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Article

Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast

by
Musrat Zahan Surovy
1,2,*,
Shahinoor Rahman
3,
Michael Rostás
3,
Tofazzal Islam
2 and
Andreas von Tiedemann
1,*
1
Division of Plant Pathology and Crop Protection, Department of Crop Sciences, Georg-August-University of Goettingen, Grisebachstrasse 6, 37077 Goettingen, Germany
2
Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh
3
Division of Agricultural Entomology, Department of Crop Sciences, Georg-August-University of Goettingen, Grisebachstrasse 6, 37077 Goettingen, Germany
*
Authors to whom correspondence should be addressed.
Microorganisms 2023, 11(5), 1291; https://doi.org/10.3390/microorganisms11051291
Submission received: 16 April 2023 / Revised: 2 May 2023 / Accepted: 4 May 2023 / Published: 16 May 2023
(This article belongs to the Special Issue Antifungal Activity of Bacillus Species against Plant Pathogens)
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Abstract

:
The Magnaporthe oryzae Triticum (MoT) pathotype is the causal agent of wheat blast, which has caused significant economic losses and threatens wheat production in South America, Asia, and Africa. Three bacterial strains from rice and wheat seeds (B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) were used to explore the antifungal effects of volatile organic compounds (VOCs) of Bacillus spp. as a potential biocontrol mechanism against MoT. All bacterial treatments significantly inhibited both the mycelial growth and sporulation of MoT in vitro. We found that this inhibition was caused by Bacillus VOCs in a dose-dependent manner. In addition, biocontrol assays using detached wheat leaves infected with MoT showed reduced leaf lesions and sporulation compared to the untreated control. VOCs from B. velezensis BTS-4 alone or a consortium (mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) of treatments consistently suppressed MoT in vitro and in vivo. Compared to the untreated control, VOCs from BTS-4 and the Bacillus consortium reduced MoT lesions in vivo by 85% and 81.25%, respectively. A total of thirty-nine VOCs (from nine different VOC groups) from four Bacillus treatments were identified by gas chromatography–mass spectrometry (GC–MS), of which 11 were produced in all Bacillus treatments. Alcohols, fatty acids, ketones, aldehydes, and S-containing compounds were detected in all four bacterial treatments. In vitro assays using pure VOCs revealed that hexanoic acid, 2-methylbutanoic acid, and phenylethyl alcohol are potential VOCs emitted by Bacillus spp. that are suppressive for MoT. The minimum inhibitory concentrations for MoT sporulation were 250 mM for phenylethyl alcohol and 500 mM for 2-methylbutanoic acid and hexanoic acid. Therefore, our results indicate that VOCs from Bacillus spp. are effective compounds to suppress the growth and sporulation of MoT. Understanding the MoT sporulation reduction mechanisms exerted by Bacillus VOCs may provide novel options to manage the further spread of wheat blast by spores.

1. Introduction

Wheat is a major cereal crop worldwide [1], and, according to the United States Department of Agriculture (USDA), 779.03 million tons of wheat were produced globally in 2021 [2]. Wheat blast is a devastating fungal disease that is caused by the Magnaporthe oryzae Triticum (MoT) pathotype [3,4,5]. It can cause significant reductions in wheat yield and grain quality [6,7]. Wheat blast first emerged in Brazil in 1985 and then gradually spread to Argentina, Bolivia, and Paraguay [5,6,8]. Outbreaks of wheat blast in Bangladesh and Zambia in recent years confirmed its expansive potential [9,10]. Recently, it has also been recognized as a potential future threat to European wheat production [11].
Fungicides are commonly used to control blast and other ear-related wheat diseases [12]. However, the failure of fungicides to fully control MoT has led to repetitive applications, increasing the selection pressure on the pathogen to develop resistance [13,14]. The emergence of new fungicide-resistant strains of MoT severely threatens wheat production [7,15], which is why the use of resistant cultivars is an important strategy to control wheat blast. However, MoT can break cultivar resistance, and, so far, no cultivars are entirely resistant to MoT [5,16].
Biological control is a potential alternative to manage plant pathogens [17,18,19]. Over the past three decades, multidisciplinary research in biocontrol has investigated effective strategies to control wheat pathogens [20]. The mechanisms exerted by biological control agents (BCAs) include direct (hyperparasitism, antibiosis) and indirect (competition, induction of host plant resistance) modes of action [19,21,22,23]. Direct suppression of target fungi by BCAs can be achieved by the production of antibiotics, volatile organic compounds (VOCs), or other bioactive secondary metabolites [21] that have an antagonistic effect on pathogens [19]. The inhibitory activity and biocontrol potential of some pure microbial non-volatile secondary metabolites against the wheat blast fungus MoT have been reported recently [24,25,26,27].
Bacterial VOCs are low-molecular-weight (<300 Da) [28,29], hydrophobic, low-toxicity, and naturally occurring substances [30]. They are diffusible in the environment and have been shown to be effective in biofumigation [31,32]. Recent discoveries of antifungal bacterial volatiles have triggered research interest in exploring the potential use of VOCs in the control of fungal pathogens. The efficiency of bacterial VOCs depends on the adaptability of bacteria to diverse environmental conditions, nutritional properties, and bacterial colonization patterns in specific hosts [21,33]. To date, relatively few bacterial biocontrol agents have been employed in agriculture due to their low field efficacy, safety concerns, or issues related to international market trading [34]. VOCs from Pseudomonas [30,35], Bacillus [36,37], Burkholderia [38,39], and Serratia [40] have been reported for their active antifungal activity. VOCs from Trichoderma fungi [41] are effective against wheat crown and root rot, and those from Bacillus spp. against wheat fusarium crown and root rot [42] and fusarium head blight [43].
The potential antagonistic activity of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A against MoT was evaluated previously through in vitro and in vivo screening. These studies decoded the bacterial genes responsible for antagonism and induced systemic resistance (ISR) in host plants by whole-genome sequencing [44,45]. Additionally, the gene responsible for acetoin, a volatile organic compound, was confirmed in the tested Bacillus spp. However, it has been reported that Bacillus spp. can produce diversified antifungal volatile organic compounds (VOCs) to suppress phytopathogenic growth [36,37,42,43]. Thus, it is essential to identify all potential VOCs from our selected Bacillus spp. and their mechanisms of action to suppress the growth of MoT. Therefore, to further understand the mechanisms underlying the antagonistic action of these bacterial VOCs, this study investigates the role of Bacillus VOCs in the suppression of MoT without direct contact. More specifically, the objectives of the current study were to (i) investigate the effects of Bacillus VOCs on the mycelial growth and sporulation of MoT in vitro; (ii) demonstrate the suppressive effects of Bacillus volatiles against wheat leaf infection with MoT; (iii) identify and characterize Bacillus VOCs through GC–MS; and (iv) confirm the suppressive effects of selected pure VOCs against MoT.

2. Materials and Methods

2.1. Bacterial Strains and Culture Conditions

Three bacterial strains, B. subtilis BTS-3 (NCBI accession WOVJ00000000), B. velezensis BTS-4 (NCBI accession WOVK00000000), and B. velezensis BTLK6A (NCBI accession WOYD00000000), were used in this study [23]. BTS-3 and BTS-4 bacterial strains were isolated from the ‘Rangabinni’ rice seeds and BTLK6A from the ‘Kanchan’ wheat seeds of Bangladesh [23,44,46,47]. All Bacillus strains were stored as pure cultures in 20% glycerol at −20 °C. Bacterial strains were streaked into Petri dishes (90 mm) containing ca. 20 mL Luria broth agar (LBA: 10 g tryptone, 5 g yeast extract, 10 g NaCl, 15 g agar, 1000 mL H2O) and incubated for 24–48 h at 25 °C. Then, three single colonies were inoculated into a 50 mL Erlenmeyer flask containing 25 mL LB (10 g tryptone, 10 g NaCl, 5 g yeast extract, 1000 mL H2O) and incubated on a rotary shaker (100 rpm) for 24–48 h at 25 °C. After incubation, the bacterial cultures were transferred to 2 mL Eppendorf tubes and centrifuged for 10 min (13,000 rpm). The supernatant was discarded and the bacterial sediment washed (3 times) with sterilized distilled water (SDW). The bacterial densities were then adjusted (1 × 109, 1 × 108, and 1 × 107 CFU/mL) for further use and stored in 20% glycerol at −80 °C for long-term preservation.

2.2. Fungal Strain and Culture Conditions

MoT fungal pathogen BTGP 6(f) was isolated from blast-infected wheat ear [48] and grown on V8 agar (V8A) following the protocol described by Surovy et al. [49]. The conidial suspension was prepared from 7-d-old MoT cultures by adding 0.01% sterile Tween 20 solution (10 mL) per plate. The suspension was filtered through a two-layer cheesecloth, and the conidial density was adjusted (1 × 105 conidia/mL) using a hemocytometer (Fuchs-Rosenthal, 0.0625 mm2).

