the gold standard for orthopedic applications worldwide. However, ongoing advancements in alternative grafting materials show that 3D-printed synthetic biomaterials offer many advantages. For instance, they provide high availability, have low clinical limitations, and can be designed with a chemical composition and structure comparable to the target tissue. This study aimed to compare the influences of particle size and sintering temperature on the mechanical properties and biocompatibility of calcium phosphate (CaP) gyroid scaffolds. CaP gyroid scaffolds were fabricated by 3D printing using powders with the same chemical composition but different particle sizes and sintering temperatures. The physicochemical characterization of the scaffolds was performed using X-ray diffractometry, scanning electron microscopy, and microtomography analyses. The immortalized human mesenchymal stem cell line SCP-1 (osteoblast-like cells) and osteoclast-like cells (THP-1 cells) were seeded on the scaffolds as mono- or co-cultures. Bone cell attachment, number of live cells, and functionality were assessed at different time points over a period of 21 days. Improvements in mechanical properties were observed for scaffolds fabricated with narrow-particle-size-distribution powder. The physicochemical analysis showed that the microstructure varied with sintering temperature and that narrow particle size distribution resulted in smaller micropores and a smoother surface. Viable osteoblast- and osteoclast-like cells were observed for all scaffolds tested, but scaffolds produced with a smaller particle size distribution showed less attachment of osteoblast-like cells. Interestingly, low attachment of osteoclast-like cells was observed for all scaffolds regardless of surface roughness. Although bone cell adhesion was lower in scaffolds made with powder containing smaller particle sizes, the long-term function of osteoblast-like and osteoclast-like cells was superior in scaffolds with improved mechanical properties. Full article

(This article belongs to the Special Issue Biomaterials and Porous Scaffolds for Tissue Engineering and Regenerative Medicine)

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29 pages, 6045 KiB

Open AccessArticle

Green Fabrication of Silver Nanoparticles, Statistical Process Optimization, Characterization, and Molecular Docking Analysis of Their Antimicrobial Activities onto Cotton Fabrics

by Nada S. Shweqa, Noura El-Ahmady El-Naggar, Hala M. Abdelmigid, Amal A. Alyamani, Naglaa Elshafey, Hadeel El-Shall, Yasmin M. Heikal and Hoda M. Soliman

J. Funct. Biomater. 2024, 15(12), 354; https://doi.org/10.3390/jfb15120354 - 21 Nov 2024

Abstract

Nanotechnological methods for creating multifunctional fabrics are attracting global interest. The incorporation of nanoparticles in the field of textiles enables the creation of multifunctional textiles exhibiting UV irradiation protection, antimicrobial properties, self-cleaning properties and photocatalytic. Nanomaterials-loaded textiles have many innovative applications in pharmaceuticals, [...] Read more.

Nanotechnological methods for creating multifunctional fabrics are attracting global interest. The incorporation of nanoparticles in the field of textiles enables the creation of multifunctional textiles exhibiting UV irradiation protection, antimicrobial properties, self-cleaning properties and photocatalytic. Nanomaterials-loaded textiles have many innovative applications in pharmaceuticals, sports, military the textile industry etc. This study details the biosynthesis and characterization of silver nanoparticles (AgNPs) using the aqueous mycelial-free filtrate of Aspergillus flavus. The formation of AgNPs was indicated by a brown color in the extracellular filtrate and confirmed by UV-Vis spectroscopy with a peak at 426 nm. The Box-Behnken design (BBD) is used to optimize the physicochemical parameters affecting AgNPs biosynthesis. The desirability function was employed to theoretically predict the optimal conditions for the biosynthesis of AgNPs, which were subsequently experimentally validated. Through the desirability function, the optimal conditions for the maximum predicted value for the biosynthesized AgNPs (235.72 µg/mL) have been identified as follows: incubation time (58.12 h), initial pH (7.99), AgNO₃ concentration (4.84 mM/mL), and temperature (34.84 °C). Under these conditions, the highest experimental value of AgNPs biosynthesis was 247.53 µg/mL. Model validation confirmed the great accuracy of the model predictions. Scanning electron microscopy (SEM) revealed spherical AgNPs measuring 8.93–19.11 nm, which was confirmed by transmission electron microscopy (TEM). Zeta potential analysis indicated a positive surface charge (+1.69 mV), implying good stability. X-ray diffraction (XRD) confirmed the crystalline nature, while energy-dispersive X-ray spectroscopy (EDX) verified elemental silver (49.61%). Scanning electron microscopy (SEM) revealed uniformly sized spherical AgNPs. Transmission electron microscopy (TEM) revealed spherical particles measuring 8.93–19.11 nm. EDX spectrum revealed that silver is the dominant element in the AgNPs. The Zeta potential measurement revealed a positive surface charge (+1.69 mV). X-ray diffraction (XRD) confirmed the crystalline character. FTIR findings indicate the presence of phenols, proteins, alkanes, alkenes, aliphatic and aromatic amines, and alkyl groups which play significant roles in the reduction, capping, and stabilization of AgNPs. Cotton fabrics embedded with AgNPs biosynthesized using the aqueous mycelial-free filtrate of Aspergillus flavus showed strong antimicrobial activity. The disc diffusion method revealed inhibition zones of 15, 12, and 17 mm against E. coli (Gram-negative), S. aureus (Gram-positive), and C. albicans (yeast), respectively. These fabrics have potential applications in protective clothing, packaging, and medical care. In silico modeling suggested that the predicted compound derived from AgNPs on cotton fabric could inhibit Penicillin-binding proteins (PBPs) and Lanosterol 14-alpha-demethylase (L-14α-DM), with binding energies of −4.7 and −5.2 Kcal/mol, respectively. Pharmacokinetic analysis and sensitizer prediction indicated that this compound merits further investigation. Full article

