Point of Care Diagnostics for Infectious Disease

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 7068

Special Issue Editor


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Guest Editor
Fischell Department of Bioengineering, University of Maryland College Park, College Park, MD 20742, USA
Interests: point-of-care diagnostics; isothermal amplification; micro-total analysis systems; sample preparation; infectious disease diagnostics; extracellular vesicles; surface-enhanced Raman spectroscopy
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Special Issue Information

Dear Colleagues,

The diagnosis of infectious diseases requires rapid turnaround in order to begin immediate treatment for infected patients, limit the spread of infectious agents, and initiate contact tracing in the case of a rapidly spreading pandemic. A rapid turnaround times require point-of-care diagnostics to enable the diagnosis to be provided to the patient before the patient has left the testing site. This enables immediate treatment planning and contact tracing. While point-of-care diagnostic technology significantly improved during the COVID-19 pandemic, major needs still exist, especially for pathogens with a low quantity and pathogens in complex sample matrices. This Special Issue solicits cutting-edge research results on point-of-care diagnostics for infectious disease, with an emphasis on highly sensitive detection and integrated sample preparation, to enable sample-to-answer detection with complex samples.

Dr. Ian M. White
Guest Editor

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Keywords

  • point-of-care
  • infectious disease
  • COVID-19
  • sample preparation
  • sample-to-answer
  • microfluidics
  • lateral flow assay
  • micro-total analysis system

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Published Papers (2 papers)

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Research

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12 pages, 4944 KiB  
Article
Enhancing Sensitivity in SARS-CoV-2 Rapid Antigen Testing through Integration of a Water-Soluble Polymer Wall
by Xiuzhen Wang, Yu Wang, Huiyang Jie, Sidi Liu, Chenguang Shen and Qian Liu
Biosensors 2024, 14(6), 305; https://doi.org/10.3390/bios14060305 - 12 Jun 2024
Viewed by 1027
Abstract
Lateral flow immunoassays (LFIAs) are recognized for their practicality in homecare and point-of-care testing, owing to their simplicity, cost-efficiency, and rapid visual readouts. Despite these advantages, LFIAs typically fall short in sensitivity, particularly in detecting viruses such as SARS-CoV-2, thus limiting their broader [...] Read more.
Lateral flow immunoassays (LFIAs) are recognized for their practicality in homecare and point-of-care testing, owing to their simplicity, cost-efficiency, and rapid visual readouts. Despite these advantages, LFIAs typically fall short in sensitivity, particularly in detecting viruses such as SARS-CoV-2, thus limiting their broader application. In response to this challenge, we have innovated an approach to substantially enhance LFIA sensitivity. This involves the integration of a water-soluble dextran–methacrylate polymer wall with a 15% grafting degree positioned between the test and control lines on the LFIA strip. This novel modification significantly improved the sensitivity of the assay, achieving detection limits as low as 50 pg mL−1 and enhancing the sensitivity by 5–20-fold relative to existing LFIA kits available on the market. Furthermore, our developed LFIA kit (WSPW-LFIA) demonstrated exceptional specificity for SARS-CoV-2. Coupled with a straightforward fabrication process and robust stability, the WSPW-LFIA represents a promising advancement for real-time in vitro diagnosis across a spectrum of diseases. Full article
(This article belongs to the Special Issue Point of Care Diagnostics for Infectious Disease)
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Review

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29 pages, 1939 KiB  
Review
On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies
by Afagh Rastmanesh, Jayanta S. Boruah, Min-Seok Lee and Seungkyung Park
Biosensors 2024, 14(3), 122; https://doi.org/10.3390/bios14030122 - 24 Feb 2024
Cited by 5 | Viewed by 5267
Abstract
Bioaerosols are small airborne particles composed of microbiological fragments, including bacteria, viruses, fungi, pollens, and/or by-products of cells, which may be viable or non-viable wherever applicable. Exposure to these agents can cause a variety of health issues, such as allergic and infectious diseases, [...] Read more.
Bioaerosols are small airborne particles composed of microbiological fragments, including bacteria, viruses, fungi, pollens, and/or by-products of cells, which may be viable or non-viable wherever applicable. Exposure to these agents can cause a variety of health issues, such as allergic and infectious diseases, neurological disorders, and cancer. Therefore, detecting and identifying bioaerosols is crucial, and bioaerosol sampling is a key step in any bioaerosol investigation. This review provides an overview of the current bioaerosol sampling methods, both passive and active, as well as their applications and limitations for rapid on-site monitoring. The challenges and trends for detecting airborne microorganisms using molecular and immunological methods are also discussed, along with a summary and outlook for the development of prompt monitoring technologies. Full article
(This article belongs to the Special Issue Point of Care Diagnostics for Infectious Disease)
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