Electrophoretic Methodologies for Protein Analysis

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 10994

Special Issue Editor


E-Mail Website
Guest Editor
Institut des Biomolécules Max Mousseron (IBMM), Université de Montpellier– CNRS, UMR 5247, 34095 Montpellier, France
Interests: electrophoretic methodologies; miniaturized analytical techniques; pharmaceutical analysis; biomolecule analysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Electrophoretic methodologies are essential in the field of protein analysis whether for characterization, purification, quantification or protein–protein interaction purposes. In addition to mild operating conditions, the versatility of electrophoretic protocols provides unique separation abilities suitable for the analysis of both hydrophilic and hydrophobic proteins in various matrices. The potential of electrophoretic methods for miniaturization as well as their recent hyphenation to mass spectrometry has dramatically expanded their application areas both for top–down to bottom–up approaches.

The target of this Special Issue is to present the state of art in protein analysis by electrophoretic methodologies in conventional, capillary, and microchip format. Recent, original, and efficient methodologies are reported for all protein analysis domains.

Prof. Dr. Catherine Perrin
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Capillary electrophoresis
  • Electrophoretic methodologies
  • Electrophoretic µ-chip
  • Electrophoretic techniques and hyphenation
  • Free solution electrophoresis
  • Isoelectric focusing (IEF)
  • Polyacrylamide gel electrophoresis (PAGE)—sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • Protein analysis
  • Protein characterization
  • Protein–protein interactions
  • Protein purification

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Review

30 pages, 1376 KiB  
Review
Analysis of Monoclonal Antibodies by Capillary Electrophoresis: Sample Preparation, Separation, and Detection
by Meriem Dadouch, Yoann Ladner and Catherine Perrin
Separations 2021, 8(1), 4; https://doi.org/10.3390/separations8010004 - 4 Jan 2021
Cited by 31 | Viewed by 17176
Abstract
Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microheterogeneities that may affect their safety, quality, and efficacy. This [...] Read more.
Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microheterogeneities that may affect their safety, quality, and efficacy. This complexity is very challenging for mAbs development, formulation, and quality control. To tackle the quality issue, a combination of multiple analytical approaches is necessary. In this perspective, capillary electrophoresis has gained considerable interest over the last decade due to the fact of its complementary features to chromatographic approaches. This review provides an overview of the strategies of mAbs and derivatives analysis by capillary electrophoresis hyphenated to ultraviolet, fluorescence, and mass spectrometry detection. The main sample preparation approaches used for mAb analytical characterization (i.e., intact, middle-up/down, and bottom-up) are detailed. The different electrophoretic modes used as well as integrated analysis approaches (sample preparation and separation) are critically discussed. Full article
(This article belongs to the Special Issue Electrophoretic Methodologies for Protein Analysis)
Show Figures

Figure 1

Back to TopTop