(This article belongs to the Special Issue Biomaterials, Bioconjugated Materials, and Biomaterial Composites with Antimicrobial Properties)

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16 pages, 12900 KiB

Open AccessArticle

Is There an Ideal Concentration of Ozonized Oil for the Prevention and Modulation of Zoledronate-Induced Mandibular Osteonecrosis? A Study on Senescent Rats

by Mirela Caroline Silva, Izabela Fornazari Delamura, Maria Eloise de Sá Simon, Stefany Barbosa, David Tawei Ting, Karen Bechara, Jamil Awad Shibli, Carlos Fernando Mourão, Ana Paula Farnezi Bassi, Edilson Ervolino and Leonardo Perez Faverani

J. Funct. Biomater. 2024, 15(12), 353; https://doi.org/10.3390/jfb15120353 - 21 Nov 2024

Abstract

This study aimed to identify whether there is an ideal concentration for applying ozonized oil (OZ) in the post-exodontic alveoli of senescent rats treated with zoledronate (ZOL). Thirty-five female rats, aged 18 months, were divided into five groups: ZOL; ZOL+OZ500; ZOL+OZ600; ZOL+OZ700; and [...] Read more.

This study aimed to identify whether there is an ideal concentration for applying ozonized oil (OZ) in the post-exodontic alveoli of senescent rats treated with zoledronate (ZOL). Thirty-five female rats, aged 18 months, were divided into five groups: ZOL; ZOL+OZ500; ZOL+OZ600; ZOL+OZ700; and SAL. The groups treated with ZOL, and other concentrations of OZ received applications at a dose of 100 μg/kg, while the SAL group received saline. After three weeks of ZOL application, the animals underwent extraction of the lower first molar. Subsequently, local therapies were initiated: group ZOL+OZ500 at 500 mEq/kg; ZOL+Z600 at 600 mEq/kg; and ZOL+OZ700 at 700 mEq/kg at baseline, and on days 2 and 4 post-operation. Euthanasia was performed on day 28. The microtomographic parameter of bone volume and histometric data on the area of neoformed bone (NFBT) showed the highest values for the ZOL+OZ600 group (p < 0.05). All OZ groups had smaller areas of non-vital bone than the ZOL group (p < 0.05). The clinical appearance of the operated region showed the alveoli covered with soft tissue, particularly in the OZ groups. All the tested concentrations of OZ were able to prevent and modulate MRONJ. As it presents a greater amount of NFBT, the concentration of 600 mEq/kg seems to be ideal. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

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20 pages, 5133 KiB

Open AccessArticle

Strontium- and Copper-Doped Ceramic Granules in Bone Regeneration-Associated Cellular Processes

by Yuliya Safarova (Yantsen), Assem Nessipbekova, Aizhan Syzdykova, Farkhad Olzhayev, Bauyrzhan Umbayev, Aliya Kassenova, Inna V. Fadeeva, Sholpan Askarova and Julietta V. Rau

J. Funct. Biomater. 2024, 15(11), 352; https://doi.org/10.3390/jfb15110352 - 20 Nov 2024

Abstract

Background: Pathological bone fracturing is an escalating problem driven by increasing aging and obesity. Bioceramics, particularly tricalcium-phosphate-based materials (TCP), are renowned for their exceptional biocompatibility, osteoconductivity, and ability to promote biomineralization. In the present study, we designed and characterized TCP porous granules doped [...] Read more.

