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Biosensors and Diagnostic Platforms in Support of Global Health, Antimicrobial...
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Biosensors and Diagnostic Platforms in Support of Global Health, Antimicrobial Stewardship, Food Sustainability, and Water-Environmental Quality

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 13700

Special Issue Editor

Prof. Dr. Evangelyn C. Alocilja

E-Mail Website
Guest Editor
Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, Farrall Agricultural Engineering Hall, 524 S. Shaw Lane, Room 115, East Lansing, MI 48824-1323, USA
Interests: nanoparticle-based biosensors; PCR-less genomic detection; genome-phenome approaches to antimicrobial resistance (AMR) detection; beta-lactam (carbapenem) resistance
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue of Biosensors invites papers on novel approaches in biosensors and biosensing platforms that address unmet needs in the quick detection and diagnosis of diseases and biomarkers, particularly those with global societal impacts on human health, animal health, animal production, plant protection, water quality, environmental sustainability, food safety, biosecurity, and antimicrobial stewardship. The COVID-19 pandemic has also highlighted the need for the rapid detection of biomarkers and indicators in asymptomatic cases. A major focus of this edition is addressing the accessibility of these biosensing technologies in terms of affordability, simplicity, and resource-limited operability and how they could be incorporated in the arsenal of prevention strategies against future pandemics. Papers and abstracts presented at the “2021 Innovation Forum: Bridging Technologies and Market Needs” are welcome to this Special Edition.

Prof. Dr. Evangelyn Alocilja
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Published Papers (3 papers)

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Research

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19 pages, 1995 KiB  
Article
SMART-LAMP: A Smartphone-Operated Handheld Device for Real-Time Colorimetric Point-of-Care Diagnosis of Infectious Diseases via Loop-Mediated Isothermal Amplification
by Juan García-Bernalt Diego, Pedro Fernández-Soto, Sergio Márquez-Sánchez, Daniel Santos Santos, Begoña Febrer-Sendra, Beatriz Crego-Vicente, Juan Luis Muñoz-Bellido, Moncef Belhassen-García, Juan M. Corchado Rodríguez and Antonio Muro
Biosensors 2022, 12(6), 424; https://doi.org/10.3390/bios12060424 - 16 Jun 2022
Cited by 26 | Viewed by 5291
Abstract
Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed and robustness, require extensive infrastructure, and are neither affordable nor user-friendly. Thus, they have not been extensively applied in point-of-care diagnostics, particularly in low-resource settings. In this [...] Read more.
Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed and robustness, require extensive infrastructure, and are neither affordable nor user-friendly. Thus, they have not been extensively applied in point-of-care diagnostics, particularly in low-resource settings. In this work, we have combined the loop-mediated isothermal amplification (LAMP) technology with a handheld portable device (SMART-LAMP) developed to perform real-time isothermal nucleic acid amplification reactions, based on simple colorimetric measurements, all of which are Bluetooth-controlled by a dedicated smartphone app. We have validated its diagnostic utility regarding different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19, and analyzed clinical samples from suspected COVID-19 patients. Finally, we have proved that the combination of long-term stabilized LAMP master mixes, stored and transported at room temperature with our developed SMART-LAMP device, provides an improvement towards true point-of-care diagnosis of infectious diseases in settings with limited infrastructure. Our proposal could be easily adapted to the diagnosis of other infectious diseases. Full article
(This article belongs to the Special Issue Biosensors and Diagnostic Platforms in Support of Global Health, Antimicrobial Stewardship, Food Sustainability, and Water-Environmental Quality)
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10 pages, 2092 KiB  
Article
Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
by Zhihang Xiong, Hongjian Wang, Jiajin Li, Ran Liao, Haoji Mai, Caizhong Guan, Zhiming Guo, Shangpan Yang, Yan Chen, Biwang Liu, Tong Liu, Hongyi Li, Wenzheng Ding, Yaguang Zeng and Hui Ma
Biosensors 2021, 11(11), 416; https://doi.org/10.3390/bios11110416 - 25 Oct 2021
Cited by 8 | Viewed by 2302
Abstract
Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and [...] Read more.
Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of Synechococcus. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring. Full article
(This article belongs to the Special Issue Biosensors and Diagnostic Platforms in Support of Global Health, Antimicrobial Stewardship, Food Sustainability, and Water-Environmental Quality)
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Review

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15 pages, 1280 KiB  
Review
Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review
by Emma Dester and Evangelyn Alocilja
Biosensors 2022, 12(2), 112; https://doi.org/10.3390/bios12020112 - 11 Feb 2022
Cited by 23 | Viewed by 4593
Abstract
Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the [...] Read more.
Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the use of glycan-coated magnetic nanoparticles (MNPs) is often omitted. This review seeks to analyze the potential of this technique as a rapid and cost-effective solution for concentration of bacteria directly from foods. The primary focus is the mechanism of glycan-coated MNP binding, as well as its current applications in concentration of foodborne pathogens. First, a background on the synthesis, properties, and applications of MNPs is provided. Second, synthesis of glycan-coated particles and their theorized mechanism for bacterial adhesion is described. Existing research into extraction of bacteria directly from food matrices is also analyzed. Finally, glycan-coated MNPs are compared to the magnetic separation technique of immunomagnetic separation (IMS) in terms of cost, time, and other factors. At its current state, glycan-coated MNPs require more research to fully identify the mechanism, potential for optimization, and extraction capabilities directly in food matrices. However, current research indicates glycan-coated MNPs are an incredibly cost-effective method for rapid food pathogen extraction and concentration. Full article
(This article belongs to the Special Issue Biosensors and Diagnostic Platforms in Support of Global Health, Antimicrobial Stewardship, Food Sustainability, and Water-Environmental Quality)
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