Recent Advances in Coxsackievirus Infections: Epidemiology, Pathogenesis and Methods for Control and Prevention

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: closed (30 May 2024) | Viewed by 2900

Special Issue Editors


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Guest Editor
Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia
Interests: coxsackieviruses; epidemiology; pathogenesis; virulence; vaccines

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Special Issue Information

Dear Colleagues,

Coxsackieviruses (CVs) are ubiquitous and widespread single-stranded RNA viruses belonging to the Picornaviridae family and the genus Enterovirus. These viruses are mainly transmitted via the fecal–oral route and are considered a major cause of viral infection each year worldwide. Coxsackieviruses were first isolated in the late 1940s from the feces of paralyzed children living in Coxsackie (New York, USA) and were classified into CV groups A (CVA) and B (CVB) based on the nature of their pathogenicity in mice. Coxsackievirus infections are considered to be asymptomatic or to induce subclinical or mild symptoms. They are also associated with various acute and chronic pathologies (hand, foot, and mouth disease caused by CVA, chronic myocarditis, dilated cardiomyopathy, and autoimmune diseases such as type 1 diabetes T1D caused by CVB…). This Special Issue encourages researchers worldwide to present their latest research on CV epidemiology, virulence, cellular and molecular mechanisms involved in CV pathogenesis, and the new antiviral strategies to control CV infections.

We look forward to receiving your contributions to this Special Issue in the form of original research articles, review articles, and small reviews, including communications and opinions regarding all aspects of coxsackievirus molecular biology, epidemiology, diagnosis, virulence, pathogenesis, and future strategies to control these viruses including vaccines and antiviral molecules.

Prof. Dr. Jawhar Gharbi
Prof. Dr. Didier Hober
Guest Editors

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Published Papers (1 paper)

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Research

13 pages, 4624 KiB  
Article
Cloning and Molecular Characterization of the Recombinant CVB4E2 Immunogenic Viral Protein (rVP1), as a Potential Subunit Protein for Vaccine and Immunodiagnostic Reagent Candidate
by Ikbel Hadj Hassine, Jawhar Gharbi, Imene Amara, Ameera Alyami, Reem Subei, Mohammed Almalki, Didier Hober and Manel Ben M’hadheb
Microorganisms 2023, 11(5), 1192; https://doi.org/10.3390/microorganisms11051192 - 1 May 2023
Cited by 3 | Viewed by 2184
Abstract
The aim of the present study was, first, to clone the VP1 gene of the human coxsackievirus B4 strain E2 (CVB4E2) in the prokaryotic pUC19 plasmid expression vector then to compare it with the structural capsid proteins of the same strain using bioinformatic [...] Read more.
The aim of the present study was, first, to clone the VP1 gene of the human coxsackievirus B4 strain E2 (CVB4E2) in the prokaryotic pUC19 plasmid expression vector then to compare it with the structural capsid proteins of the same strain using bioinformatic tools. PCR colony amplification followed through a restriction digestion analysis and sequencing process which affirmed the success of the cloning process. SDS-PAGE and Western Blotting were used to characterize the purified recombinant viral protein expressed in bacteria cells. The BLASTN tool revealed that the nucleotide sequence of the recombinant VP1 (rVP1) expressed by pUC19 highly matched the target nucleotide sequence of the diabetogenic CVB4E2 strain. Secondary structure and three-dimension structure prediction suggested that rVP1, such as wild-type VP1, is chiefly composed of random coils and a high percentage of exposed amino acids. Linear B-cell epitope prediction showed that several antigenic epitopes are likely present in rVP1 and CVB4E2 VP1 capsid protein. Additionally, phosphorylation site prediction revealed that both proteins may affect the signal transduction of host cells and can be involved in virus virulence. The present work highlights the usefulness of cloning and bioinformatics characterizations for gene investigation. Furthermore, the collected data are helpful for future experimental research related to the development of immunodiagnostic reagents and subunit vaccines based on the expression of immunogenic viral capsid proteins. Full article
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