2.3. Volatile Assays

2.3.1. Bi-Partitioned Petri Dish Assay

Bi-partitioned Petri dishes (90 mm diameter) were used to assess the potential of Bacillus VOCs against MoT. LBA medium (10 mL) was poured into one side, and 10 mL of V8A was poured into the other side of the Petri dishes. The bacterial suspension (100 µL) was pipetted in LBA, spread with a glass spreader, and incubated for 24 h at 25 °C. Three different bacterial densities (1 × 109, 1 × 108, and 1 × 107 CFU/mL) were used for this experiment. Twenty-four hours after bacterial incubation, a 2-mm 7-d-old MoT mycelial plug was placed on the side containing V8A. The Petri dishes were tightly closed with parafilm to avoid the evaporation of bacterial VOCs and incubated under the same conditions described earlier (see Section 2.2) for 5 d. The mycelial radial growth (mm) of MoT was recorded 5 d after incubation. Subsequently, 10 mL of sterilized 0.01% Tween 20 was added per plate and MoT conidia were dislodged from mycelia using a paint brush (da Vinci, Germany; size 3/0). The conidial suspension was filtered through a two-layer cheesecloth, and conidia were counted (conidia/plate) with a hemocytometer (Fuchs-Rosenthal, 0.0625 mm2). Six replications were maintained in each experiment, and three repetitive experiments were performed.

2.3.2. Upside-Down Petri Dish Assay

Bacterial strains at different densities (1 × 109, 1 × 108, and 1 × 107 CFU/mL) were grown in Petri dishes containing LBA for 24 h at 25 °C. Twenty-four hours after bacterial incubation, 10 µL (1 × 105 conidia/mL) of MoT conidial suspension was drop-inoculated in another Petri dish containing V8A. These two plates, one containing bacteria and one MoT, were placed face-to-face on top of each other, tightly sealed with parafilm to avoid the loss of VOCs, and incubated for 5 d at 25 °C. The mycelial radial growth of MoT (mm) and the total number of MoT conidia/plate were recorded as described in Section 2.3.1. Six replications were maintained in each experiment, and three repetitive experiments were performed.

2.4. Detached Leaf Assay

Wheat cultivar BR 18 was used for the detached leaf assay. The seeds were surface-sterilized with sodium hypochlorite (3% NaOCl) for 1 min and subsequently washed (3 times) with sterilized distilled water (SDW). Treated seeds were placed in Petri dishes containing moistened filter paper. After germination, they were then sown in plastic pots (7 × 7 × 8 cm; 10 seeds per pot) containing a mixture of sand, compost, and peat (1:2:1). Plants were grown in a greenhouse maintaining a 14/10 h light-dark cycle, 25 °C (±2) temperature, and 65–70% relative humidity. At growth stage 13 (GS 13, three leaves emerged), the second leaf was cut into small pieces (ca. 2 cm) and surface-sterilized with 3% NaOCl. The extra water from the surface-sterilized leaves was removed with a sterile paper towel. Leaf pieces were then placed on water agar (15 g agar, 1000 mL H2O) containing benzimidazole (30 mg/L). Ten leaf pieces were placed in each Petri dish. The MoT conidial suspension (1 × 105 conidia/mL) was drop-inoculated (10 µL) on each leaf piece; for the control only, water (10 µL) was inoculated on each leaf instead of MoT conidial suspension. The bacterial suspension (100 µL, 1 × 109 CFU/mL) was incubated in LBA for 24 h before the preparation of leaf pieces. After incubation, freshly grown (at 25 °C) bacterial culture plates were placed open and upside-down on the Petri dishes containing leaf pieces. Plates were sealed tightly with parafilm to avoid the loss of VOCs. Five days after incubation, the lesion growth and total number of conidia in each lesion were recorded. The lesion size (cm2) was determined by using the ImageJ software (version 1.53 m). A single leaf section was placed in a 2 mL Eppendorf tube containing 1 mL water, briefly vortexed, and MoT conidia per lesion were counted using a hemocytometer (Fuchs-Rosenthal, 0.0625 mm2). Thirty leaf pieces were used for each bacterial treatment, and three repetitive experiments were performed.

2.5. Identification and Quantification of Bacillus Volatiles

Bacillus VOC collection was performed as described previously by Sarenqimuge et al. [50]. As an internal standard, 200 ng of tetralin (1,2,3,4 tetrahydronaphthalene, Sigma-Aldrich, Munich, Germany) was added to each sample before GC–MS analysis. An aliquot of 30 µL sample was transferred to another GC vial with a glass insert and placed into the tray of the GC–MS autosampler. A 2 µL sample was injected in pulsed splitless mode for analysis. The oven temperature was retained at 40 °C for 3 min and gradually increased (8 °C/min) to a final temperature of 220 °C for 10 min. Helium was used as a carrier gas (flow rate was 1.5 mL/min). A homogenous series of n-Alkenes (C7–20) was used to determine retention indices. The MassHunter instrument (Agilent Technologies: GC 7890B, MS 5977B, Santa Clara, CA, USA) was used for data processing; MSD ChemStation software with the NIST17 and Willey11 mass spectral libraries was used to tentatively identify bacterial VOCs by their mass spectra and retention indices. The identities of the ten bioactive compounds tested in Section 2.6 were confirmed by GC–MS analysis of commercially available standards. The VOC quantification was performed by comparing the peak areas of individual compounds to the peak area of the internal standard (tetralin). From each treatment, five replicates were analyzed, and LB without bacteria was used as a control.

2.6. Bioassay with Pure Volatile Compounds

Pure VOCs (Table S1) were tested against MoT at four different concentrations (5 M, 1 M, 500 mM, and 250 mM), with DMSO as a diluent. Five sterilized paper discs were glued (Tesa stick, tesa SE, Hugo-Kirchberg-Str.1, D-22848 Norderstedt) onto the Petri dish lid, and 20 µL of each pure compound was pipetted on each paper disc (total 100 µL per Petri dish). A 2 mm MoT mycelial block was placed in the center of a V8A plate and the two plates were sealed tightly to avoid the loss of VOCs. Five days after incubation, the mycelial radial growth of MoT and the total number of conidia per plate were recorded as described in Section 2.3.1.

2.7. Statistical Analysis

All data were analyzed by using linear models (LMs) in the R software (version 4.0.5, accessed 31 March 2021) integrated into R studio (version 1.2.5001, accessed 31 March 2021). The functions ‘test dispersion’ and ‘StimulatedResiduals’ of the ‘DHARMa’ package were used to test the dispersion and residuals of the models. These functions of the ‘DHARMa’ package use a simulation-based method to create readily interpretable, scaled residuals for fitted linear models. Analysis of variance (ANOVA) was calculated for normally distributed data, followed by Tukey multiple comparisons (p < 0.05), by using the ‘emmeans’ package. For non-normally distributed data sets, the Kruskal–Wallis test was performed by using the ‘kruskal.test’ function, followed by Dunn multiple comparison analyses by using the ‘FSA’ and ‘rcompanion’ packages (p < 0.05). The ‘ggplot2’ package was used to visualize bar graphs, and the ‘ggVennDiagram’ function was used to plot the number of VOCs produced in different Bacillus treatments in a Venn diagram. The ‘ComplexHeatmap’ function was used to visualize the Bacillus VOC profiles, the effects of pure VOCs on MoT mycelial growth, and MoT sporulation in a heatmap. MetaboAnalyst 5.0 [51] was used for volcano plot analysis.

3. Results

3.1. Bioassay with Volatiles

3.1.1. Effects of Bacillus VOCs on MoT Growth

Three different bacterial densities (1 × 107, 1 × 108, and 1 × 109 CFU/mL) were used in a bi-partitioned Petri dish assay to evaluate the effects of Bacillus VOCs on MoT mycelial growth. Different bacterial VOCs significantly inhibited MoT mycelial growth in a density-dependent manner (F = 161.44, p < 0.001) (Figure 1A). Compared to the control (43 mm), the highest reduction (72.5%) in mycelial growth was recorded in BTS-4 (1 × 109 CFU/mL, 11.8 mm diameter), and the lowest (3%) in BTLK6A (1 × 107 CFU/mL and 41.5 mm diameter). Inhibition of MoT mycelial growth was consistently higher at the bacterial density of 1 × 109 CFU/mL for all four Bacillus treatments (Figure 1A,B).
In addition to the assessment of MoT mycelial growth, the sporulation rate was evaluated after 5 d of bacterial treatment. Treatments with Bacillus VOCs significantly suppressed MoT sporulation (F = 23.092, p ≤ 0.001). All three densities (1 × 107, 1 × 108, and 1 × 109 CFU/mL) of BTS-3, BTS-4, and the Bacillus consortium treatments produced non-spore-forming white mycelia (100% reduction in conidia compared to control). In the case of BTLK6A, no sporulation was observed at 1 × 109 CFU/mL. However, at 1 × 107 and 1 × 108 CFU/mL densities, black/grey-colored MoT sporulating colonies were observed as in the untreated control. However, the number of conidia was comparatively less in 1 × 107 (2.37 × 105 conidia/plate, 73% reduction compared to control) and 1 × 108 CFU/mL (6.60 × 104 conidia/plate, 92% reduction compared to control) of BTLK6A treatment compared to the control (9.10 × 105 conidia/plate) (Figure 1C).

