Animals

21 pages, 2409 KiB

Open AccessReview

by Kristyn R. Vitale

Animals 2022, 12(1), 126; https://doi.org/10.3390/ani12010126 - 5 Jan 2022

Cited by 27 | Viewed by 12857

Despite the diversity of social situations in which cats live, the degree to which free-ranging cats (FRCs) are social is still debated. The aim of this review is to explore the literature on the social behavior of FRCs. A search of two major [...] Read more.

Despite the diversity of social situations in which cats live, the degree to which free-ranging cats (FRCs) are social is still debated. The aim of this review is to explore the literature on the social behavior of FRCs. A search of two major databases revealed that observations of intraspecies and interspecies social interactions have been conducted. The intraspecific social dynamics of FRCs differ based on group of cats surveyed. Some groups display strong social bonds and preferential affiliations, while other groups are more loosely associated and display little to no social interaction. Factors impacting FRC conspecific interactions include cat body size, cat social rank, cat individuality, cat age, relationship to conspecific (kin/familiar), cat sex, level of human caretaking, presence of food, the health of the individual, or sexual status of conspecifics. Interspecies interactions also occur with humans and wildlife. The human’s sex and the weather conditions on the day of interaction have been shown to impact FRC social behavior. Interactions with wildlife were strongly linked to the timing of cat feeding events. These findings support the idea that FRCs are “social generalists” who display flexibility in their social behavior. The social lives of FRCs exist, are complex, and deserve further study. Full article

(This article belongs to the Special Issue Behavioural Ecology and Welfare of Free-Ranging Cats)

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12 pages, 414 KiB

Open AccessArticle

Changes in Fatty Acids Profile, Health Indices, and Physical Characteristics of Organic Eggs from Laying Hens at the Beginning of the First and Second Laying Cycles

by Lukáš Zita, Monika Okrouhlá, Ondřej Krunt, Adam Kraus, Luděk Stádník, Jaroslav Čítek and Roman Stupka

Animals 2022, 12(1), 125; https://doi.org/10.3390/ani12010125 - 5 Jan 2022

Cited by 9 | Viewed by 2729

Abstract

The present study compared the fatty acid profile and some physical parameters of eggs from hens reared according to the organic system at the beginning of the first and second laying cycle. A total of 1080 eggs were analysed at the beginning of [...] Read more.

The present study compared the fatty acid profile and some physical parameters of eggs from hens reared according to the organic system at the beginning of the first and second laying cycle. A total of 1080 eggs were analysed at the beginning of the first (from the 28th to 30th week of age) and the second (from the 78th to 80th week of age) laying cycle. It was found that the hen ages influenced the egg weight, egg surface area, yolk proportion, and eggshell colour. Albumen and eggshell proportion, albumen, yolk index, Haugh unit score, and eggshell strength were lower in eggs from older hens compared with those produced from younger layers. Monounsaturated fatty acids were found in higher amounts than saturated fatty acids and polyunsaturated fatty acids in egg yolks of eggs from layers only at the beginning of the second laying cycle. The PUFAn-6/n-3 ratio, saturation, atherogenic, and thrombogenic indices were significantly lower in the egg yolks from older hens compared to younger layers. These findings (regarding the eggs from the older ones) prove that it is practical to utilize them in the organic farming system during a period of two years. Full article

(This article belongs to the Special Issue Nutrition and Management of Egg-Laying Poultry)

10 pages, 32522 KiB

Open AccessReview

Canine and Feline Testicular Preservation

by Lúcia Daniel Machado da Silva

Animals 2022, 12(1), 124; https://doi.org/10.3390/ani12010124 - 5 Jan 2022

Cited by 3 | Viewed by 2936

Abstract

The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of [...] Read more.

The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats. Full article

(This article belongs to the Special Issue Biotechnology and Reproduction in Companion Animals)

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10 pages, 267 KiB

Open AccessArticle

Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves

by Dong Wang, Zhendong You, Yuanyi Du, Duo Zheng, Haotian Jia and Yun Liu

Animals 2022, 12(1), 123; https://doi.org/10.3390/ani12010123 - 5 Jan 2022

Cited by 7 | Viewed by 2996

Abstract

This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four [...] Read more.

This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four treatment groups: (1) control (basal diet); (2) 1-gram NaH (basal diet extra orally supplemented with 1 g of NaH dissolved in 100 mL of milk or milk replacer daily); (3) 3-gram NaH (basal diet extra orally supplemented with 3 g of NaH dissolved in 100 mL of milk or milk replacer daily); and (4) 5-gram NaH (basal diet extra orally supplemented with 5 g of NaH dissolved in 100 mL of milk or milk replacer daily). NaH was mixed with milk (d 2–20) or milk replacer (d 21–53). Calves in the 5-gram NaH group had a higher ADG during d 1 to 21 and d 21 to 53 than the other groups did (p < 0.05). Fecal scores and diarrheal incidence were significantly lower in the 3-gram and 5-gram NaH groups than the 1-gram NaH and control groups during d 1 to 20 (p < 0.05). The serum IgA, IgG and IL-4 concentrations, and T-SOD and T-AOC activities were higher, and the serum IL-6, TNF-α, D-lactic acid, and MDA concentrations were lower in the 5-gram NaH group than the control group (p < 0.05). Furthermore, NaH supplementation increased the abundances of Bifidobacterium and Lactobacillus but decreased the abundance of Escherichia coli in feces (p < 0.05). These encouraging findings indicated that supplementation with 5 g of NaH effectively improved the immune status, antioxidant capacity, and intestinal beneficial bacteria, and further improved the growth performance and reduced the diarrhea incidence of the pre-weaned dairy calves. Full article

(This article belongs to the Section Animal Nutrition)

15 pages, 1306 KiB

Open AccessArticle

Owner-Perception of the Effects of Two Long-Lasting Dog-Appeasing Pheromone Analog Devices on Situational Stress in Dogs

by Céline S. Nicolas, Gemma Espuña, Aurélie Girardin, Jaume Fatjó, Jonathan Bowen and Patricia Monginoux

Animals 2022, 12(1), 122; https://doi.org/10.3390/ani12010122 - 5 Jan 2022

Viewed by 3636

Abstract

Devices that release a synthetic analog of the canine-appeasing pheromone can help to relax dogs during stressful situations, but they usually last for only one month. Two new devices with this analog were tested by owners of dogs showing signs of stress in [...] Read more.

