Non-Coding RNA and Their Regulatory Roles in Plant

A special issue of Non-Coding RNA (ISSN 2311-553X). This special issue belongs to the section "Computational Biology".

Deadline for manuscript submissions: closed (20 October 2024) | Viewed by 7201

Special Issue Editor


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Guest Editor
Tecnologico de Monterrey, School of Engineering and Sciences, Campus Queretaro, Querétaro 76130, Mexico
Interests: ncRNAs in human diseases; gene regulation; biomarker; therapy; anticancer phytochemicals; plant microRNA; nanotechnology
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Special Issue Information

Dear Colleagues,

Regulatory non-coding RNAs (ncRNAs), which can be short or long ncRNAs (lncRNAs), control a wide range of biological processes in plants. ncRNAs have a variety of forms, including microRNAs (miRNAs), small interfering RNAs (siRNAs), lncRNAs, circular RNAs (circRNAs), and derived ncRNAs, depending on their mode of biogenesis and function. Through interactions with homologous sequences, ncRNAs function as riboregulators to control plants’ development, growth, and stress response signaling. Insights into the roles of non-coding RNAs in plant biology might be of great importance to understand the regulatory pathways used in different plant processes and the development of genetically modified improved crop varieties via ncRNA manipulation.

For this Special Issue, the goal is to solicit submission of any articles to understand the regulatory roles of plant non-coding RNAs and their applications for crop improvement. The subtopics are as follows:

  • The identification and functional characterization of microRNA, lncRNAs, and other ncRNAs in plant.
  • ncRNA-based transgenic and biotechnology.
  • CRISPR-based editing of plant ncRNAs.
  • Computational tools for the functional analysis of plant ncRNAs.
  • Plant ncRNA databases.
  • Understanding the mechanistic roles of ncRNAs in plants’ stress response signaling.
  • Novel techniques, methods, and ideas for analyzing plant ncRNAs.
  • High-throughput sequencing-based analysis of plant ncRNAs.

Prof. Dr. Sujay Paul
Guest Editor

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Keywords

  • plant
  • ncRNAs
  • growth and development
  • stress response signaling
  • transgenic
  • gene regulation

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Published Papers (2 papers)

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19 pages, 3783 KiB  
Article
Analysis of lncRNAs in Lupinus mutabilis (Tarwi) and Their Potential Role in Drought Response
by Manuel Hidalgo, Cynthia Ramos and Gaston Zolla
Non-Coding RNA 2023, 9(5), 48; https://doi.org/10.3390/ncrna9050048 - 23 Aug 2023
Viewed by 2691
Abstract
Lupinus mutabilis is a legume with high agronomic potential and available transcriptomic data for which lncRNAs have not been studied. Therefore, our objective was to identify, characterize, and validate the drought-responsive lncRNAs in L. mutabilis. To achieve this, we used a multilevel [...] Read more.
Lupinus mutabilis is a legume with high agronomic potential and available transcriptomic data for which lncRNAs have not been studied. Therefore, our objective was to identify, characterize, and validate the drought-responsive lncRNAs in L. mutabilis. To achieve this, we used a multilevel approach based on lncRNA prediction, annotation, subcellular location, thermodynamic characterization, structural conservation, and validation. Thus, 590 lncRNAs were identified by at least two algorithms of lncRNA identification. Annotation with the PLncDB database showed 571 lncRNAs unique to tarwi and 19 lncRNAs with homology in 28 botanical families including Solanaceae (19), Fabaceae (17), Brassicaceae (17), Rutaceae (17), Rosaceae (16), and Malvaceae (16), among others. In total, 12 lncRNAs had homology in more than 40 species. A total of 67% of lncRNAs were located in the cytoplasm and 33% in exosomes. Thermodynamic characterization of S03 showed a stable secondary structure with −105.67 kcal/mol. This structure included three regions, with a multibranch loop containing a hairpin with a SECIS-like element. Evaluation of the structural conservation by CROSSalign revealed partial similarities between L. mutabilis (S03) and S. lycopersicum (Solyc04r022210.1). RT-PCR validation demonstrated that S03 was upregulated in a drought-tolerant accession of L. mutabilis. Finally, these results highlighted the importance of lncRNAs in tarwi improvement under drought conditions. Full article
(This article belongs to the Special Issue Non-Coding RNA and Their Regulatory Roles in Plant)
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Review

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25 pages, 846 KiB  
Review
The Emerging Role of Non-Coding RNAs (ncRNAs) in Plant Growth, Development, and Stress Response Signaling
by Amit Yadav, Jyotirmaya Mathan, Arvind Kumar Dubey and Anuradha Singh
Non-Coding RNA 2024, 10(1), 13; https://doi.org/10.3390/ncrna10010013 - 7 Feb 2024
Cited by 10 | Viewed by 3562
Abstract
Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play a crucial regulatory role in plant biology, surpassing the essential functions of RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent [...] Read more.
Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play a crucial regulatory role in plant biology, surpassing the essential functions of RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent an emerging class of regulators, operating directly in the form of small interfering RNAs (siRNAs), microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs). These ncRNAs exert control at various levels, including transcription, post-transcription, translation, and epigenetic. Furthermore, they interact with each other, contributing to a variety of biological processes and mechanisms associated with stress resilience. This review primarily concentrates on the recent advancements in plant ncRNAs, delineating their functions in growth and development across various organs such as root, leaf, seed/endosperm, and seed nutrient development. Additionally, this review broadens its scope by examining the role of ncRNAs in response to environmental stresses such as drought, salt, flood, heat, and cold in plants. This compilation offers updated information and insights to guide the characterization of the potential functions of ncRNAs in plant growth, development, and stress resilience in future research. Full article
(This article belongs to the Special Issue Non-Coding RNA and Their Regulatory Roles in Plant)
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