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Analysis of Natural Products and Synthetic Drugs by HPLC or...
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Analysis of Natural Products and Synthetic Drugs by HPLC or HPTLC

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (30 May 2023) | Viewed by 4777

Special Issue Editor

Prof. Dr. Prawez Alam

E-Mail Website
Guest Editor
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdul aziz University, Al-Kharj 11942, Saudi Arabia
Interests: HPTLC; HPLC; GC-MS; quality control of herbal drugs; pharmaceutical analysis; phytochemical analysis

Special Issue Information

Dear Colleagues,

There are many physiologically active molecules in natural products as well as synthetic drugs. Traditional medicinal plants contain an abundance of well-known secondary metabolites such as alkaloids, flavonoids, saponin, steroids, terpines, triterpenoids, total phenol, and total flavonoids. One of the most important methods in the study of natural products and synthetic drugs is chromatography, particularly high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC). There are many different types of bioactive herbal compounds and synthetic drugs available on the market with a wide range of biological activity. These plant-based chemicals exhibit a range of biological properties, from antioxidant to anticancer action. For the qualitative and quantitative examination of biomarker chemicals found in diverse plants and pharmaceutical products, HPLC and HPTLC play a significant role. This Special Issue seeks to combine the most recent developments, breakthroughs, and analytical innovations in HPLC and HPTLC techniques for the identification and analysis of natural bioactive substances and synthetic drugs. Additionally, we want to make it possible for researchers to correlate plant phytochemical profiles with well-established therapeutic benefits, which could later support the health claims made for these products.

Prof. Dr. Prawez Alam
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography
  • chromatographic screening
  • chemical profiling
  • stationary phase
  • HPTLC
  • phytochemical analysis
  • fingerprint profile
  • HPLC
  • method validation

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Published Papers (3 papers)

