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Journals
Microorganisms
Special Issues
Biotechnology of Cold-Adapted Bacteria and Marine Bacteria
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Biotechnology of Cold-Adapted Bacteria and Marine Bacteria

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (20 September 2021) | Viewed by 17640

Special Issue Editors

Dr. Maria Luisa Tutino

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Guest Editor
Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy
Interests: Pseudoalteromonas haloplanktis genetics and genomics; psychrophilic bacteria fermentation; psychrophilic bacteria physiology; bioactive compounds from marine bacteria; recombinant protein production in Pseudoalteromonas haloplanktis
Special Issues, Collections and Topics in MDPI journals
Dr. Andrea Colarusso

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Guest Editor
Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy
Interests: biotechnology; recombinant protein production; modelling of biological processes and of production pipelines; strain characterization and engineering; genomics and transcriptomics; drug design

Special Issue Information

Dear Colleagues,

Permanent or transient cold environments are prevalent on our planet. Indeed, besides the Polar and Alpine areas, a large portion of seawater has a temperature below 15 °C. We are just approaching the study of the microbiome that thrives in these habitats, uncovering a “world of opportunities” for biotechnologists.

This Special Issue aims to report the cutting-edge applications of microorganisms isolated from sea and/or from cold environments, their enzymes and their chemical diversity in different fields of modern biotechnologies, such as (and not limited to):

  • Food applications
  • Detergents and the cleaning industry
  • Environmental biotechnologies (wastewater treatments and bioremediation)
  • Biofuels and energy production
  • Molecular, structural biology and recombinant protein production
  • Pharmaceutical and medical industry

Dr. Maria Luisa Tutino
Dr. Andrea Colarusso
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cold-adapted enzyme
  • cold-adapted bacteria
  • marine bacteria
  • white biotechnologies
  • enzyme catalysis
  • microbial catalysis
  • food biotechnologies
  • environmental biotechnologies
  • detergent industry
  • cleaning industry
  • biofuels
  • molecular biology
  • recombinant protein production
  • pharmaceutical industry
  • structural biology

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Published Papers (4 papers)

