Purification of Plant Extracts

Topic Information

Dear Colleagues,

Separation is one of the chemical reactions that occurs between compounds and solid phases and/or mobile phases. Their combinations are infinite, and every compound should be separated into a single pure form. We are happy to invite colleagues to submit papers outlining examples of compounds and their separation methods.

In addition to this, plants are widely recognized as one of the biggest sources of active compounds used in medicine and functional foods. However, there are various plants yet to be investigated for new novel compounds.

Thus, this Topic is focused on the purification of bioactive natural products from plant resources as well as the various chromatography approaches to analyse these products, including GC and HPLC. Papers on the isolation and structural determination of novel compounds of plant origin, as well as on methods used for their analysis, are welcome for submission.

Dr. Kazuya Murata
Prof. Dr. Toshio Morikawa
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Foods foods	4.7	7.4	2012	14.3 Days	CHF 2900
Molecules molecules	4.2	7.4	1996	15.1 Days	CHF 2700
Plants plants	4.0	6.5	2012	18.2 Days	CHF 2700
Processes processes	2.8	5.1	2013	14.4 Days	CHF 2400
Separations separations	2.5	3.0	2014	12.4 Days	CHF 2600

MDPI Topics is cooperating with Preprints.org and has built a direct connection between MDPI journals and Preprints.org. Authors are encouraged to enjoy the benefits by posting a preprint at Preprints.org prior to publication:

1. Immediately share your ideas ahead of publication and establish your research priority;
2. Protect your idea from being stolen with this time-stamped preprint article;
3. Enhance the exposure and impact of your research;
4. Receive feedback from your peers in advance;
5. Have it indexed in Web of Science (Preprint Citation Index), Google Scholar, Crossref, SHARE, PrePubMed, Scilit and Europe PMC.

Published Papers (3 papers)

Order results

Content type Publication Date

Result details

Normal Extended Compact

Journals

All Foods Molecules Plants Processes Separations

Show export options expand_more Show export options expand_less

Select all

Export citation of selected articles as:

Plain Text BibTeX BibTeX (without abstracts) Endnote Endnote (without abstracts) Tab-delimited RIS

Error

Oops... you haven't selected anything for export.

Ok

clear

attachment

Supplementary material:
Supplementary File 1 (ZIP, 127 KiB)

14 pages, 3696 KiB  

Open AccessArticle

Epidermal and Blood Vessel Barrier Functions of Glucosylceramides and Digalactosyldiacylglycerols Isolated from Yellow Strawberry Guava

by Akari Yoneda, Shogo Takeda, Kenchi Miyasaka, Yoshiaki Manse, Toshio Morikawa and Hiroshi Shimoda

Processes 2024, 12(7), 1421; https://doi.org/10.3390/pr12071421 - 8 Jul 2024

Viewed by 904

Abstract

Strawberry guava is the fruit of Psidium littorale, which grows in tropical regions. Few studies have examined the hydrophobic compounds and biological activities of this fruit. Therefore, we purified lipophilic compounds of strawberry guava and examined their effects on epidermal and blood [...] Read more.

Strawberry guava is the fruit of Psidium littorale, which grows in tropical regions. Few studies have examined the hydrophobic compounds and biological activities of this fruit. Therefore, we purified lipophilic compounds of strawberry guava and examined their effects on epidermal and blood vessel barrier functions as well as their anti-melanogenic activity. Lipophilic compounds were isolated by silica gel column chromatography followed by reversed-phase HPLC with MeOH from an EtOH extract of the fruit. Isolated compounds were identified by comparing NMR and MS spectra with those of reference values. The effects of these compounds on epidermal barrier function were evaluated by measuring transepidermal water loss (TEWL) using reconstructed human epidermal keratinocytes (RHEKs). Blood vessel barrier function was examined using dye permeability through human umbilical vein endothelial cell (HUVEC) layers. Anti-melanogenic activity was assessed by theophylline-induced melanogenesis in B16 melanoma cells. We isolated six glucosylceramides (GlcCers) and three digalactosyldiacylglycerols (DGDGs). Only GlcCer[t18:1(8Z)/23:0] significantly lowered TEWL in RHEKs, while GlcCer[t18:1(8Z)/24:0] induced a slight reduction. Regarding the permeability of the HUVEC layer, GlcCer[d18:2(4E,8Z)/16:0] and DGDG (1,2-dilinolenoyl-3-digalactosylglycerol) significantly suppressed dye permeability and this effect was accompanied by the expression of VE-cadherin, which facilitates cell-to-cell adhesion. GlcCers and DGDGs did not exhibit anti-melanogenic activity. Therefore, strawberry guava containing specific GlcCers and DGDGs may promote epidermal and blood vessel barrier functions. Full article

