Advancements in Biosurfactants: Production, Characterization and Multifaceted Applications

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: 10 July 2025 | Viewed by 1103

Special Issue Editors


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Guest Editor
Department of Organic Processes, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, RJ, Brazil
Interests: biotechnology; biosurfactants; biodegradation; fermentation; waste valorization

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Guest Editor
Department of Biochemical Engineering, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, RJ, Brazil
Interests: bioproducts; yeast; biosurfactants; byproducts conversion; enzymes
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Special Issue Information

Dear Colleagues,

Biosurfactants, amphiphilic compounds produced by microorganisms, are molecules of significant interest due to their diverse applications and environmental importance. These compounds possess unique properties that enable them to reduce surface and interfacial tensions, making them valuable in various industrial, environmental, and biomedical fields.

This Special Issue, titled "Advancements in Biosurfactants: Production, Characterization and Multifaceted Applications", aims to collate cutting-edge research in the field of biosurfactants. This collection of articles will address crucial aspects such as biosurfactant production methodologies, advanced characterization techniques, and the broad spectrum of applications across various industries. Emphasizing the environmental and industrial significance of biosurfactants, this Special Issue seeks to foster a deeper understanding of their roles in enhancing bioremediation, improving microbial enhanced oil recovery, and promoting sustainable practices. We invite contributions that showcase novel insights, methodologies, and real-world applications contributing to the continued evolution of biosurfactant science.

Dr. Tatiana Felix Ferreira
Dr. Priscilla Amaral
Guest Editors

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Keywords

  • biosurfactants
  • bioremediation
  • characterization techniques
  • environmental applications
  • microbial enhanced oil recovery (MEOR)
  • bioprospecting
  • surfactant-producing microorganisms
  • sustainable chemistry
  • interfacial properties
  • bioemulsifiers

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Published Papers (2 papers)

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Research

12 pages, 2098 KiB  
Article
Production, Characterization and Application of Biosurfactant for Cleaning Cotton Fabric and Removing Oil from Contaminated Sand
by Renata R. Silva, Maria C. F. Caldas, Carlos V. A. Lima, Hugo M. Meira, Leonie A. Sarubbo and Juliana M. Luna
Processes 2024, 12(11), 2584; https://doi.org/10.3390/pr12112584 - 18 Nov 2024
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Abstract
Biosurfactants are a group of environmentally friendly amphiphilic molecules that are applicable in numerous industries as essential biotechnology products, such as food production, cleaning products, pharmacology, cosmetics, pesticides, textiles and oil and gas fields. In this sense, and knowing the potential of these [...] Read more.
Biosurfactants are a group of environmentally friendly amphiphilic molecules that are applicable in numerous industries as essential biotechnology products, such as food production, cleaning products, pharmacology, cosmetics, pesticides, textiles and oil and gas fields. In this sense, and knowing the potential of these biomolecules, the aim of this work was to produce a biosurfactant, characterize it regarding its chemical and surfactant properties and investigate its potential in the removal of contaminants and in the cleaning of cotton fabrics. The biosurfactant was initially obtained from the cultivation of the microorganism Candida glabrata UCP 1002 in medium containing distilled water with 2.5% residual frying oil, 2.5% molasses and 2.5% corn steep liquor agitated at 200 rpm for 144 h. The biosurfactant reduced the surface tension of water from 72 to 29 mN/m. The toxicity potential of the biosurfactant was evaluated using Tenebrio molitor larvae and demonstrated non-toxicity. The biosurfactant was applied as a degreaser of engine oil on cotton fabric, and showed 83% (2× CMC), 74% (1× CMC) and 78% (1/2× CMC) oil removal. Therefore, the biosurfactant produced in this work has promising surfactant and emulsifying properties with potential for application in various industrial segments. Full article
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15 pages, 2396 KiB  
Article
Isolation and Characterization of Biosurfactant-Producing Bacteria for Enhancing Oil Recovery
by Meiyu Jiang, Hongyi Wang, Jiahui Liu, Xuan Hou, Yuanyuan Zhang, Xiaolin Liu, Shiping Wei and Qingfeng Cui
Processes 2024, 12(11), 2575; https://doi.org/10.3390/pr12112575 - 17 Nov 2024
Viewed by 397
Abstract
Biosurfactants produced by bacteria possess remarkable emulsification properties for crude oil, significantly enhancing oil mobility and recovery rates. This study aimed to isolate and screen biosurfactant-producing bacteria for oil enhancing recovery. A total of 93 bacterial strains were isolated from marine sediments, with [...] Read more.
Biosurfactants produced by bacteria possess remarkable emulsification properties for crude oil, significantly enhancing oil mobility and recovery rates. This study aimed to isolate and screen biosurfactant-producing bacteria for oil enhancing recovery. A total of 93 bacterial strains were isolated from marine sediments, with three high-yield biosurfactant-producing strains identified: Pseudomonas aeruginosa N33, Bacillus paralicheniformis Nian2, and Stenotrophomonas nematodicola T10. The fermentation conditions, such as pH, carbon source, nitrogen source, and C/N ratio, were optimized to maximize the yield and activity of biosurfactants. Further evaluations were performed to assess the stability of the bio-surfactant activity and its emulsification properties. The results indicated that all three strains produced biosurfactants that retained their oil displacement activity in the presence of Na+ and Mg2+, but showed a significant reduction in their activities in the presence of Ca2+. The biosurfactants maintained their original activity after treatment at 120 °C for 3 h. Additionally, the biosurfactants produced by all three strains demonstrated excellent oil emulsification capabilities. Static oil-washing and dynamic displacement experiments revealed static oil recovery rates of 28.1%, 23.4%, and 7.1%, respectively, for N33, Nian2, and T10, and dynamic oil displacement recovery rates of 95.0%, 74.1%, and 69.0%, respectively. This research provides valuable microbial resources for enhancing oil recovery via microorganisms and lays a foundation for practical application. Full article
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