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Biosensors
Special Issues
Fluorescent Biosensors: New Methods, Devices and Important Applications
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Fluorescent Biosensors: New Methods, Devices and Important Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 3751

Special Issue Editors

Prof. Dr. Qi Zhang

E-Mail Website
Guest Editor
National Reference Lab for Agricultural Testing (Biotoxin), Key Lab of Detection for Mycotoxin, Ministry of Agriculture and Rural Affairs PRC, Lab of Quality & Safety Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture and Rural Affairs PRC, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Interests: fluorescence immunosensor; functional biomaterials and nanomaterials for capture; analysis; diagnosis; warning; prevention of and reduction in toxins
Special Issues, Collections and Topics in MDPI journals
Dr. Jean Jacques Toulme

E-Mail Website
Guest Editor
Novaptech, 33600 Pessac, France
Interests: aptamer; synthetic oligonucleotide; aptasensor; fluorescence detection; light-up aptamer; molecular beacon; SPR
Dr. Masanobu Iwanaga

E-Mail Website
Guest Editor
National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan
Interests: fluorescence enhancement; nanostructure-based biosensors; metasurfaces; metamaterials; nanophotonics; plasmonics

Special Issue Information

Dear Colleagues,

Fluorescence is a type of important signal in biosensors, which is usually highly sensitive. It has been paid significant attention in biotracing (e.g., intracellular protein interaction or location), diagnosis (e.g., pathogen diagnosis for human, livestock, and crops), analytical chemistry (e.g., detection contaminant/chemical in food or environmental samples), and other fields. Fluorescent materials include organic compounds, proteins, inorganic fluorescent materials, etc. Resonance fluorescence, polarization fluorescence, time-resolved fluorescence, and other methods have emerged. This Special Issue focuses on fluorescence biosensing, including (but not limited to) new methods, new devices, important new applications, etc. We invite researchers to share their work on fluorescent biosensors, and original research articles and comprehensive reviews will be considered for publication.

Prof. Dr. Qi Zhang
Dr. Jean Jacques Toulme
Dr. Masanobu Iwanaga
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescence
  • biosensor
  • method
  • device
  • application
  • food
  • agriculture
  • environment
  • pathogen
  • contaminant

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Published Papers (3 papers)

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Research

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10 pages, 3726 KiB  
Article
Needle Tip Tracking through Photoluminescence for Minimally Invasive Surgery
by Meenakshi Narayan and Mithun Bhowmick
Biosensors 2024, 14(10), 470; https://doi.org/10.3390/bios14100470 - 30 Sep 2024
Viewed by 687
Abstract
Minimally invasive surgery continues to prioritize patient safety by improving imaging techniques and tumor detection methods. In this work, an all-optical alternative to the current image based techniques for in vitro minimally invasive procedures has been explored. The technique uses a highly fluorescent [...] Read more.
Minimally invasive surgery continues to prioritize patient safety by improving imaging techniques and tumor detection methods. In this work, an all-optical alternative to the current image based techniques for in vitro minimally invasive procedures has been explored. The technique uses a highly fluorescent marker for the surgical needle to be tracked inside simulated tissues. A series of markers were explored including inorganic (Perovskite and PbS) and organic (carbon dots) nanoparticles and organic dye (Rhodamine 6G) to identify layers of different stiffnesses within a tissue. Rhodamine 6G was chosen based on its high fluorescence signal to track 3D position of a surgical needle in a tissue. The needle was tracked inside homogeneous and inhomogeneous gelatin tissues successfully. This exploratory study of tissue characterization and needle tip tracking using fluorescent markers or photoluminescence technique show potential for real-time application of robot-assisted needle insertions during in vivo procedures. Full article
(This article belongs to the Special Issue Fluorescent Biosensors: New Methods, Devices and Important Applications)
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Review

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17 pages, 10881 KiB  
Review
Role of Peroxynitrite in the Pathogenesis of Parkinson’s Disease and Its Fluorescence Imaging-Based Detection
by Jiye Lv, Feiyu Chen, Changchan Zhang, Yubing Kang, Yan Yang and Chengwu Zhang
Biosensors 2024, 14(10), 506; https://doi.org/10.3390/bios14100506 - 17 Oct 2024
Viewed by 740
Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disorder, affecting the lives of millions of people worldwide. Although the mechanism underlying PD pathogenesis is largely undefined, increasing evidence indicates that oxidative and nitrosative stresses play a crucial role in PD occurrence and [...] Read more.
Parkinson’s disease (PD) is the second most common neurodegenerative disorder, affecting the lives of millions of people worldwide. Although the mechanism underlying PD pathogenesis is largely undefined, increasing evidence indicates that oxidative and nitrosative stresses play a crucial role in PD occurrence and development. Among them, the role of oxidative stress has been widely acknowledged, but there is relatively less attention given to nitrosative stress, which is mainly derived from peroxynitrite. In the present review, after briefly introducing the background of PD, we discuss the physiopathological function of peroxynitrite and especially highlight how overloaded peroxynitrite is involved in PD pathogenesis. Then, we summarize the currently reported fluorescence imaging-based peroxynitrite detection probes. Moreover, we specifically emphasize the probes that have been applied in PD research. Finally, we propose perspectives on how to develop a more applicable peroxynitrite probe and leverage it for PD theranostics. Conclusively, the present review broadens the knowledge on the pathological role of peroxynitrite in the context of PD and sheds light on how to develop and utilize fluorescence imaging-based strategies for peroxynitrite detection. Full article
(This article belongs to the Special Issue Fluorescent Biosensors: New Methods, Devices and Important Applications)
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31 pages, 3609 KiB  
Review
Fluorogenic RNA-Based Biosensors of Small Molecules: Current Developments, Uses, and Perspectives
by Janine Kehrli, Claire Husser and Michael Ryckelynck
Biosensors 2024, 14(8), 376; https://doi.org/10.3390/bios14080376 - 1 Aug 2024
Viewed by 1649
Abstract
Small molecules are highly relevant targets for detection and quantification. They are also used to diagnose and monitor the progression of disease and infectious processes and track the presence of contaminants. Fluorogenic RNA-based biosensors (FRBs) represent an appealing solution to the problem of [...] Read more.
Small molecules are highly relevant targets for detection and quantification. They are also used to diagnose and monitor the progression of disease and infectious processes and track the presence of contaminants. Fluorogenic RNA-based biosensors (FRBs) represent an appealing solution to the problem of detecting these targets. They combine the portability of molecular systems with the sensitivity and multiplexing capacity of fluorescence, as well as the exquisite ligand selectivity of RNA aptamers. In this review, we first present the different sensing and reporting aptamer modules currently available to design an FRB, together with the main methodologies used to discover modules with new specificities. We next introduce and discuss how both modules can be functionally connected prior to exploring the main applications for which FRB have been used. Finally, we conclude by discussing how using alternative nucleotide chemistries may improve FRB properties and further widen their application scope. Full article
(This article belongs to the Special Issue Fluorescent Biosensors: New Methods, Devices and Important Applications)
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