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Fungal-Related Proteomics in Biotechnology and Health
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Fungal-Related Proteomics in Biotechnology and Health

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Genomics, Genetics and Molecular Biology".

Deadline for manuscript submissions: 30 November 2024 | Viewed by 6208

Special Issue Editor

Prof. Dr. Carlos André Ornelas Ricart

E-Mail Website
Guest Editor
Department of Cell Biology, University of Brasília, Brasilia, Brazil
Interests: biochemistry; protein chemistry; proteomic

Special Issue Information

Dear Colleagues,

The Special Issue entitled "Fungal-Related Proteomics in Biotechnology and Health" aims to present recent research that studies the biology of fungi using a proteomics approach. Articles that deal with fungi–bacteria complexes will also be accepted.

Topics of interest include, but are not limited to, the following:

  1. Proteomics of filamentous fungi related to plant-biomass decomposition;
  2. Proteomics of pathogenic fungi;
  3. Characterization of multienzymatic complexes secreted by fungi and bacteria;
  4. Proteomics of pathogen–host interaction;
  5. Methodologies of proteomics applied to fungi;
  6. Characterization of biomass-degrading enzyme repertoires produced by filamentous fungi;
  7. Proteomics and bioremediation.

Reviews, original research, and communications are welcome.

Prof. Dr. Carlos André Ornelas Ricart
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (4 papers)