3.1.2. Effects of Bacillus VOCs against Germination of MoT Conidia

An upside-down Petri dish assay was performed to evaluate the effect of Bacillus VOCs on MoT conidia germination. All MoT conidia germinated, and mycelial growth ensued after exposure to Bacillus VOCs. However, MoT mycelial growth was very slow in the Bacillus consortium treatment (1 × 109 CFU/mL). Additionally, less intense, flat mycelial growth was observed with all BTS-4 treatments (Figure 2A). Similar to the bi-partitioned Petri dish assay, the bacterial VOCs also significantly inhibited MoT mycelial growth (developed from MoT conidia) (F = 372.63, p ≤ 0.001).
Mycelial growth reduction was higher in the upside-down Petri dish assay than the bi-partitioned Petri dish assay. The highest inhibition of mycelial growth was recorded with the treatment of the Bacillus consortium (1 × 109 CFU/mL), with radial growth of 13.2 mm, followed by BTS-4 (1 × 109 CFU/mL, 16.8 mm). The lowest reduction was documented for BTLK6A (1 × 107 CFU/mL, 42.9 mm) (Figure 2B). The highest sporulation was recorded for the control (9.73 × 105 conidia/plate). However, the complete suppression of sporulation of MoT was recorded for all bacterial treatments except for BTLK6A (1.23 × 105 conidia/plate at 1 × 107 CFU/mL, 87% reduction in sporulation compared to control) (Figure 2C).

3.2. Effects of Bacillus VOCs in Detached Leaf Assay

To investigate the capacity of Bacillus VOCs to reduce leaf infection with MoT, a detached leaf assay was performed using four different Bacillus VOC treatments (Figure 3A). In our experiment, we found that Bacillus VOCs significantly reduced the development of blast disease symptoms in detached leaves and suppressed MoT sporulation under laboratory conditions, but with varying effects. Bacillus VOCs significantly reduced the lesion size (F = 37.14, p ≤ 0.001) and MoT conidia production (F = 28.49, p ≤ 0.001) (Figure 4A). The largest lesion (0.48 cm2) was recorded in the untreated control, followed by BTLK6A, where BTLK6A VOCs reduced the lesion size by 43.75% (0.27 cm2) compared to the control. The smallest lesion was observed in BTS-4, with a >85% reduction in lesion size (0.07 cm2) compared to the control, followed by the Bacillus consortium (81.25% reduction, 0.09 cm2) and BTS-3 (72.9% reduction, 0.13 cm2). Therefore, there were no significant differences between the BTS-3, BTS-4, and Bacillus consortium treatments (Figure 3B).
MoT sporulation in VOC-treated leaf lesions was lower compared to the control. A single lesion on a control leaf segment yielded 5.6 × 104 conidia/lesion, significantly different from all other bacterial treatments. The BTS-4-treated leaf segments had the lowest number of conidia (1.9 × 103 conidia/lesion), followed by the Bacillus consortium (3.5 × 103 conidia/lesion). However, the numbers of conidia produced in BTS-4 and consortium-treated leaf lesions were not significantly different. There was no MoT sporulation in the water-treated control as there was no MoT infection present (Figure 3C).

3.3. Identification and Quantification of Bacillus Volatile Organic Compounds (VOCs)

The VOCs produced from the four different Bacillus treatments (BTS-3, BTS-4, BTLK6A, and consortium (a mixture of all three Bacillus strains)) were identified and quantified using GC–MS. Thirty-nine VOCs were identified in total, of which 11 were produced by all four bacterial treatments (Figure 4A, Table S2).
The greatest diversity of VOCs were released by BTS-4 (34), followed by the Bacillus consortium (22), and lastly by BTLK6A (12). Among the 39 VOCs, 12 unique VOCs were produced by BTS-4, two by the Bacillus consortium, and only one by BTS-3. A total of nine different classes of volatiles were identified: alkanes 7.70%, alcohols 23.07%, ketones 20.51%, fatty acids 12.82%, aldehydes 15.38%, aromatic 2.56%, N-containing 10.25%, S-containing 5.12%, and alkene compounds 2.56% (Figure 4B). Alcohol, fatty acid, ketone, S-containing, and aldehyde compounds were identified in all four Bacillus treatments. The highest number of diversified VOC classes was detected in BTS-4 (9), followed by BTS-3 (8), the consortium (7), and BTLK6A (6). The number of alcoholic VOCs was higher for BTS-4, followed by the Bacillus consortium. Fatty acid VOCs were also higher in BTS-4, followed by the BTS-3 and Bacillus consortium treatments (Figure 4B). The concentrations of bacterial VOCs produced by different treatments differed significantly. The heatmap analysis represents the VOC clustering and the relationships between different bacterial treatments (Figure 4C).
In vitro and in vivo experimental data indicated that VOCs from the BTS-4 and Bacillus consortium treatments had considerable potential to control MoT. Therefore, we investigated the relationships between the VOCs produced from the BTS-4 and consortium treatments to determine the effectiveness of BTS-4 and Bacillus consortium volatiles against MoT. Figure 5 displays the fold change (p ≤ 0.05) in VOC production from BTS-4 compared to the Bacillus consortium. In BTS-4, 21 VOCs were upregulated, 4 were down-regulated, and 9 were not significantly different from the Bacillus consortium treatment (Figure 5).

3.4. Effect of Pure VOCs on Mycelial Growth and Sporulation of MoT

From 39 identified VOCs, ten bioactive VOCs (2-methyl propionic acid, 3-methyl-1-butanol, 3-methyl butanoic acid, 2,3-butanediol, and 2-methyl butanoic acid, phenyl ethyl alcohol, hexanoic acid, 2-methyl-1-butanol, 2,5-dimethyl pyrazine, and acetoin) were tested in vitro against MoT based on the previous literature [50]. DMSO and water were used as positive controls. Of the selected compounds, four VOCs (2-methyl butanoic acid, 2-methyl propanoic acid, 2,5- dimethyl pyrazine, and 3-methyl butanoic acid) were produced by all four bacterial treatments; three (2,3-butanediol, 3-methyl-1-butanol, and phenyl ethyl alcohol) were produced by BTS-4 and the Bacillus consortium; two VOCs (2-methyl-1-butanol and acetoin) only in BTS-4; and one (hexanoic acid) in the BTS-3 and BTS-4 treatments.
The efficacy of single pure volatile compounds against MoT mycelial growth was assessed in an in vitro bioassay. Pure VOCs were used at four different concentrations (5 M, 1 M, 500 mM, and 250 mM). At 5 M, all compounds except acetoin and 2,3-butanediol inhibited the mycelial growth of MoT (Figure 6A). Among the pure compounds, hexanoic acid suppressed MoT growth up to a 500 mM concentration (Figure S1 and Table S3).
In parallel with the reduction in MoT mycelial growth, the selected pure VOCs significantly reduced sporulation from MoT mycelia in vitro (Figure S2 and Table S3). No sporulation was observed in any of the four treatments with phenylethyl alcohol (PEA). Similarly, no sporulation was recorded for hexanoic acid or 2-methylbutanoic acid up to a 500 mM concentration (Figure 6B). Figure 6A,B contain heatmaps showing the relative effects of potential VOCs on the reduction in the mycelial growth and sporulation of MoT. The lowest VOC concentration inhibitory to MoT sporulation was recorded for PEA (250 mM), followed by 2-methylbutanoic acid and hexanoic acid at 500 mM (Figure 6B).

