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Marine Drugs
Special Issues
Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides
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Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides

A special issue of Marine Drugs (ISSN 1660-3397). This special issue belongs to the section "Structural Studies on Marine Natural Products".

Deadline for manuscript submissions: closed (31 July 2024) | Viewed by 12774

Special Issue Editors

Dr. Lijun You

E-Mail Website
Guest Editor
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Interests: polysaccharides; hypoglycemic; anticancer; antioxidant; anti-UV radiation activities; functional food
Special Issues, Collections and Topics in MDPI journals
Dr. Saiyi Zhong

E-Mail Website
Guest Editor
Department of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China
Interests: food-derive mutagens and carcinogens; anticancer food factors; bioactive chemopreventive food components
Special Issues, Collections and Topics in MDPI journals
Dr. Riming Huang

E-Mail Website
Guest Editor
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, China
Interests: green directional creation and precise nutrition of marine biological polysaccharides/oligosaccharides

Special Issue Information

Dear Colleagues,                

We are pleased to invite you to submit original articles to the Special Issue entitled “Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides”. The purpose of this Special Issue is to feature latest, high-quality, advanced research and knowledge contributed by various research groups all around the world working on polysaccharides and peptides from marine sources, which will shape future research directions.

Polysaccharides and peptides play a vital role in the areas of human wellness and food. They have been reported to present various physiological and biological activities based on their specific chemical structures. However, their structure–function relationships are yet to be established. Today, special attention has been paid to investigating their structure–function relationships and the mechanisms of their biological activities, which facilitate their being used as food ingredients or bioactive compounds in the food and pharmaceutical fields.

We would like to invite scientists to participate in this Special Issue by submitting an updated and new base of knowledge on the isolation, identification and applications of marine source polysaccharides and peptides.

Potential topics include, but are not limited to, the following:

  • Isolation and structural identification of marine-source polysaccharides or peptides
  • Effects of processing methods on the functions of marine source polysaccharides or peptides.
  • Functions of marine-source polysaccharides or peptides, in vitro or in vivo evaluation
  • Industrial and potential applications of marine-source polysaccharides or peptides.
  • Product research and development of marine-source polysaccharides or peptides.

Prof. Dr. Lijun You
Dr. Saiyi Zhong
Dr. Riming Huang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • isolation
  • structure
  • polysaccharide
  • peptide
  • identification
  • biological activity

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (6 papers)