Devices that release a synthetic analog of the canine-appeasing pheromone can help to relax dogs during stressful situations, but they usually last for only one month. Two new devices with this analog were tested by owners of dogs showing signs of stress in a range of everyday situations: Zenidog™ collar, lasting three months, and Zenidog™ diffusing gel, lasting two months (Virbac, Carros, France). They were compared against reference products that last for one month. In the three-month study with collars, one group received Zenidog™ collar, one received the reference collar, and one group of dogs wore an antiparasitic collar alongside a Zenidog™ collar. In the two-month study with diffusers, groups received either the unpowered Zenidog™ gel diffuser or the reference electric diffuser. Owners regularly completed a questionnaire that assessed seventeen general behaviors and sources of fear and eleven specific signs of stress. Global scores for these two main scales were calculated, and the evolution of scores was compared between groups. Non-parametric tests with a Bonferroni correction were used for statistical analysis. An improvement of all global scores was observed in all groups (p < 0.001), including in puppies, and there was no difference between groups. Zenidog™ devices were as effective as the reference devices and lasted longer. Full article

(This article belongs to the Section Companion Animals)

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18 pages, 3723 KiB

Open AccessArticle

Dietary Supplementation with Goji Berries (Lycium barbarum) Modulates the Microbiota of Digestive Tract and Caecal Metabolites in Rabbits

by Paola Cremonesi, Giulio Curone, Filippo Biscarini, Elisa Cotozzolo, Laura Menchetti, Federica Riva, Maria Laura Marongiu, Bianca Castiglioni, Olimpia Barbato, Albana Munga, Marta Castrica, Daniele Vigo, Majlind Sulce, Alda Quattrone, Stella Agradi and Gabriele Brecchia

Animals 2022, 12(1), 121; https://doi.org/10.3390/ani12010121 - 5 Jan 2022

Cited by 18 | Viewed by 3596

Abstract

Goji berries show health benefits, although the possible mechanisms of action, including compositional changes in the gut microbiome, are still not fully understood. The aim of this study was to evaluate the effect of Goji berry supplementation on microbiota composition and metabolites in [...] Read more.

Goji berries show health benefits, although the possible mechanisms of action, including compositional changes in the gut microbiome, are still not fully understood. The aim of this study was to evaluate the effect of Goji berry supplementation on microbiota composition and metabolites in the digestive tracts of rabbits. Twenty-eight New Zealand White rabbits were fed with a commercial feed (control group, C; n = 14) or the same diet supplemented with 3% of Goji berries (Goji group, G; n = 14), from weaning (35 days old) until slaughter (90 days old). At slaughter, samples from the content of the gastrointestinal tracts were collected and analyzed by Next Generation 16S rRNA Gene Sequencing to evaluate the microbial composition. Ammonia and lactic acid were also quantified in caecum. Results showed differences in microbiota composition between the groups for two phyla (Cyanobacteria and Euryarchaeota), two classes (Methanobacteria and Bacilli), five orders, fourteen families, and forty-five genera. Ruminococcaceae (p < 0.05) and Lachnospiraceae (p < 0.01) were more abundant in G than in C group. Lactobacillaceae also showed differences between the two groups, with Lactobacillus as the predominant genus (p = 0.002). Finally, Goji berry supplementation stimulated lactic acid fermentation (p < 0.05). Thus, Goji berry supplementation could modulate gastrointestinal microbiota composition and caecal fermentation. Full article

(This article belongs to the Special Issue Natural Feed Additives and Novel Approaches for Healthy Rabbit Breeding)

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11 pages, 1376 KiB

Open AccessArticle

Length at Maturity, Sex Ratio, and Proportions of Maturity of the Giant Electric Ray, Narcine entemedor, in Its Septentrional Distribution

by Xchel Aurora Pérez-Palafox, Enrique Morales-Bojórquez, Hugo Aguirre-Villaseñor and Víctor Hugo Cruz-Escalona

Animals 2022, 12(1), 120; https://doi.org/10.3390/ani12010120 - 5 Jan 2022

Cited by 4 | Viewed by 3835

Abstract

The size at which a certain fraction of a fish population reaches sexual maturity is an important parameter of life history. The estimation of this parameter based on logistic or sigmoid models could provide different ogives and values of length at maturity, which [...] Read more.

The size at which a certain fraction of a fish population reaches sexual maturity is an important parameter of life history. The estimation of this parameter based on logistic or sigmoid models could provide different ogives and values of length at maturity, which must be analyzed and considered as a basic feature of biological reproduction for the species. A total of 305 individuals of Narcine entemedor (N. entemedor) were obtained from artisanal fisheries in the Bahía de La Paz, Mexico. For the organisms sampled, sexes were determined and total length (TL) in cm was measured from October 2013 to December 2015. The results indicated that the females were larger, ranging from 48.5 cm to 84 cm TL, while males varied from 41.5 cm to 58.5 cm TL. The sex ratio was dominated by males ranging from 45–55 cm TL, while females were more abundant from 60 to 85 cm TL. Mature females were present all year long, exhibiting a continuous annual reproductive cycle. The length at maturity data were described by the Gompertz model with value of 55.87 cm TL. The comparison between models, and the model selection between them, showed that the Gompertz model had maximum likelihood and smaller Akaike information criterion, indicating that this model was a better fit to the maturity proportion data of N. entemedor. Full article

(This article belongs to the Special Issue Sharks and Skates: Ecology, Distribution and Conservation)

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12 pages, 1671 KiB

Open AccessArticle

ACSL4 Directs Intramuscular Adipogenesis and Fatty Acid Composition in Pigs

by Hongyan Ren, Haoyuan Zhang, Zaidong Hua, Zhe Zhu, Jiashu Tao, Hongwei Xiao, Liping Zhang, Yanzhen Bi and Heng Wang

Animals 2022, 12(1), 119; https://doi.org/10.3390/ani12010119 - 4 Jan 2022

Cited by 9 | Viewed by 2931

Abstract

The intramuscular fat is a major quality trait of meat, affecting sensory attributes such as flavor and texture. Several previous GWAS studies identified Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4) gene as the candidate gene to regulate intramuscular fat content in different [...] Read more.

The intramuscular fat is a major quality trait of meat, affecting sensory attributes such as flavor and texture. Several previous GWAS studies identified Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4) gene as the candidate gene to regulate intramuscular fat content in different pig populations, but the underlying molecular function of ACSL4 in adipogenesis within pig skeletal muscle is not fully investigated. In this study, we isolated porcine endogenous intramuscular adipocyte progenitors and performed ACSL4 loss- and gain-of-function experiments during adipogenic differentiation. Our data showed that ACSL4 is a positive regulator of adipogenesis in intramuscular fat cells isolated from pigs. More interestingly, the enhanced expression of ACSL4 in pig intramuscular adipocytes could increase the cellular content of monounsaturated and polyunsaturated fatty acids, such as gamma-L eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA). The above results not only confirmed the function of ACSL4 in pig intramuscular adipogenesis and meat quality attributes, but also provided new clues for the improvement of the nutritional value of pork for human health. Full article

(This article belongs to the Collection Genomics, Transcriptomics, and Computational Biology for Biodiversity Studies and Quality-Related Traits Selection in Livestock)

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14 pages, 6507 KiB

Open AccessArticle

Effect of Oregano Oil and Cobalt Lactate on Sheep In Vitro Digestibility, Fermentation Characteristics and Rumen Microbial Community

by Zhengwen Wang, Xiongxiong Li, Lingyun Zhang, Jianping Wu, Shengguo Zhao and Ting Jiao

Animals 2022, 12(1), 118; https://doi.org/10.3390/ani12010118 - 4 Jan 2022

Cited by 27 | Viewed by 2884

Abstract

The objective of this experiment was to evaluate the effect of different EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) levels on in vitro rumen fermentation and microbial changes. Six EOC levels (treatments: 0 mg·L⁻¹, CON; 50 [...] Read more.

