mRNA Vaccines and Monoclonal Antibodies for Therapy

A special issue of Vaccines (ISSN 2076-393X). This special issue belongs to the section "Therapeutic Vaccines and Antibody Therapeutics".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 721

Special Issue Editors


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Guest Editor
Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Messina 98124, Italy
Interests: autoimmune; vaccination; public health, health promotion

Special Issue Information

Dear Colleagues,

mRNA vaccines and monoclonal antibodies are emerging, promising treatments. mRNA vaccines contain messenger RNA (mRNA) which carries instructions to produce a specific protein. Once inside our body's cells, the mRNA acts as a blueprint to create a viral protein. Then, this protein induces specific antibodies. These antibodies persist for between 4 and 6 months, but immune cells remember how to defend against future infections. mRNA vaccines and monoclonal antibodies show promise in personalized medicine, thereby offering hope for improved outcomes and reduced side effects compared to those of traditional treatments. This Special Issue aims to provide insights into the safe, effective use of mRNA vaccines and of monoclonal antibodies. Novel articles, reviews, and preclinical studies that use human-relevant models or studies on humans are encouraged.

Dr. Giovanni Genovese
Dr. Cristina Genovese
Guest Editors

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Keywords

  • vaccinations
  • vaccines
  • immunotherapy
  • active immunization

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Published Papers (1 paper)

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Research

13 pages, 3261 KiB  
Article
Lung-Selective Delivery of mRNA-Encoding Anti-MERS-CoV Nanobody Exhibits Neutralizing Activity Both In Vitro and In Vivo
by Yuhang Zhang, Chongyu Tian, Xinyang Yu, Guocan Yu, Xuelian Han, Yuan Wang, Haisheng Zhou, Shuai Zhang, Min Li, Tiantian Yang, Yali Sun, Wanbo Tai, Qi Yin and Guangyu Zhao
Vaccines 2024, 12(12), 1315; https://doi.org/10.3390/vaccines12121315 - 24 Nov 2024
Viewed by 407
Abstract
Background/Objectives: The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a highly pathogenic virus causing severe respiratory illness, with limited treatment options that are mostly supportive. The success of mRNA technology in COVID-19 vaccines has opened avenues for antibody development against MERS-CoV. mRNA-based [...] Read more.
Background/Objectives: The Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a highly pathogenic virus causing severe respiratory illness, with limited treatment options that are mostly supportive. The success of mRNA technology in COVID-19 vaccines has opened avenues for antibody development against MERS-CoV. mRNA-based antibodies, expressed in vivo, offer rapid adaptability to viral mutations while minimizing long-term side effects. This study aimed to develop a lung-targeted lipid nanoparticle (LNP) system for mRNA-encoding neutralizing nanobodies against MERS-CoV, proposing a novel therapeutic strategy. Methods: An mRNA-encoding nanobody NbMS10 (mRNA-NbMS10) was engineered for enhanced stability and reduced immunogenicity. This mRNA was encapsulated in lung-selective LNPs using microfluidics to form the LNP-mRNA-NbMS10 system. Efficacy was assessed through in vitro assays and in vivo mouse studies, focusing on antigen-binding, neutralization, and sustained nanobody expression in lung tissues. Results: The LNP-mRNA-NbMS10 system expressed the nanobody in vitro, showing strong antigen-binding and significant MERS-CoV pseudovirus neutralization. In vivo studies confirmed selective lung mRNA delivery, with high nanobody expression sustained for up to 24 h, confirming lung specificity and prolonged antiviral activity. Conclusions: Extensive in vitro and in vivo evaluations demonstrate the LNP-mRNA-NbMS10 system’s potential as a scalable, cost-effective, and adaptable alternative to current MERS-CoV therapies. This innovative platform offers a promising solution for preventing and treating respiratory infections, and countering emerging viral threats. Full article
(This article belongs to the Special Issue mRNA Vaccines and Monoclonal Antibodies for Therapy)
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