Background: Pathological bone fracturing is an escalating problem driven by increasing aging and obesity. Bioceramics, particularly tricalcium-phosphate-based materials (TCP), are renowned for their exceptional biocompatibility, osteoconductivity, and ability to promote biomineralization. In the present study, we designed and characterized TCP porous granules doped with strontium (Sr) and copper (Cu) (CuSr TCP). Sr²⁺ ions were selected as Sr plays a crucial role in early bone formation, osteogenesis, and angiogenesis; Cu²⁺ ions possess antibacterial properties. Materials: The synthesized CuSr TCP granules were characterized by X-ray diffraction. Cytotoxicity and cell proliferation analyses’ assays were performed through the lactate dehydrogenase (LDH) activity and CCK-8 viability tests in rat bone marrow-derived mesenchymal stem cells (BM-MSCs). Hemolytic activity was carried out with human red blood cells (RBCs). Early and late osteogenesis were assessed with alkaline phosphatase (ALP) and Alizarin Red S activity in human osteoblast progenitor cells and rat BM-MSCs. The influence of CuSr TCP on angiogenesis was investigated in human umbilical vein endothelial cells (HUVECs). Results: We have demonstrated that media enriched with CuSr TCP in concentrations ranging from 0.1 mg/mL to 1 mg/mL were not cytotoxic and did not significantly affect cell proliferation rate motility. Moreover, a concentration of 0.5 mg/mL showed a 2.5-fold increase in the migration potential of BM-MSCs. We also found that CuSr TCP-enriched media slightly increased early osteogenesis. We also found that Sr and Cu substitutions in TCP particles significantly enhanced the measured angiogenic parameters compared to control and unsubstituted TCP granules. Conclusion: Our results demonstrate that TCP porous granules doped with Sr and Cu are biocompatible, promote osteodifferentiation and angiogenesis, and could be recommended for further in vivo studies. Full article

(This article belongs to the Section Bone Biomaterials)

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11 pages, 4888 KiB

Open AccessArticle

A Novel Graphene-Based Nanomaterial for the Development of a Pelvic Implant to Treat Pelvic Organ Prolapse

by Amelia Seifalian, Alex Digesu and Vik Khullar

J. Funct. Biomater. 2024, 15(11), 351; https://doi.org/10.3390/jfb15110351 - 20 Nov 2024

Abstract

Graphene is the wonder material of the 21st century, promising cutting-edge advancements in material science with significant applications across all industries. This study investigates the use of a graphene-based nanomaterials (GBNs) ans trade-registered Hastalex^®, as novel materials for surgical implants aimed [...] Read more.

Graphene is the wonder material of the 21st century, promising cutting-edge advancements in material science with significant applications across all industries. This study investigates the use of a graphene-based nanomaterials (GBNs) ans trade-registered Hastalex^®, as novel materials for surgical implants aimed at treating pelvic organ prolapse (POP). This study investigates the mechanical properties and physicochemical characteristics of the material, mainly focusing on its potential to address the limitations of existing polypropylene (PP) implants, which has been associated with numerous complications and banned across multiple countries. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) confirmed the bonding between functionalised graphene oxide (FGO) and the base polymer chain. Hastalex exhibited excellent mechanical properties with 58 N/mm² maximum tensile strength at break and 701% elongation at break, whilst maintaining its shape with no plastic deformation. These results were comparable to that of sheep pelvic muscular tissue. Hastalex demonstrated its hydrophilic properties from contact angle measurements. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed a uniform plane with surface nanotopography, promoting cell-to-material interaction. The results confirmed the suitability of Hastalex in the development of a new pelvic membrane to treat POP. Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

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13 pages, 3151 KiB

Open AccessArticle

Spherical Shell Bioprinting to Produce Uniform Spheroids with Controlled Sizes

by Kuk Hui Son, Dong-Ha Kim, Seunghye Park, Hyun Jae Kim, Mira Park, Seung-Jin Kim, Sang Jin Lee, Keunsun Ahn and Jin Woo Lee

J. Funct. Biomater. 2024, 15(11), 350; https://doi.org/10.3390/jfb15110350 - 18 Nov 2024

Abstract

Conventional cell spheroid production methods are largely manual, leading to variations in size and shape that compromise consistency and reliability for use in cell-based therapeutic applications. To enhance spheroid production, a spherical shell bioprinting system was implemented, enabling the high-throughput generation of uniform [...] Read more.

Conventional cell spheroid production methods are largely manual, leading to variations in size and shape that compromise consistency and reliability for use in cell-based therapeutic applications. To enhance spheroid production, a spherical shell bioprinting system was implemented, enabling the high-throughput generation of uniform cell spheroids with precisely controlled sizes. The system encapsulates cells within thin alginate hydrogel shells formed through bioprinting and ion crosslinking reactions. Alginate–calcium ion crosslinking created alginate shells that contained gelatin-based bioinks with embedded cells, facilitating spontaneous cell aggregation within the shells and eliminating the need for plastic wells. By adjusting cell concentrations in the alginate–gelatin bioink, we achieved precise control over spheroid size, maintaining a sphericity above 0.94 and size deviations within ±10 µm. This method has been successfully applied to various cell types including cancer cells, fibroblasts, chondrocytes, and epithelial cells, demonstrating its versatility. This scalable approach enhances the reliability of cell therapy and drug screening, offering a robust platform for future biomedical applications. Full article

(This article belongs to the Special Issue 3D Bioprinting for Medical Applications)

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2 pages, 351 KiB

Open AccessCorrection

Correction: Jin et al. A pH-Responsive DNA Tetrahedron/Methotrexate Drug Delivery System Used for Rheumatoid Arthritis Treatment. J. Funct. Biomater. 2023, 14, 541

by Yi Jin, Xingyu Ge, Yinjin Xu, Siyi Wang, Qian Lu, Aidong Deng, Jingjing Li and Zhifeng Gu