4. Discussion

In this study, we used four bacterial treatments (BTS-3, BTS-4, BTLK6A, and a consortium of Bacillus spp.) to assess the effects of Bacillus spp. VOCs to control an emerging fungal pathogen, the M. oryzae Triticum (MoT) pathotype. All Bacillus treatments produced diverse VOCs and exhibited strong antagonism against MoT, by suppressing mycelial growth and sporulation in vitro. It is well documented that Bacillus VOCs exert antifungal activity against various phytopathogens [52,53]. Additionally, some studies have reported that the volatiles from B. megaterium [54], endophytic Chryseobacterium [37], and Pseudomonas sp. [55] suppress the mycelial growth of the rice blast pathogen (M. oryzae Oryzae pathotype). However, so far, no information has been made available about the suppression of MoT mediated by Bacillus volatiles. To the best of our knowledge, this is the first report on Bacillus VOCs significantly inhibiting the mycelial growth of this important pathogen.
It has been previously reported that bacterial consortia are more effective at controlling certain fungal pathogens than single bacterial strains [56,57]. In this study, B. velezensis BTS-4 and the Bacillus consortium performed better than the other Bacillus treatments in suppressing MoT mycelial growth and conidial germination in vitro. Additionally, the densitiy of Bacillus spp. had a significant positive correlation with MoT inhibition. At 1 × 109 CFU/mL, the MoT inhibition rate was higher than at 1 × 107 and 1 × 108 CFU/mL. The higher densitiy of Bacillus spp. led to more Bacillus colony growth, higher VOC production, and a significant reduction in the growth and sporulation of MoT. B. velezensis can inhibit the growth of Colletotrichum gloeosporioides at a density of 1 × 107 CFU/mL [52] and S. sclerotiorum at a density of 1 × 108 CFU/mL [58]. Furthermore, B. subtilis has been documented to control Alternaria solani at a density of 1 × 108 CFU/mL [59], and B. amyloliquefaciens VOCs can suppress Fusarium oxysporum f. sp. cubense in vitro also at a density of 1 × 108 CFU/mL [60].
These findings suggest that the VOCs from Bacillus spp. may lead to the functional degradation of MoT mycelia and thus suppress sporulation from MoT mycelia. Deformed hyphae with vacuolation, excessive branching, the degeneration of hyphal cells, or combinations of excessive branching with vacuolation were recorded (Figure S3). Likewise, the VOCs from B. velezensis and B. atrophaeus also cause vacuolation and cavities in the mycelial cytoplasm of B. cinerea [58], and VOCs of B. subtilis may cause expanded, uneven, flaccid hyphae and the suppression of A. solani sporulation [59].
Furthermore, Bacillus VOCs significantly reduced the leaf blast lesion size and further MoT sporulation from the lesions in an in vivo detached leaf assay. Conidia are the main dispersal units of MoT epidemiology. Reduced or no conidia formation will result in reduced wheat infection by MoT. Therefore, understanding the MoT sporulation reduction mechanisms of Bacillus VOCs is the first step in controlling MoT epidemics. Earlier reports reveal that the volatiles of Bacillus spp. May reduce the lesion size and sporulation of A. solani in potato leaves [59], as well as the sporulation of Sclerotinia sclerotiorum on tomato, tobacco, and soybean leaves in vivo [61]. However, our results confirm that exposure to Bacillus VOCs does not entirely prevent MoT infection or sporulation but rather slows down the development of blast symptoms compared to the untreated control. Bacillus VOCs also significantly reduced blast lesion development in the detached spike assay (data not shown).
GC–MS was used to determine the active VOCs from different Bacillus treatments. Thirty-nine VOCs were identified in total, of which 11 VOCs were produced in all four Bacillus treatments. The emitted bacterial VOCs were alcohols, alkenes, alkynes, ketones, aldehydes, fatty acids, aromatic, N-containing, and S-containing compounds. The VOCs identified in our analysis have demonstrated broader antifungal activity against phytopathogens. The mixture of alcoholic volatiles 2-methyl-1-butanol and 3-methyl-1-butanol was very effective in suppressing the growth of Phyllosticta citricarpa [62] and Aspergillus flavus [63], and 2-ethyl-1-hexanol strongly inhibited the growth of Colletotricum acutatum [64] and B. cinerea [65].
The compound 6-methyl-2-heptanone disrupts mycelial integrity, collapses conidial vesicles, and downregulates the conidial germination gene of pathogenic fungus [66]. The S-containing volatile compound benzothiazole inhibits cystospore germination and the mycelial growth of Phythophthora parasitica var. nicotianae [67]. Hexanal, an aldehyde group volatile, induces systemic resistance in mango plants by inducing defense-related enzymes (phenylalanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), superoxide dismutase (SOD), and catalase (CAT)), thus significantly reducing Lasiodiplodia theobromae infection [68].
In our study, ten VOCs were selected to test their potential in inhibiting MoT based on their reported bioactivity in the literature. All selected VOCs except acetoin and 2,3-butanediol significantly reduced MoT mycelial growth and sporulation. Acetoin and 2,3-butanediol play a role in inducing systemic resistance in plants [69,70] and do not seem to be directly involved in the suppression of MoT mycelial growth and sporulation. Meanwhile, 2,5-dimethyl pyrazine stopped MoT sporulation at a 1 M concentration and has also been cited to control Sclerotinia sp., Pythium sp., Rhizoctonia sp. [71], and Anthracnose sp. [72]. The activity of 2-methyl propanoic acid against MoT was not promising; although it effectively controls rubber white root rot disease, it negatively affects seedling growth [73].
Hexanoic acid, phenylethyl alcohol, and 2-methyl butanoic acid potentially inhibited the mycelial growth and sporulation of MoT. Phenylethyl alcohol slows phytopathogenic growth by inhibiting the synthesis of RNA, DNA, and protein and upregulates genes related to the phagosome, peroxisome, proteasome, and autophagy [74]. Considering this information, it can be deduced that the phenylethyl alcohol first causes MoT mycelial alternations and later induces autophagy, triggering programmed cell death. Fatty acids have also been reported to exert inhibitory activity against some fungal pathogens, but saturated fatty acids have robust antifungal activity compared to other fatty acids [75]. This study found that hexanoic acid (a saturated fatty acid) inhibited mycelial growth and sporulation up to 500 mM. It has been documented that the minimum inhibitory concentrations (MICs) of hexanoic acid against Micosporum gypseum range from 0.02 to 75 µg/mL [76]. At a concentration of 10 mM, Candida albicans growth is inhibited by hexanoic acid through changes in intracellular hydrostatic pressure and subsequent disruption of the cell plasma membrane [77]. Additionally, hexanoic acid enhances plant jasmonic acid (JA) signaling and induces callose deposition during fungal infection [78].
Organic fertilizers promoted the growth of B. amyloliquefaciens, induced the release of 2-nonanone and nonanal, and suppressed R. solanacearum [79]. The encapsulation of Bacillus VOCs might be an effective way to use bacterial VOCs under field conditions; thus, it facilitates the slow and steady release of volatiles. Effective control of MoT using bacterial VOCs requires more detailed studies considering field environmental conditions and compatability with other control strategies. Therefore, our study suggests that Bacillus VOCs are potential biologicals to suppress MoT, with fundamental and practical implications for wheat production through reducing the severity of wheat blast. As Bacillus spp. are rich in the production of both volatile and non-volatile antimicrobial compounds [47], further studies are warranted to identify non-volatile antimicrobial secondary metabolites from the investigated Bacillus spp. that might work together to effectively control wheat blast. Field evaluation of wheat blast suppression by these Bacillus and their metabolites is required before recommending them for practical application in the biorational management of wheat blast.

5. Conclusions

Bacillus produces diverse antifungal volatile organic compounds (VOCs) that are able to suppress the growth and sporulation of MoT conidia in vitro and in vivo. Wheat blast is mainly caused by infections initiated by MoT conidia, and the suppression of MoT sporulation may have practical relevance and fundamental implications in reducing wheat blast severity.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/microorganisms11051291/s1. Figure S1. Assessment of pure VOCs on MoT mycelial growth in upside-down Petri dish assay in vitro; Figure S2. Assessment of pure VOCs on sporulation of MoT in upside-down Petri dish assay in vitro; Figure S3. Alternation of MoT mycelial morphology of representative samples caused by antifungal VOCs from Bacillus spp.; Table S1: Details of purchased pure volatile compounds; Table S2: Identification and quantification of bacterial VOCs; Table S3: Effect of pure VOCs to suppress MoT in vitro.

Author Contributions

Processed samples, planned experiments, collected data, designed and performed data analysis, visualized data, and wrote original draft, M.Z.S.; performed volatile extraction and analyzed and visualized volatile data, M.Z.S. and S.R.; coordinated, designed volatile extraction experiments, and revised the paper, M.R.; conceived the idea, provided bacterial and fungal strains, and reviewed the paper, T.I.; conceived and coordinated the project and reviewed and edited the paper, A.v.T. All authors have read and agreed to the published version of the manuscript.

Funding

M.Z.S. received a scholarship (personal reference no. 91730381) from the German academic exchange service (DAAD) to pursue her PhD degree at the Georg-August University of Goettingen, Germany. The Division of Plant Pathology and Crop Protection and the University Library (SUB) of the Georg-August University of Goettingen, Germany, funded the lab consumables and open-access publication fees.