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Research

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12 pages, 1964 KiB  
Article
Low-Temperature Biodegradation of Lignin-Derived Aromatic Model Monomers by the Cold-Adapted Yeast Rhodosporidiobolus colostri Isolated from Alpine Forest Soil
by Rosa Margesin, Thomas Marek Ludwikowski, Andrea Kutzner and Andreas Otto Wagner
Microorganisms 2022, 10(3), 515; https://doi.org/10.3390/microorganisms10030515 - 26 Feb 2022
Cited by 8 | Viewed by 2338
Abstract
The contribution of cold-adapted yeasts to the emerging field of lignin biovalorization has not yet been studied. The red-pigmented basidiomycetous yeast strain Rhodosporidiobolus colostri DBVPG 10655 was examined for its potential to degrade five selected lignin-derived aromatic monomers (syringic acid, p-coumaric [...] Read more.
The contribution of cold-adapted yeasts to the emerging field of lignin biovalorization has not yet been studied. The red-pigmented basidiomycetous yeast strain Rhodosporidiobolus colostri DBVPG 10655 was examined for its potential to degrade five selected lignin-derived aromatic monomers (syringic acid, p-coumaric acid, 4-hydroxybenzoic acid, ferulic acid, and vanillic acid). The strain utilized p-coumaric acid, 4-hydroxybenzoic acid, and ferulic acid not only as the sole carbon source; full biodegradation occurred also in mixtures of multiple monomers. Vanillic acid was not utilized as the sole carbon source, but was degraded in the presence of p-coumaric acid, 4-hydroxybenzoic acid, and ferulic acid. Syringic acid was utilized neither as the sole carbon source nor in mixtures of compounds. Biodegradation of lignin-derived aromatic monomers was detected over a broad temperature range (1–25 °C), which is of ecological significance and of biotechnological relevance. Full article
(This article belongs to the Special Issue Biotechnology of Cold-Adapted Bacteria and Marine Bacteria)
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14 pages, 2134 KiB  
Article
Cryopreservation of Cyanobacteria and Eukaryotic Microalgae Using Exopolysaccharide Extracted from a Glacier Bacterium
by Pervaiz Ali, Daniel Fucich, Aamer Ali Shah, Fariha Hasan and Feng Chen
Microorganisms 2021, 9(2), 395; https://doi.org/10.3390/microorganisms9020395 - 15 Feb 2021
Cited by 14 | Viewed by 4449
Abstract
Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of [...] Read more.
Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of three unicellular cyanobacteria and two eukaryotic microalgae. Different concentrations of EPS (10%, 15%, and 20%) were tested, and the highest concentration (20%) of EPS yielded the best growth recovery for the algal strains we tested. We also compared EPS with 5% dimethyl sulfoxide (DMSO) and 10% glycerol for the cryopreservation recovery. The growth recovery for the microalgal strains after nine months of cryopreservation was better than 5% DMSO, a well-known cryoprotectant for microalgae. A poor recovery was recorded for all the tested strains with 10% glycerol as a cryoprotective agent. The patterns of growth recovery for most of these strains were similar after 5 days, 15 days, and 9 months of cryopreservation. Unlike common cryopreservants such as DMSO or methanol, which are hazardous materials, EPS is safe to handle. We demonstrate that the EPS from a psychrotrophic bacterium helped in the long-term cryopreservation of cyanobacteria and microalgae, and it has the potential to be used as natural cryoprotective agent for other cells. Full article
(This article belongs to the Special Issue Biotechnology of Cold-Adapted Bacteria and Marine Bacteria)
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24 pages, 6631 KiB  
Article
Improvement of Pseudoalteromonas haloplanktis TAC125 as a Cell Factory: IPTG-Inducible Plasmid Construction and Strain Engineering
by Andrea Colarusso, Concetta Lauro, Marzia Calvanese, Ermenegilda Parrilli and Maria Luisa Tutino
Microorganisms 2020, 8(10), 1466; https://doi.org/10.3390/microorganisms8101466 - 24 Sep 2020
Cited by 8 | Viewed by 3214
Abstract
Our group has used the marine bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125) as a platform for the successful recombinant production of “difficult” proteins, including eukaryotic proteins, at low temperatures. However, there is still room for improvement both in the refinement of Ph [...] Read more.
Our group has used the marine bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125) as a platform for the successful recombinant production of “difficult” proteins, including eukaryotic proteins, at low temperatures. However, there is still room for improvement both in the refinement of PhTAC125 expression plasmids and in the bacterium’s intrinsic ability to accumulate and handle heterologous products. Here, we present an integrated approach of plasmid design and strain engineering finalized to increment the recombinant expression and optimize the inducer uptake in PhTAC125. To this aim, we developed the IPTG-inducible plasmid pP79 and an engineered PhTAC125 strain called KrPL LacY+. This mutant was designed to express the E. coli lactose permease and to produce only a truncated version of the endogenous Lon protease through an integration-deletion strategy. In the wild-type strain, pP79 assured a significantly better production of two reporters in comparison to the most recent expression vector employed in PhTAC125. Nevertheless, the use of KrPL LacY+ was crucial to achieving satisfying production levels using reasonable IPTG concentrations, even at 0 °C. Both the wild-type and the mutant recombinant strains are characterized by an average graded response upon IPTG induction and they will find different future applications depending on the desired levels of expression. Full article
(This article belongs to the Special Issue Biotechnology of Cold-Adapted Bacteria and Marine Bacteria)
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Review

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26 pages, 36876 KiB  
Review
Oil Bioremediation in the Marine Environment of Antarctica: A Review and Bibliometric Keyword Cluster Analysis
by Nur Nadhirah Zakaria, Peter Convey, Claudio Gomez-Fuentes, Azham Zulkharnain, Suriana Sabri, Noor Azmi Shaharuddin and Siti Aqlima Ahmad
Microorganisms 2021, 9(2), 419; https://doi.org/10.3390/microorganisms9020419 - 17 Feb 2021
Cited by 24 | Viewed by 6617
Abstract
Bioremediation of hydrocarbons has received much attention in recent decades, particularly relating to fuel and other oils. While of great relevance globally, there has recently been increasing interest in hydrocarbon bioremediation in the marine environments of Antarctica. To provide an objective assessment of [...] Read more.
Bioremediation of hydrocarbons has received much attention in recent decades, particularly relating to fuel and other oils. While of great relevance globally, there has recently been increasing interest in hydrocarbon bioremediation in the marine environments of Antarctica. To provide an objective assessment of the research interest in this field we used VOSviewer software to analyze publication data obtained from the ScienceDirect database covering the period 1970 to the present, but with a primary focus on the years 2000–2020. A bibliometric analysis of the database allowed identification of the co-occurrence of keywords. There was an increasing trend over time for publications relating to oil bioremediation in maritime Antarctica, including both studies on marine bioremediation and of the metabolic pathways of hydrocarbon degradation. Studies of marine anaerobic degradation remain under-represented compared to those of aerobic degradation. Emerging keywords in recent years included bioprospecting, metagenomic, bioindicator, and giving insight into changing research foci, such as increasing attention to microbial diversity. The study of microbial genomes using metagenomic approaches or whole genome studies is increasing rapidly and is likely to drive emerging fields in future, including rapid expansion of bioprospecting in diverse fields of biotechnology. Full article
(This article belongs to the Special Issue Biotechnology of Cold-Adapted Bacteria and Marine Bacteria)
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