J. Funct. Biomater. 2024, 15(11), 349; https://doi.org/10.3390/jfb15110349 - 18 Nov 2024

Abstract

In the original publication [...] Full article

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34 pages, 2191 KiB

Open AccessReview

Properties, Production, and Recycling of Regenerated Cellulose Fibers: Special Medical Applications

by Sandra Varnaitė-Žuravliova and Julija Baltušnikaitė-Guzaitienė

J. Funct. Biomater. 2024, 15(11), 348; https://doi.org/10.3390/jfb15110348 - 16 Nov 2024

Abstract

Regenerated cellulose fibers are a highly adaptable biomaterial with numerous medical applications owing to their inherent biocompatibility, biodegradability, and robust mechanical properties. In the domain of wound care, regenerated cellulose fibers facilitate a moist environment conducive to healing, minimize infection risk, and adapt [...] Read more.

Regenerated cellulose fibers are a highly adaptable biomaterial with numerous medical applications owing to their inherent biocompatibility, biodegradability, and robust mechanical properties. In the domain of wound care, regenerated cellulose fibers facilitate a moist environment conducive to healing, minimize infection risk, and adapt to wound topographies, making it ideal for different types of dressings. In tissue engineering, cellulose scaffolds provide a matrix for cell attachment and proliferation, supporting the development of artificial skin, cartilage, and other tissues. Furthermore, regenerated cellulose fibers, used as absorbable sutures, degrade within the body, eliminating the need for removal and proving advantageous for internal suturing. The medical textile industry relies heavily on regenerated cellulose fibers because of their unique properties that make them suitable for various applications, including wound care, surgical garments, and diagnostic materials. Regenerated cellulose fibers are produced by dissolving cellulose from natural sources and reconstituting it into fiber form, which can be customized for specific medical uses. This paper will explore the various types, properties, and applications of regenerated cellulose fibers in medical contexts, alongside an examination of its manufacturing processes and technologies, as well as associated challenges. Full article

(This article belongs to the Special Issue Biodegradable Polymers and Textiles)

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14 pages, 2811 KiB

Open AccessArticle

Carbon Dot Micelles Synthesized from Leek Seeds in Applications for Cobalt (II) Sensing, Metal Ion Removal, and Cancer Therapy

by Teh-Hua Tsai, Wei Lo, Hsiu-Yun Wang and Tsung-Lin Tsai

J. Funct. Biomater. 2024, 15(11), 347; https://doi.org/10.3390/jfb15110347 - 15 Nov 2024

Abstract

Popular photoluminescent (PL) nanomaterials, such as carbon dots, have attracted substantial attention from scientists due to their photophysical properties, biocompatibility, low cost, and diverse applicability. Carbon dots have been used in sensors, cell imaging, and cancer therapy. Leek seeds with anticancer, antimicrobial, and [...] Read more.

Popular photoluminescent (PL) nanomaterials, such as carbon dots, have attracted substantial attention from scientists due to their photophysical properties, biocompatibility, low cost, and diverse applicability. Carbon dots have been used in sensors, cell imaging, and cancer therapy. Leek seeds with anticancer, antimicrobial, and antioxidant functions serve as traditional Chinese medicine. However, leek seeds have not been studied as a precursor of carbon dots. In this study, leek seeds underwent a supercritical fluid extraction process. Leek seed extract was obtained and then carbonized using a dry heating method, followed by hydrolysis to form carbon dot micelles (CD-micelles). CD-micelles exhibited analyte-induced PL quenching against Co²⁺ through the static quenching mechanism, with the formation of self-assembled Co²⁺-CD-micelle sphere particles. In addition, CD-micelles extracted metal ion through liquid–liquid extraction, with removal efficiencies of >90% for Pb²⁺, Al³⁺, Fe³⁺, Cr³⁺, Pd²⁺, and Au³⁺. Moreover, CD-micelles exhibited ABTS•⁺ radical scavenging ability and cytotoxicity for cisplatin-resistant lung cancer cells. CD-micelles killed cisplatin-resistant small-cell lung cancer cells in a dose-dependent manner with a cancer cell survival rate down to 12.8 ± 4.2%, with a similar treatment function to that of cisplatin. Consequently, CD-micelles functionalized as novel antioxidants show great potential as anticancer nanodrugs in cancer treatment. Full article

(This article belongs to the Section Biomaterials for Cancer Therapies)

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14 pages, 6217 KiB

Open AccessArticle

The Effects of Surface Patterning and Photobiomodulation on the Osseointegration of Titanium Implants in Osteoporotic Long Bones: An In Vivo Study in Rats

by Theodor Popa, Mircea Negrutiu, Luciana Madalina Gherman, Alina Deniza Ciubean, Dan Ionut Cosma, Dan Gheban, Catalin Popa and Laszlo Irsay

J. Funct. Biomater. 2024, 15(11), 346; https://doi.org/10.3390/jfb15110346 - 14 Nov 2024

Abstract

This study aimed to assess the impact of titanium surface patterning used in combination with photobiomodulation therapy on enhancing osseointegration in osteoporotic bone fractures. C.p. titanium implants were employed, half with an unmodified surface and half with a modified one, showing a nanostructured [...] Read more.

