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Separations
Special Issues
Extraction, Purification and Application of Bioactive Compounds
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Extraction, Purification and Application of Bioactive Compounds

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: closed (31 October 2024) | Viewed by 3573

Special Issue Editors

Dr. Naoufal El Hachlafi

E-Mail Website
Guest Editor
Laboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Technologies, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Imouzzer Road, Fez, Morocco
Interests: bioactive compounds; adsorptive removal of heavy metals; microbiology; pharmaceuticals; biological properties; experimental design; chemical characterization; drug discovery; food products; development of new antimicrobial agents
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Hanae Naceiri Mrabti

E-Mail Website
Guest Editor
1. Laboratory of Pharmacology and Toxicology, Bi o Pharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, University Mohammed V in Rabat, Raba, Morocco
2. Euromed Research Center, Euromed Faculty of Pharmacy and School of Engineering and Biotechnology, Euromed University of Fes (UEMF), Meknes Road, Fez 30000, Morocco
Interests: diabetes; bioactive compounds; microbiology; food products; honey; GC-MS analysis; volatile compounds; drug discovery
Dr. Amine Elbouzidi

E-Mail Website
Guest Editor
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, Morocco
Interests: biotechnology; secondary metabolites; isolation methods; chromatography; volatile compounds; pharmacological activities

Special Issue Information

Dear Colleagues,

The extraction and purification of bioactive compounds from natural sources is a key focus area in research and industry. This process involves isolating compounds with medicinal or functional properties from plants, microorganisms, and other biological materials.

Extraction methods vary depending on factors such as the target compound, source material, and desired purity. Common techniques include solvent extraction, supercritical fluid extraction, and solid-phase extraction. After extraction, purification steps are often necessary to remove impurities and concentrate the bioactive compounds. The procedures chosen for this may include chromatographic methods, such as column chromatography, high-performance liquid chromatography (HPLC), and preparative thin-layer chromatography (TLC), or methods like crystallization, filtration, and distillation, depending on the properties of the compounds and the desired level of purity. Overall, the extraction and purification of bioactive compounds play a crucial role in harnessing the therapeutic and functional potential of natural sources for various applications in pharmaceuticals, nutraceuticals and cosmetics.

The aim of this Special Issue is to provide information on recent developments in the chemical investigation of bioactive compounds, emphasizing the extraction, separation and analysis of these compounds via chromatographic and spectral techniques.

Dr. Naoufal El Hachlafi
Prof. Dr. Hanae Naceiri Mrabti
Dr. Amine Elbouzidi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • extraction
  • chemical characterization
  • pharmacological properties
  • bioactive compounds
  • GC-MS analysis
  • biotechnological application

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Published Papers (3 papers)