Institutional Review Board Statement

No animals and/or human participants were involved in this research.

Informed Consent Statement

This manuscript has not been published and is not under consideration by any other journals. We have read and understood the journal policies and believe that the manuscript and study do not violate any of these. All authors have been actively and personally involved in the work leading to the manuscript and hold themselves individually and jointly responsible for its content. All authors have agreed to this submission.

Data Availability Statement

All data supporting the results are included in the manuscript.

Acknowledgments

We would like to thank Birger Koopmann, Dipali Rani Gupta, and Nur Uddin Mahmud for processing material transfer (bacterial and fungal strains) and Sarenqimuge Sarenqimuge for her suggestions and support during volatile experiments. We also acknowledge the efforts contributed by the Crop Protection Master’s students Bishnu Prasad Marahatta and Youssef Beik Saade for their preliminary work on MoT and Bacillus strains. The Institute of Biotechnology and Genetic Engineering (IBGE), Bangladesh, has provided authorization and information for the study of MoT and bacterial strains for scientific activities to the Division of Plant Pathology and Crop Protection, Georg-August University of Goettingen, Germany.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Awika, J.M. Major cereal grains production and use around the world. In Advances in Cereal Science: Implications to Food Processing and Health Promotion; Awika, J.M., Piironen, V., Bean, S., Eds.; American Chemical Society, ACS Symposium Series 1089: Washington, DC, USA, 2011; Volume 1089, pp. 1–13. [Google Scholar] [CrossRef]
  2. World Agricultural Production.com. Available online: www.worldagriculturalproduction.com/crops/wheat.aspx (accessed on 12 April 2023).
  3. Cruz, C.D.; Valent, B. Wheat blast disease: Danger on the move. Trop. Plant Pathol. 2017, 42, 210–222. [Google Scholar] [CrossRef]
  4. Ceresini, P.C.; Castroagudín, V.L.; Rodrigues, F.; Rios, J.A.; Aucique-Pérez, C.E.; Moreira, S.I.; Croll, D.; Alves, E.; de Carvalho, G.; Maciel, J.L.N.; et al. Wheat blast: From its origins in South America to its emergence as a global threat. Mol. Plant Pathol. 2018, 20, 155–172. [Google Scholar] [CrossRef] [PubMed]
  5. Islam, T.; Ansary, M.W.R.; Rahman, M.M. Magnaporthe oryzae and its pathotypes: A potential plant pandemic threat to global food security. In Plant Relationships; Scott, B., Mesarich, C., Eds.; Springer: Cham, Switzerland, 2023; Volume 5, pp. 425–462. [Google Scholar] [CrossRef]
  6. Islam, M.T.; Kim, K.-H.; Choi, J. Wheat Blast in Bangladesh: The Current Situation and Future Impacts. Plant Pathol. J. 2019, 35, 1–10. [Google Scholar] [CrossRef] [PubMed]
  7. Islam, M.T.; Gupta, D.R.; Hossain, A.; Roy, K.K.; He, X.; Kabir, M.R.; Singh, P.K.; Khan, A.R.; Rahman, M.; Wang, G.-L. Wheat blast: A new threat to food security. Phytopathol. Res. 2020, 2, 1–13. [Google Scholar] [CrossRef]
  8. Islam, M.T.; Croll, D.; Gladieux, P.; Soanes, D.M.; Persoons, A.; Bhattacharjee, P.; Hossain, S.; Gupta, D.R.; Rahman, M.; Mahboob, M.G.; et al. Emergence of wheat blast in Bangladesh was caused by a South American lineage of Magnaporthe oryzae. BMC Biol. 2016, 14, 84. [Google Scholar] [CrossRef]
  9. Tembo, B.; Mulenga, R.M.; Sichilima, S.; M’siska, K.K.; Mwale, M.; Chikoti, P.C.; Singh, P.K.; He, X.; Pedley, K.F.; Peterson, G.L.; et al. Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia. PLoS ONE 2020, 15, e0238724. [Google Scholar] [CrossRef]
  10. Latorre, S.M.; Were, V.M.; Foster, A.J.; Langner, T.; Malmgren, A.; Harant, A.; Asuke, S.; Reyes-Avila, S.; Gupta, D.R.; Jensen, C.; et al. Genomic surveillance uncovers a pandemic clonal lineage of the wheat blast fungus. PLoS Biol. 2023, 21, e3002052. [Google Scholar] [CrossRef]
  11. Barragan, A.C.; Latorre, S.M.; Mock, P.G.; Harant, A.; Win, J.; Malmgren, A.; Burbano, H.A.; Kamoun, S.; Langer, T. Wild grass strains of Magnaporthe (Syn. Pyricularia) spp. from Germany can cause blast disease on cereal crops. bioRxiv 2022. [Google Scholar] [CrossRef]
  12. Pagani, A.P.S.; Dianese, A.C.; Café-Filho, A.C. Management of wheat blast with synthetic fungicides, partial resistance and silicate and phosphite minerals. Phytoparasitica 2014, 42, 609–617. [Google Scholar] [CrossRef]
  13. Poloni, N.M.; Carvalho, G.; Vicentini, S.N.C.; Dorigan, A.F.; Maciel, J.L.N.; McDonald, B.A.; Moreira, S.I.; Hawkins, N.; Fraaije, B.A.; Kelly, D.E.K.; et al. Widespread distribution of resistance to triazole fungicides in Brazilian populations of the wheat blast pathogen. Plant Pathol. 2021, 70, 436–448. [Google Scholar] [CrossRef]
  14. Corkley, I.; Fraaije, B.; Hawkins, N. Fungicides resistance management: Maximizing the effective life of plant protection products. Plant Pathol. 2021, 71, 150–169. [Google Scholar] [CrossRef]
  15. Castroagudín, V.L.; Ceresini, P.C.; de Oliveira, S.C.; Reges, J.T.A.; Maciel, J.L.N.; Bonato, A.L.V.; Dorigan, A.F.; McDonald, B.A. Resistance to QoI fungicides is widespread in Brazilian populations of the wheat blast pathogen Magnaporthe oryzae. Phytopathology 2015, 105, 284–294. [Google Scholar] [CrossRef] [PubMed]
  16. Ceresini, P.C.; Castroagudin, V.L.; Rodrigues, F.A.; Rios, J.A.; Eduardo Aucique-Pérez, C.; Moreira, S.I.; Alves, E.; Croll, D.; Maciel, J.L.N. Wheat blast: Past, present, and future. Annu. Rev. Phytopathol. 2018, 56, 427–456. [Google Scholar] [CrossRef] [PubMed]
  17. Chet, I.; Inbar, J. Biological control of fungal pathogens. Appl. Biochem. Biotechnol. 1994, 48, 37–43. [Google Scholar] [CrossRef]
  18. Raaijmakers, J.M.; Mazzola, M. Diversity and natural functions of antibiotics produced by beneficial and plant pathogenic bacteria. Annu. Rev. Phytopathol. 2012, 50, 403–424. [Google Scholar] [CrossRef]
  19. Chakraborty, M.; Mahmud, N.U.; Ullah, C.; Rahman, M.; Islam, T. Biological and biorational management of blast diseases in cereals caused by Magnaporthe oryzae. Crit. Rev. Biotechnol. 2021, 18, 1–29. [Google Scholar] [CrossRef]
  20. Pal, K.K.; McSpadden Gardener, B. Biological control of plant pathogens. Plant Health Instr. 2006, 2, 1. [Google Scholar] [CrossRef]
  21. Köhl, J.; Kolnaar, R.; Ravensberg, W.J. Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. Front. Plant Sci. 2019, 10, 845. [Google Scholar] [CrossRef]
  22. Malik, M.S.; Haider, S.; Rehman, A.; Rehman, S.U.; Jamil, M.; Naz, I.; Anees, M. Biological control of fungal pathogens of tomato (Lycopersicon esculentum) by chitinolytic bacterial strains. J. Basic Microbiol. 2022, 62, 48–62. [Google Scholar] [CrossRef]
  23. Surovy, M.Z.; Rahman, S.; Dame, Z.T.; Islam, T. Discovery of bioactive natural products from Bacillus Species: Chemistry, biosynthesis and biological activities. In Bacilli in Agrobiotechnology. Bacilli in Climate Resilient Agriculture and Bioprospecting; Islam, M.T., Rahman, M., Pandey, P., Eds.; Springer: Cham, Switzerland, 2022; pp. 47–87. [Google Scholar] [CrossRef]
  24. Chakraborty, M.; Mahmud, N.U.; Muzahid, A.N.M.; Rabby, S.F.; Islam, T. Oligomycins inhibit Magnaporthe oryzae Triticum and suppress wheat blast disease. PLoS ONE 2020, 15, e0233665. [Google Scholar] [CrossRef]
  25. Chakraborty, M.; Mahmud, N.U.; Gupta, D.R.; Tareq, F.S.; Shin, H.J.; Islam, T. Inhibitory effects of linear lipopeptides from a marine Bacillus subtilis on the wheat blast fungus Magnaporthe oryzae Triticum. Front. Microbiol. 2020, 11, 665. [Google Scholar] [CrossRef] [PubMed]