This study aimed to assess the impact of titanium surface patterning used in combination with photobiomodulation therapy on enhancing osseointegration in osteoporotic bone fractures. C.p. titanium implants were employed, half with an unmodified surface and half with a modified one, showing a nanostructured cellular surface. Surface patterning aimed to obtain a complex morphology designed for better osseointegration, using a selective anodization process after photoresist coating. A total of 52 rats were used, of which 4 were sacrificed 12 weeks after ovariectomy to evaluate bone density. A total of 48 rats received titanium implants in both tibiae and underwent surgery for implant placement and bone fracture. Half of the rats were subjected to photobiomodulation. The times of sacrifice were 2, 4, and 6 weeks after finalizing LASER therapy. The evaluation methods were micro-CT scanning, the mechanical pull-force test, and morphology. Mechanical tests revealed a significant difference in the surface-patterned titanium with the LASER group at 2 weeks, but not at 4 and 6 weeks. This group outperformed regular titanium and titanium/LASER groups. Micro-CT showed no significant differences, while the morphology indicated better bone quality at 4 weeks in all LASER-treated groups. The effect of surface patterning and photobiomodulation leads to better osseointegration, especially in the earlier stages. Full article

(This article belongs to the Special Issue Functional Composites for Bone Implants and Osseointegration)

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20 pages, 1556 KiB

Open AccessReview

Spheroid-Exosome-Based Bioprinting Technology in Regenerative Medicine

by Hwa-Yong Lee and Jin Woo Lee

J. Funct. Biomater. 2024, 15(11), 345; https://doi.org/10.3390/jfb15110345 - 14 Nov 2024

Abstract

Since the discovery that exosomes can exchange genes, their potential use as tools for tissue regeneration, disease diagnosis, and therapeutic applications has drawn significant attention. Emerging three-dimensional (3D) printing technologies, such as bioprinting, which allows the printing of cells, proteins, DNA, and other [...] Read more.

Since the discovery that exosomes can exchange genes, their potential use as tools for tissue regeneration, disease diagnosis, and therapeutic applications has drawn significant attention. Emerging three-dimensional (3D) printing technologies, such as bioprinting, which allows the printing of cells, proteins, DNA, and other biological materials, have demonstrated the potential to create complex body tissues or personalized 3D models. The use of 3D spheroids in bioprinting facilitates volumetric tissue reconstruction and accelerates tissue regeneration via exosome secretion. In this review, we discussed a convergence approach between two promising technologies for bioprinting and exosomes in regenerative medicine. Among the various 3D cell culture methods used for exosome production, we focused on spheroids, which are suitable for mass production by bioprinting. We then summarized the research results on cases of bioprinting applications using the spheroids and exosomes produced. If a large number of spheroids can be supplied through bioprinting, the spheroid-exosome-based bioprinting technology will provide new possibilities for application in tissue regeneration, disease diagnosis, and treatment. Full article

(This article belongs to the Special Issue Advanced Technologies for Processing Functional Biomaterials)

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15 pages, 6365 KiB

Open AccessArticle

Finite Element Combined Design and Material Optimization Addressing the Wear in Removable Implant Prosthodontics

by Pejman Shayanfard, Xingchen Tan, Matthias Karl and Frank Wendler

J. Funct. Biomater. 2024, 15(11), 344; https://doi.org/10.3390/jfb15110344 - 14 Nov 2024

Abstract

Wear at the male–female interface of retentive elements in implant-supported removable prostheses is the most frequent complication in such applications. The lack of an ideal/optimal insertion path, as well as the fabrication inaccuracies, are the primary contributors to this issue. A male attachment [...] Read more.

Wear at the male–female interface of retentive elements in implant-supported removable prostheses is the most frequent complication in such applications. The lack of an ideal/optimal insertion path, as well as the fabrication inaccuracies, are the primary contributors to this issue. A male attachment with a common ball anchor enhanced by lateral flexibility was investigated as a solution, compared to the widely used rigid ball anchor design. A parametric finite element analysis was performed to compare the wear-inducing maximum strain at the female polymer counterpart by various attachment designs made from titanium and Nitinol. The evolution of mechanical strains causing wear in the female part, as well as the contribution of stresses and martensitic transformation in the implant’s flexible shaft, were evaluated under several insertion misfit scenarios. Results indicate that introducing a long flexible shaft in the titanium implant reduced maximum strains in the female attachment part by up to 61% as compared to the solid ball anchor. Further improvement was observed by using the shape memory alloy Nitinol as shaft material, leading to a minor reduction in stress and strain at the contact surface but allowing for a shorter abutment. Finally, the optimized Nitinol implant design with a short, necked flexible shaft promoting martensitic transformation at low plateau stress resulted in an approximate 90% reduction in maximum strains at the inner surface of the female part during manual insertion, which indicates a significantly reduced wear phenomenon at the contact. Full article

