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Biosensors
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Electrochemical Biosensing Platforms for Food, Drug and Health Safety
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Electrochemical Biosensing Platforms for Food, Drug and Health Safety

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 6389

Special Issue Editor

Dr. Mihaela Puiu

E-Mail Website
Guest Editor
Department of Physical Chemistry University of Bucharest, 030018-Bucharest, Romania
Interests: electrochemical biosensors; surface plasmon resonance biosensors; homogenous catalysis; reaction kinetics; self-assembled monolayers; surface coatings
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Electrochemical platforms are emerging as powerful tools for various applications in the fields of food, drug, and health. These platforms rely on the principles of electrochemistry to detect and quantify target analytes in complex matrices such as food, drugs, and biological fluids. They offer several advantages, such as high sensitivity, selectivity, and a rapid response time. In the food industry, electrochemical platforms can be used for detecting contaminants, such as pesticides, heavy metals, and foodborne pathogens, ensuring the safety and quality of food products. In the food industry, electrochemical platforms can be used for detecting contaminants, such as pesticides, heavy metals, and foodborne pathogens, ensuring the safety and quality of food products. In the pharmaceutical industry, electrochemical platforms can be used for drug discovery, drug delivery, and monitoring drug efficacy. In the healthcare industry, electrochemical platforms can be used for disease diagnosis, monitoring biomarkers, and personalized medicine. Electrochemical platforms based on organic–inorganic hetero-nano-interfaces represent a promising avenue in the development of highly sensitive and selective biosensors. These platforms combine the advantages of organic and inorganic components, resulting in improved electrical conductivity, biocompatibility, and selectivity. Additionally, the use of organic materials in these interfaces allows for the design of functional groups that can selectively bind to target molecules, further increasing the selectivity of the sensor.

We invite contributions to our upcoming Special Issue about electrochemical biosensing platforms for food, drug, and health safety. We welcome submissions from a diverse range of fields pertaining to this topic, with particular interest in studies concerning the design, development, and application of organic–inorganic hetero-nano-interfaces in portable and miniaturized devices for the rapid detection of contaminants and pathogens.

Dr. Mihaela Puiu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemical bio(immuno)sensing
  • organic–inorganic hetero-nano-interfaces
  • early detection, signal amplification,
  • conductive nanocomposites
  • magnetic nanoparticles
  • nanozymes

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Published Papers (4 papers)

