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Recent Advances in Photoelectrochemical Sensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: 25 December 2024 | Viewed by 1186

Special Issue Editors


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Guest Editor
Department of Biophysics, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061, China
Interests: photoelectrochemistry; single-cell imaging; multiplexed sensing; biosensors; semiconductors; spatial resolution; light-addressable electrochemical sensors
Institute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an 710061, China
Interests: chemical sensors; biomimetic olfactory biosensors; odor discrimination; bioelectronic nose; olfac-tory organoid
Special Issues, Collections and Topics in MDPI journals
Department of Biophysics, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an, China
Interests: biosensors; bacterial cellulose; polymer composite; electrochemical sensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Photoelectrochemical (PEC) sensing is an analytical technique that combines the principles of photoelectrochemistry and traditional sensing methods to detect and quantify bio(chemical) substances. It leverages the interaction between light and materials to generate an electrical signal, which is correlated with the concentration of the analyte. PEC sensors typically involve a photoactive material, such as a semiconductor, that absorbs light and generates charge carriers (electrons and holes). These carriers participate in redox reactions at the electrode surface, thus producing a measurable current. The method offers high sensitivity due to the unique setup consisting of two separate energy forms, that is, using light as the excitation source and electricity as the detection signal. Applications of PEC sensing are diverse, ranging from environmental monitoring and food safety to clinical diagnostics and biochemical analysis.

This Special Issue will cover the system, instruments, methods, and photoactive and bio-sensitive materials used to construct PEC sensors with various bio(chemical) sensing applications, such as investigations of living cells, organoids, or tissues, as well as detections of ions, bio(chemical) molecules, or metabolites.

Dr. Jian Wang
Dr. Liping Du
Dr. Wei Chen
Guest Editors

Manuscript Submission Information

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Keywords

  • light-addressable electrochemical sensors
  • light addressability
  • photocurrent
  • photoactive materials
  • bio-sensitive materials
  • photoelectrochemistry
  • multiplexed detection
  • bio(chemical) imaging
  • bio(chemical) sensing

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Published Papers (1 paper)

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Research

13 pages, 3459 KiB  
Article
A Photoelectrochemical Biosensor Mediated by CRISPR/Cas13a for Direct and Specific Detection of MiRNA-21
by Yang Zhang, Pei Miao, Jingyuan Wang, Yan Sun, Jing Zhang, Bin Wang and Mei Yan
Sensors 2024, 24(18), 6138; https://doi.org/10.3390/s24186138 - 23 Sep 2024
Viewed by 894
Abstract
Direct detection of miRNA is currently limited by the complex amplification and reverse transcription processes of existing methods, leading to low sensitivity and high operational demands. Herein, we developed a CRISPR/Cas13a-mediated photoelectrochemical (PEC) biosensing platform for direct and sensitive detection of miRNA-21. The [...] Read more.
Direct detection of miRNA is currently limited by the complex amplification and reverse transcription processes of existing methods, leading to low sensitivity and high operational demands. Herein, we developed a CRISPR/Cas13a-mediated photoelectrochemical (PEC) biosensing platform for direct and sensitive detection of miRNA-21. The direct and specific recognition of target miRNA-21 by crRNA-21 eliminates the need for pre-amplification and reverse transcription of miRNA-21, thereby preventing signal distortion and enhancing the sensitivity and precision of target detection. When crRNA-21 binds to miRNA-21, it activates the trans-cleavage activity of CRISPR/Cas13a, leading to the non-specific cleavage of biotin-modified DNA with uracil bases (biotin-rU-DNA). This cleavage prevents the biotin-rU-DNA from being immobilized on the electrode surface. As a result, streptavidin cannot attach to the electrode via specific biotin binding, reducing spatial resistance and causing a positively correlated increase in the photocurrent response. This Cas-PEC biosensor has good analytical capabilities, linear responses between 10 fM and 10 nM, a minimum detection limit of 9 fM, and an excellent recovery rate in the analysis of real human serum samples. This work presented an innovative solution for detecting other biomarkers in bioanalysis and clinical diagnostics. Full article
(This article belongs to the Special Issue Recent Advances in Photoelectrochemical Sensors)
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