(This article belongs to the Special Issue Biomaterials and Biomechanics Modelling in Dental Implantology)

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12 pages, 3319 KiB

Open AccessArticle

The Effect of Micro-Computed Tomography Thresholding Methods on Bone Micromorphometric Analysis

by Arda Buyuksungur, Bence Tamás Szabó, Adrienn Dobai and Kaan Orhan

J. Funct. Biomater. 2024, 15(11), 343; https://doi.org/10.3390/jfb15110343 - 13 Nov 2024

Abstract

Bone micromorphometric parameters are generally analyzed with micro CT to reveal two- and three-dimensional structures. These parameters are generally used for new bone formation studies such as tissue engineering and biomaterials studies. Different threshold methods are used for the image segmentation of bone [...] Read more.

Bone micromorphometric parameters are generally analyzed with micro CT to reveal two- and three-dimensional structures. These parameters are generally used for new bone formation studies such as tissue engineering and biomaterials studies. Different threshold methods are used for the image segmentation of bone micromorphometric parameters. However, these different threshold methods provide different results for the bones analyzed. This study aimed to compare thresholding methods to evaluate bone micromorphometric parameters in the mouse bone. A dataset containing 15 mouse tibia was used to analyze the different thresholding methods for bone micromorphometric parameter analysis. These threshold methods were used to analyze the mouse tibia (n = 15) with thresholded bones. The threshold methods and the analysis were used directly from CTAn (Bruker Micro-CT). The results were compared between the threshold methods, which included bone volume, trabecular number, connectivity, trabecular separation, and other parameters. There was agreement to some extent for all bone micromorphometric analyses using the different thresholding methods. The results showed that the thresholding method showed good agreement for connectivity and trabecular thickness, but the other parameters showed limited agreement. The evaluation of threshold methods allows for the comparison of image segmentation and the quantification of mouse tibia micromorphometric parameters. This study may enable the analysis of bone micromorphometric parameters using the relatively close threshold method in image segmentation across different research groups. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

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10 pages, 2415 KiB

Open AccessArticle

Neutrophils Respond Selectively to Physical Cues: Roughness Modulates Its Granule Release, and NETosis

by Gayathiri Elangovan, Daniel J. Fernandes, Andrew Cameron, Souptik Basu, Joao Martins De Mello Neto, Anna Peishan Jiang, Peter Reher, Stephen Hamlet and Carlos Marcelo S. Figueredo

J. Funct. Biomater. 2024, 15(11), 342; https://doi.org/10.3390/jfb15110342 - 13 Nov 2024

Abstract

Our study examined how different titanium alloy Ti6Al4V (Ti64) and zirconia (ZrO₂) surfaces, ranging from rough to very smooth, affect the expression of elastase (NE), matrix metalloproteinase (MMP)-8, MMP-9, and extracellular traps (NETs) by neutrophils. Discs of Ti64 and ZrO₂ [...] Read more.

Our study examined how different titanium alloy Ti6Al4V (Ti64) and zirconia (ZrO₂) surfaces, ranging from rough to very smooth, affect the expression of elastase (NE), matrix metalloproteinase (MMP)-8, MMP-9, and extracellular traps (NETs) by neutrophils. Discs of Ti64 and ZrO₂, 10 mm in diameter and 1.5 mm thick, were created using diamond-impregnated polishing burs and paste to produce rough (Ra > 3 µm), smooth (Ra ≥ 1 to 1.5 µm), and very smooth (Ra < 0.1 µm) surfaces. Neutrophils from Wistar rats were cultured on these surfaces, and the culture supernatants were then examined for NE, MMP-8, and MMP-9 using ELISA. At the same time, NET formation was demonstrated immunohistochemically by staining neutrophils with CD16b and DNA with DAPI. Overall, the expressions of NE and MMP-8 were significantly higher from neutrophil culture on Ti64 and ZrO₂ rough surfaces compared to the very smooth surface (R > S > VS) after 2 h and 4 h of culture. The expression of MMP-9 also increased with culture time; however, no significant surface effects on expression were observed. Similarly, rough Ti64 and ZrO₂ surfaces (R & S) also showed significantly larger NET formation compared to the very smooth surface (VS) after 4 h and 8 h cultures. Our findings suggest that increasing surface roughness on Ti64 and ZrO₂ triggers higher NE, MMP-8, and NET formation secretion. Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

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13 pages, 4105 KiB

Open AccessArticle

In Vitro Microscopical and Microbiological Assessment of the Sealing Ability of Calcium Silicate-Based Root Canal Sealers

by Karin Christine Huth, Sabina Noreen Wuersching, Leander Benz, Stefan Kist and Maximilian Kollmuss

J. Funct. Biomater. 2024, 15(11), 341; https://doi.org/10.3390/jfb15110341 - 12 Nov 2024

Abstract

This in vitro study evaluated the sealing ability and microleakage of calcium silicate-based sealers compared to an epoxy resin-based sealer. One hundred twenty-five roots from anterior teeth were chemo-mechanically prepared and divided into four groups: AH Plus (AH), ProRoot MTA (PR), Medcem MTA [...] Read more.

