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Biosensors
Special Issues
Electrokinetics-Assisted Biosensing
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Electrokinetics-Assisted Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 6109

Special Issue Editors

Prof. Dr. Yongjun Lai

E-Mail Website
Guest Editor
Department of Mechanical and Materials Engineering, Queen’s University, Kingston, ON K7L 3N6, Canada
Interests: MEMS; BioMEMS; sensors; instrumentation
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Ye Ai

E-Mail Website
Guest Editor
Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore
Interests: micro/nanofluidics technologies; bioMEMS; Lab-on-a-chip; acoustofluidics; electrokinetics; single cell analysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

I am pleased to announce a new Special Issue in Biosensors called “Electrokinetics-Assisted Biosensing”. Rapid detection of pathogens at low concentration in real time is still a challenging task. Various sensors can detect a single pathogen. However, at low concentration, pathogens take time to reach the sensing surface, which makes it hard to do real time detection. Electrokinetics including electroosmosis, electrophoresis and dielectrophoresis, and electrothermal phenomena have been widely explored in particle manipulations. Such particle manipulation techniques can be useful to accelerate the process of the pathogen landing onto the sensing surface, and hence, integration of such techniques with sensor will improve the sensors capability of real time detection. In this Special Issue, we invite researchers to contribute manuscripts that explore the integration of electrokinetics with sensors for rapid pathogen detection.

Dr. Yongjun Lai
Prof. Dr. Ye Ai
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrokinetics
  • electroosmosis
  • electrophoresis
  • dielectrophoresis
  • electrothermal
  • biosensing

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Published Papers (2 papers)

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Research

13 pages, 2952 KiB  
Article
3D Concentric Electrodes-Based Alternating Current Electrohydrodynamics: Design, Simulation, Fabrication, and Potential Applications for Bioassays
by Raphaela K. S. Silva, Sakandar Rauf, Ming Dong, Liang Chen, Hakan Bagci and Khaled N. Salama
Biosensors 2022, 12(4), 215; https://doi.org/10.3390/bios12040215 - 6 Apr 2022
Cited by 3 | Viewed by 2150
Abstract
Two-dimensional concentric asymmetric microelectrodes play a crucial role in developing sensitive and specific biological assays using fluid micromixing generated by alternating current electrohydrodynamics (ac-EHD). This paper reports the design, simulation, fabrication, and characterization of fluid motion generated by 3D concentric microelectrodes for the [...] Read more.
Two-dimensional concentric asymmetric microelectrodes play a crucial role in developing sensitive and specific biological assays using fluid micromixing generated by alternating current electrohydrodynamics (ac-EHD). This paper reports the design, simulation, fabrication, and characterization of fluid motion generated by 3D concentric microelectrodes for the first time. Electric field simulations are used to compare electric field distribution at the electrodes and to analyze its effects on microfluidic micromixing in 2D and 3D electrodes. Three-dimensional devices show higher electric field peak values, resulting in better fluid micromixing than 2D devices. As a proof of concept, we design a simple biological assay comprising specific attachment of streptavidin beads onto the biotin-modified electrodes (2D and 3D), which shows ~40% higher efficiency of capturing specific beads in the case of 3D ac-EHD device compared to the 2D device. Our results show a significant contribution toward developing 3D ac-EHD devices that can be used to create more efficient biological assays in the future. Full article
(This article belongs to the Special Issue Electrokinetics-Assisted Biosensing)
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Graphical abstract

12 pages, 2004 KiB  
Article
A V-Shaped Microcantilever Sensor Based on a Gap Method for Real-Time Detection of E. coli Bacteria
by Jino Fathy and Yongjun Lai
Biosensors 2022, 12(4), 194; https://doi.org/10.3390/bios12040194 - 25 Mar 2022
Cited by 4 | Viewed by 3260
Abstract
This paper presents a dynamic-mode microcantilever sensor based on a gap method. The sensor has a V-shaped microcantilever and a fixed structure at a distance of 2 µm from its free end. The microcantilever is excited by applying an ac electric potential (3 [...] Read more.
This paper presents a dynamic-mode microcantilever sensor based on a gap method. The sensor has a V-shaped microcantilever and a fixed structure at a distance of 2 µm from its free end. The microcantilever is excited by applying an ac electric potential (3 Vp) to its piezoelectric pads and vibrates at its fundamental resonant frequency. An independent ac electric potential (200 kHz, 15 Vpp) is applied to the fixed structure. This creates a non-uniform electric field with its maxima at the gap and exerts a dielectrophoresis (DEP) force. The DEP force attracts and adsorbs the E. coli bacteria to the cantilever edge at the gap. The binding of the bacteria to the cantilever creates a shift in the resonant frequency of the microcantilever sensor, which is detected by a laser vibrometer. The real-time detection of E. coli bacteria samples, diluted in distilled water, was performed for concentrations of 105–103 cells/mL and the real-time frequency shifts were −2264.3 to −755 Hz in 4 min, respectively. The tests were expanded to study the effect of the electric potential amplitude (10, 12, 15 Vpp) and higher frequency shifts were observed for higher amplitudes. Full article
(This article belongs to the Special Issue Electrokinetics-Assisted Biosensing)
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