The objective of this experiment was to evaluate the effect of different EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) levels on in vitro rumen fermentation and microbial changes. Six EOC levels (treatments: 0 mg·L⁻¹, CON; 50 mg·L⁻¹, EOC1; 100 mg·L⁻¹, EOC2; 400 mg·L⁻¹, EOC3; 800 mg·L⁻¹, EOC4 and 1500 mg·L⁻¹, EOC5) were selected to be used to in vitro incubation. The in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro acid detergent fiber digestibility (IVNDFD), pH, ammonia-nitrogen (NH₃-N) concentration, total volatile fatty acid (TVFA) concentration and microbial protein (MCP) concentration were measured after 48 h incubation, after which the groups with significant nutrient digestibility and fermentation parameters were subjected to 16S rRNA sequencing. The results showed that the total gas production (GP) of the EOC5 group was higher than that of the other groups after 12 h of in vitro incubation. TVFA, NH₃-N and MCP concentrations were also shown to be higher in group EOC5 than those in other groups (p < 0.05), while NH₃-N and MCP concentrations in the EOC2 group were lower than those in other groups significantly (p < 0.05). The molar ratio of acetic acid decreased while the molar ratio of propionic acid increased after the addition of EOC. 16S rRNA sequencing revealed that the rumen microbiota was altered in response to adding EOC, especially for the EOC5 treatment, with firmicutes shown to be the most abundant (43.1%). The relative abundance of Rikenellaceae_RC9_gut_group was significantly lower, while the relative abundance of uncultured_bacterium_f_Muribaculaceae and Succiniclasticum was significantly higher in the EOC5 group than those in other groups (p < 0.05). Comprehensive analysis showed that EOC (1500 mg·L⁻¹) could significantly increase gas production, alter sheep rumen fermentation parameters and microbiota composition. Full article

(This article belongs to the Section Animal Nutrition)

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10 pages, 522 KiB

Open AccessArticle

Antibiotic Resistance Patterns in Cervical Microbes of Gilts and Sows

by Cecilia Kellerman, Pongpreecha Malaluang, Ingrid Hansson, Lena Eliasson Selling and Jane M. Morrell

Animals 2022, 12(1), 117; https://doi.org/10.3390/ani12010117 - 4 Jan 2022

Cited by 2 | Viewed by 2151

Abstract

Extenders for boar semen contain antibiotics, which may induce antimicrobial resistance (AMR) in inseminated females. The objective was to investigate AMR of bacteria isolated from the cervix of sows and gilts in standing heat, representing females previously exposed to antibiotics in the semen [...] Read more.

Extenders for boar semen contain antibiotics, which may induce antimicrobial resistance (AMR) in inseminated females. The objective was to investigate AMR of bacteria isolated from the cervix of sows and gilts in standing heat, representing females previously exposed to antibiotics in the semen extender and non-exposed females, respectively. Cervical swabs were taken from 30 multiparous sows and 30 gilts prior to their first insemination. After culturing on agar plates, bacterial isolates were identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry and antimicrobial minimum inhibitory concentrations (MIC) were determined. Differences in antibiotic resistance between sows and gilts were analyzed by Chi-squared or Fisher’s exact test. Bacteria isolated were mostly Staphylococcus spp., Streptococcus spp. and Corynebacterium spp. Higher MICs were observed for isolates from sows than from gilts. Most (>80%) Corynebacterium spp. were resistant to clindamycin; small numbers (<20%) were resistant to gentamicin, penicillin, vancomycin, ciprofloxacin and rifampicin, with no differences between gilts and sows. Corynebacterium from gilts were more often resistant to tetracycline than those from sows (25% vs. 4.17%; p = 0.04). In conclusion, bacteria from the porcine cervix showed low resistance to most antibiotics except for clindamycin, but antibacterial resistance may increase with increasing parity. Full article

(This article belongs to the Special Issue Advances in Pig Reproduction)

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14 pages, 282 KiB

Open AccessArticle

Promoting Judicious Antimicrobial Use in Beef Production: The Role of Quarantine

by Matteo Santinello, Alessia Diana, Massimo De Marchi, Federico Scali, Luigi Bertocchi, Valentina Lorenzi, Giovanni Loris Alborali and Mauro Penasa

Animals 2022, 12(1), 116; https://doi.org/10.3390/ani12010116 - 4 Jan 2022

Cited by 8 | Viewed by 2559

Abstract

Judicious antimicrobial stewardship in livestock industry is needed to reduce the use of antimicrobials (AMU) and the associated risk of antimicrobial resistance. Biosecurity measures are acknowledged for their role against the spread of diseases and the importance in reducing AMU in different species. [...] Read more.

Judicious antimicrobial stewardship in livestock industry is needed to reduce the use of antimicrobials (AMU) and the associated risk of antimicrobial resistance. Biosecurity measures are acknowledged for their role against the spread of diseases and the importance in reducing AMU in different species. However, their effectiveness in beef production has been scarcely considered. The aim of this study was to investigate the effect of the quarantine strategy on AMU in beef cattle. A total of 1206 Charolaise animals in five farms were included in the trial. Roughly half of the animals followed the standard procedure of the fattening cycle (no-quarantine; NO-QUA group) and half followed a 30-day period of quarantine (QUA group) since their arrival. Performance and antimicrobial data were recorded and a treatment incidence 100 (TI100it) per animal was calculated. Penicillins was the most used class of antimicrobials. Differences between groups were significant for males only, with NO-QUA group having greater TI100it (3.76 vs. 3.24; p < 0.05) and lower body weight at slaughter (713.4 vs. 723.7 kg; p < 0.05) than QUA group. Results suggest that quarantine strategy can reduce AMU in males without compromising their performance, whereas further investigation is needed for females. Full article

(This article belongs to the Section Animal System and Management)

12 pages, 670 KiB

Open AccessArticle

African Swine Fever Outbreaks in Lithuanian Domestic Pigs in 2019

by Alvydas Malakauskas, Katja Schulz, Indrė Kukanauskaitė, Marius Masiulis, Franz Josef Conraths and Carola Sauter-Louis

Animals 2022, 12(1), 115; https://doi.org/10.3390/ani12010115 - 4 Jan 2022

Cited by 9 | Viewed by 3041

Abstract

While numerous risk factors of African swine fever (ASF) transmission to domestic pigs have been described, ASF virus introduction has often not been traced back to one single defined cause. The large number of ASF outbreaks that occurred in domestic pigs in Lithuania [...] Read more.

