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Biosensors
Special Issues
Biomarker Biosensing: Analysis and Detection
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Biomarker Biosensing: Analysis and Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 30 April 2025 | Viewed by 3029

Special Issue Editors

Dr. Taisun Kim

E-Mail Website
Guest Editor
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Republic of Korea
Interests: supramolecular chemistry; microarray; protein chips; lateral flow strip membrane assay
Special Issues, Collections and Topics in MDPI journals
Dr. Satish Balasaheb Nimse

E-Mail Website
Guest Editor
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 24252, Gangwon-do, Republic of Korea
Interests: medicinal chemistry; bioorganic chemistry; supramolecular chemistry; biosensors; chemosensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Early detection of disease remains a cornerstone of effective healthcare, enabling timely intervention and improved patient outcomes. Biomarkers, molecules indicative of specific physiological or pathological processes, offer immense potential for disease diagnosis and monitoring. This special issue of Biosensors, will focus on cutting-edge advancements in biosensing technologies for the detection and quantification of diverse biomarkers relevant to human health. Advances in biomarker sensing hold immense promise for revolutionizing healthcare practice. By enabling early diagnosis, improved disease monitoring, and personalized treatment strategies, these technologies can significantly impact patient outcomes and healthcare costs.

We welcome original research articles, reviews, and methods papers encompassing a wide range of biomarker targets, analytical platforms, and clinical applications.

This special issue is planned to address the emerging trends and challenges in biomarker sensing with specific emphasis on:

  1. Multiplexed detection of protein,
  2. Nucleic acid (DNA, RNA based detection of diseases/infections),
  3. Biosensors: bioreceptor and transducer (Live cell imaging probes, detection of biothiols in body fluids, etc).
  4. Small molecule biomarkers in blood, saliva, urine, and other biofluids.
  5. Development of point-of-care (POC) biosensors for decentralized diagnostics and personalized medicine.
  6. Integration of novel sensing technologies, such as nanomaterials, microfluidics, and machine learning, for improving sensitivity, specificity, and clinical performance.
  7. Exploring the potential of cell imaging probes and biosensing strategies for early cancer detection.

Dr. Taisun Kim
Dr. Satish Balasaheb Nimse
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleic acids
  • protein biomarkers
  • biothiols
  • detection and quantification
  • small molecules

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Published Papers (2 papers)

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Research

14 pages, 5622 KiB  
Article
Development of a DNA-Based Lateral Flow Strip Membrane Assay for Rapid Screening and Genotyping of Six High-Incidence STD Pathogens
by Gunho Choi, Keum-Soo Song, Satish Balasaheb Nimse and Taisun Kim
Biosensors 2024, 14(5), 260; https://doi.org/10.3390/bios14050260 - 20 May 2024
Viewed by 1407
Abstract
Sexually transmitted diseases (STDs) are a global concern because approximately 1 million new cases emerge daily. Most STDs are curable, but if left untreated, they can cause severe long-term health implications, including infertility and even death. Therefore, a test enabling rapid and accurate [...] Read more.
Sexually transmitted diseases (STDs) are a global concern because approximately 1 million new cases emerge daily. Most STDs are curable, but if left untreated, they can cause severe long-term health implications, including infertility and even death. Therefore, a test enabling rapid and accurate screening and genotyping of STD pathogens is highly awaited. Herein, we present the development of the DNA-based 6STD Genotyping 9G Membrane test, a lateral flow strip membrane assay, for the detection and genotyping of six STD pathogens, including Trichomonas vaginalis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Mycoplasma genitalium. Here, we developed a multiplex PCR primer set that allows PCR amplification of genomic materials for these six STD pathogens. We also developed the six ssDNA probes that allow highly efficient detection of the six STD pathogens. The 6STD Genotyping 9G Membrane test lets us obtain the final detection and genotyping results in less than 30 m after PCR at 25 °C. The accuracy of the 6STD Genotyping 9G membrane test in STD genotyping was confirmed by its 100% concordance with the sequencing results of 120 clinical samples. Therefore, the 6STD Genotyping 9G Membrane test emerges as a promising diagnostic tool for precise STD genotyping, facilitating informed decision-making in clinical practice. Full article
(This article belongs to the Special Issue Biomarker Biosensing: Analysis and Detection)
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Graphical abstract

10 pages, 1838 KiB  
Communication
Catalytic Hairpin Assembly-Based Self-Ratiometric Gel Electrophoresis Detection Platform for Reliable Nucleic Acid Analysis
by Qiang Xi, Si-Yi Wang, Xiao-Bing Deng and Chong-Hua Zhang
Biosensors 2024, 14(5), 232; https://doi.org/10.3390/bios14050232 - 7 May 2024
Viewed by 1335
Abstract
The development of gel electrophoresis-based biodetection assays for point-of-care analysis are highly demanding. In this work, we proposed a ratiometric gel electrophoresis-based biosensing platform by employing catalytic hairpin assembly (CHA) process functions as both the signal output and the signal amplification module. Two [...] Read more.
The development of gel electrophoresis-based biodetection assays for point-of-care analysis are highly demanding. In this work, we proposed a ratiometric gel electrophoresis-based biosensing platform by employing catalytic hairpin assembly (CHA) process functions as both the signal output and the signal amplification module. Two types of nucleic acids, DNA and miRNA, are chosen for demonstration. The proposed strategy indeed provides a new paradigm for the design of a portable detection platform and may hold great potential for sensitive diagnoses. Full article
(This article belongs to the Special Issue Biomarker Biosensing: Analysis and Detection)
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