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20 pages, 7800 KiB  
Article
Portable Miniaturized IoT-Enabled Point-of-Care Device for Electrochemical Sensing of Zopiclone in Cocktails
by María Gabriela Mejía-Méndez, Paula C. Cifuentes-Delgado, Sergio D. Gómez, Crhistian C. Segura, Nancy Ornelas-Soto and Johann F. Osma
Biosensors 2024, 14(11), 557; https://doi.org/10.3390/bios14110557 - 16 Nov 2024
Viewed by 511
Abstract
This study proposes a portable and IoT-based electrochemical point-of-care sensing device for detecting zopiclone in cocktails. The system utilizes an electrochemical laccase biosensor and a potentiostat, offering a low-cost and portable device for detecting this sedative drug in cocktails. The sensor characterization experiments [...] Read more.
This study proposes a portable and IoT-based electrochemical point-of-care sensing device for detecting zopiclone in cocktails. The system utilizes an electrochemical laccase biosensor and a potentiostat, offering a low-cost and portable device for detecting this sedative drug in cocktails. The sensor characterization experiments demonstrated the linear behavior of the oxidation and reduction currents for each of the targeted concentrations of zopiclone, enabling their detection and quantification even when mixed with an interfering substance. The proposed system could be used for the in situ analysis of cocktails, providing a valuable tool for monitoring the presence of hypnotic drugs in various social and clinical settings. The study utilized materials and reagents, including zopiclone, lab-made lemon juice, lab-made tequila, and lab-made triple sec, all prepared with reactants obtained in Bogotá, Colombia. The potentiostat used in the system was designed to manage cyclic voltammetry measurements. The electrochemical cells’ durability and longevity were also tested and characterized, with all electrodes undergoing 200 tests and their performance degradation varying according to the molecule used. The study concludes that the proposed system offers a valuable tool for detecting and monitoring pharmaceutical substances in various interfering ingredients that build up cocktails. Further research and application of this system can help address the global concern surrounding the administration of hypnotic substances to unknowing consumers through food or drinks to enable robbery and sexual assault. Full article
(This article belongs to the Special Issue Electrochemical Biosensing Platforms for Food, Drug and Health Safety)
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15 pages, 6864 KiB  
Article
Advanced Electrochemical Monitoring of Carbendazim Fungicide in Foods Using Interfacial Superassembly of NRPC/NiMn Frameworks
by Shakila Parveen Asrafali, Thirukumaran Periyasamy, Seong Cheol Kim and Jaewoong Lee
Biosensors 2024, 14(10), 474; https://doi.org/10.3390/bios14100474 - 2 Oct 2024
Viewed by 813
Abstract
A simple, sensitive and reliable sensing system based on nitrogen-rich porous carbon (NRPC) and transition metals, NRPC/Ni, NRPC/Mn and NRPC/NiMn was developed and successfully applied as electrode materials for the quantitative determination of carbendazim (CBZ). The synergistic effect of NRPC and bimetals with [...] Read more.
A simple, sensitive and reliable sensing system based on nitrogen-rich porous carbon (NRPC) and transition metals, NRPC/Ni, NRPC/Mn and NRPC/NiMn was developed and successfully applied as electrode materials for the quantitative determination of carbendazim (CBZ). The synergistic effect of NRPC and bimetals with acceptable pore structure together with flower-like morphology resulted in producing a highly conductive and interconnected network in NRPC/NiMn@GCE, which significantly enhanced the detection performance of CBZ. The electrochemical behavior investigated by cyclic voltammetry (CV) showed improved CBZ detection for NRPC/NiMn, due to the controlled adsorption/diffusion process of CBZ by the NRPC/NiMn@GCE electrode. The influences of various factors such as pH, NRPC/NiMn concentration, CBZ concentration and scan rate were studied. Under optimal conditions, 0.1 M phosphate-buffered saline (PBS) with a pH of 7.0 containing 30 µg/mL NRPC/NiMn, a favourable linear range detection of CBZ from 5 to 50 µM was obtained. Moreover, a chronoamperometric analysis showed excellent repeatability, reproducibility and anti-interfering ability of the fabricated NRPC/NiMn@GCE sensor. Furthermore, the sensor showed satisfactory results for CBZ detection in real samples with acceptable recoveries of 96.40–104.98% and low RSD values of 0.25–3.45%. Full article
(This article belongs to the Special Issue Electrochemical Biosensing Platforms for Food, Drug and Health Safety)
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14 pages, 3709 KiB  
Article
An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics
by Mei Xie, Tianlan Chen, Zongwei Cai, Bo Lei and Cheng Dong
Biosensors 2024, 14(1), 50; https://doi.org/10.3390/bios14010050 - 18 Jan 2024
Cited by 3 | Viewed by 2364
Abstract
Recently, significant progress has been made in the development of microdevices for point-of-care infectious disease detection. However, most microdevices only allow limited steps, such as DNA amplification on the chip, while sample preparation, such as lysis and DNA extraction, is conducted off the [...] Read more.
Recently, significant progress has been made in the development of microdevices for point-of-care infectious disease detection. However, most microdevices only allow limited steps, such as DNA amplification on the chip, while sample preparation, such as lysis and DNA extraction, is conducted off the chip using the traditional method. In this study, an all-in-one platform was developed, which incorporated all necessary procedures for nucleic acid detection. Our on-chip DNA extraction method utilized the magnetic bead-based technology on a hybrid channel-digital microfluidics (C-DMF) microdevice. It yielded high recovery rates, varying from 88.43% to 95.83%, with pathogen concentrations of 103–106 CFU/mL. In particular, the on-chip method exhibited significantly higher efficacy compared to the traditional off-chip manual method, for the DNA extraction of E. coli and S. aureus, representing Gram-negative and Gram-positive bacteria, respectively, at a sample concentration of 103 CFU/mL. To address the need for rapid and accessible diagnostics, colorimetric LAMP amplification was integrated into the proposed microdevice. The results were visually detectable with the naked eye, making it user-friendly for non-specialists. In addition, this platform demonstrated impressive sensitivity in simultaneously detecting common foodborne pathogens in spiked meat samples, achieving the LOD of 102–103 CFU/mL. The entire process, from sampling to result, was fully automated and only required approximately 60 min, offering promising applicability in resource-limited and on-site testing scenarios. Full article
(This article belongs to the Special Issue Electrochemical Biosensing Platforms for Food, Drug and Health Safety)
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23 pages, 4869 KiB  
Article
A Facile Glycerol-Assisted Synthesis of Low-Cu2+-Doped CoFe2O4 for Electrochemical Sensing of Acetaminophen
by José Guillermo Alfonso-González, Claudia Patricia Granja-Banguera, Jimmy Alexander Morales-Morales and Andrés Dector
Biosensors 2023, 13(12), 997; https://doi.org/10.3390/bios13120997 - 23 Nov 2023
Cited by 2 | Viewed by 2046
Abstract
This work devised a simple glycerol-assisted synthesis of a low-Cu2+-doped CoFe2O4 and the electrochemical detection of acetaminophen (AC). During the synthesis, several polyalcohols were tested, indicating the efficiency of glycerin as a cosolvent, aiding in the creation of [...] Read more.
This work devised a simple glycerol-assisted synthesis of a low-Cu2+-doped CoFe2O4 and the electrochemical detection of acetaminophen (AC). During the synthesis, several polyalcohols were tested, indicating the efficiency of glycerin as a cosolvent, aiding in the creation of electrode-modifier nanomaterials. A duration of standing time (eight hours) before calcination produces a decrease in the secondary phase of hematite. The synthesized material was used as an electrode material in the detection of AC. In acidic conditions (pH 2.5), the limit of detection (LOD) was 99.4 nM, while the limit of quantification (LOQ) was found to be (331 nM). The relative standard deviation (RSD), 3.31%, was computed. The enhanced electrocatalytic activity of a low-Cu2+-doped CoFe2O4-modified electrode Cu0.13Co0.87Fe2O4/GCE corresponds extremely well with its resistance Rct, which was determined using the electrochemical impedance spectroscopy (EIS) technique and defined its electron transfer capacity. The possibility of a low-Cu2+-doped CoFe2O4 for the electrochemical sensing of AC in human urine samples was studied. The recovery rates ranging from 96.5 to 101.0% were obtained. These findings suggested that the Cu0.13Co0.87Fe2O4/GCE sensor has outstanding practicability and could be utilized to detect AC content in real complex biological samples. Full article
(This article belongs to the Special Issue Electrochemical Biosensing Platforms for Food, Drug and Health Safety)
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