While numerous risk factors of African swine fever (ASF) transmission to domestic pigs have been described, ASF virus introduction has often not been traced back to one single defined cause. The large number of ASF outbreaks that occurred in domestic pigs in Lithuania from 2014 through to 2018 raised the question regarding whether outbreak-specific risk factors and transmission routes could be identified. Therefore, a prospective matched case-control study was designed. Data from 18 outbreaks that occurred in Lithuanian in 2019 and 36 control farms were analyzed. Conditional multivariable logistic regression showed that two or more visits by veterinary inspection of a farm had a significant preventive effect on the occurrence of ASF on a farm (Odds ratio (OR) 14.21, confidence interval (CI) 1.09–185.60 for farms not inspected vs. farms inspected twice or more a year), while certain practices (e.g., mushroom picking, sharing equipment, etc.), which might facilitate the indirect introduction of ASF from fields and forests into piggeries, significantly increased the odds of an outbreak (OR 5.18, CI 1.10–24.44). The results of the study highlight the importance of veterinary inspections for increasing the biosecurity level on pig farms and the awareness of ASF. The knowledge on potential protective and risk factors may help to improve the prevention and control of ASF outbreaks in domestic pig farms in Lithuania and other affected countries. Full article

(This article belongs to the Section Pigs)

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14 pages, 5112 KiB

Open AccessArticle

MiR-22-3p Inhibits Proliferation and Promotes Differentiation of Skeletal Muscle Cells by Targeting IGFBP3 in Hu Sheep

by Shan Wang, Xiukai Cao, Ling Ge, Yifei Gu, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile and Wei Sun

Animals 2022, 12(1), 114; https://doi.org/10.3390/ani12010114 - 4 Jan 2022

Cited by 13 | Viewed by 2557

Abstract

The growth and development of skeletal muscle require a series of regulatory factors. MiRNA is a non-coding RNA with a length of about 22 nt, which can inhibit the expression of mRNA and plays an important role in the growth and development of [...] Read more.

The growth and development of skeletal muscle require a series of regulatory factors. MiRNA is a non-coding RNA with a length of about 22 nt, which can inhibit the expression of mRNA and plays an important role in the growth and development of muscle cells. The role of miR-22-3p in C2C12 cells and porcine skeletal muscle has been reported, but it has not been verified in Hu sheep skeletal muscle. Through qPCR, CCK-8, EdU and cell cycle studies, we found that overexpression of miR-22-3p inhibited proliferation of skeletal muscle cells (p < 0.01). The results of qPCR and immunofluorescence showed that overexpression of miR-22-3p promoted differentiation of skeletal muscle cells (p < 0.01), while the results of inhibiting the expression of miR-22-3p were the opposite. These results suggested that miR-22-3p functions in growth and development of sheep skeletal muscle cells. Bioinformatic analysis with mirDIP, miRTargets, and RNAhybrid software suggested IGFBP3 was the target of miR-22-3p, which was confirmed by dual-luciferase reporter system assay. IGFBP3 is highly expressed in sheep skeletal muscle cells. Overexpression of IGFBP3 was found to promote proliferation of skeletal muscle cells indicated by qPCR, CCK-8, EdU, and cell cycle studies (p < 0.01). The results of qPCR and immunofluorescence experiments proved that overexpression of IGFBP3 inhibited differentiation of skeletal muscle cells (p < 0.01), while the results of interfering IGFBP3 with siRNA were the opposite. These results indicate that miR-22-3p is involved in proliferation and differentiation of skeletal muscle cells by targeting IGFBP3. Full article

(This article belongs to the Special Issue Genomics and Phenomics for Improving the Sustainability of Livestock Production)

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13 pages, 2039 KiB

Open AccessFeature PaperArticle

Implementation of a Pig Toilet in a Nursery Pen with a Straw-Littered Lying Area

by Michelle Tillmanns, Kees Scheepens, Marieke Stolte, Swetlana Herbrandt, Nicole Kemper and Michaela Fels

Animals 2022, 12(1), 113; https://doi.org/10.3390/ani12010113 - 4 Jan 2022

Cited by 10 | Viewed by 3735

Abstract

In this study, a pig toilet was installed on an organic pig farm, which enabled pigs to use a lying area littered with straw and keep it clean. The pig toilet was separated into a defaecation area and a urination area and nursery [...] Read more.

In this study, a pig toilet was installed on an organic pig farm, which enabled pigs to use a lying area littered with straw and keep it clean. The pig toilet was separated into a defaecation area and a urination area and nursery pigs were trained to use the urination area by means of a rewarding system. A total of 24 piglets were weaned at 6–7 weeks of age and housed in the experimental system for four-week periods. Per trial, a group of four pigs was formed, and videos were recorded on two days per week (08:00 to 18:00). Direct observation was carried out in the first and last week of each trial. In total, 1500 eliminations were video-analysed. An individual pig had an average of 7.1 ± 1.4 defaecations and 4.8 ± 0.8 urinations per day. In total, 96.4% of all urinations and 97.4% of all defaecations were performed in the pig toilet. However, most urinations took place in the defaecation area as well (90.4%). Even if the training to spatially separate defecation and urination behaviour was not successful, we showed that a pig toilet offers the possibility to create littered lying areas, possibly increasing animal welfare. Full article

(This article belongs to the Collection Behaviour of Pigs in Relation to Housing Environment)

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15 pages, 314 KiB

Open AccessArticle

Effects of Dietary Incorporation of Grape Stalks Untreated and Fungi-Treated in Growing Rabbits: A Preliminary Study

by Valéria Costa-Silva, Victor Pinheiro, Anabela Alves, José António Silva, Guilhermina Marques, Jose Lorenzo, Miguel Rodrigues and Luís Ferreira

Animals 2022, 12(1), 112; https://doi.org/10.3390/ani12010112 - 4 Jan 2022

Cited by 4 | Viewed by 2407

Abstract

This study aimed to evaluate the effect of the incorporation of untreated grape stalks (U_GS) and fungi-treated grape stalks (Lentinula edodes, T_GS) in rabbits’ diets. The control group was fed with a control diet without grape stalks [...] Read more.

This study aimed to evaluate the effect of the incorporation of untreated grape stalks (U_GS) and fungi-treated grape stalks (Lentinula edodes, T_GS) in rabbits’ diets. The control group was fed with a control diet without grape stalks (C), two experimental groups were fed on diets with 5% and 10% incorporation of U_GS (5U_GS and 10U_GS), and two with 5% and 10% incorporation of T_GS (5T_GS and 10T_GS). Rabbits fed with T_GS diets showed higher daily weight gain (p = 0.034), feed conversion rate (p = 0.002), carcass weight (p = 0.038), and reference carcass weight (p = 0.03) when compared to the control diet. Moreover, animals fed with T_GS diets showed an increase in the caecum (p = 0.015) and small intestine (p = 0.021) lengths and in the total volatile fatty acid content (p = 0.005) compared to animals fed U_GS diets. Blood triglyceride levels were lower in animals fed with T_GS diets compared to U_GS (p = 0.005) and C (p ≤ 0.001) diets (12% and 19% lower, respectively), and a trend to lower cholesterol levels was observed (p = 0.071). Meat from rabbits fed with T_GS diets had higher levels of linoleic acid, γ-linolenic, ∑ω-6, ∑PUFA, and ∑PUFA/∑SFA ratio compared to rabbits fed with the C diet. Results indicated that grape stalks (U_GS and T_GS) could be effectively used as an alternative raw material in rabbits’ diets without compromising animal performance. Full article

(This article belongs to the Topic Development of Agricultural and Food By-Product Resources and Animal Production to Achieve Food Sustainability)

23 pages, 2738 KiB

Open AccessArticle

Impact of Dietary Sodium Butyrate and Salinomycin on Performance and Intestinal Microbiota in a Broiler Gut Leakage Model

by Mohammad Naghizadeh, Laura Klaver, Anna A. Schönherz, Sundas Rani, Tina Sørensen Dalgaard and Ricarda Margarete Engberg

Animals 2022, 12(1), 111; https://doi.org/10.3390/ani12010111 - 4 Jan 2022

Cited by 10 | Viewed by 3186

Abstract

Unfavorable alterations of the commensal gut microbiota and dysbacteriosis is a major health problem in the poultry industry. Understanding how dietary intervention alters the microbial ecology of broiler chickens is important for prevention strategies. A trial was conducted with 672 Ross 308 day-old [...] Read more.