(This article belongs to the Topic Purification of Plant Extracts)

►▼ Show Figures

Graphical abstract

attachment

Supplementary material:
Supplementary File 1 (ZIP, 616 KiB)

16 pages, 3519 KiB  

Open AccessArticle

Development of Pure Certified Reference Material of Cannabidiol

by Congcong Yu, Ruihan Long, Feng Cao, Xinying Zhao, Tao Lan and Dunming Xu

Molecules 2024, 29(5), 921; https://doi.org/10.3390/molecules29050921 - 20 Feb 2024

Cited by 1 | Viewed by 1766

Abstract

Cannabidiol (CBD) is the major functional component in hemp and has a broad range of pharmacological applications, such as analgesic, anti-epileptic, anti-anxiety, etc. Currently, CBD is widely used in pharmaceuticals, cosmetics, and food. To ensure the quality and safety of the products containing [...] Read more.

Cannabidiol (CBD) is the major functional component in hemp and has a broad range of pharmacological applications, such as analgesic, anti-epileptic, anti-anxiety, etc. Currently, CBD is widely used in pharmaceuticals, cosmetics, and food. To ensure the quality and safety of the products containing CBD, more and more related sample testing is being conducted, and the demand for CBD-certified reference material (CRM) has also sharply increased. However, there is currently a lack of relevant reference materials. In this paper, a simple method for preparing CBD CRM was established based on preparative liquid chromatography using crude hemp extract as a raw material. A qualitative analysis of CBD was performed using techniques such as ultraviolet absorption spectroscopy (UV), infrared spectroscopy (IR), mass spectrometry (MS), nuclear magnetic resonance spectroscopy (NMR), and differential scanning calorimetry (DSC). High-performance liquid chromatography (HPLC) was used for the homogeneity and stability tests, and the data were analyzed using an F-test and a T-test, respectively. Then, eight qualified laboratories were chosen for the determination of a certified value using HPLC. The results show that the CBD CRM had excellent homogeneity and good stability for 18 months. The certified value was 99.57%, with an expanded uncertainty of 0.24% (p = 0.95, k = 2). The developed CBD CRM can be used for the detection and quality control of cannabidiol products. Full article

(This article belongs to the Topic Purification of Plant Extracts)

►▼ Show Figures

Graphical abstract

15 pages, 4419 KiB  

Open AccessArticle

Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Equisetum via Response Surface Methodology Based on Antioxidant Activity

by Hongmei Yin, Yuanli Zhang, Tingting Hu, Wen Li, Yang Deng, Xun Wang, Huaqiao Tang, Ling Zhao and Guangwen Yan

Processes 2023, 11(7), 1978; https://doi.org/10.3390/pr11071978 - 30 Jun 2023

Cited by 1 | Viewed by 1703

Abstract

Flavonoids are one of the most essential compounds in various plants. This study used the cellulase-assisted method to extract flavonoid-enriched antioxidants from Equisetum arvense L. (EAL). According to a three-factor and three-level central composite design, the response surface methodology was used to obtain [...] Read more.

Flavonoids are one of the most essential compounds in various plants. This study used the cellulase-assisted method to extract flavonoid-enriched antioxidants from Equisetum arvense L. (EAL). According to a three-factor and three-level central composite design, the response surface methodology was used to obtain the maximum total flavonoid yield, thereby providing the most potent antioxidant activity of EAL extracts. The results indicated that the optimal condition for the total flavonoid extraction was 0.52% (base substrate) enzyme concentration and enzymolysis for 50.58 min at 49.03 °C. The extraction rate of flavonoids from horsetail reached 4.88 mg/g under these optimal conditions. The yield was 51.23% higher than that from the traditional solid–liquid extraction method. The highest DPPH-scavenging ability of the extracts obtained under 0.50% enzyme concentration and enzymolysis for 49.41 min at 46.59 °C was 77.36%. HPLC results revealed that the antioxidant substances had diverse flavonoids. Thus, the extraction condition was consistent for antioxidant activity and total flavonoids. Therefore, the current study provided an efficient method to extract flavonoid compounds from EAL, facilitating technical support for utilizing the plant. Full article

(This article belongs to the Topic Purification of Plant Extracts)

►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less

Select all

Export citation of selected articles as:

Plain Text BibTeX BibTeX (without abstracts) Endnote Endnote (without abstracts) Tab-delimited RIS

 

Error

Oops... you haven't selected anything for export.

Ok

clear

Displaying articles 1-3