  26. Chakraborty, M.; Rabby, S.M.F.; Gupta, D.R.; Rahman, M.; Paul, S.K.; Mahmud, N.U.; Rahat, A.A.M.; Jankuloski, L.; Islam, T. Natural protein kinase inhibitors staurosporine and chelerythrine suppress wheat blast disease caused by Magnaporthe oryzae Triticum. Microorganisms 2022, 10, 1186. [Google Scholar] [CrossRef] [PubMed]
  27. Paul, S.K.; Chakraborty, M.; Rahman, M.; Gupta, D.R.; Mahmud, N.U.; Rahat, A.A.M.; Sarker, A.; Hannan, M.A.; Rahman, M.M.; Akanda, A.M.; et al. Marine natural product antimycin A suppresses wheat blast disease caused by Magnaporthe oryzae Triticum. J. Fungi 2022, 8, 618. [Google Scholar] [CrossRef] [PubMed]
  28. Heenan-Daly, D.; Coughlan, S.; Dillane, E.; Prestwich, B.D. Volatile compounds from Bacillus, Serratia, and Pseudomonas promote growth and alter the transcriptional landscape of Solanum tuberosum in a passively ventilated growth system. Front. Microbiol. 2021, 12, 628437. [Google Scholar] [CrossRef]
  29. Fincheira, P.; Quiroz, A.; Tortella, G.; Diez, M.C.; Rubilar, O. Current advances in plant-microbe communication via volatile organic compounds as an innovative strategy to improve plant growth. Microbiol. Res. 2021, 247, 126726. [Google Scholar] [CrossRef]
  30. Schulz-Bohm, K.; Martin-Sánchez, L.; Garbeva, P. Microbial volatiles: Small molecules with an important role in intra and inter-kingdom interactions. Front. Microbiol. 2017, 8, 2484. [Google Scholar] [CrossRef]
  31. Brilli, F.; Loreto, F.; Baccelli, I. Exploiting plant volatile organic compounds (VOCs) in agriculture to improve sustainable defense strategies and productivity of crops. Front. Plant Sci. 2019, 10, 264. [Google Scholar] [CrossRef]
  32. Bui, H.X.; Desaeger, J.A. Volatile compounds as potential bio-fumigants against plant-parasitic nematodes—A mini review. J. Nematol. 2021, 53, 1–12. [Google Scholar] [CrossRef]
  33. Kandel, S.L.; Joubert, P.M.; Doty, S.L. Bacterial endophyte colonization and distribution within plants. Microorganisms. 2017, 5, 77. [Google Scholar] [CrossRef]
  34. Mawar, R.; Manjunatha, B.L.; Kumar, S. Commercialization, diffusion and adoption of bioformulations for sustainable disease management in indian arid agriculture: Prospects and challenges. Circ. Econ. Sust. 2021, 1, 1367–1385. [Google Scholar] [CrossRef]
  35. Mülner, P.; Bergna, A.; Wagner, P.; Sarajlić, D.; Gstöttenmayr, B.; Dietel, K.; Grosch, R.; Cernava, T.; Berg, G. Microbiota associated with Sclerotia of soilborne fungal pathogens–a novel source of biocontrol agents producing bioactive volatiles. Phytobiomes J. 2019, 3, 125–136. [Google Scholar] [CrossRef]
  36. Zhang, X.; Li, B.; Wang, Y.; Guo, Q.; Lu, X.; Li, S.; Ma, P. Lipopeptides, a novel protein, and volatile compounds contribute to the antifungal activity of the biocontrol agent Bacillus atrophaeus CAB-1. Appl. Microbiol. Biotechnol. 2013, 97, 9525–9534. [Google Scholar] [CrossRef] [PubMed]
  37. Kumar, M.; Charishma, K.; Sahu, K.P.; Sheoran, N.; Patel, A.; Kundu, A.; Kumar, A. Rice leaf associated Chryseobacterium species: An untapped antagonistic flavobacterium displays volatile mediated suppression of rice blast disease. Biol. Con. 2021, 1, 104703. [Google Scholar] [CrossRef]
  38. Tenorio-Salgado, S.; Tinoco, R.; Vazquez-Duhalt, R.; Caballero-Mellado, J.; Perez-Rueda, E. Identification of volatile compounds produced by the bacterium Burkholderia tropica that inhibit the growth of fungal pathogens. Bioengineered 2013, 4, 236–243. [Google Scholar] [CrossRef] [PubMed]
  39. Groenhagen, U.; Baumgartner, R.; Bailly, A.; Gardiner, A.; Eberl, L.; Schulz, S.; Weisskopf, L. Production of bioactive volatiles by different Burkholderia ambifaria strains. J. Chem. Ecol. 2013, 39, 892–906. [Google Scholar] [CrossRef] [PubMed]
  40. Minerdi, D.; Bossi, S.; Maffei, M.E.; Gullino, M.L.; Garibaldi, A. Fusarium oxysporum and its bacterial consortium promote lettuce growth and expansin A5 gene expression through microbial volatile organic compound (MVOC) emission. FEMS Microbiol. Ecol. 2011, 76, 342–351. [Google Scholar] [CrossRef]
  41. Foroutan, A. Evaluation of Trichoderma strains for biological control of wheat Fusarium foot and root rot. Rom. Agric. Res. 2013, 30, 335–342. [Google Scholar]
  42. Sasani, M.; Ahmadzadeh, M. Evaluation of antagonistic effect of several strain of Bacillus bacteria on control of crown and root rot of wheat with Fusarium pseudograminearum. Biol. Con. Pests Plant Dis. 2021, 9, 115–126. [Google Scholar] [CrossRef]
  43. Chen, L.; Heng, J.; Qin, S.; Bian, K. A comprehensive understanding of the biocontrol potential of Bacillus velezensis LM2303 against Fusarium head blight. PLoS ONE 2018, 1, e0198560. [Google Scholar] [CrossRef]
  44. Surovy, M.Z.; Gupta, D.R.; Chanclud, E.; Win, J.; Kamoun, S.; Islam, M. Plant probiotic bacteria suppress wheat blast fungus Magnaporthe oryzae Triticum pathotype. Figshare 2017, 10, m9. [Google Scholar] [CrossRef]
  45. Surovy, M.Z.; Dutta, S.; Mahmud, N.U.; Gupta, D.R.; Farhana, T.; Paul, S.K.; Joe, W.; Dunlap, C.A.; Oliva, R.; Rahmanm, M.; et al. Probiotic Bacillus species: Promising biological control agents for managing worrisome wheat blast disease. Preprints 2022. [Google Scholar] [CrossRef]
  46. Chanclud, E.; Win, J.; Malone, J.; Surovy, M.Z.; Gupta, D.R.; Islam, T.; Kamoun, S. Genome sequences of candidate wheat blast biocontrol bacteria. Figshare 2017. [Google Scholar] [CrossRef]
  47. Dutta, S.; Surovy, M.Z.; Gupta, D.R.; Mahmud, N.U.; Chanclud, E.; Win, J.; Kamoun, S.; Islam, T. Genomic analyses reveal that biocontrol of wheat blast by Bacillus spp. may be linked with production of antimicrobial compounds and induced systemic resistance in host plants. Figshare 2018, 17, 28. [Google Scholar] [CrossRef]
  48. Gupta, D.R.; Surovy, M.Z.; Mahmud, N.U.; Chakraborty, M.; Paul, S.K.; Hossain, M.S.; Bhattacharjee, P.; Mehebub, M.S.; Rani, K.; Yeasmin, R.; et al. Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype. Phytopathol. Res. 2020, 2, 30. [Google Scholar] [CrossRef]
  49. Surovy, M.Z.; Islam, T.; von Tiedemann, A. Role of seed infection for the near and far distance dissemination of wheat blast caused by Magnaporthe oryzae pathotype Triticum. Front. Microbiol. 2023, 14, 1040605. [Google Scholar] [CrossRef]
  50. Sarenqimuge, S.; Rahman, S.; Wang, Y.; von Tiedemann, A. Dormancy and germination of microsclerotia of Verticillium longisporium are regulated by soil bacteria and soil moisture levels but not by nutrients. Front. Microbiol. 2022, 13, 979218. [Google Scholar] [CrossRef]
  51. Metaboanalyst 5.0. Available online: https://www.metaboanalyst.ca/home.xhtml (accessed on 22 November 2022).
  52. Choub, V.; Won, S.J.; Ajuna, H.B.; Moon, J.H.; Choi, S.L.; Lim, H.I.; Ahn, Y.S. Antifungal activity of volatile organic compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides. Horticulturae 2022, 8, 557. [Google Scholar] [CrossRef]
  53. la Cruz-López, D.; Cruz-López, L.; Holguín-Meléndez, F.; Guillén-Navarro, G.K.; Huerta-Palacios, G. Volatile organic compounds produced by cacao endophytic bacteria and their inhibitory activity on Moniliophthora roreri. Curr. Microbiol. 2022, 79, 1. [Google Scholar] [CrossRef]
  54. Munjal, V.; Nadakkakath, A.V.; Sheoran, N.; Kundu, A.; Venugopal, V.; Subaharan, K.; Rajamma, S.; Eapen, S.J.; Kumar, A. Genotyping and identification of broad spectrum antimicrobial volatiles in black pepper rot endophytic biocontrol agent, Bacillus megaterium BP17. Biol. Cont. 2016, 92, 66–76. [Google Scholar] [CrossRef]