Unfavorable alterations of the commensal gut microbiota and dysbacteriosis is a major health problem in the poultry industry. Understanding how dietary intervention alters the microbial ecology of broiler chickens is important for prevention strategies. A trial was conducted with 672 Ross 308 day-old male broilers fed a basic diet (no additives, control) or the basic diet supplemented with 500 mg/kg encapsulated butyrate or 68 mg/kg salinomycin. Enteric challenge was induced by inclusion of 50 g/kg rye in a grower diet and oral gavage of a 10 times overdose of a vaccine against coccidiosis. Compared to control and butyrate-supplemented birds, salinomycin supplementation alleviated growth depression. Compared to butyrate and non-supplemented control, salinomycin increased potentially beneficial Ruminococcaceae and reduced potentially pathogenic Enterobacteriaceae and counts of Lactobacillus salivarius and Clostridium perfringens. Further, salinomycin supplementation was accompanied by a pH decrease and succinic acid increase in ceca, while coated butyrate (0.5 g/kg) showed no or limited effects. Salinomycin alleviated growth depression and maintained intestinal homeostasis in the challenged broilers, while butyrate in the tested concentration showed limited effects. Thus, further investigations are required to identify optimal dietary inclusion rates for butyrate used as alternative to ionophore coccidiostats in broiler production. Full article

(This article belongs to the Special Issue Gut Health in Poultry)

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11 pages, 956 KiB

Open AccessArticle

Physicochemical Characteristics of Yogurt from Sheep Fed with Moringa oleifera Leaf Extracts

by Miriam M. Mendoza-Taco, Aldenamar Cruz-Hernández, Angélica A. Ochoa-Flores, Josafat A. Hernández-Becerra, Armando Gómez-Vázquez, Victor M. Moo-Huchin, Ángel Piñeiro-Vázquez, Alfonso J. Chay-Canul and Einar Vargas-Bello-Pérez

Animals 2022, 12(1), 110; https://doi.org/10.3390/ani12010110 - 4 Jan 2022

Cited by 10 | Viewed by 2915

Abstract

This study determined the effect of feeding Moringa oleifera (MO) leaf extracts to lactating ewes on the physicochemical composition of their milk and yogurt during storage (4 °C for 14 days) and the sensory acceptance of the yogurt. Over 45 days, 24 multiparous [...] Read more.

This study determined the effect of feeding Moringa oleifera (MO) leaf extracts to lactating ewes on the physicochemical composition of their milk and yogurt during storage (4 °C for 14 days) and the sensory acceptance of the yogurt. Over 45 days, 24 multiparous lactating Pelibuey and Katahdin ewes (two days in lactation) were randomly assigned to four groups: MO-0, basal diet (BD) + 0 mL MO; MO-20, BD + 20 mL MO; MO-40, BD + 40 mL MO; and MO-60, BD + 60 mL MO. In the milk, an increase of 6% in protein, 26% in leucine, 14% in ash, and 1% in the pH (6.71) was observed with MO-60. The density values decreased by 0.3% at a higher dose of MO compared to MO-0, while the nonfat solids (NFS) in the milk were similar between the treatments. In the yogurt, an increase of 5% in protein, 113% in leucine (MO-20), 9% in NFS, and a reduction of 2% in moisture with MO-60 was observed. The acidity reflected an inverse relationship to the pH, as did the moisture and NFS with MO-60. In conclusion, dietary supplementation with MO in lactating ewes did not have negative effects on the chemical composition of their yogurt during storage (14 days). Overall, feeding sheep with 20 mL of MO positively influenced the physicochemical composition of their milk and yogurt during storage. Full article

(This article belongs to the Special Issue Interfaces between Ruminant Nutrition, Ruminant Physiology and Quality of Products)

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16 pages, 660 KiB

Open AccessEditor’s ChoiceArticle

Effects of Dietary Cereal and Protein Source on Fiber Digestibility, Composition, and Metabolic Activity of the Intestinal Microbiota in Weaner Piglets

by Carola Ellner, Anna G. Wessels and Jürgen Zentek

Animals 2022, 12(1), 109; https://doi.org/10.3390/ani12010109 - 4 Jan 2022

Cited by 11 | Viewed by 3089

Abstract

This study aimed to investigate the effect of fiber-rich rye and rapeseed meal (RSM) compared to wheat and soybean meal (SBM) on fiber digestibility and the composition and metabolic activity of intestinal microbiota. At weaning, 88 piglets were allocated to four feeding groups: [...] Read more.

This study aimed to investigate the effect of fiber-rich rye and rapeseed meal (RSM) compared to wheat and soybean meal (SBM) on fiber digestibility and the composition and metabolic activity of intestinal microbiota. At weaning, 88 piglets were allocated to four feeding groups: wheat/SBM, wheat/RSM, rye/SBM, and rye/RSM. Dietary inclusion level was 48% for rye and wheat, 25% for SBM, and 30% for RSM. Piglets were euthanized after 33 days for collection of digesta and feces. Samples were analyzed for dry matter and non-starch-polysaccharide (NSP) digestibility, bacterial metabolites, and relative abundance of microbiota. Rye-based diets had higher concentrations of soluble NSP than wheat-based diets. RSM-diets were higher in insoluble NSP compared to SBM. Rye-fed piglets showed a higher colonic and fecal digestibility of NSP (p < 0.001, p = 0.001, respectively). RSM-fed piglets showed a lower colonic and fecal digestibility of NSP than SBM-fed piglets (p < 0.001). Rye increased jejunal and colonic concentration of short-chain fatty acids (SCFA) compared to wheat (p < 0.001, p = 0.016, respectively). RSM-fed pigs showed a lower jejunal concentration of SCFA (p = 0.001) than SBM-fed pigs. Relative abundance of Firmicutes was higher (p = 0.039) and of Proteobacteria lower (p = 0.002) in rye-fed pigs compared to wheat. RSM reduced Firmicutes and increased Actinobacteria (jejunum, colon, feces: p < 0.050), jejunal Proteobacteria (p = 0.019) and colonic Bacteroidetes (p = 0.014). Despite a similar composition of the colonic microbiota, the higher amount and solubility of NSP from rye resulted in an increased fermentative activity compared to wheat. The high amount of insoluble dietary fiber in RSM-based diets reduced bacterial metabolic activity and caused a shift toward insoluble fiber degrading bacteria. Further research should focus on host–microbiota interaction to improve feeding concepts with a targeted use of dietary fiber. Full article

(This article belongs to the Special Issue The Impact of Nutrition on Swine Gastrointestinal Tract Health and Microbiota)

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10 pages, 1557 KiB

Open AccessArticle

Using Live and Video Stimuli to Localize Face and Object Processing Regions of the Canine Brain

by Kirsten D. Gillette, Erin M. Phillips, Daniel D. Dilks and Gregory S. Berns

Animals 2022, 12(1), 108; https://doi.org/10.3390/ani12010108 - 4 Jan 2022

Cited by 3 | Viewed by 3203

Abstract

Previous research to localize face areas in dogs’ brains has generally relied on static images or videos. However, most dogs do not naturally engage with two-dimensional images, raising the question of whether dogs perceive such images as representations of real faces and objects. [...] Read more.

