DNA Nanotechnology for Biosensing and Bioimaging

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (31 August 2021) | Viewed by 4608

Special Issue Editors


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Guest Editor
Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA
Interests: DNA nanotechnology; biosensing; Forster resonance energy transfer; drug delivery; nanoparticles

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Guest Editor
US Naval Research Laboratory, 4555 Overlook Ac SW, Washington, DC 20375, USA
Interests: bionanotechnology; nanoparticle; energy transfer; enzyme; kinetics; cell-free synthetic biology; biocatalysis; bioconjugation
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Special Issue Information

Dear Colleagues,

Synthetic DNA nanostructures, assembled from a few to hundreds of orthogonal oligonucleotides, have proven to be chemically addressable and highly programmable for building nanoscale platforms or devices. A wealth of evidence now demonstrates the potential of DNA nanotechnology in nanosensing applications. DNA nanostructures can interact with proteins, indigenous DNA and RNA, gold nanoparticles, quantum dots, DNAzymes, aptamers, and ions, among other molecules, and dynamically reconfigure to give out a reportable signal. DNA-based nanosystems also exemplify signal transduction from organic to inorganic complexes, signal amplification, and in vitro and in vivo biocompatibility. Last, but not least, synthetic DNA is amenable to storage under dried or hydrated conditions, a property that has expanded the diverse scenarios in which it can be transported and used. To that end, they are poised to form the basis of smarter diagnostic and biosensing tools that are modular, rapid, and possess several properties desired for advancing biomedicine.

For this Special Issue, we invite you to submit original research and review articles with a focus on the applications of DNA nanotechnology in biosensing and bioimaging. We welcome research that leverages the versatility of DNA nanotechnology as stand-alone nanosensors or as components that are coupled with physical phenomena such as resonance energy transfer, biological machines such as CRISPR-Cas, or sophisticated engineering in the form of advanced microscopy for biosensing/imaging purposes. DNA nanotechnology also encompasses non-canonical nucleic acids such as peptide nucleic acid and locked nucleic acid, which are emerging as robust alternatives to overcome degradation, and hence are of great interest. Review articles should describe the state of research in the area, the challenges that need to be addressed, and a perspective on the future of the field. We look forward to your contributions.

Dr. Divita Mathur
Dr. Igor Medintz
Guest Editors

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Keywords

  • Nanomaterials
  • DNA origami
  • Microscopy
  • FRET
  • Fluorescence
  • Imaging
  • Spectroscopy
  • CRISPR
  • Nanoparticles
  • Signal amplification

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Published Papers (1 paper)

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Research

11 pages, 2470 KiB  
Article
Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA
by Jhongryul Yoo, Hongin Jeong, Seo Kyung Park, Sungho Park and Je Seung Lee
Biosensors 2021, 11(7), 212; https://doi.org/10.3390/bios11070212 - 29 Jun 2021
Cited by 13 | Viewed by 3513
Abstract
Bioelectrodes mediated by metal oxide nanoparticles have facilitated the development of new sensors in medical diagnosis. High-purity TiO2 nanoparticles (NPs) were synthesized through thermal plasma and deposited directly on an interdigitated electrode. The surface of the TiO2-deposited electrode was activated [...] Read more.
Bioelectrodes mediated by metal oxide nanoparticles have facilitated the development of new sensors in medical diagnosis. High-purity TiO2 nanoparticles (NPs) were synthesized through thermal plasma and deposited directly on an interdigitated electrode. The surface of the TiO2-deposited electrode was activated with (3-aminopropyl) triethoxysilane (APTES) followed by fixing the single-stranded probe deoxyribonucleic acid (DNA) to fabricate the DNA biosensor. The structural properties of the deposited TiO2 nanoparticles were analyzed using a transmission electron microscope (TEM), X-ray diffraction (XRD), and a dynamic light scattering (DLS) system. The chemical composition and structural properties of the TiO2 nanoparticle layer and the fixed layer were analyzed by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). E. coli O157:H7, a well-known pernicious pathogenic bacterial species, was detected as a target DNA of the prepared DNA biosensor, and the characteristics of DNA detection were determined by the current change using a picoammeter. The degree of binding between the probe DNA and the target DNA was converted into an electrical signal using the picoammeter method to quantitatively analyze the concentration of the target DNA. With the specificity experiment, it was confirmed that the biosensor was able to discriminate between nucleotides with mismatched, non-complementary, or complementary sequences. Full article
(This article belongs to the Special Issue DNA Nanotechnology for Biosensing and Bioimaging)
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