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Research

13 pages, 2020 KiB  
Article
Stability-Indicating Quantification of Ciprofloxacin in the Presence of Its Main Photo-Degradation Product by CZE and UPLC: A Comparative Study
by Alhumaidi B. Alabbas and Sherif A. Abdel-Gawad
Separations 2023, 10(7), 391; https://doi.org/10.3390/separations10070391 - 3 Jul 2023
Cited by 2 | Viewed by 2288
Abstract
Active pharmaceutical ingredients (APIs) can be assured to be safe and effective with the help of stability-indicating procedures. An accurate comparison comprising the utilization of capillary zone electrophoresis (CZE) and ultra-performance liquid chromatography (UPLC) for the sensitive and accurate measurement of ciprofloxacin (CPF) [...] Read more.
Active pharmaceutical ingredients (APIs) can be assured to be safe and effective with the help of stability-indicating procedures. An accurate comparison comprising the utilization of capillary zone electrophoresis (CZE) and ultra-performance liquid chromatography (UPLC) for the sensitive and accurate measurement of ciprofloxacin (CPF) in the presence of its major photo-degradation product was conducted. The CZE and UPLC working conditions were optimized to obtain the best pattern of separation for CPF and its photo-degradant. The linearity range of the cited techniques was confirmed to be 0.5 to 50 µg/mL. A thorough validation scheme according to the ICH-Q2B criteria was performed, including linearity, accuracy, precision, robustness, detection, and quantification limits. Selective quantification of CPF in the presence of up to 90% of its main photo-degradant was carried out using the proposed methods. For the analysis of CPF in tablet and intravenous (I.V.) solution forms, the CZE and UPLC procedures were applied. The suggested methods can be applied to keep an eye on the safety and efficacy of CPF in either bulk or dosage forms. Full article
(This article belongs to the Special Issue Analysis of Natural Products and Synthetic Drugs by HPLC or HPTLC)
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15 pages, 3221 KiB  
Article
A Simple Stability-Indicating UPLC Method for the Concurrent Assessment of Paracetamol and Caffeine in Pharmaceutical Formulations
by Wasim Ahmad, Yousif Amin Hassan, Ayaz Ahmad, Manal Suroor, Mohammad Sarafroz, Prawez Alam, Shadma Wahab and Shahana Salam
Separations 2023, 10(1), 50; https://doi.org/10.3390/separations10010050 - 12 Jan 2023
Cited by 2 | Viewed by 2955
Abstract
A fixed-dose combination of paracetamol (PCM) and caffeine (CAF) tablets/capsules is the most frequently used over-the-counter medicine for fever and headache. In this paper, a simple, reliable, sensitive, rapid, and stability-indicating ultra-performance liquid chromatography (UPLC) analytical method was proposed for simultaneously assessing PCM [...] Read more.
A fixed-dose combination of paracetamol (PCM) and caffeine (CAF) tablets/capsules is the most frequently used over-the-counter medicine for fever and headache. In this paper, a simple, reliable, sensitive, rapid, and stability-indicating ultra-performance liquid chromatography (UPLC) analytical method was proposed for simultaneously assessing PCM and CAF in pharmaceutical formulations. The UPLC method was developed on an Acquity UPLC® CSHTM C18 column, and the column oven temperature was maintained at 35 ± 5 °C with isocratic elution by using a solution of methanol and water (30:70, v/v). The maximum absorbance of PCM and CAF was observed at 272.5 nm. The flow rate was 0.2 mL/min, and the injection volume was 1 µL, with the total run time of 2 min for the separation of PCM and CAF. The proposed UPLC method was validated according to the ICH guidelines, and it demonstrated excellent linearity, with correlation coefficients of 0.9995 and 0.9999 over the concentration ranges of 40–400 and 7–70 ng/mL for PCM and CAF, respectively. The mean retention times of 0.82 ± 0.0 and 1.16 ± 0.02 were observed for PCM and CAF, respectively. The limits of detection and quantification were 16.62 and 3.86 for PCM, respectively, and 50.37 and 11.70 for CAF, respectively. PCM and CAF were subjected to acidic, alkali, oxidative, phytochemical, dry-heat, and wet-heat degradation. The method was found to well separate the analytes’ peaks from degradation peaks, with no alterations in retention times. The proposed method is linear, precise, accurate, specific, and robust, and it can indicate stability and be used for the quantitative assessment of pharmaceutical formulations comprising PCM and CAF within a short period of time. Full article
(This article belongs to the Special Issue Analysis of Natural Products and Synthetic Drugs by HPLC or HPTLC)
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12 pages, 2183 KiB  
Article
Development and Validation of a Stability-Indicating Greener HPTLC Method for the Estimation of Flufenamic Acid
by Prawez Alam, Faiyaz Shakeel, Mohammed H. Alqarni, Ahmed I. Foudah, Tariq M. Aljarba, Mohammed M. Ghoneim, Syed Mohammed Basheeruddin Asdaq and Sultan Alshehri
Separations 2023, 10(1), 39; https://doi.org/10.3390/separations10010039 - 8 Jan 2023
Cited by 6 | Viewed by 1796
Abstract
The literature on ecofriendly/greener high-performance thin-layer chromatographic (HPTLC) methods for quantifying flufenamic acid (FFA) is scant. In order to develop and validate a stability-indicating greener HPTLC densitometry assay for FFA determination in marketed products, this research was conducted. The ecofriendly eluent system was [...] Read more.
The literature on ecofriendly/greener high-performance thin-layer chromatographic (HPTLC) methods for quantifying flufenamic acid (FFA) is scant. In order to develop and validate a stability-indicating greener HPTLC densitometry assay for FFA determination in marketed products, this research was conducted. The ecofriendly eluent system was composed of ethanol–water (70:30 v/v). FFA was measured at 290 nm of wavelength. The greenness scale of suggested analytical assay was derived using “Analytical GREENness (AGREE)” methodology. The suggested stability-indicating HPTLC assay was linear for FFA determination in 25–1400 ng/band range with a determination coefficient of 0.9974. The suggested analytical assay for FFA analysis was simple, rapid, accurate, precise, robust, selective, stability-indicating, and greener. The AGREE scale for the developed stability-indicating HPTLC assay was derived to be 0.77 utilizing AGREE methodology, indicating an outstanding greenness characteristic of the suggested densitometry technique. The ecofriendly HPTLC technique was able to detect FFA degradation product under forced degradation studies, indicating its stability-indication characteristics and selectivity. The amount of FFA in marketed tablets brand A and B was determined to be 101.28 and 99.17%, respectively, indicating the suitability of the suggested analytical technique in the assay of FFA in marketed products. These results indicated that FFA in marketed products may be routinely measured using the stability-indicating greener HPTLC technique. Full article
(This article belongs to the Special Issue Analysis of Natural Products and Synthetic Drugs by HPLC or HPTLC)
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