Previous research to localize face areas in dogs’ brains has generally relied on static images or videos. However, most dogs do not naturally engage with two-dimensional images, raising the question of whether dogs perceive such images as representations of real faces and objects. To measure the equivalency of live and two-dimensional stimuli in the dog’s brain, during functional magnetic resonance imaging (fMRI) we presented dogs and humans with live-action stimuli (actors and objects) as well as videos of the same actors and objects. The dogs (n = 7) and humans (n = 5) were presented with 20 s blocks of faces and objects in random order. In dogs, we found significant areas of increased activation in the putative dog face area, and in humans, we found significant areas of increased activation in the fusiform face area to both live and video stimuli. In both dogs and humans, we found areas of significant activation in the posterior superior temporal sulcus (ectosylvian fissure in dogs) and the lateral occipital complex (entolateral gyrus in dogs) to both live and video stimuli. Of these regions of interest, only the area along the ectosylvian fissure in dogs showed significantly more activation to live faces than to video faces, whereas, in humans, both the fusiform face area and posterior superior temporal sulcus responded significantly more to live conditions than video conditions. However, using the video conditions alone, we were able to localize all regions of interest in both dogs and humans. Therefore, videos can be used to localize these regions of interest, though live conditions may be more salient. Full article

(This article belongs to the Special Issue Novel Approaches to Comparative Study of Human and Animal Emotions)

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13 pages, 854 KiB

Open AccessArticle

Therapy of Pyothorax in Cats via Small-Bore Thoracostomy Tube in Terms of Efficacy, Complications and Outcomes

by Evelyn Heier, Gabriel Wurtinger, Esther Hassdenteufel and Matthias Schneider

Animals 2022, 12(1), 107; https://doi.org/10.3390/ani12010107 - 3 Jan 2022

Cited by 3 | Viewed by 4674

Abstract

First-line therapy for cats with pyothorax consists of intravenous antibiotics, drainage of the septic pleural effusion and closed-chest lavage. Large-bore thoracostomy tubes are traditionally used for drainage, but case series indicate a comparable efficacy using small-bore tubes. In this retrospective study, we describe [...] Read more.

First-line therapy for cats with pyothorax consists of intravenous antibiotics, drainage of the septic pleural effusion and closed-chest lavage. Large-bore thoracostomy tubes are traditionally used for drainage, but case series indicate a comparable efficacy using small-bore tubes. In this retrospective study, we describe a new technique of sheath-guided small-bore (6 F) thoracostomy tubes in cats with pyothorax and evaluate their efficacy and complications. Additionally, we compare outcomes between two treatment groups. Placement and use of the small-bore thoracostomy tubes described here has a low complication rate of 4% (3/67 tubes), and 53% (24/45) of the cats could be treated with thoracostomy tubes and closed-chest lavage according to the protocol. The success rate is reduced by 18% (8/45) due to deaths caused mainly by sepsis, 16% (7/45) due to structural diseases requiring surgery and a further 14% (6/43) due to lavage failures that could only be cured after additive therapy (thoracotomy or fibrinolysis). The long-term prognosis was very good, with a survival rate one year after discharge of 94% (30/32). We detected no effect on survival by early placement of bilateral thoracostomy tubes or closed-chest lavage with a heparinised solution. In conclusion, therapy of pyothorax with small-bore thoracostomy tubes is as successful as therapy with large- or medium-bore tubes. Full article

(This article belongs to the Section Companion Animals)

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21 pages, 2577 KiB

Open AccessReview

Transient Receptor Potential (TRP) and Thermoregulation in Animals: Structural Biology and Neurophysiological Aspects

by Karina Lezama-García, Daniel Mota-Rojas, Alfredo M. F. Pereira, Julio Martínez-Burnes, Marcelo Ghezzi, Adriana Domínguez, Jocelyn Gómez, Ana de Mira Geraldo, Pamela Lendez, Ismael Hernández-Ávalos, Isabel Falcón, Adriana Olmos-Hernández and Dehua Wang

Animals 2022, 12(1), 106; https://doi.org/10.3390/ani12010106 - 2 Jan 2022

Cited by 28 | Viewed by 8148

Abstract

This review presents and analyzes recent scientific findings on the structure, physiology, and neurotransmission mechanisms of transient receptor potential (TRP) and their function in the thermoregulation of mammals. The aim is to better understand the functionality of these receptors and their role in [...] Read more.

This review presents and analyzes recent scientific findings on the structure, physiology, and neurotransmission mechanisms of transient receptor potential (TRP) and their function in the thermoregulation of mammals. The aim is to better understand the functionality of these receptors and their role in maintaining the temperature of animals, or those susceptible to thermal stress. The majority of peripheral receptors are TRP cation channels formed from transmembrane proteins that function as transductors through changes in the membrane potential. TRP are classified into seven families and two groups. The data gathered for this review include controversial aspects because we do not fully know the mechanisms that operate the opening and closing of the TRP gates. Deductions, however, suggest the intervention of mechanisms related to G protein-coupled receptors, dephosphorylation, and ligands. Several questions emerge from the review as well. For example, the future uses of these data for controlling thermoregulatory disorders and the invitation to researchers to conduct more extensive studies to broaden our understanding of these mechanisms and achieve substantial advances in controlling fever, hyperthermia, and hypothermia. Full article

(This article belongs to the Special Issue Thermal stress—Thermoregulatory and Adaptive Responses of Livestock)

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17 pages, 3896 KiB

Open AccessArticle

Dog Ecology and Demographics in Several Areas in the Philippines and Its Application to Anti-Rabies Vaccination Programs

by Amit Chaudhari, Tamara Kartal, George Brill, Kazami Joanne Amano, Maria Glofezita Lagayan and Daphne Jorca

Animals 2022, 12(1), 105; https://doi.org/10.3390/ani12010105 - 2 Jan 2022

Cited by 7 | Viewed by 13970

Abstract

Understanding dog population dynamics plays a vital role in planning both rabies and dog management interventions. Establishing a human to dog ratio and an understanding how the urban/rural nature of the community might affect the overall dog population estimate provides an easy-to-use reference [...] Read more.

