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Metabolites
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LC-MS/MS Analysis for Plant Secondary Metabolites
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LC-MS/MS Analysis for Plant Secondary Metabolites

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Plant Metabolism".

Deadline for manuscript submissions: closed (20 September 2024) | Viewed by 3884

Special Issue Editors

Dr. Tiantian Ye

E-Mail Website
Guest Editor
Department of Chemistry, Wuhan University, Wuhan, China
Interests: LC-MS analysis; metabolomics; nucleic acid modifications
Dr. Lin Li

E-Mail Website
Guest Editor
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China
Interests: proteomics; metabolomics; multi-omics; cancer research; precision medicine; interaction networks

Special Issue Information

Dear Colleagues,

Plant secondary metabolites encompass a diverse group of molecules that play a critical role, yet they are not directly involved in plant growth, development, or stress response. Accurate and reliable analysis of the secondary metabolites would enhance our comprehension of their physiological status and deepen our understanding of specific biochemical processes in plants. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and essential technique for metabolomics analysis. Advancements in LC-MS/MS have facilitated the simultaneous measurement of numerous metabolites. Nevertheless, obtaining high-quality metabolomics datasets as well as effectively integrating and interpreting the data still present challenges.

In this Special Issue on "LC-MS/MS Analysis for Plant Secondary Metabolites”, we aim to showcase the wide range of research and applications of LC-MS/MS analysis in the field of plant secondary metabolites. We invite submissions covering various aspects of LC-MS/MS analysis, including applications, method and instrumentation development, and advancements in bioinformatic methodology. Original research papers as well as review articles are welcomed for submission.

Dr. Tiantian Ye
Dr. Lin Li
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid chromatography
  • mass spectrometry
  • secondary metabolites
  • metabolomics
  • abiotic and biotic stress
  • plant development

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Published Papers (3 papers)