  55. Patel, A.; Kumar, A.; Sheoran, N.; Kumar, M.; Sahu, K.P.; Ganeshan, P.; Ashajyothi, M.; Gopalakrishnan, S.; Gogol, R. Antifungal and defense elicitor activities of pyrazines identified in endophytic Pseudomonas putida BP25 against fungal blast infected by Magnaporthe oryzae in rice. J. Plant Dis. Prot. 2021, 128, 261–272. [Google Scholar] [CrossRef]
  56. Cruz-Magalhães, V.; Guimarães, R.A.; da Silva, J.C.P.; de Faria, A.F.; Pedroso, M.P.; Campos, V.P.; Marbach, P.A.S.; de Medeiros, F.H.V.; Souza, J.T.D. The combination of two Bacillus strains suppresses Meloidogyne incognita and fungal pathogens, but does not enhance plant growth. Pest Managem. Sci. 2021, 78, 722–732. [Google Scholar] [CrossRef] [PubMed]
  57. Murty, L.D.; Shim, W.B. Streptomyces and Bacillus species utilize volatile organic compounds to impact Fusarium oxysporum f.sp. vasinfectum race 4 (Fov4) virulence and suppress Fusarium wilt in Pima cotton. BioRxiv 2021. [Google Scholar] [CrossRef]
  58. Toral, L.; Rodriguez, M.; Martinez-Checa, F.; Montano, A.; Cortés-Delgado, A.; Smolinska, A.; Llamas, I.; Sampedro, I. Identification of volatile organic compounds in extremophilic bacteria and their effective use in biocontrol of postharvest fungal phytopathogens. Front. Microbiol. 2021, 12, 773092. [Google Scholar] [CrossRef] [PubMed]
  59. Zhang, D.; Yu, S.; Yang, Y.; Zhang, J.; Zhao, D.; Pan, Y.; Fan, S.; Yang, Z.; Zhu, J. Antifungal effects of volatiles produced by Bacillus subtilis against Alternaria solani in potato. Front. Microbiol. 2020, 11, 1196. [Google Scholar] [CrossRef]
  60. Yuan, J.; Raza, W.; Shen, Q.; Huang, Q. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Appl. Environ. Microbiol. 2012, 78, 5942–5944. [Google Scholar] [CrossRef]
  61. Massawe, V.; Hanif, A.; Farzand, A.; Ochola, S.O. Volatile organic compounds of endophytic Bacillus spp. have biocontrol activity against Sclerotinia sclerotiorum. Phytopathol. 2018, 108, 1373–1385. [Google Scholar] [CrossRef]
  62. Toffano, L.; Fialho, M.B.; Pascholati, S.F. Potential of fumigation of orange fruits with volatile organic compounds produced by Saccharomyces cerevisiae to control citrus black spot disease at postharvest. Biol. Control 2017, 108, 77–82. [Google Scholar] [CrossRef]
  63. Jaibangyang, S.; Nasanit, R.; Limtong, S. Effects of temperature and relative humidity on aflatoxin B1 reduction in corn grains and antagonistic activities against aflatoxin-producing Aspergillus flavus by a volatile organic compound-producing yeast, Kwoniella heveanensis DMKU-CE82. BioControl 2021, 66, 433–443. [Google Scholar] [CrossRef]
  64. Che, J.; Liu, B.; Liu, G.; Chen, Q.; Lan, J. Volatile organic compounds produced by Lysinibacillus sp. FJAT-4748 possess antifungal activity against Colletotrichum acutatum. Biocon. Sci. Technol. 2017, 27, 1349–1362. [Google Scholar] [CrossRef]
  65. Huang, R.; Che, H.J.; Zhang, J.; Yang, L.; Jiang, D.H.; Li, G.Q. Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberrx fruits. Biol. Con. 2012, 62, 53–63. [Google Scholar] [CrossRef]
  66. Zhang, D.; Qiang, R.; Zhao, J.; Zhang, J.; Cheng, J.; Zhao, D.; Fan, Y.; Yang, Z.; Zhu, J. Mechanism of a volatile organic compound (6-Methyl-2-Heptanone) emitted from Bacillus subtilis ZD01 against Alternaria solani in potato. Front. Microbiol. 2021, 12, 808337. [Google Scholar] [CrossRef] [PubMed]
  67. Fang, Y.; Zhang, L.; Jiao, Y.; Liao, J.; Luo, L.; Ji, S.; Li, J.; Dai, K.; Zhu, S.; Yang, M. Tobacco rotated with rapeseed for soil-borne Phytophthora pathogen biocontrol mediated by rapeseed root exudates. Front. Microbiol. 2016, 7, 894. [Google Scholar] [CrossRef] [PubMed]
  68. Seethapathy, P.; Gurudevan, T.; Subramanian, K.S.; Kuppusamy, P. Bacterial antagonists and hexanal-induced systemic resistance of mango fruits against Lasiodiplodia theobromae causing stem-end rot. J. Plant Interact. 2016, 11, 158–166. [Google Scholar] [CrossRef]
  69. Wu, L.; Li, X.; Ma, L.; Borriss, R.; Wu, Z.; Gao, X. Acetoin and 2,3-butanediol from Bacillus amyloliquefaciens induce stomatal closure in Arabidopsis thaliana and Nicotiana benthamiana. J. Expt. Bot. 2018, 69, 5625–5635. [Google Scholar] [CrossRef]
  70. Park, K.Y.; Seo, S.Y.; Oh, B.-R.; Seo, J.-W.; Kim, Y.J. 2,3-Butanediol induces systemic acquired resistance in the plant immune response. J. Plant Biol. 2018, 61, 424–434. [Google Scholar] [CrossRef]
  71. Vlassi, A.; Nesler, A.; Perazzolli, M.; Lazazzara, V.; Büschl, C.; Parich, A.; Puopolo, G.; Schuhmacher, R. Volatile organic compounds from Lysobacter capsici AZ78 as potential candidates for biological control of soilborne plant pathogens. Front. Microbiol. 2020, 11, 1748. [Google Scholar] [CrossRef]
  72. Janamatti, A.T.; Kumar, A.; Kaur, C.; Gogoi, R.; Varghese, E.; Kumar, S. Fumigation by bacterial volatile 2,5-dimethylpyrazine enhances anthracnose resistance and shelf life of mango. Eur. J. Plant Pathol. 2022, 164, 209–227. [Google Scholar] [CrossRef]
  73. Siri-udom, S.; Suwannarach, N.; Lumyong, S. Applications of volatile compounds acquired from Muscodor heveae against white root rot disease in rubber trees (Hevea brasiliensis Müll. Arg.) and relevant allelopathy effects. Fun. Biol. 2017, 121, 573–581. [Google Scholar] [CrossRef]
  74. Liu, P.; Cheng, Y.; Yang, M.; Liu, Y.; Chen, K.; Long, C.-A.; Deng, X. Mechanisms of action for 2-phenylethanol isolated from Kloeckera apiculate in control of Penicillium molds of citrus fruits. BMC Microbiol. 2014, 14, 242. [Google Scholar] [CrossRef]
  75. Guimarães, A.; Venâncio, A. A Potential of fatty acids and their derivatives as antifungal agents: A Review. Toxins 2022, 14, 188. [Google Scholar] [CrossRef]
  76. Chadeganipour, M.; Haims, A. Antifungal activities of pelargonic and capric acid on Microsporum gypseum. Mycoses 2001, 44, 109–112. [Google Scholar] [CrossRef] [PubMed]
  77. Bergsson, G.; Arnfinnsson, J.; Steingrímsson, O.; Thormar, H. In vitro killing of Candida albicans by fatty acids and monoglycerides. Antimicrob. Agents Chemother. 2001, 45, 3209–3212. [Google Scholar] [CrossRef] [PubMed]
  78. Vicedo, B.; Flors, V.; Leyva, M.O.; Finiti, I.; Kravchuk, Z.; Real, M.D.; García-Agustín, P.; González-Bosch, C. Hexanoic acid-induced resistance against Botrytis cinerea in tomato plants. Mol. Plant Microbe Interact. 2009, 22, 1455–1465. [Google Scholar] [CrossRef] [PubMed]
  79. Raza, W.; Wei, Z.; Ling, N.; Huang, Q.; Shen, Q. Effect of organic fertilizers prepared from organic waste materials on the production of antibacterial volatile organic compounds by two biocontrol Bacillus amyloliquefaciens strains. J. Biotechnol. 2016, 277, 43–53. [Google Scholar] [CrossRef]
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Figure 1. Effect of Bacillus spp. VOCs on MoT in bi-partitioned Petri dish assay in vitro. (A) Photographs showing the effects of Bacillus VOCs on mycelial growth of MoT; (B) effects of Bacillus VOCs on mycelial growth of MoT in vitro; (C) effects of Bacillus VOCs on reduction in MoT sporulation compared to control in vitro. Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; control: without any bacterial treatment (Tukey test; n = 6; p ≤ 0.05). Black points in (B,C) represent data points for each replicate.
Figure 1. Effect of Bacillus spp. VOCs on MoT in bi-partitioned Petri dish assay in vitro. (A) Photographs showing the effects of Bacillus VOCs on mycelial growth of MoT; (B) effects of Bacillus VOCs on mycelial growth of MoT in vitro; (C) effects of Bacillus VOCs on reduction in MoT sporulation compared to control in vitro. Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; control: without any bacterial treatment (Tukey test; n = 6; p ≤ 0.05). Black points in (B,C) represent data points for each replicate.
