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Biosensors
Special Issues
Advances in Immunoassay Biosensing Platform for Point-of-Care Diagnostics
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Advances in Immunoassay Biosensing Platform for Point-of-Care Diagnostics

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 3056

Special Issue Editors

Dr. Jasmine Devadhasan

E-Mail Website
Guest Editor
College of Medicine-Phoenix, University of Arizona, Phoenix, AZ 85004, USA
Interests: point-of-care diagnostics; immunosensors; colorimet-ric assay; surface modification; electrochemical; lateral flow device; vertical flow device; microarray technology; biothreat agents; environmental monitoring
Prof. Dr. Sergei A. Eremin

E-Mail Website
Guest Editor
Department of Chemical Enzymology, Faculty of Chemistry, Moscow State University, Moscow 119991, Russia
Interests: immunosensors; fluorescence; antibody; immunoanalytical techniques for food safety and environmental control
Prof. Dr. Dapeng Peng

E-Mail Website
Guest Editor
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, China
Interests: rapid detection technology; veterinary drug residues; animal derived food; food safety
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Séamus Higson

E-Mail Website
Guest Editor
Cranfield Health, Cranfield University, Cranfield MK43 0AL, UK
Interests: electrochemical biosensors; biosensors for healthcare; biosensors for environmental monitoring; DNA sensors; enzymatic sensors; affinity based biosensors; antibody based biosensors; micro-electrodes and micro-electrode arrays
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Immunoassays are widely applied for the detection of diseases, bacteria, viruses, drugs, allergens, toxins in food, and other biomolecules. In the last several years, the clinical demand for rapid immunodiagnostics has been demonstrated in immediate proximity to minimizing the spread of infection at an early stage. In that respect, point-of-care (POC) diagnostics have been proven to be substantial portable analytical tools, especially lateral flow, vertical flow, wearable, and optical immunoassay platforms. However, the demand for highly sensitive, and accurate point-of-care platforms in clinical and low-resource settings is ever increasing. Therefore, this Special Issue, "Advances in Immunoassay Biosensing Platform for Point-of-Care Diagnostics", focuses on the recent advances in immunoassay platforms for the detection of antigens, antibodies, proteins, small molecules, DNA, and RNA with the integration of advanced or innovative detection technologies, sample processing, and labeling molecules to apply in point-of-care diagnostics. We invite submissions of research that help to advance immunoassay biosensing platforms for point-of-care diagnostics and its application for rapid diagnosis in low-resource settings.

Dr. Jasmine Devadhasan
Prof. Dr. Sergei A. Eremin
Prof. Dr. Dapeng Peng
Prof. Dr. Séamus Higson
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • immunosensor
  • point of need
  • microarray
  • rapid detection
  • technology integration
  • sample processing
  • assay methodology
  • biomarkers
  • immunochromatographic tests
  • instrumentation

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Published Papers (2 papers)

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Research

15 pages, 4571 KiB  
Article
Lateral Flow Assay for Preeclampsia Screening Using DNA Hairpins and Surface-Enhanced Raman-Active Nanoprobes Targeting hsa-miR-17-5p
by Ka Wai Ng, Siddhant Jaitpal, Ngoc Nhu Vu, Angela Michelle T. San Juan, Sayantan Tripathy, Rohit Sai Kodam, Abhishek Bastiray, Jae-Hyun Cho, Mahua Choudhury, Gerard L. Coté and Samuel Mabbott
Biosensors 2024, 14(11), 535; https://doi.org/10.3390/bios14110535 - 5 Nov 2024
Viewed by 659
Abstract
Preeclampsia (PE) is a serious complication that poses risks to both mothers and their children. This condition is typically asymptomatic until the second or even third trimester, which can lead to poor outcomes and can be costly. Detection is particularly challenging in low- [...] Read more.
Preeclampsia (PE) is a serious complication that poses risks to both mothers and their children. This condition is typically asymptomatic until the second or even third trimester, which can lead to poor outcomes and can be costly. Detection is particularly challenging in low- and middle-income countries, where a lack of centralized testing facilities coincides with high rates of PE-related maternal mortality. Variations in the levels of hsa-miR-17-5p have been identified as constituting a potential early indicator for distinguishing between individuals with PE and those without PE during the first trimester. Thus, developing a screening test to measure hsa-miR-17-5p levels would not only facilitate rapid detection in the early stages of pregnancy but also help democratize testing globally. Here, we present a proof-of-principle lateral-flow assay (LFA) designed to measure hsa-miR-17-5p levels using DNA-hairpin recognition elements for enhanced specificity and nanoprobes for sensitive surface-enhanced resonance Raman scattering (SERS) signal transduction. The theoretical limit of detection for hsa-miR-17-5p was 3.84 × 10−4 pg/µL using SERS. Full article
(This article belongs to the Special Issue Advances in Immunoassay Biosensing Platform for Point-of-Care Diagnostics)
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14 pages, 4682 KiB  
Article
Highly Sensitive Magnetic-Nanoparticle-Based Immunochromatography Assay for Rapid Detection of Amantadine in Chicken and Eggs
by Huaming Li, Yanrong Lu, Linwei Zhang, Liangni Qin, Hao Wen, Xiaohui Fan and Dapeng Peng
Biosensors 2024, 14(1), 23; https://doi.org/10.3390/bios14010023 - 30 Dec 2023
Cited by 2 | Viewed by 1821
Abstract
Amantadine (AMD) is an antiviral drug that is prohibited for use in livestock and poultry. In this study, carboxyl-modified magnetic nanoparticles (MNPs) were synthesized using the solvothermal method in one step with harmless and inexpensive regents, and they were used to label monoclonal [...] Read more.
Amantadine (AMD) is an antiviral drug that is prohibited for use in livestock and poultry. In this study, carboxyl-modified magnetic nanoparticles (MNPs) were synthesized using the solvothermal method in one step with harmless and inexpensive regents, and they were used to label monoclonal antibodies (mAbs) of AMD in microwells with electrostatic adsorption. Then, a magnetic immunochromatography assay (MICA) method was successfully established. Under optimal conditions, the MICA showed a good performance, with a linear range of 0.2~10.0 µg/L. The limit of detection (LOD) was 0.068 µg/L with the instrument, and the visual LOD (vLOD) was 0.5 µg/L. There was no cross-reaction with rimantadine and ribavirin. The vLOD in real samples was 1.0 µg/kg. The developed MICA has the advantages of convenience, speed, and sensitivity, which make it suitable for the on-site rapid detection of AMD residues in chicken tissues and eggs. Full article
(This article belongs to the Special Issue Advances in Immunoassay Biosensing Platform for Point-of-Care Diagnostics)
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