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Research

15 pages, 9123 KiB  
Article
Comparative Analysis of the Chemical Constituents of Chrysanthemum morifolium with Different Drying Processes Integrating LC/GC–MS−Based, Non-Targeted Metabolomics
by Na Chen, Jizhou Fan, Gang Li, Xuanxuan Guo, Xiao Meng, Yuqing Wang, Yingying Duan, Wanyue Ding, Kai Liu, Yaowu Liu and Shihai Xing
Metabolites 2024, 14(9), 481; https://doi.org/10.3390/metabo14090481 - 2 Sep 2024
Viewed by 992
Abstract
Chrysanthemum morifolium is a perennial herbaceous plant in the Asteraceae family that is used as a medicine and food owing to its superior pharmacological properties. Irrespective of its application, C. morifolium must be dried before use. Shade drying (YG) and heat drying (HG) [...] Read more.
Chrysanthemum morifolium is a perennial herbaceous plant in the Asteraceae family that is used as a medicine and food owing to its superior pharmacological properties. Irrespective of its application, C. morifolium must be dried before use. Shade drying (YG) and heat drying (HG) are the two drying methods used in most origins. Given the abundance of flavonoids, phenolic acids, and terpenoids, the primary medicinal active constituents of C. morifolium, it is important to determine whether the composition and content of these compounds are altered during the drying processes. To test this, the changes in the chemical composition of C. morifolium flowers after YG and HG using full-spectrum, non-targeted LC/GC–MS−based metabolomics and, subsequently, the three indicator components of C. morifolium—chlorogenic acid, 3,5−dicaffeoylquinic acid, and luteolin−7−O−glucoside—were accurately quantified by HPLC. The results of the non-targeted metabolomics analysis revealed that YG- and HG-processed C. morifolium differed significantly with respect to chemical contents, especially flavonoids, phenolic acids, and terpenoids. The levels of the indicator components and their precursors also differed significantly between the YG and HG treatments. The contents of most of the flavonoids and key phenolic acids, terpenoids, and carbohydrates were higher with YG than with HG pre-treatment. These results revealed the changes in the chemical composition of C. morifolium during the YG and HG processes, thus providing a reference for the further optimization of the production and processing of chrysanthemums. Full article
(This article belongs to the Special Issue LC-MS/MS Analysis for Plant Secondary Metabolites)
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14 pages, 4826 KiB  
Article
Study on Synthesis and Regulation of PPVI and PPVII in Paris polyphylla with UV
by Dongjie Geng, Yiqun Sun, Shouzan Liu, Wen Chen, Fei Gao, Yan Bai and Shaobo Zhang
Metabolites 2024, 14(8), 427; https://doi.org/10.3390/metabo14080427 - 2 Aug 2024
Viewed by 961
Abstract
Paris polyphylla Smith var. Chinensis (Franch.) Hara is a medicinal plant that belongs to the Liliaceae family. Its main components are parissaponins, which have excellent medicinal effects such as anti-inflammatory, anti-tumor, etc. Improving the quality of parissaponins through artificial directional regulation has emerged [...] Read more.
Paris polyphylla Smith var. Chinensis (Franch.) Hara is a medicinal plant that belongs to the Liliaceae family. Its main components are parissaponins, which have excellent medicinal effects such as anti-inflammatory, anti-tumor, etc. Improving the quality of parissaponins through artificial directional regulation has emerged as a practice to meet medical demand and is a new research hotspot. In this paper, P. polyphylla plants were treated with UVA, UVB, and UVC, and the contents of PolyPhyllin VI (PPVI) and PolyPhyllin VII (PPVII), saponin synthase (squalene synthase, SS; cycloartenol synthase, CAS; cytochrome P450, CYP450; and glycosyl transferases, GT) activity, MDA, and the photosynthetic pigment indexes were measured and analyzed. The results showed that PPVII content increased by 32.43% with UVC treatment after 4 h (3.43 mg/g), but the PPVI and PPVII contents in the other groups decreased compared with CK (control group) and they did not return to the original level after 4 h. SS, CAS, CYP450, and GT synthases were activated in varying degrees via UV treatment and increased, respectively, by 22.93%, 10.83%, 20.15%, and 25.98%. Among them, GT, as the last of the synthetases, had a shorter response time to UVB (30 min) and UVC (15 min); the difference was sensible compared with CK. Moreover, UV had a stressing effect and promoted the rapid accumulation of MDA content (increased 17.66%, 34.53%, and 9.65%) and carotenoid (increased 7.58, 5.60, and 7.76 times) within 4 h compared to CK. UVB and UVC radiation visibly improved chlorophyll a content (42.56% and 35.45%), but UVA did not, and the change in chlorophyll b content showed no overt statistical difference. In addition, PPVI and PPVII were negatively correlated with SS, CAS, carotenoids, and MDA (p < 0.05) and positively correlated with CYP450, GT, and chlorophyll a (p < 0.05). This study provides a theoretical basis for using UV light to regulate secondary metabolism in P. polyphylla, which is of great value for production management. Full article
(This article belongs to the Special Issue LC-MS/MS Analysis for Plant Secondary Metabolites)
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21 pages, 1326 KiB  
Article
Metabolite Profiling Analysis of the Tongmai Sini Decoction in Rats after Oral Administration through UHPLC-Q-Exactive-MS/MS
by Xianhui Zheng, Yingying Zhan, Mengling Peng, Wen Xu and Guanghai Deng
Metabolites 2024, 14(6), 333; https://doi.org/10.3390/metabo14060333 - 14 Jun 2024
Cited by 1 | Viewed by 1123
Abstract
Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still [...] Read more.
Tongmai Sini decoction (TSD), the classical prescriptions of traditional Chinese medicine, consisting of three commonly used herbal medicines, has been widely applied for the treatment of myocardial infarction and heart failure. However, the absorbed components and their metabolism in vivo of TSD still remain unknown. In this study, a reliable and effective method using ultra-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive-MS/MS) was employed to identify prototype components and metabolites in vivo (rat plasma and urine). Combined with mass defect filtering (MDF), dynamic background subtraction (DBS), and neutral loss filtering (NLF) data-mining tools, a total of thirty-two major compounds were selected and investigated for their metabolism in vivo. As a result, a total of 82 prototype compounds were identified or tentatively characterized in vivo, including 41 alkaloids, 35 phenolic compounds, 6 saponins. Meanwhile, A total of 65 metabolites (40 alkaloids and 25 phenolic compounds) were tentatively identified. The metabolic reactions were mainly hydrogenation, demethylation, hydroxylation, hydration, methylation, deoxylation, and sulfation. These findings will be beneficial for an in-depth understanding of the pharmacological mechanism and pharmacodynamic substance basis of TSD. Full article
(This article belongs to the Special Issue LC-MS/MS Analysis for Plant Secondary Metabolites)
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