Feature Papers in Applied Biosciences 2024

Special Issue Editors


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Guest Editor
1. Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, QLD 4072, Australia
2. ARC Centre of Excellence for Plant Success in Nature and Agriculture, University of Queensland, Brisbane, QLD 4072, Australia
Interests: genomics; transcriptomics; plant adaptation; wild crop relatives; output traits
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Guest Editor
Department of Chemistry and Chemical Biology, College of Sciences, Northeastern University, Boston, MA 02115, USA
Interests: microbial ecology; biotechnology; growth kinetics and stoichiometry; fermentation; mathematical models of microbial growth; genome-scale metabolic reconstructions; history of microbiology
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Guest Editor
Faculty of Medicine, Department Medical Physics, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece
Interests: optical methods for tissue diagnostics; bio-molecular spectroscopy; x-ray diffraction; computational biophysics and drug design; molecular modeling
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Many advances in technologies underpinning biosciences have accelerated the potential for the application of novel approaches to the major challenges of human health and well-being. It is important that those developing these technologies connect with those looking to apply them to deliver new solutions. Applied Biosciences (ISSN 2813-0464) has the opportunity to contribute to this knowledge exchange.

We are pleased to announce this Special Issue focusing on new advances in the biotechnology, biosciences and bioengineering of plants, animals and humans.

We welcome the submission of manuscripts from Editorial Board Members and from outstanding scholars invited by the Editorial Board Members and the Editorial Office. The following are suggested (as examples of options) as submission topics:

  • Advances in drug discovery;
  • Microscopy and molecular imaging technology developments;
  • Advances in antibody engineering;
  • Novel bioassay technologies;
  • New bioinformatics tools;
  • Recent applications of genome engineering in plants or animals;
  • Cell engineering;
  • Enzyme engineering;
  • Applications of nanobiotechnology;
  • Advances in the production and use of biopolymers;
  • Novel vaccine technologies/mRNA vaccines;
  • Algal products and production systems;
  • Advances in long-read DNA sequencing;
  • Biomass processing technologies;
  • Production of biomaterials and biofuels;
  • Advanced biomanufacturing;
  • Microbial biotechnology.

We hope you can participate in this Special Issue and look forward to receiving your contributions.

Prof. Dr. Robert Henry
Dr. Nicolai S. Panikov
Dr. Nikolaos Kourkoumelis
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Biosciences is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • advances in drug discovery
  • microscopy and molecular imaging technology developments
  • advances in antibody engineering
  • novel bioassay technologies
  • new bioinformatics tools
  • recent applications of genome engineering in plants or animals
  • cell engineering
  • enzyme engineering
  • applications of nanobiotechnology
  • advances in the production and use of biopolymers
  • novel vaccine technologies/mRNA vaccines
  • algal products and production systems
  • advances in long-read DNA sequencing
  • biomass processing technologies
  • production of biomaterials and biofuels
  • advanced biomanufacturing
  • microbial biotechnology

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Related Special Issue

Published Papers (5 papers)

