Mass Spectrometry Development and Its Application in Bioanalysis

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Bioanalysis/Clinical Analysis".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 4302

Special Issue Editors


E-Mail Website
Guest Editor
Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, NJ 07030, USA
Interests: mass spectrometry; mass spectrometric ion fragmentations; bioanalytical chemistry; microchemical techniques; chromatography; ion mobility separations

E-Mail Website
Guest Editor
Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, NJ 07030, USA
Interests: mass spectrometry

Special Issue Information

Dear Colleagues,

Mass spectrometry-based techniques are among the leading methods applied to characterize and quantify various biologically relevant small and large biomolecules such as sugars, lipids, proteins, and oligonucleotides. It is a rapidly developing, highly dynamic field. Novel developments are continuously added. Particularly, when combined with chromatographic and ion-mobility separation, mass spectrometry provides a method unrivaled by any other analytical method in terms of versatility, speed of sample analysis, and limits of detectability. This Special Issue aims to collect original research papers and review articles on novel developments in mass spectrometry-based techniques for biological analysis.

Prof. Dr. Athula Attygalle
Dr. Julius Pavlov
Guest Editor

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Keywords

  • mass spectrometry
  • biomolecules
  • chromatographic separation
  • ion-mobility separation
  • biological analysis

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Published Papers (1 paper)

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Research

17 pages, 1944 KiB  
Article
Salting-Out Assisted Liquid-Liquid Extraction for UPLC-MS/MS Determination of Thyroxine and Steroid Hormones in Human Serum and Fish Plasma
by Alemnesh Yirda Urge, Daniela Maria Pampanin, Maria Elena Martino, David Lausten Knudsen and Cato Brede
Separations 2023, 10(4), 240; https://doi.org/10.3390/separations10040240 - 5 Apr 2023
Cited by 2 | Viewed by 3972
Abstract
Measuring the level of steroid and thyroxine hormones is key to understanding organism health conditions. Liquid chromatography coupled with tandem mass spectrometry has become the method of choice for such hormone analyses in clinical laboratories. Detection of hormones at low levels typically requires [...] Read more.
Measuring the level of steroid and thyroxine hormones is key to understanding organism health conditions. Liquid chromatography coupled with tandem mass spectrometry has become the method of choice for such hormone analyses in clinical laboratories. Detection of hormones at low levels typically requires a time-consuming sample preparation, such as liquid-liquid extraction followed by solvent evaporation and re-solubilization of the sample extract. Instead, we applied salting-out assisted liquid-liquid extraction (SALLE) for the extraction of thyroxine, testosterone, cortisone, and cortisol from human serum and fish plasma samples. SALLE allowed direct injection of sample extracts. Sodium chloride and ammonium sulfate were evaluated as salting-out reagents together with four different organic solvents. High extraction recovery and reduced matrix interference were achieved by using ammonium sulfate together with 10% methanol in acetonitrile. Limits of quantification were in the range of 0.1–0.2 ng/mL and signal responses were linear (R2 > 0.997) up to at least 100 ng/mL for all hormones. The method was applied for hormone measurements in fish plasma. In conclusion, SALLE combines the simplicity of crude protein precipitation with the high analyte enrichment of a liquid-liquid extraction. Here we have presented it as a novel sample preparation method for clinical laboratories where mass spectrometry is utilized in the field of endocrinology. Full article
(This article belongs to the Special Issue Mass Spectrometry Development and Its Application in Bioanalysis)
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