Understanding dog population dynamics plays a vital role in planning both rabies and dog management interventions. Establishing a human to dog ratio and an understanding how the urban/rural nature of the community might affect the overall dog population estimate provides an easy-to-use reference to estimate approximate dog populations in a range of communities. A total of 10,664 households were interviewed in 10 locations in the Philippines (2017 and 2018) to understand the dog population variations among the urban, semi-urban and rural areas. Epicollect5 and OSM tracker applications were used to conduct household interviews using a predesigned fixed set of questions. All answers were recorded directly using mobile phone applications. The survey results showed that for every 1000 humans, there are 256.3 dogs in rural areas, 213.8 dogs in semi-rural areas, 208.7 dogs in urban areas and 170.0 dogs on small islands of the Philippines. We estimate a total dog population in the Philippines of 23.29 million dogs (CI 95%, 22.51–24.07 million). Based on the survey findings from Quezon City and Cebu City, targets, resources allocations and vaccination approach were adjusted for the anti-rabies vaccination program at two locations in 2018, which lead to a 3- to 4-fold increase in the total number of dogs vaccinated in each city compared to previous years. Full article

(This article belongs to the Special Issue Humane Global Dog Management)

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15 pages, 792 KiB

Open AccessArticle

Retinol and α-Tocopherol Contents, Fat Color, and Lipid Oxidation as Traceability Tools of the Feeding System in Suckling Payoya Kids

by Mercedes Roncero-Díaz, Begoña Panea, María de Guía Córdoba, Anastasio Argüello and María J. Alcalde

Animals 2022, 12(1), 104; https://doi.org/10.3390/ani12010104 - 2 Jan 2022

Cited by 2 | Viewed by 2155

Abstract

The effects of Payoya kid feeding systems on the fat-soluble vitamin (retinol/α-tocopherol) contents, fat content, fat color, and the oxidation index were evaluated to determine their potential for use as feeding system traceability tools. Four groups of Payoya kids (55 animals in total) [...] Read more.

The effects of Payoya kid feeding systems on the fat-soluble vitamin (retinol/α-tocopherol) contents, fat content, fat color, and the oxidation index were evaluated to determine their potential for use as feeding system traceability tools. Four groups of Payoya kids (55 animals in total) fed milk exclusively were studied: a group fed a milk replacer (MR) and three groups fed natural milk from dams reared with different management systems (mountain grazing (MG), cultivated meadow (CM) and total mixed ration (TMR)). Kids were slaughtered around one month of age and 8 kg of live weight. Kids from the MG and CM groups presented lower retinol (5.56 and 3.72 µg/mL) and higher α-tocopherol plasma (11.43 and 8.85 µg/mL) concentrations than those from the TMR and MR groups (14.98 and 22.47 µg/mL of retinol; 2.49 and 0.52 µg/mL of α-tocopherol, respectively) (p < 0.001). With respect to fat, kids with a higher intramuscular fat percentage (CM and TMR groups) had lower retinol contents (16.52 and 15.99 µg/mL, respectively) than kids from the MG and MR groups (26.81 and 22.63 µg/mL, respectively) (p < 0.001). A dilution effect of vitamins on fat was shown: the higher the amount of fat, the lower the vitamin concentrations, the higher the lipid oxidation index (MDA), and the lower the SUM (absolute value of the integral of the translated spectra between 450 and 510 nm). A discriminant analysis that included all studied variables showed that 94.4% of the kids were classified correctly according to their feeding system and could allow traceability to the consumer. Full article

(This article belongs to the Special Issue Competitiveness of Spanish Local Breeds)

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3 pages, 261 KiB

Open AccessEditorial

It’s Animals’ Ten Year Anniversary

by Clive J. C. Phillips

Animals 2022, 12(1), 103; https://doi.org/10.3390/ani12010103 - 2 Jan 2022

Viewed by 1691

Abstract

About 30 years ago I had a discussion with my then head of department at Bangor University, the late Professor John Bryn Owen, about what an ideal journal would look like in our field, animal science, in the future [...] Full article

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12 pages, 2288 KiB

Open AccessArticle

An Opportunistic Assessment of the Impact of Squirrelpox Disease Outbreaks upon a Red Squirrel Population Sympatric with Grey Squirrels in Wales

by Craig M. Shuttleworth, David Everest, Paul Holmes, Suzi Bell and Rachel Cripps

Animals 2022, 12(1), 99; https://doi.org/10.3390/ani12010099 - 1 Jan 2022

Cited by 9 | Viewed by 4095

Abstract

Native red squirrels (Sciurus vulgaris) persisted in the coastal mainland woodlands of northern Gwynedd whilst sympatric with an invasive grey squirrel (Sciurus carolinensis) population suppressed by culling. Squirrelpox disease in the red squirrel population was recorded in 2017 and [...] Read more.

Native red squirrels (Sciurus vulgaris) persisted in the coastal mainland woodlands of northern Gwynedd whilst sympatric with an invasive grey squirrel (Sciurus carolinensis) population suppressed by culling. Squirrelpox disease in the red squirrel population was recorded in 2017 and 2020/21. An autumn 2020 outbreak was associated with only 17.4% of animals caught and marked in the preceding June known to be present in March 2021. Despite an opportunistic data collection lacking the rigour of empirical experimental design, we observed low local survival rates similar to previously published accounts reported during major squirrelpox outbreaks. The use of a conservation dog to detect red squirrel carcasses resulted in positive detection and confirmation of a temporal and spatial expansion of one disease outbreak. The study is the first in Wales to use conservation dogs and the findings reinforce the vital strategic importance of geographical isolation reducing sympatry of red with grey squirrels in European regions where the introduced congener is a source of the squirrelpox infection. Full article

(This article belongs to the Special Issue Wildlife Disease Monitoring: Methods and Perspectives)

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11 pages, 1724 KiB

Open AccessArticle

Genetic Variability Trend of Lusitano Horse Breed Reared in Italy

by Maria Cristina Cozzi, Paolo Valiati, Maria Longeri, Carlos Ferreira and Sofia Abreu Ferreira

Animals 2022, 12(1), 98; https://doi.org/10.3390/ani12010098 - 1 Jan 2022

Cited by 4 | Viewed by 2824

Abstract

The Lusitano Horse (LH) originates from Portugal, but is reared worldwide. Since 1994, the University of Milan has routinely tested the LHs bred in Italy for parentage control. This study aims to assess the genetic variability of the LH reared in Italy using [...] Read more.

The Lusitano Horse (LH) originates from Portugal, but is reared worldwide. Since 1994, the University of Milan has routinely tested the LHs bred in Italy for parentage control. This study aims to assess the genetic variability of the LH reared in Italy using 16 microsatellites markers. Moreover, the genetic variability changes over the years in the total population (n.384) and in unrelated horses (n.47) were evaluated. Horses were grouped according to their date of birth (1975–1990, 1991–2000, 2001–2010, 2010–2019). Standard genetic diversity parameters, including observed (Ho) and expected (He) heterozygosity, Hardy-Weinberg equilibrium (HWE; P-Val), allelic richness, and inbreeding coefficient (F_is) were estimated. In the whole period, the total population showed Ho as high as 0.69, low F_is (0.057), and imbalance for HWE. When considering the unrelated horses, Ho was seen to increase over time (from 0.594 in 1975–1990 to 0.68 in 2010–2019) and frequencies were in HWE, again having low and decreasing values of Fis (from 0.208 in 1975–1990 to 0.019 in 2010–2019). Bottleneck analysis excluded a recent population decline. Principal Coordinate Analysis at the individual level defined two clusters, the major cluster including all the most recent horses. An increasing number of dams (156% more from 2001–2010 to 2011–2019) supports the good variability recorded in the population so far. However, the high number of foals (77.2%) sired by only four stallions in recent years suggests caution in the choice of the sires for the future. Full article

(This article belongs to the Collection Genetic Diversity in Livestock and Companion Animals)

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14 pages, 590 KiB

Open AccessArticle

Virulence Gene Profile, Antimicrobial Resistance and Multilocus Sequence Typing of Salmonella enterica Subsp. enterica Serovar Enteritidis from Chickens and Chicken Products

by Zunita Zakaria, Latiffah Hassan, Zawiyah Sharif, Norazah Ahmad, Rohaya Mohd Ali, Suraya Amir Husin, Norfitriah Mohamed Sohaimi, Shafini Abu Bakar and Bashiru Garba

Animals 2022, 12(1), 97; https://doi.org/10.3390/ani12010097 - 1 Jan 2022

Cited by 10 | Viewed by 3541

Abstract

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples [...] Read more.