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Research

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19 pages, 4092 KiB  
Article
Effect of Integrated Crop–Livestock Systems on Soil Properties and Microbial Diversity in Soybean Production
by Namita Sinha, Brett R. Rushing, Aniruddha Acharya and Shankar Ganapathi Shanmugam
Appl. Biosci. 2024, 3(4), 484-502; https://doi.org/10.3390/applbiosci3040031 - 8 Nov 2024
Viewed by 428
Abstract
Integrated crop and livestock systems (ICLSs) have been considered an important management-based decision to improve soil health by carbon sequestration. A two-year study (2019–2021) at CPBES in Newton, MS, was conducted to evaluate the effect of an ICLS on soil microbial diversity in [...] Read more.
Integrated crop and livestock systems (ICLSs) have been considered an important management-based decision to improve soil health by carbon sequestration. A two-year study (2019–2021) at CPBES in Newton, MS, was conducted to evaluate the effect of an ICLS on soil microbial diversity in the southeastern region of the USA, representing agroclimatic conditions that are warm and humid. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and characterization of microbial diversity were performed using QIIME2®. Soil fungal diversity pattern showed significant difference (alpha diversity, p = 0.031 in 2020 and beta diversity, p = 0.037 in 2021). In contrast, no significant differences were observed in bacterial diversity. However, there were several beneficial bacterial phyla, such as Proteobacteria and Actinobacteria, and fungal phyla such as Ascomycota, which were dominant in both years and did not show significant differences due to cover crop treatments. Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to carbon (rm = 0.2581, p = 0.022), nitrogen (rm = 0.2921, p = 0.0165), and electrical conductivity (rm = 0.1836, p = 0.0583) in 2021, and on bacterial diversity due to EE-GRSP (rm = 0.22, p = 0.02) in 2020. However, the results showed that there were no significant differences between the cover crop treatments that were consistent over a two-year study period. However, the mix of different cover crops such as oats (Avena sativa L.), crimson clover (Trifolium incarnatum L.), and tillage radish (Raphanus sativus L.) demonstrated higher positive correlation and lower negative correlation with different bacterial and fungal phyla. Long term study of ICLS is suggested to understand the shift in microbiome that would help in understanding the role of cover crops and grazing in improving crop production sustainably. Full article
(This article belongs to the Special Issue Feature Papers in Applied Biosciences 2024)
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13 pages, 2694 KiB  
Article
Orthotic Thermoplastic Demonstrates a Similar Contamination Potential with Bacillus Bacteria Recovered from Thermoplastic Radiation Therapy Patient Masks
by Catherine W. Brock, Dev V. Mehta and Terrence J. Ravine
Appl. Biosci. 2024, 3(2), 250-262; https://doi.org/10.3390/applbiosci3020017 - 1 Jun 2024
Viewed by 789
Abstract
Thermoplastics used to construct a variety of patient medical devices can become contaminated by harmful bacteria. We investigated whether two different Bacillus species recovered from patient radiation therapy thermoplastic masks could similarly contaminate thermoplastic material used to construct patient orthoses (splints). Bacillus bacteria [...] Read more.
Thermoplastics used to construct a variety of patient medical devices can become contaminated by harmful bacteria. We investigated whether two different Bacillus species recovered from patient radiation therapy thermoplastic masks could similarly contaminate thermoplastic material used to construct patient orthoses (splints). Bacillus bacteria form dormant spores, which have been shown to enhance its attachment to thermoplastics. Bacterial attachment and recovery were examined using an orthotic thermoplastic with an anti-stick coating being compared to uncoated material used in radiation therapy applications. Triplicate sample squares were seeded with a saline suspension of either B. cereus (MAB03F) or B. megaterium (DAB01F) containing a similar number of spores. Squares were subsequently sampled at 1 h, 1 week, 2 weeks, 4 weeks, and 8 weeks. The number of recovered bacteria was counted. Differences in material hydrophobicity were determined by water contact angle analysis. Both Bacillus species attached to each material within 1 h, and their spores were recovered at 8 weeks. However, a decreasing trend in adhesion, over time, was noted to the coated material with an opposite increasing trend in the uncoated material. Decreased Bacillus species spore adhesion to coated material with a lower hydrophobicity suggests a greater potential for spore transfer to patients wearing contaminated orthoses. Full article
(This article belongs to the Special Issue Feature Papers in Applied Biosciences 2024)
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Review