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Research

17 pages, 3309 KiB  
Article
Exploring the Synergistic Secretome: Insights from Co-Cultivation of Aspergillus brasiliensis and Trichoderma reesei RUT-C30
by Guilherme Bento Sperandio, Reynaldo Magalhães Melo, Taísa Godoy Gomes, Robert Neil Gerard Miller, Luis Henrique Ferreira do Vale, Marcelo Valle de Sousa, Carlos André Ornelas Ricart and Edivaldo Ximenes Ferreira Filho
J. Fungi 2024, 10(10), 677; https://doi.org/10.3390/jof10100677 - 28 Sep 2024
Viewed by 674
Abstract
The spectrum of enzymes required for complete lignocellulosic waste hydrolysis is too diverse to be secreted by a single organism. An alternative is to employ fungal co-cultures to obtain more diverse and complete enzymatic cocktails without the need to mix enzymes during downstream [...] Read more.
The spectrum of enzymes required for complete lignocellulosic waste hydrolysis is too diverse to be secreted by a single organism. An alternative is to employ fungal co-cultures to obtain more diverse and complete enzymatic cocktails without the need to mix enzymes during downstream processing. This study evaluated the co-cultivation of Aspergillus brasiliensis and Trichoderma reesei RUT-C30 in different conditions using sugarcane bagasse as the carbon source. The resulting enzymatic cocktails were characterized according to the impact of strain inoculation time on enzymatic activities and proteome composition. Data revealed that the profile of each enzymatic extract was highly dependent on the order in which the participating fungi were inoculated. Some of the co-cultures exhibited higher enzyme activities compared to their respective monocultures for enzymes such as CMCase, pectinase, β-glucosidase, and β-xylosidase. Analysis of the T. reesei RUT-C30 and A. brasiliensis co-culture secretome resulted in the identification of 167 proteins, with 78 from T. reesei and 89 from A. brasiliensis. In agreement with the enzymatic results, proteome analysis also revealed that the timing of inoculation greatly influences the overall secretome, with a predominance of T. reesei RUT-C30 proteins when first inoculated or in simultaneous inoculation. Full article
(This article belongs to the Special Issue Fungal-Related Proteomics in Biotechnology and Health)
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20 pages, 6538 KiB  
Article
An Enzymatic and Proteomic Analysis of Panus lecomtei during Biodegradation of Gossypol in Cottonseed
by Clemente Batista Soares Neto, Taísa Godoy Gomes, Edivaldo Ximenes Ferreira Filho, Wagner Fontes, Carlos André Ornelas Ricart, João Ricardo Moreira de Almeida, Félix Gonçalves de Siqueira and Robert Neil Gerard Miller
J. Fungi 2024, 10(5), 321; https://doi.org/10.3390/jof10050321 - 27 Apr 2024
Cited by 1 | Viewed by 1670
Abstract
Cotton is an important plant-based protein. Cottonseed cake, a byproduct of the biodiesel industry, offers potential in animal supplementation, although the presence of the antinutritional sesquiterpenoid gossypol limits utilization. The macrofungus Panus lecomtei offers potential in detoxification of antinutritional factors. Through an enzymatic [...] Read more.
Cotton is an important plant-based protein. Cottonseed cake, a byproduct of the biodiesel industry, offers potential in animal supplementation, although the presence of the antinutritional sesquiterpenoid gossypol limits utilization. The macrofungus Panus lecomtei offers potential in detoxification of antinutritional factors. Through an enzymatic and proteomic analysis of P. lecomtei strain BRM044603, grown on crushed whole cottonseed contrasting in the presence of free gossypol (FG), this study investigated FG biodegradation over a 15-day cultivation period. Fungal growth reduced FG to levels at 100 μg/g, with a complex adaptive response observed, involving primary metabolism and activation of oxidative enzymes for metabolism of xenobiotics. Increasing activity of secreted laccases correlated with a reduction in FG, with enzyme fractions degrading synthetic gossypol to trace levels. A total of 143 and 49 differentially abundant proteins were observed across the two contrasting growth conditions after 6 and 12 days of cultivation, respectively, revealing a dynamic protein profile during FG degradation, initially related to constitutive metabolism, then later associated with responses to oxidative stress. The findings advance our understanding of the mechanisms involved in gossypol degradation and highlight the potential of P. lecomtei BRM044603 in cotton waste biotreatment, relevant for animal supplementation, sustainable resource utilization, and bioremediation. Full article
(This article belongs to the Special Issue Fungal-Related Proteomics in Biotechnology and Health)
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17 pages, 4983 KiB  
Article
Combined Proteomic and Metabolomic Analyses Reveal the Comprehensive Regulation of Stropharia rugosoannulata Mycelia Exposed to Cadmium Stress
by Qin Dong, Mingjie Chen, Changxia Yu, Yaru Zhang, Lei Zha, Pattana Kakumyan, Huanling Yang and Yan Zhao
J. Fungi 2024, 10(2), 134; https://doi.org/10.3390/jof10020134 - 7 Feb 2024
Viewed by 1699
Abstract
The potential of Stropharia rugosoannulata as a microbial remediation material for cadmium (Cd)-contaminated soil lies in its capacity to absorb and accumulate Cd in its mycelia. This study utilized the TMT and LC−MS techniques to conduct integrated proteomic and metabolomic analyses with the [...] Read more.
The potential of Stropharia rugosoannulata as a microbial remediation material for cadmium (Cd)-contaminated soil lies in its capacity to absorb and accumulate Cd in its mycelia. This study utilized the TMT and LC−MS techniques to conduct integrated proteomic and metabolomic analyses with the aim of investigating the mycelial response mechanisms of S. rugosoannulata under low- and high-Cd stresses. The results revealed that mycelia employed a proactive defense mechanism to maintain their physiological functions, leading to reduced sensitivity to low-Cd stress. The ability of mycelia to withstand high levels of Cd stress was influenced primarily by the comprehensive regulation of six metabolic pathways, which led to a harmonious balance between nitrogen and carbohydrate metabolism and to reductions in oxidative stress and growth inhibition caused by Cd. The results provide valuable insights into the molecular mechanisms involved in the response of S. rugosoannulata mycelia to Cd stress. Full article
(This article belongs to the Special Issue Fungal-Related Proteomics in Biotechnology and Health)
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18 pages, 2128 KiB  
Article
The Response of Paracoccidioides lutzii to the Interaction with Human Neutrophils
by Lana O’Hara Souza Silva, Lilian Cristiane Baeza, Laurine Lacerda Pigosso, Kleber Santiago Freitas e Silva, Maristela Pereira, Marcos Antonio Batista de Carvalho Júnior and Célia Maria de Almeida Soares
J. Fungi 2023, 9(11), 1088; https://doi.org/10.3390/jof9111088 - 7 Nov 2023
Viewed by 1608
Abstract
The fungal pathogen Paracoccidioides lutzii causes systemic mycosis Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin America. Upon infection, the fungus undergoes a morphological transition to yeast cells and provokes an inflammatory granulomatous reaction with a high number of neutrophils in the [...] Read more.
The fungal pathogen Paracoccidioides lutzii causes systemic mycosis Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin America. Upon infection, the fungus undergoes a morphological transition to yeast cells and provokes an inflammatory granulomatous reaction with a high number of neutrophils in the lungs. In this work, we employed proteomic analysis to investigate the in vitro response of the fungus to the interaction with human neutrophils. Proteomic profiling of P. lutzii yeast cells harvested at 2 and 4 h post interaction with human polymorphonuclear cells allowed the identification of 505 proteins differentially accumulated. The data indicated that P. lutzii yeast cells underwent a shift in metabolism from glycolysis to Beta oxidation, increasing enzymes of the glyoxylate cycle and upregulating enzymes related to the detoxification of oxidative and heat shock stress. To our knowledge, this is the first study employing proteomic analysis in the investigation of the response of a member of the Paracoccidioides genus to the interaction with neutrophils. Full article
(This article belongs to the Special Issue Fungal-Related Proteomics in Biotechnology and Health)
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