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Figure 2. Effects of Bacillus spp. VOCs on MoT conidial germination in upside-down Petri dish assay in vitro. (A) Photographs showing the effects of Bacillus VOCs on MoT conidial germination and mycelial growth; (B) effects of bacterial VOCs on mycelial growth of MoT in vitro; (C) effects of bacterial VOCs on reduction in new MoT sporulation compared to control in vitro. Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; control: without any bacterial treatment (Tukey test; n = 6; p ≤ 0.05). Black points in (B,C) represent data points for each replicate.
Figure 2. Effects of Bacillus spp. VOCs on MoT conidial germination in upside-down Petri dish assay in vitro. (A) Photographs showing the effects of Bacillus VOCs on MoT conidial germination and mycelial growth; (B) effects of bacterial VOCs on mycelial growth of MoT in vitro; (C) effects of bacterial VOCs on reduction in new MoT sporulation compared to control in vitro. Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; control: without any bacterial treatment (Tukey test; n = 6; p ≤ 0.05). Black points in (B,C) represent data points for each replicate.
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Figure 3. Effects of Bacillus VOCs on lesion development and MoT sporulation in a detached wheat leaf assay (cv. BR-18). (A) VOCs from Bacillus spp. significantly reduced leaf blast lesion size in vivo. (B) Bacillus VOCs significantly reduced leaf lesion area (cm2) caused by MoT in vivo (Kruskal–Wallis test; n = 30; p ≤ 0.05); (C) reduction in MoT sporulation by VOCs from different Bacillus spp. in vivo (Tukey test; n = 30; p ≤ 0.05). Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; untreated control: only MoT inoculated. Black points in (B,C) represent data points for each replicate.
Figure 3. Effects of Bacillus VOCs on lesion development and MoT sporulation in a detached wheat leaf assay (cv. BR-18). (A) VOCs from Bacillus spp. significantly reduced leaf blast lesion size in vivo. (B) Bacillus VOCs significantly reduced leaf lesion area (cm2) caused by MoT in vivo (Kruskal–Wallis test; n = 30; p ≤ 0.05); (C) reduction in MoT sporulation by VOCs from different Bacillus spp. in vivo (Tukey test; n = 30; p ≤ 0.05). Data were recorded after 5 d of MoT incubation at 25 °C. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; consortium: a mixture of BTS-3, BTS-4, and BTLK6A; untreated control: only MoT inoculated. Black points in (B,C) represent data points for each replicate.
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Figure 4. Identification and quantification of VOCs from different Bacillus treatments by GC–MS analysis. (A) Venn diagram representing the number of VOCs produced from different Bacillus spp.; (B) number of different VOC classes produced from different Bacillus treatments; (C) heatmap based on Euclidean distance showing the VOCs produced from each Bacillus treatment. Each line in the color heatmap indicates a single compound; red to green color code indicates low to high relative concentrations (based on row Z-scores) of the compounds; blue color indicates compounds detected only in a single treatment; grey color indicates undetected volatile. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; const.: consortium (a mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) (n = 5; p ≤ 0.05). The VOCs were quantified from 40 mL LB inoculated with 100 µL (1 × 109 CFU/mL) bacteria and incubated for 4 d at 25 °C.
Figure 4. Identification and quantification of VOCs from different Bacillus treatments by GC–MS analysis. (A) Venn diagram representing the number of VOCs produced from different Bacillus spp.; (B) number of different VOC classes produced from different Bacillus treatments; (C) heatmap based on Euclidean distance showing the VOCs produced from each Bacillus treatment. Each line in the color heatmap indicates a single compound; red to green color code indicates low to high relative concentrations (based on row Z-scores) of the compounds; blue color indicates compounds detected only in a single treatment; grey color indicates undetected volatile. BTS-3: B. subtilis BTS-3; BTS-4: B. velezensis BTS-4; BTLK6A: B. velezensis BTLK6A; const.: consortium (a mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) (n = 5; p ≤ 0.05). The VOCs were quantified from 40 mL LB inoculated with 100 µL (1 × 109 CFU/mL) bacteria and incubated for 4 d at 25 °C.
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Figure 5. Fold change (FC) in VOCs produced by B. velezensis BTS-4 in relation to the Bacillus consortium (a mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) treatment through volcano plot analysis. The log2 fold change threshold was 2.0. The false discovery rate (FDR) was maintained with p threshold at 0.05. The VOCs were quantified from 40 mL LB inoculated with 100 µL (1 × 109 CFU/mL) bacteria and incubated for 4 d at 25 °C. Non-SIG: non-significant.
Figure 5. Fold change (FC) in VOCs produced by B. velezensis BTS-4 in relation to the Bacillus consortium (a mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) treatment through volcano plot analysis. The log2 fold change threshold was 2.0. The false discovery rate (FDR) was maintained with p threshold at 0.05. The VOCs were quantified from 40 mL LB inoculated with 100 µL (1 × 109 CFU/mL) bacteria and incubated for 4 d at 25 °C. Non-SIG: non-significant.
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Figure 6. Effect of selected pure antifungal VOCs against MoT mycelial growth and sporulation in vitro represented by heatmaps based on Euclidean distance: (A) effect of pure antifungal VOCs on MoT mycelial growth; (B) effect of pure antifungal VOCs on MoT sporulation. Each row in the color heatmap indicates a single compound. Red to green color code indicates low to high relative growth or sporulation of MoT (based on row Z-score). * indicates directly used single concentration (diluent) (n = 6, p ≤ 0.05).
Figure 6. Effect of selected pure antifungal VOCs against MoT mycelial growth and sporulation in vitro represented by heatmaps based on Euclidean distance: (A) effect of pure antifungal VOCs on MoT mycelial growth; (B) effect of pure antifungal VOCs on MoT sporulation. Each row in the color heatmap indicates a single compound. Red to green color code indicates low to high relative growth or sporulation of MoT (based on row Z-score). * indicates directly used single concentration (diluent) (n = 6, p ≤ 0.05).
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MDPI and ACS Style

Surovy, M.Z.; Rahman, S.; Rostás, M.; Islam, T.; von Tiedemann, A. Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast. Microorganisms 2023, 11, 1291. https://doi.org/10.3390/microorganisms11051291

AMA Style

Surovy MZ, Rahman S, Rostás M, Islam T, von Tiedemann A. Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast. Microorganisms. 2023; 11(5):1291. https://doi.org/10.3390/microorganisms11051291

Chicago/Turabian Style

Surovy, Musrat Zahan, Shahinoor Rahman, Michael Rostás, Tofazzal Islam, and Andreas von Tiedemann. 2023. "Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast" Microorganisms 11, no. 5: 1291. https://doi.org/10.3390/microorganisms11051291

APA Style

Surovy, M. Z., Rahman, S., Rostás, M., Islam, T., & von Tiedemann, A. (2023). Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast. Microorganisms, 11(5), 1291. https://doi.org/10.3390/microorganisms11051291

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