This in vitro study evaluated the sealing ability and microleakage of calcium silicate-based sealers compared to an epoxy resin-based sealer. One hundred twenty-five roots from anterior teeth were chemo-mechanically prepared and divided into four groups: AH Plus (AH), ProRoot MTA (PR), Medcem MTA (MC), and Total Fill BC Sealer/BC-coated gutta-percha (TF); n = 30. Confocal laser scanning microscopy was used to measure sealer penetration at three horizontal levels in 10 roots per group, while glucose leakage over 30 days was assessed in 20 roots. A lateral compaction technique was used for most groups, except for TF, which employed a single-cone method. Data were analyzed using Python with a Kruskal–Wallis test and Dunn’s post hoc test. TF showed significantly greater penetration in the coronal and middle sections, while PR had the least penetration in the apical section. PR exhibited the highest canal circumference penetration, especially compared to MC and TF. Glucose leakage increased over time in all groups, with TF showing the highest permeability after 30 days. Overall, calcium silicate-based sealers PR, MC, and TF performed similarly to the epoxy resin standard AH, with all groups exhibiting decreasing penetration from coronal to apical and increased leakage over time. Full article

(This article belongs to the Special Issue Advanced Materials for Clinical Endodontic Applications (2nd Edition))

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34 pages, 4136 KiB

Open AccessReview

Synthesis, Functionalization, and Biomedical Applications of Iron Oxide Nanoparticles (IONPs)

by Mostafa Salehirozveh, Parisa Dehghani and Ivan Mijakovic

J. Funct. Biomater. 2024, 15(11), 340; https://doi.org/10.3390/jfb15110340 - 12 Nov 2024

Abstract

Iron oxide nanoparticles (IONPs) have garnered significant attention in biomedical applications due to their unique magnetic properties, biocompatibility, and versatility. This review comprehensively examines the synthesis methods, surface functionalization techniques, and diverse biomedical applications of IONPs. Various chemical and physical synthesis techniques, including [...] Read more.

Iron oxide nanoparticles (IONPs) have garnered significant attention in biomedical applications due to their unique magnetic properties, biocompatibility, and versatility. This review comprehensively examines the synthesis methods, surface functionalization techniques, and diverse biomedical applications of IONPs. Various chemical and physical synthesis techniques, including coprecipitation, sol–gel processes, thermal decomposition, hydrothermal synthesis, and sonochemical routes, are discussed in detail, highlighting their advantages and limitations. Surface functionalization strategies, such as ligand exchange, encapsulation, and silanization, are explored to enhance the biocompatibility and functionality of IONPs. Special emphasis is placed on the role of IONPs in biosensing technologies, where their magnetic and optical properties enable significant advancements, including in surface-enhanced Raman scattering (SERS)-based biosensors, fluorescence biosensors, and field-effect transistor (FET) biosensors. The review explores how IONPs enhance sensitivity and selectivity in detecting biomolecules, demonstrating their potential for point-of-care diagnostics. Additionally, biomedical applications such as magnetic resonance imaging (MRI), targeted drug delivery, tissue engineering, and stem cell tracking are discussed. The challenges and future perspectives in the clinical translation of IONPs are also addressed, emphasizing the need for further research to optimize their properties and ensure safety and efficacy in medical applications. This review aims to provide a comprehensive understanding of the current state and future potential of IONPs in both biosensing and broader biomedical fields. Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

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12 pages, 424 KiB

Open AccessSystematic Review

Propolis in Dental Implantology: A Systematic Review of Its Effects and Benefits

by Magdalena Sycińska-Dziarnowska, Liliana Szyszka-Sommerfeld, Magdalena Ziąbka, Krzysztof Woźniak and Gianrico Spagnuolo

J. Funct. Biomater. 2024, 15(11), 339; https://doi.org/10.3390/jfb15110339 - 12 Nov 2024

Abstract

Dental implants are widely recognized for their effectiveness in restoring missing teeth, yet their success is often compromised by infections or inadequate osseointegration. Propolis, a natural resinous substance with potent antimicrobial, anti-inflammatory, and osteogenic properties, has emerged as a promising adjunct in dental [...] Read more.

