Integration of Emerging Technologies in Metabolite Analysis

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Metabolomic Profiling Technology".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 931

Special Issue Editor


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Guest Editor
Department of Chemistry—Biomedical Center (BMC), Box 599, Uppsala University, 751 24 Uppsala, Sweden
Interests: gas chromatography; liquid chromatography; mass spectrometry; metabolomics; NMR; smaller molecules; supercritical chromatography

Special Issue Information

Dear Colleagues,

In recent years, the field of metabolomics has witnessed rapid developments powered by the integration of novel technologies. This Special Issue aims to explore the application of emerging technologies in metabolite analysis, shedding light on their potential to revolutionize our understanding of metabolic processes and their implications for human health and disease.

The contributions to this Special Issue include a diverse array of methodologies and approaches, from mass spectrometry and nuclear magnetic resonance spectroscopy with chromatographic techniques. These technologies offer unique sensitivity, resolution, and throughput, enabling comprehensive profiling of metabolites in complex biological systems.

Furthermore, this Special Issue highlights the innovative strategies developed for metabolite identification, quantification, and data analysis, including metabolic pathway modeling, and multi-omics integration. By connecting the power of these cutting-edge technologies, researchers can unravel complex metabolic networks, uncover biomarkers of disease, and accelerate the discovery of therapeutic targets.

Moreover, the articles featured in this Special Issue explore the application of metabolomics in various research domains, such as cancer biology, drug discovery, environmental science, and personalized medicine. By elucidating the dynamic interplay between metabolites and physiological processes, these studies cover the way for the development of precision diagnostics and tailored interventions for metabolic disorders.

In conclusion, this Special Issue provides a comprehensive overview of the latest advancements in metabolite analysis, highlighting the transformative impact of emerging technologies on the field of metabolomics. Through collaborative efforts and interdisciplinary approaches, researchers can attach these innovations to address fundamental questions in biology and medicine, ultimately improving human health and well-being.

Dr. Kumari Ubhayasekera
Guest Editor

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Keywords

  • liquid chromatography
  • mass spectrometry
  • NMR
  • metabolomics
  • smaller molecules
  • supercritical chromatography
  • gas chromatography

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Published Papers (1 paper)

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14 pages, 1907 KiB  
Article
Online Direct Infusion Mass Spectrometry of Liquid–Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe
by Cátia Marques, Lena Blaase and Ingela Lanekoff
Metabolites 2024, 14(11), 587; https://doi.org/10.3390/metabo14110587 - 30 Oct 2024
Viewed by 644
Abstract
Background/Objectives: Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both metabolites and lipids in the same sample can enhance these understandings and connect cellular dynamics. However, simultaneous detection of metabolites and [...] Read more.
Background/Objectives: Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both metabolites and lipids in the same sample can enhance these understandings and connect cellular dynamics. However, simultaneous detection of metabolites and lipids is generally hampered by chromatographic systems tailored to one molecular type. This void can be filled by direct infusion mass spectrometry (MS), where all ionizable molecules can be detected simultaneously. However, in direct infusion MS, the high chemical complexity of biological samples can introduce limitations in detectability due to matrix effects causing ionization suppression. Methods: Decreased sample complexity and increased detectability and molecular coverage was provided by combining our direct infusion probe (DIP) with liquid–liquid extraction (LLE) and directly sampling the different phases for direct infusion. Three commonly used LLE methods for separating lipids and metabolites were evaluated. Results: The butanol–methanol (BUME) method was found to be preferred since it provides high molecular coverage and have low solvent toxicity. The established BUME DIP-MS method was used as a fast and sensitive analysis tool to study chemical changes in insulin-secreting cells upon glucose stimulation. By analyzing the metabolome at distinct time points, down to 1-min apart, we found high dynamics of the intracellular metabolome. Conclusions: The rapid workflow with LLE DIP-MS enables higher sensitivity of phase separated metabolites and lipids. The application of BUME DIP-MS provides novel information on the dynamics of the intracellular metabolome of INS-1 during the two phases of insulin release for both metabolite and lipid classes. Full article
(This article belongs to the Special Issue Integration of Emerging Technologies in Metabolite Analysis)
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