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Research

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15 pages, 1481 KiB  
Article
Chemical Composition and Biological Properties of Achillea cucullata Extracts from Leaves and Flowers
by Manal Abdulaziz Binobead and Ibrahim M. Aziz
Separations 2024, 11(8), 236; https://doi.org/10.3390/separations11080236 - 1 Aug 2024
Viewed by 858
Abstract
Achillea cucullata is a perennial herbaceous plant that has a long history of medical use in many cultures. The present research focuses on the biological activity and therapeutic potential of A. cucullata, namely its antibacterial and anticancer properties. While previous studies have [...] Read more.
Achillea cucullata is a perennial herbaceous plant that has a long history of medical use in many cultures. The present research focuses on the biological activity and therapeutic potential of A. cucullata, namely its antibacterial and anticancer properties. While previous studies have shed light on the cytotoxic and antibacterial capabilities of Achillea cucullata aerial parts, there is still a considerable gap in knowledge concerning the anticancer potential of leaf and flower extracts. A. cucullata’s leaves and flowers were extracted using methanol. The total phenolic and flavonoid contents were evaluated. The antioxidant, cytotoxic, and antibacterial properties were evaluated against both Gram-positive and Gram-negative bacteria. The Gas Chromatography–Mass Spectrometry (GC–MS) analysis of A. cucullata leaf and flower extracts showed numerous amounts of bioactive components, including carvacrol, a TBDMS derivative; 2-Myristynoyl-glycinamide, acetylaminobenzothiazol-2-yl)-2-(adamantan-1-yl); Isolongifolol; (3E,10Z)-Oxacyclotrideca-3,10-diene-2,7-dione; and 3-Heptanone, 5-hydroxy-1,7-diphenyl. The extract has a high level of phenols and flavonoids. Cytotoxicity studies found that A. cucullata leaves and flowers had dose-dependent toxicity against MCF-7 and HepG2 cancer cell lines, with flowers being more effective. Apoptotic genes (caspase-3, 8, 9, and Bax) were upregulated in treated MCF-7 and HepG2 cells, whereas anti-apoptotic genes (Bcl-xL and Bcl-2) were reduced. Antibacterial screening revealed significant activity against both Gram-positive and Gram-negative pathogens. Overall, the research highlights the varied therapeutic potentials of A. cucullata, adding to the knowledge of plant-derived extracts in lowering disease risks. Future research should concentrate on in vivo studies to assess the effectiveness and safety of these substances. Full article
(This article belongs to the Special Issue Extraction, Purification and Application of Bioactive Compounds)
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25 pages, 5094 KiB  
Article
Extraction of Bioactive Compound-Rich Essential Oil from Cistus ladanifer L. by Microwave-Assisted Hydrodistillation: GC-MS Characterization, In Vitro Pharmacological Activities, and Molecular Docking
by Naoufal El Hachlafi, Fahd Kandsi, Amine Elbouzidi, Fatima Zahra Lafdil, Ghizlane Nouioura, Emad M. Abdallah, Rhizlan Abdnim, Mohamed Bnouham, Samiah Hamad Al-Mijalli, Hanae Naceiri Mrabti and Kawtar Fikri-Benbrahim
Separations 2024, 11(7), 199; https://doi.org/10.3390/separations11070199 - 27 Jun 2024
Cited by 2 | Viewed by 1494
Abstract
Cistus ladanifer L. is an aromatic and resinous perennial shrub commonly used in Moroccan folk medicine against a range of illnesses including skin problems, diabetes, diarrhea, and inflammation. The current investigation aims to determine the bioactive compounds of C. ladanifer essential oil (CL-Eo) [...] Read more.
Cistus ladanifer L. is an aromatic and resinous perennial shrub commonly used in Moroccan folk medicine against a range of illnesses including skin problems, diabetes, diarrhea, and inflammation. The current investigation aims to determine the bioactive compounds of C. ladanifer essential oil (CL-Eo) extracted by microwave-assisted hydrodistillation and their biological properties using in vitro and molecular docking approaches. The GC-MS analysis identified linderol (17.76%), gamma-terpinene (17.55%), and borneol (13.78%) as main bioactive compounds. CL-Eo significantly inhibited α-amylase (IC50 = 0.41 ± 0.009 mg/mL), α-glucosidase (IC50 = 0.49 ± 0.002 mg/mL) and lipase (IC50 = 0.45 ± 0.004 mg/mL) enzymes. Moreover, CL-Eo showed significant hemoglobin glycation as well as antioxidant capacity as indicated by DPPH, ABTS, Frap and beta-carotene tests. The antimicrobial evaluation used disc-diffusion and microdilution tests in vitro. The results showed that CL-Eo had significant antibacterial activity, particularly against P. mirabilis (17.16 ± 1.04 mm), and moderate effects against L. innocua (13.48 ± 1.65 mm) and E. coli (12.47 ± 0.61 mm). In addition, it demonstrated potent antifungal activity against C. albicans (18.01 ± 0.91 mm) and C. tropicalis (16.45 ± 0.32 mm). The MIC and MBC tests provided confirmation that CL-Eo exhibited potent growth inhibition. The MIC ranged from 0.25 to 8.0% v/v and the MBC or MFC ranged from 0.25 to 16.0% v/v Eo. The tolerance level ratio showed bactericidal and fungicidal effects against tested microbial strains in varying degrees. According to these data, CL-Eo might be suggested as a promising candidate for drug development, specifically for combating candidiasis and diabetes. Full article
(This article belongs to the Special Issue Extraction, Purification and Application of Bioactive Compounds)
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Review

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27 pages, 3104 KiB  
Review
Developments in the Dry Fractionation of Plant Components: A Review
by Ganapathy Subramanian Meenakshi Sundaram, Divyapratim Das, Tolu Emiola-Sadiq, Abdullah Sajeeb Khan, Lifeng Zhang and Venkatesh Meda
Separations 2024, 11(12), 332; https://doi.org/10.3390/separations11120332 - 21 Nov 2024
Viewed by 277
Abstract
Over the years, pulses and cereals have been identified as promising sources of plant proteins. The intensive production of these crops and concerns about food security and malnutrition worldwide have intensified research into their separation. While wet extraction remains the standard protein isolation [...] Read more.
Over the years, pulses and cereals have been identified as promising sources of plant proteins. The intensive production of these crops and concerns about food security and malnutrition worldwide have intensified research into their separation. While wet extraction remains the standard protein isolation method, the search for more sustainable extraction methods is still ongoing. Two dry fractionation techniques, air classification and tribo-electrostatic separation, have been discussed in this review. This review highlights the design aspects of air classifiers including the cut-off point and flow rate, and for electrostatic separators, factors such as charger materials, the nature of the flow in charger tubes, and the strength of the electric field potential have been discussed in detail. Our analysis revealed that cascading the two techniques should help enhance the concentration and purity of the separated fractions. While limitations such as low purity and low yield exist, current research studies are focused on overcoming such drawbacks. Dry fractionation exhibits potential as a sustainable processing method while also preserving the native functionality of the proteins, making it easier to incorporate the fractions in commercial scale processes. Full article
(This article belongs to the Special Issue Extraction, Purification and Application of Bioactive Compounds)
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