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Integrated Microfluidic Microarray Biochip and Biosensor 2021-2022

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (20 August 2022) | Viewed by 3514

Special Issue Editor


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Guest Editor
National Heart Centre Singapore, 5 Hospital Drive, Singapore 169609, Singapore
Interests: microfluidics; 3D cell cultures; human-on-a-chip; lab automation; biomedical engineering
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Special Issue Information

Dear Colleagues,

Microarray is a concept implementing multiple probes for a high-throughput parallel analysis. Over the two decades since the invention of the concept, several types of probes, ranging from small molecules, oligonucleotides, antibodies, proteins, and even whole organisms, such as viruses (e.g., bacteriophage), bacteria cells, and mammalian cells, have been utilized for high-throughput sensing in various types of research, such as biosensing and molecular biology studies. When integrated with microfluidic devices, the microarray technique becomes more powerful by reducing the reagent consumption and reaction time, with precise control of the microenvironment, and having a higher compatibility with the automated operation. This Issue intends to cover the technology involved in the integration of microarray and microfluidics, and the benefits/applications made possible by the integration.

Prof. Dr. Danny van Noort
Guest Editor

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Published Papers (1 paper)

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Research

23 pages, 20483 KiB  
Article
Microfluidic-Assisted Human Cancer Cells Culturing Platform for Space Biology Applications
by Agnieszka Krakos (Podwin), Joanna Jarosz, Patrycja Śniadek, Mateusz Psurski, Adrianna Graja, Marcin Białas, Ewa Oliszewska, Joanna Wietrzyk, Rafał Walczak and Jan Dziuban
Sensors 2022, 22(16), 6183; https://doi.org/10.3390/s22166183 - 18 Aug 2022
Cited by 4 | Viewed by 2589
Abstract
In the paper, the lab-on-chip platform applicable for the long-term cultivation of human cancer cells, as a solution meeting the demands of the CubeSat biological missions, is presented. For the first time, the selected cancer cell lines—UM-UC-3 and RT 112 were cultured on-chip [...] Read more.
In the paper, the lab-on-chip platform applicable for the long-term cultivation of human cancer cells, as a solution meeting the demands of the CubeSat biological missions, is presented. For the first time, the selected cancer cell lines—UM-UC-3 and RT 112 were cultured on-chip for up to 50 days. The investigation was carried out in stationary conditions (without medium microflow) in ambient temperature and utilizing the microflow perfusion system in the incubation chamber assuring typical cultivation atmosphere (37 °C). All the experiments were performed to imitate the conditions that are provided before the biological mission starts (waiting for the rocket launch) and when the actual experiment is initialized on a CubeSat board in space microgravity. The results of the tests showed appropriate performance of the lab-on-chip platform, especially in the context of material and technological biocompatibility. Cultured cells were characterized by adequate morphology—high attachment rate and visible signs of proliferation in each of the experimental stage. These results are a good basis for further tests of the lab-on-chip platform in both terrestrial and space conditions. At the end of the manuscript, the authors provide some considerations regarding a potential 3-Unit CubeSat biological mission launched with Virgin Orbit company. The lab-on-chip platform was modelled to fit a 2-Unit autonomous laboratory payload. Full article
(This article belongs to the Special Issue Integrated Microfluidic Microarray Biochip and Biosensor 2021-2022)
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