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Pathogens
Special Issues
Swine Viral Diseases: 2nd Edition
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Swine Viral Diseases: 2nd Edition

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Viral Pathogens".

Deadline for manuscript submissions: closed (31 July 2024) | Viewed by 1957

Special Issue Editor

Prof. Dr. Grzegorz Wozniakowski

E-Mail Website
Guest Editor
Department of Diagnosis and Clinical Sciences, Faculty of Biological and Veteterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska 1, 87-100 Toruń, Poland
Interests: veterinary virology; epidemiology; poultry and swine viruses
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues, 

Taking into account the importance of pig and poultry production in numerous countries in Europe, this Special Issue of Pathogens discusses the most important topics related to poultry and swine viral diseases, including aspects of epidemiology, diagnostics, prevention, and control. Therefore, I would like cordially to invite you to submit relevant papers to this Special Issue. Your valuable input will enrich the current state of knowledge and practical approaches to epidemiology and diagnosis and provide a better understanding of pig and poultry viral diseases.

Prof. Dr. Grzegorz Wozniakowski
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • epidemiology diagnostics
  • swine viral diseases
  • pig and poultry viral diseases
  • poultry production
  • prevention

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Published Papers (1 paper)

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Research

15 pages, 2885 KiB  
Article
Development of a Quadruplex RT-qPCR for the Detection of Porcine Rotaviruses and the Phylogenetic Analysis of Porcine RVH in China
by Kaichuang Shi, Hongjin Zhou, Shuping Feng, Junxian He, Biao Li, Feng Long, Yuwen Shi, Yanwen Yin and Zongqiang Li
Pathogens 2023, 12(9), 1091; https://doi.org/10.3390/pathogens12091091 - 28 Aug 2023
Cited by 2 | Viewed by 1554
Abstract
Rotavirus A species (RVA), RVB, RVC, and RVH are four species of rotaviruses (RVs) that are prevalent in pig herds, and co-infections occur frequently. In this study, a quadruplex real-time quantitative RT-PCR (RT-qPCR) for the simultaneous detection of four porcine RVs was developed [...] Read more.
Rotavirus A species (RVA), RVB, RVC, and RVH are four species of rotaviruses (RVs) that are prevalent in pig herds, and co-infections occur frequently. In this study, a quadruplex real-time quantitative RT-PCR (RT-qPCR) for the simultaneous detection of four porcine RVs was developed by designing specific primers and probes based on the VP6 gene of RVA, RVB, RVC, and RVH, respectively. The method showed high specificity and could only detect RVA, RVB, RVC, and RVH, without cross-reaction with other porcine viruses; showed excellent sensitivity, with a limit of detection (LOD) of 1.5 copies/µL for each virus; showed good repeatability, with intra-assay coefficients of variation (CVs) of 0.15–1.14% and inter-assay CVs of 0.07–0.96%. A total of 1447 clinical fecal samples from Guangxi province in China were tested using the developed quadruplex RT-qPCR. The results showed that RVA (42.71%, 618/1447), RVB (26.95%, 390/1447), RVC (42.92%, 621/1447), and RVH (13.68%, 198/1447) were simultaneously circulating in the pig herds, and the co-infection rate of different species of rotaviruses was found to be up to 44.01% (579/1447). The clinical samples were also detected using one previously reported method, and the coincidence rate of the detection results using two methods was more than 99.65%. The phylogenetic tree based on the VP6 gene sequences of RVH revealed that the porcine RVH strains from Guangxi province belonged to the genotype I5, which was closely related to Japanese and Vietnamese strains. In summary, an efficient, sensitive, and accurate method for the detection and differentiation of RVA, RVB, RVC, and RVH was developed and applied to investigate the prevalence of porcine RVs in Guangxi province, China. This study is the first to report the prevalence of porcine RVH in China. Full article
(This article belongs to the Special Issue Swine Viral Diseases: 2nd Edition)
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