Designing a Waste-Based Culture Medium for the Production of Plant Growth Promoting Microorganisms Based on Cladodes Juice from Opuntia ficus-indica Pruning
, Mario Trupo 1,*, Alfredo Ambrico 1,*, Vincenzo Larocca 1, Maria Martino 1, Salvatore Palazzo 1, Roberto Balducchi 1, Vesa Joutsjoki 2, Anne Pihlanto 2 and Annamaria Bevivino 3Round 1
Reviewer 1 Report
This is a very interesting article, which is of value for the scientific community as well as the industry. some of the discussion parts are a little superficial and not focussed. The research is closely related to the work published by Cantabella et al. 2021. A paragraph on the actual cost/energy/carbon footprint should be added in comparison to the current processes.
Is there any secondary metabolites in the juice which could be negative to the growth of the microorganisms?
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
I think, this is an quite good manuscript. The results of this study provide new knowledge for scientists and for practitioners. These results bring some knowledge of the given topic. Major corrections should be made and it should be re-reviewed:
- Introduction part is very poor. It should be added why this topic is important. What does the presented research bring to science? What is different from previously published?
- The Results and Discussion section requires expansion. Mention should be made of other options for the treatment or use of waste of various origins. There are many studies involving microorganisms, for example:
Impact of calcium oxide on hygienization and self-heating prevention of biologically contaminated polymer materials.
Energy-related and microbiological evaluation of the effects of bulking agents on the brewery hot trub biodrying.
Biological activity of composts obtained from hop waste generated during the brewing.
Bio-based synthesis of silver nanoparticles from waste agricultural biomass and its antimicrobial activity.
The application potential of hop sediments from beer production for composting.
and should be cited in the text.
- Extensive editing of English language and style required. - Please indicate the amendments in the text
Author Response
Please see the attachment
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Amendments have been made, can be accepted.