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Research

19 pages, 6974 KiB  
Article
Purification of an Acidic Polysaccharide with Anticoagulant Activity from the Marine Sponge Sarcotragus spinosulus
by Gabriele Nieddu, Gabriele Obino, Cristina Ciampelli, Antonio Brunetti, Tiziana Cubeddu, Renata Manconi, Giacinta Angela Stocchino, Giovanni Andrea Deiana, Marilena Formato and Antonio Junior Lepedda
Mar. Drugs 2024, 22(3), 139; https://doi.org/10.3390/md22030139 - 21 Mar 2024
Viewed by 1860
Abstract
Thromboembolic conditions are the most common cause of death in developed countries. Anticoagulant therapy is the treatment of choice, and heparinoids and warfarin are the most adopted drugs. Sulphated polysaccharides extracted from marine organisms have been demonstrated to be effective alternatives, blocking thrombus [...] Read more.
Thromboembolic conditions are the most common cause of death in developed countries. Anticoagulant therapy is the treatment of choice, and heparinoids and warfarin are the most adopted drugs. Sulphated polysaccharides extracted from marine organisms have been demonstrated to be effective alternatives, blocking thrombus formation by inhibiting some factors involved in the coagulation cascade. In this study, four acidic glycan fractions from the marine sponge Sarcotragus spinosulus were purified by anion-exchange chromatography, and their anticoagulant properties were investigated through APTT and PT assays and compared with both standard glycosaminoglycans and holothurian sulphated polysaccharides. Moreover, their topographic localization was assessed through histological analysis, and their cytocompatibility was tested on a human fibroblast cell line. A positive correlation between the amount of acid glycans and the inhibitory effect towards both the intrinsic and extrinsic coagulation pathways was observed. The most effective anticoagulant activity was shown by a highly charged fraction, which accounted for almost half (about 40%) of the total hexuronate-containing polysaccharides. Its preliminary structural characterization, performed through infrared spectroscopy and nuclear magnetic resonance, suggested that it may consist of a fucosylated chondroitin sulphate, whose unique structure may be responsible for the anticoagulant activity reported herein for the first time. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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11 pages, 2493 KiB  
Article
UV/H2O2-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity
by Shiyuan Chang, Xiaoyong Chen, Yifan Chen, Lijun You and Kseniya Hileuskaya
Mar. Drugs 2023, 21(11), 561; https://doi.org/10.3390/md21110561 - 26 Oct 2023
Cited by 5 | Viewed by 1742
Abstract
The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H2O2-degraded polysaccharides from Sargassum fusiforme. Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 [...] Read more.
The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H2O2-degraded polysaccharides from Sargassum fusiforme. Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fucp, t-Manp, 6-Galp, t-Fucp, and 3,4-GlcAp. These results would provide theoretical support for studying the structural properties and biological activities of UV/H2O2-degraded polysaccharides. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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14 pages, 1917 KiB  
Article
Preparation and Characterization of an Oyster Peptide–Zinc Complex and Its Antiproliferative Activity on HepG2 Cells
by Bo Peng, Zhu Chen and Yejia Wang
Mar. Drugs 2023, 21(10), 542; https://doi.org/10.3390/md21100542 - 18 Oct 2023
Cited by 1 | Viewed by 1792
Abstract
It is evident that zinc supplementation is essential for maintaining good health and preventing disease. In this study, a novel oyster peptide–zinc complex with an average molecular weight of 500 Da was prepared from oyster meat and purified using ultrafiltration, ultrasound, a programmed [...] Read more.
It is evident that zinc supplementation is essential for maintaining good health and preventing disease. In this study, a novel oyster peptide–zinc complex with an average molecular weight of 500 Da was prepared from oyster meat and purified using ultrafiltration, ultrasound, a programmed cooling procedure, chelating, and dialysis. The optimal chelating process parameters obtained through a response surface methodology optimization design are a peptide/zinc ratio of 15, pH of 6.53, reaction time of 80 min, and peptide concentration of 0.06 g/mL. Then, the structure of a peptide–zinc complex (named COP2-Zn) was investigated using the UV and infrared spectrums. The results showed that the maximum absorption peak was redshifted from 224.5 nm to 228.3 nm and the main difference of the absorption peaks was 1396.4 cm−1. The cytotoxicity and antiproliferative effects of COP2-Zn were evaluated. The results showed that COP2-Zn had a better antiproliferative effect than the unchelated peptide against HepG2 cells. A DNA flow cytometric analysis showed that COP2-Zn induced S-phase arrest in HepG2 cells in a dose-dependent manner. Additionally, the flow cytometer indicated that COP2-Zn significantly induced HepG2 cell apoptosis. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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12 pages, 3074 KiB  
Article
The Preventive Mechanism of Anserine on Tert-Butyl Hydroperoxide-Induced Liver Injury in L-02 Cells via Regulating the Keap1-Nrf2 and JNK-Caspase-3 Signaling Pathways
by Ming Chen, Jing Luo, Hongwu Ji, Wenkui Song, Di Zhang, Weiming Su and Shucheng Liu
Mar. Drugs 2023, 21(9), 477; https://doi.org/10.3390/md21090477 - 29 Aug 2023
Cited by 1 | Viewed by 1483
Abstract
