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Micromachines
Special Issues
Biomaterials and Biodevices for Rapid Detection
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Biomaterials and Biodevices for Rapid Detection

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B：Biology and Biomedicine".

Deadline for manuscript submissions: closed (30 May 2024) | Viewed by 4442

Special Issue Editor

Prof. Dr. Van Thuan Nguyen

E-Mail Website
Guest Editor
Department of Biology, Western Kentucky University, Bowling Green, KY 42101, USA
Interests: aptamer; aptasensor; lateral flow cartridges; wearable biosensors; emerging biosensors; POC biosensors; nanobiosensors; DNA chips; microarray; gene expression; cancer cell therapeutics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Biosensors, especially Point-of-care (POC) biosensors, have grown rapidly and are recognized as potentially powerful analytical devices for in-field medical diagnostics, food safety, environmental monitoring and other emerging applications. Diverse technologies, platforms, and new biomaterials have been utilized for high-sensitivity rapid detection biosensors and commercialization efforts are ongoing. With advances in high-sensitivity POC detection technologies, wearable-portable biodevices, new physical and chemical transducers, new biomaterials along with chemical recognition/amplification strategies, there are lots of opportunities for rapid detection biosensors. This special issue seeks to focus on biosensors-relevant rapid detection, early medical diagnostics, and environmental monitoring, including, but not limited to, POC biosensors, emerging biosensors, lateral flow assays, microfluidic biosensors, diagnostic pathogenic diseases, ultra-sensitive technologies, rapid test kits, non-invasive biosensors, smartphone biosensors and new biomaterials applied to rapid detection. Both original research articles and reviews are welcomed.

Prof. Dr. Van Thuan Nguyen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • POC biosensors (electrochemical, optical, magnetic-based, thermal-based)
  • ultra-sensitive technologies
  • rapid detection biosensors (electrochemical, optical, magnetic-based)
  • biomaterials for Rapid Detection
  • lateral flow assays (LFA)
  • microfluidic platforms
  • smartphone technologies
  • rapid test kit
  • environmental monitoring biosensors
  • emerging biosensors

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Published Papers (2 papers)

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Research

26 pages, 2964 KiB  
Article
Bioaffinity Nanoprobes for Foodborne Pathogen Sensing
by Tracy Ann Bruce-Tagoe and Michael K. Danquah
Micromachines 2023, 14(6), 1122; https://doi.org/10.3390/mi14061122 - 26 May 2023
Cited by 4 | Viewed by 1574
Abstract
Bioaffinity nanoprobes are a type of biosensor that utilize the specific binding properties of biological molecules, such as antibodies, enzymes, and nucleic acids, for the detection of foodborne pathogens. These probes serve as nanosensors and can provide highly specific and sensitive detection of [...] Read more.
Bioaffinity nanoprobes are a type of biosensor that utilize the specific binding properties of biological molecules, such as antibodies, enzymes, and nucleic acids, for the detection of foodborne pathogens. These probes serve as nanosensors and can provide highly specific and sensitive detection of pathogens in food samples, making them an attractive option for food safety testing. The advantages of bioaffinity nanoprobes include their ability to detect low levels of pathogens, rapid analysis time, and cost-effectiveness. However, limitations include the need for specialized equipment and the potential for cross-reactivity with other biological molecules. Current research efforts focus on optimizing the performance of bioaffinity probes and expanding their application in the food industry. This article discusses relevant analytical methods, such as surface plasmon resonance (SPR) analysis, Fluorescence Resonance Energy Transfer (FRET) measurements, circular dichroism, and flow cytometry, that are used to evaluate the efficacy of bioaffinity nanoprobes. Additionally, it discusses advances in the development and application of biosensors in monitoring foodborne pathogens. Full article
(This article belongs to the Special Issue Biomaterials and Biodevices for Rapid Detection)
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14 pages, 3004 KiB  
Article
High-Throughput Gel Microbeads as Incubators for Bacterial Competition Study
by Trang Anh Nguyen-Le, Xinne Zhao, Michael Bachmann, Philip Ruelens, J. Arjan G. M. de Visser and Larysa Baraban
Micromachines 2023, 14(3), 645; https://doi.org/10.3390/mi14030645 - 12 Mar 2023
Cited by 2 | Viewed by 2392
Abstract
Bacteria primarily live in structured environments, such as colonies and biofilms, attached to surfaces or growing within soft tissues. They are engaged in local competitive and cooperative interactions impacting our health and well-being, for example, by affecting population-level drug resistance. Our knowledge of [...] Read more.
Bacteria primarily live in structured environments, such as colonies and biofilms, attached to surfaces or growing within soft tissues. They are engaged in local competitive and cooperative interactions impacting our health and well-being, for example, by affecting population-level drug resistance. Our knowledge of bacterial competition and cooperation within soft matrices is incomplete, partly because we lack high-throughput tools to quantitatively study their interactions. Here, we introduce a method to generate a large amount of agarose microbeads that mimic the natural culture conditions experienced by bacteria to co-encapsulate two strains of fluorescence-labeled Escherichia coli. Focusing specifically on low bacterial inoculum (1–100 cells/capsule), we demonstrate a study on the formation of colonies of both strains within these 3D scaffolds and follow their growth kinetics and interaction using fluorescence microscopy in highly replicated experiments. We confirmed that the average final colony size is inversely proportional to the inoculum size in this semi-solid environment as a result of limited available resources. Furthermore, the colony shape and fluorescence intensity per colony are distinctly different in monoculture and co-culture. The experimental observations in mono- and co-culture are compared with predictions from a simple growth model. We suggest that our high throughput and small footprint microbead system is an excellent platform for future investigation of competitive and cooperative interactions in bacterial communities under diverse conditions, including antibiotics stress. Full article
(This article belongs to the Special Issue Biomaterials and Biodevices for Rapid Detection)
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