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14 pages, 642 KiB  
Review
A Short Review of Second-Generation Isobutanol Production by SHF and SSF
by Hironaga Akita and Akinori Matsushika
Appl. Biosci. 2024, 3(3), 296-309; https://doi.org/10.3390/applbiosci3030020 - 8 Jul 2024
Viewed by 989
Abstract
As isobutanol exhibits higher energy density and lower hygroscopicity than ethanol, it is considered a better candidate biofuel. The sustainable supply of inedible biomass and lack of competition with the food supply have stimulated significant worldwide interest in the production of isobutanol from [...] Read more.
As isobutanol exhibits higher energy density and lower hygroscopicity than ethanol, it is considered a better candidate biofuel. The sustainable supply of inedible biomass and lack of competition with the food supply have stimulated significant worldwide interest in the production of isobutanol from this resource. Both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) have been applied to isobutanol production to effectively utilize inedible biomass as a feedstock. However, both processes have various challenges, including low isobutanol yield and high production costs. This review summarizes the potential of isobutanol as a biofuel, methods for conferring isobutanol productivity, recent experimental studies, and developments in both SHF and SSF with the isobutanol-producing strains. Challenges to increasing the isobutanol yield and various suggestions for improvements to enable commercial production are also discussed. Full article
(This article belongs to the Special Issue Feature Papers in Applied Biosciences 2024)
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20 pages, 1576 KiB  
Review
Sustainable Pulse Proteins: Physical, Chemical and Fermentative Modifications
by Seedhabadee Ganeshan, Nancy Asen, Yingxin Wang, Mehmet Ç. Tülbek and Michael T. Nickerson
Appl. Biosci. 2024, 3(2), 263-282; https://doi.org/10.3390/applbiosci3020018 - 12 Jun 2024
Viewed by 2056
Abstract
Pulse proteins are playing significant roles in the alternative protein space due to the demand for foods produced in an environmentally sustainable manner and, most importantly, due to the demand for foods of nutritious value. There has been extensive research to mimic animal-derived [...] Read more.
Pulse proteins are playing significant roles in the alternative protein space due to the demand for foods produced in an environmentally sustainable manner and, most importantly, due to the demand for foods of nutritious value. There has been extensive research to mimic animal-derived meat texture, flavour, mouthfeel, etc. However, there is still the perception that many of the plant-based proteins that have been texturized to mimic meat are still highly processed and contain chemicals or preservatives, reducing their appeal as being healthy and precluding any sustainable benefits. To counter this notion, the biotransformation of pulse proteins using enzymes or fermentation offers unique opportunities. Thus, this review will address the significance of pulse proteins in the alternative protein space and some of the processing aids leading to the isolation and modification of such protein concentrates in a sustainable manner. Fermentation-based valorization of pulse proteins will also be discussed as a “clean label” strategy (further adding to sustainable nutritious plant protein production), although some of the processes like the extensive use of water in submerged fermentation need to be addressed. Full article
(This article belongs to the Special Issue Feature Papers in Applied Biosciences 2024)
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16 pages, 4320 KiB  
Review
Moringa oleifera Seed Cake: A Review on the Current Status of Green Nanoparticle Synthesis
by Nuno Coelho, Alice S. Pereira and Pedro Tavares
Appl. Biosci. 2024, 3(2), 197-212; https://doi.org/10.3390/applbiosci3020013 - 29 Apr 2024
Viewed by 1113
Abstract
Growing demands for sustainable and ecological nanoparticle synthesis methods have incentivized the scientific community to develop new approaches to counteract these challenges. Green synthesis resorts to biocomponents obtained from plants, bacteria, fungi, and other organisms to synthesize nanostructures, with beneficial gains in the [...] Read more.
Growing demands for sustainable and ecological nanoparticle synthesis methods have incentivized the scientific community to develop new approaches to counteract these challenges. Green synthesis resorts to biocomponents obtained from plants, bacteria, fungi, and other organisms to synthesize nanostructures, with beneficial gains in the economic and ecological cost associated with the process, simplicity of the process, and resource efficiency. Moringa oleifera, a native plant originally from India with immense nutritive value, has long been used by researchers in the biosynthesis of nanoparticles. Leaves, flowers, bark, and seeds are among the “miracle tree” parts that can be used in nanoparticle green synthesis. Moringa oleifera seed cake, a by-product obtained from defatted seeds, is often overlooked due to its apparent low commercial value. The main objective of this review is to highlight the recent findings reported in the literature on nanoparticles/nanocomposites synthesized with seed cake biocompounds acting as reducing/capping agents. Furthermore, we analyzed the methods currently employed for the extraction of bioactive compounds. Moringa oleifera seed for industrial applications was also addressed. Full article
(This article belongs to the Special Issue Feature Papers in Applied Biosciences 2024)
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