This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of Salmonella in the Central Region of Peninsular Malaysia. A total of 45 Salmonella Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the tetC and blaTEM β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the Salmonella pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (spvB, spvC) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Salmonella Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity. Full article

(This article belongs to the Topic Animal Diseases in Agricultural Production Systems)

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11 pages, 567 KiB

Open AccessArticle

Fatty Acid Profile, Conjugated Linoleic Acid Content, and Lipid Quality Indices in Selected Yogurts Available on the Polish Market

by Beata Paszczyk and Marta Czarnowska-Kujawska

Animals 2022, 12(1), 96; https://doi.org/10.3390/ani12010096 - 1 Jan 2022

Cited by 12 | Viewed by 2899

Abstract

The aim of the present study was to determine the fatty acid composition, the content of cis9trans11 C18:2 acid (CLA), and lipid quality indices in yogurts made of cow’s milk, available on the Polish market. The test material consisted of: [...] Read more.

The aim of the present study was to determine the fatty acid composition, the content of cis9trans11 C18:2 acid (CLA), and lipid quality indices in yogurts made of cow’s milk, available on the Polish market. The test material consisted of: natural yogurts, natural yogurts with additives (muesli, cereal grains), bio yogurts, bio yogurts with additives (millet groats, quinoa, chestnuts), probiotic yogurts, and eco yogurts. All the products were bought in the period from May to June 2021. The conducted research showed that the analyzed yoghurts were characterized by a varying content of fatty acid groups, different values of the calculated lipid quality indices, as well as a different content of conjugated linoleic acid cis9trans11 C18: 2 (CLA). Natural yogurts with additives had the highest content of polyunsaturated fatty acids (PUFAs) and n-3 PUFAs. Natural and bio yogurts with additives had a higher content of n-6 PUF than the other analyzed yogurts. The n-6/n-3 ratio was lower in bio yogurts and eco yogurts. Natural yogurts with additives featured the lowest index of atherogenicity (AI) and index of thrombogenicity (TI) and the highest hypocholesterolemic/hypercholesterolemic ratio (H/H). The fat extracted from the bio yogurts had the highest (0.90% of total fatty acids) mean content of cis9trans11 C18:2 (CLA). In fat of the other analyzed yogurts, mean CLA content in total content of fatty acids varied from 0.48% in natural yogurts with additives to 0.81% in bio yogurts with additives. Full article

(This article belongs to the Special Issue Recent Advances in Fermented Milk and Meat Products—Quality, Nutritional Value and Safety)

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29 pages, 488 KiB

Open AccessReview

Non-Antibiotics Strategies to Control Salmonella Infection in Poultry

by José Martín Ruvalcaba-Gómez, Zuamí Villagrán, Juan José Valdez-Alarcón, Marcelino Martínez-Núñez, Lorena Jacqueline Gomez-Godínez, Edmundo Ruesga-Gutiérrez, Luis Miguel Anaya-Esparza, Ramón Ignacio Arteaga-Garibay and Angélica Villarruel-López

Animals 2022, 12(1), 102; https://doi.org/10.3390/ani12010102 - 1 Jan 2022

Cited by 54 | Viewed by 12904

Abstract

Salmonella spp. is a facultative intracellular pathogen causing localized or systemic infections, involving economic and public health significance, and remains the leading pathogen of food safety concern worldwide, with poultry being the primary transmission vector. Antibiotics have been the main strategy for Salmonella [...] Read more.

Salmonella spp. is a facultative intracellular pathogen causing localized or systemic infections, involving economic and public health significance, and remains the leading pathogen of food safety concern worldwide, with poultry being the primary transmission vector. Antibiotics have been the main strategy for Salmonella control for many years, which has allowed producers to improve the growth and health of food-producing animals. However, the utilization of antibiotics has been reconsidered since bacterial pathogens have established and shared a variety of antibiotic resistance mechanisms that can quickly increase within microbial communities. The use of alternatives to antibiotics has been recommended and successfully applied in many countries, leading to the core aim of this review, focused on (1) describing the importance of Salmonella infection in poultry and the effects associated with the use of antibiotics for disease control; (2) discussing the use of feeding-based (prebiotics, probiotics, bacterial subproducts, phytobiotics) and non-feeding-based (bacteriophages, in ovo injection, vaccines) strategies in poultry production for Salmonella control; and (3) exploring the use of complementary strategies, highlighting those based on -omics tools, to assess the effects of using the available antibiotic-free alternatives and their role in lowering dependency on the existing antimicrobial substances to manage bacterial infections in poultry effectively. Full article

(This article belongs to the Special Issue Strategies to Control Foodborne Pathogens: Pre- and Post-harvest Safety of Animal Food Products)

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8 pages, 636 KiB

Open AccessReview

Minireview: Peripheral Nesfatin-1 in Regulation of the Gut Activity—15 Years since the Discovery

by Katarzyna Kras, Siemowit Muszyński, Ewa Tomaszewska and Marcin B. Arciszewski

Animals 2022, 12(1), 101; https://doi.org/10.3390/ani12010101 - 1 Jan 2022

Cited by 9 | Viewed by 2250

Abstract

Nesfatin-1, discovered in 2006, is an anorexigenic molecule derived from the precursor protein NEFA/nucleobindin2. It is generally postulated that this molecule acts through a specific G protein-coupled receptor, as yet unidentified. Research conducted over the last 15 years has revealed both central and [...] Read more.

Nesfatin-1, discovered in 2006, is an anorexigenic molecule derived from the precursor protein NEFA/nucleobindin2. It is generally postulated that this molecule acts through a specific G protein-coupled receptor, as yet unidentified. Research conducted over the last 15 years has revealed both central and peripheral actions of nesfatin-1. Given its major central role, studies determining its inhibitory effect on food intake seem to be of major scientific interest. However, in recent years a number of experiments have found that peripheral organs, including those of the gastrointestinal tract (GIT), may also be a source (possibly even the predominant source) of nesfatin-1. This mini-review aimed to summarize the current state of knowledge regarding the expression and immunoreactivity of nesfatin-1 and its possible involvement (both physiological and pathological) in the mammalian GIT. Research thus far has shown very promising abilities of nesfatin-1 to restore the balance between pro-oxidants and antioxidants, to interplay with the gut microbiota, and to alter the structure of the intestinal barrier. This necessitates more extensive research on the peripheral actions of this molecule. More in-depth knowledge of such mechanisms (especially those leading to anti-inflammatory and anti-apoptotic effects) is important for a better understanding of the involvement of nefatin-1 in GIT pathophysiological conditions and/or for future therapeutic approaches. Full article

(This article belongs to the Special Issue Microscopic Structure Research in Animals)

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