CRISPR-Powered Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 2496

Special Issue Editors

Department of Industrial and Systems Engineering, Rochester Institute of Technology, Rochester, NY 14623, USA
Interests: 3D printing; molecular diagnostics; nucleic acid detection; biosensing
Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China
Interests: enzyme engineering and synthetic biology for molecular diagnostics; isothermal nucleic acid amplification and biosensors; rapid detection systems and all-in-one microfluidic diagnostics
Special Issues, Collections and Topics in MDPI journals
School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
Interests: 3D printing; molecular diagnostics; nucleic acid detection; biosensing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

CRISPR/Cas technology won the Nobel Prize in Chemistry in 2020 for its outstanding contribution to gene editing. In addition to its application in gene editing, a large literature shows that CRISPR/Cas technology has outstanding advantages in biosensing, with high accuracy, good specificity, and fast response. More importantly, CRISPR technology is promising not only in its application to nucleic acid detection but also in the detection of other kinds of biomarkers, including heavy metal ions, small molecules, peptides, and proteins, posing a great application potential for next-generation diagnostics, agriculture, and environmental monitoring. Therefore, this Special Issue aims to present cutting-edge fundamental and applied research activities in CRISPR-driven biosensing, showcasing innovative technologies, methods, and novel integrated devices for next-generation diagnostics, agriculture, and environmental monitoring. We invite submissions that will help to advance research in the field of CRISPR-driven biosensing technologies.

Dr. Zhiheng Xu
Prof. Dr. Xiong Ding
Dr. Kun Yin
Guest Editors

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Keywords

  • DNA
  • RNA
  • biosensing
  • nucleic acid detection
  • diagnostics
  • Cas9
  • Cas12
  • Cas13
  • CRISPR-Cas

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Published Papers (1 paper)

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Research

13 pages, 3047 KiB  
Article
A CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V
by Namki Kwak, Bum Ju Park and Yoon-Jae Song
Biosensors 2023, 13(8), 769; https://doi.org/10.3390/bios13080769 - 28 Jul 2023
Cited by 1 | Viewed by 1677
Abstract
The Japanese encephalitis virus (JEV) is prevalent in Asian countries, including Korea, Japan, China, Vietnam, and India. JEV is transmitted to humans by Culex mosquitoes. Despite extensive research efforts, no approved antiviral agents are currently available, although JE can be prevented by vaccination. [...] Read more.
The Japanese encephalitis virus (JEV) is prevalent in Asian countries, including Korea, Japan, China, Vietnam, and India. JEV is transmitted to humans by Culex mosquitoes. Despite extensive research efforts, no approved antiviral agents are currently available, although JE can be prevented by vaccination. DNA endonuclease-targeted CRISPR trans reporter (DETECTR) is a newly emerging CRISPR-Cas12a-based molecular diagnostic method combined with isothermal nucleic acid amplification. In this study, DETECTR with reverse transcription–recombinase polymerase amplification (RT-RPA) was effectively utilized for JEV diagnosis and detected down to 10 RNA copies for JEV genotype I (GI) and 1 × 102 copies for both GIII and GV, achieving similar sensitivity to RT-PCR while displaying no cross-reaction with other viruses. A one-tube, one-temperature format of DETECTR was further developed, and its efficiency compared with that of conventional DETECTR. Full article
(This article belongs to the Special Issue CRISPR-Powered Biosensing)
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