Dental implants are widely recognized for their effectiveness in restoring missing teeth, yet their success is often compromised by infections or inadequate osseointegration. Propolis, a natural resinous substance with potent antimicrobial, anti-inflammatory, and osteogenic properties, has emerged as a promising adjunct in dental implantology. This systematic review critically evaluates the current evidence on the incorporation of propolis into dental implants, focusing on its impact on antimicrobial efficacy, bone healing, and overall implant stability. The study protocol was registered in PROSPERO under the registration number CRD42024577122. The PRISMA diagram visually represented the search strategy, screening, and inclusion process. Two reviewers conducted a comprehensive literature search across five databases: PubMed, PubMed Central, Embase, Scopus, and Web of Science. The review synthesized findings from 13 studies; in vitro, in vivo, and clinical studies, highlighting that propolis significantly enhances antibacterial and antifungal activities against pathogens such as Staphylococcus aureus, Candida albicans, and Streptococcus mutans, thereby reducing the risk of peri-implant infections. Additionally, propolis promotes osseointegration by stimulating osteoblast activity and reducing inflammatory cytokine expression, leading to improved bone formation and implant stability. The anti-inflammatory and antioxidant properties of propolis further contribute to a favorable healing environment, enhancing the long-term success of dental implants. The systematic review underscores the potential of propolis as a safe, biocompatible, and effective material for improving dental implant outcomes. However, it also identifies the need for more extensive clinical trials to fully establish standardized protocols for propolis application in implantology. This review provides an overview of propolis’s potential role in dental implants and suggests promising avenues for future research to optimize its benefits in clinical practice. Full article

(This article belongs to the Special Issue Biomaterials in Dentistry 2024)

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12 pages, 2128 KiB

Open AccessArticle

Marginal Discrepancy and Internal Fit of 3D-Printed Versus Milled Laminate Veneers: An In Vitro Study

by Arwa Daghrery, Honey Lunkad, Khalid Mobarki, Majed Alhazmi, Hussain Khubrani, Thilla Sekar Vinothkumar and Eman Jabarti

J. Funct. Biomater. 2024, 15(11), 338; https://doi.org/10.3390/jfb15110338 - 11 Nov 2024

Abstract

The clinical success of laminate veneers depends upon their marginal discrepancy (MD) and internal gap (IG). This study aims to compare and evaluate the MD, overall discrepancy (OD), absolute marginal discrepancy (AMD), and IG of 3D-printed (Varseosmile TrinQ and Varseosmile Crown Plus) and [...] Read more.

The clinical success of laminate veneers depends upon their marginal discrepancy (MD) and internal gap (IG). This study aims to compare and evaluate the MD, overall discrepancy (OD), absolute marginal discrepancy (AMD), and IG of 3D-printed (Varseosmile TrinQ and Varseosmile Crown Plus) and computer-aided design and manufacturing (CAD/CAM)-milled (Brilliant Crios) laminate veneers using cone-beam computed tomography (CBCT). Thirty maxillary central incisors were prepared and divided randomly into three groups (n = 10). All teeth were scanned for veneer fabrication: Group PVT teeth with 3D-printed Varseosmile TrinQ, Group PVC teeth with 3D-printed Varseosmile Crown Plus, and Group BCM teeth with Brilliant Crios milled veneers. The specimens with respective veneers were scanned using CBCT, and the sectional images were measured for IG, MD, OD, and AMD. The mean values for MD recorded were 0.27, 0.31, and 0.40 for PVT, PVC, and BCM respectively). The mean values for IG recorded were as follows: PVT group—0.24, PVC group—0.28, and BCM group—0.39, and those for OD were as follows: PVT—0.22, PVC—0.32, and BCM—0.41. Intragroup significance was observed for IG and OD (p = 0.001). Findings revealed that milled veneers have a higher IG and MD than 3D-printed veneers, making them less clinically acceptable. Full article

(This article belongs to the Special Issue CAD–CAM and Bioactive Glass for Restorative and Prosthetic Dentistry)

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Graphical abstract

11 pages, 2744 KiB

Open AccessArticle

Enhancing the Biological Properties of Organic–Inorganic Hybrid Calcium Silicate Cements: An In Vitro Study

by Minji Choi, Jiyoung Kwon, Ji-Hyun Jang, Duck-Su Kim and Hyun-Jung Kim

J. Funct. Biomater. 2024, 15(11), 337; https://doi.org/10.3390/jfb15110337 - 10 Nov 2024

Abstract

(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and [...] Read more.

(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs). (2) Methods: Proroot MTA was supplemented with 1–5 wt% 63S BAG and 10 wt% ELP. The experimental groups contained various combinations of HSCS with ELP and BAG. Cell viability was assessed using an MTT assay, cell migration was evaluated using wound healing and transwell assays, and osteogenic activity was determined through Alizarin Red S staining and a gene expression analysis of osteogenic markers (ALP, RUNX-2, OCN, and Col1A2). (3) Results: The combination of ELP and BAG significantly enhanced the viability of hPDLFs with an optimal BAG concentration of 1–4%. Cell migration assays demonstrated faster migration rates in groups with 2–4% BAG and ELP incorporation. Osteogenic activity was the highest with 2–3% BAG incorporation with ELP, as evidenced by intense Alizarin Red S staining and the upregulation of osteogenic differentiation markers. (4) Conclusions: The incorporation of ELP (organic) and BAG (inorganic) into HCSC significantly enhances the viability, migration, and osteogenic differentiation of hPDLFs. These findings suggest that composite HCSC might support healing in destructed bone lesions in endodontics. Full article

(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry)

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