Anserine is a naturally occurring histidine dipeptide with significant antioxidant activities. This study aimed to investigate the preventive mechanism of anserine on tert-butyl hydroperoxide (TBHP)-induced liver damage in a normal human liver cell line (L-02 cells). The L-02 cells were pretreated with anserine [...] Read more.
Anserine is a naturally occurring histidine dipeptide with significant antioxidant activities. This study aimed to investigate the preventive mechanism of anserine on tert-butyl hydroperoxide (TBHP)-induced liver damage in a normal human liver cell line (L-02 cells). The L-02 cells were pretreated with anserine (10, 20, and 40 mmol/L) and then induced with 400 μmol/L of TBHP for 4 h. The results showed that the survival rates of L-02 cells and the contents of GSH were significantly increased with the pretreatment of anserine; the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the extracellular fluid were sharply decreased; and the formation of reactive oxygen species (ROS), nuclear fragmentation, and apoptosis were significantly inhibited. In addition, anserine could bind to the Kelch domain of Kelch-like ECH-associated protein 1 (Keap1) with a binding force of −7.2 kcal/mol; the protein expressions of nuclear factor-erythroid 2-related factor-2 (Nrf2), quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), and Bcl-2 were upregulated by anserine in TBHP-induced L-02 cells, with the downregulation of p-JNK and caspase-3. In conclusion, anserine might alleviated liver injury in L-02 cells via regulating related proteins in the Keap1-Nrf2 and JNK-Caspase-3 signaling pathways. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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15 pages, 2211 KiB  
Article
Identification, Screening and Antibacterial Mechanism Analysis of Novel Antimicrobial Peptides from Sturgeon (Acipenser ruthenus) Spermary
by Hai-Lan Li, Ya-Nan Chen, Jun Cai, Tao Liao and Xiao-Yan Zu
Mar. Drugs 2023, 21(7), 386; https://doi.org/10.3390/md21070386 - 29 Jun 2023
Cited by 11 | Viewed by 2280
Abstract
Fish is an important source of antimicrobial peptides. This study aimed to identify and screen antibacterial peptides with excellent antibacterial activity derived from sturgeon spermary peptides (SSPs) and to analyze their antibacterial activity and mechanism. Liquid chromatography-mass spectrometry/mass spectrometry methods were used to [...] Read more.
Fish is an important source of antimicrobial peptides. This study aimed to identify and screen antibacterial peptides with excellent antibacterial activity derived from sturgeon spermary peptides (SSPs) and to analyze their antibacterial activity and mechanism. Liquid chromatography-mass spectrometry/mass spectrometry methods were used to analyze and identify peptide sequences, computational prediction tool and molecular docking methods were used for virtual screening of antimicrobial peptides, and finally, candidate peptides were synthesized by solid-phase synthesis method. The results demonstrate that SSPs have excellent inhibitory activity against Escherichia coli with an inhibitory rate of 76.46%. Most parts of the SSPs were derived from the sturgeon (Acipenser ruthenus) histones, and the coverage of histone H2B was the highest (45%). Two novel peptides (NDEELNKLM and RSSKRRQ) were obtained by in silico prediction tools and molecular docking, which may interact with the DNA gyrase and dihydrofolate reductase of E. coli by forming salt bridges and hydrogen bonds. Compared to the individual peptides, the antibacterial effect was significantly improved by mixing the two peptides in equal proportions. Two novel peptides change the permeability of the E. coli cell membranes and may exert antimicrobial activity by inhibiting the metabolic process of the nucleic acids. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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12 pages, 2974 KiB  
Article
Extraction, Characterization and Osteogenic Activity of a Type I Collagen from Starfish (Asterias amurensis)
by Lingcui Li, Yu Yu, Wenhui Wu and Peipei Wang
Mar. Drugs 2023, 21(5), 274; https://doi.org/10.3390/md21050274 - 27 Apr 2023
Cited by 7 | Viewed by 2684
Abstract
Outbreaks of starfish (Asterias amurensis) pose a major threat to aquaculture and marine ecosystems in Qingdao, China, and no effective methods have been found to control them. A comprehensive study of collagen in starfish could be an alternative to high efficient [...] Read more.
Outbreaks of starfish (Asterias amurensis) pose a major threat to aquaculture and marine ecosystems in Qingdao, China, and no effective methods have been found to control them. A comprehensive study of collagen in starfish could be an alternative to high efficient utilization. Based on this, collagen was firstly extracted from Qingdao A. amurensis. Then, its protein pattern, amino acid composition, secondary structure, microstructure and thermal stability were investigated. The results showed that the A. amurensis collagen (AAC) is a type I collagen composed of α1, α2, and β chains. Glycine, hydroxyproline, and alanine were the major amino acids. The melting temperature was 57.7 °C. From FTIR, UV spectra and CD chromatography, the AAC had an intact triple helix and secondary structure, and microstructural analysis showed that the AAC had a loose, fibrous porous structure. Next, the osteogenic differentiation effect of AAC on Mouse bone marrow stem cells (BMSCs) was investigated, and the results showed that AAC induced osteogenic differentiation of cells by promoting the proliferation of BMSCs, enhancing alkaline phosphatase (ALP) activity, promoting cell mineralization nodules and upregulating the expression of mRNA of relevant osteogenic genes. These results suggest that AAC might have the potential application to bone health-related functional foods. Full article
(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)
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