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Foods
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DNA-Based Authentication of Fish and Fish Products
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DNA-Based Authentication of Fish and Fish Products

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Engineering and Technology".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 9757

Special Issue Editors

Prof. Dr. Fausto Tinti

E-Mail Website
Guest Editor
Department of Biological, Geological and Environmental Sciences, Alma Mater Studiorum University of Bologna, 48121 Ravenna, Italy
Interests: evolutionary patterns and processes in marine animals; spatio-temporal changes of genetic and genomic variation in epipelagic marine fish and of correlations with environmental/ecological drivers, including human ones; fish, fish populations and seafood product traceability
Prof. Dr. Alessia Cariani

E-Mail Website
Guest Editor
Department of Biological, Geological and Environmental Sciences, Alma Mater Studiorum University of Bologna, 48121 Ravenna, Italy
Interests: identification and description of the evolutionary and environmental drivers of biodiversity in marine taxa throughout the development and application of innovative genetic and genomic tools; technological application of genomic tools to the sustainable management of fishery resources; disentangling neutral and adaptive variation in relevant commercial fishery and aquacultured marine species and exploit scientific results in a traceability and management context

Special Issue Information

Dear Colleagues,

Evidence from DNA-based Technologies and Analyses (i.e., DNA-Tools) is increasingly being used to contrast food infringements and malpractices. To date, DNA-Tools application to control and enforce rules and legal activities along with the seafood supply-chain results still sporadic, though EU’s the Farm to Fork Strategy is committing the improvement and digitalization of traceability of seafood products to make them traceable from point-of-catch to point-of-sale, as a priority in order to combat Illegal, Unreported and Unregulated fishing, achieve healthy fisheries and to make seafood production ecologically-sustainable and deliver benefits for consumers and communities. DNA-Tools are also capable to support fishery certification programs and ecolabels have emerged to promote fisheries and seafood sustainability, giving added-value to eco-certified seafood products. Modern DNA-Tools can address a broad range of authentication issues in the seafood supply-chain, from species mislabelling of fish and fish products to determining the origin of catches and farmed livestock.

This Special Issue focuses on authentication evidence obtained by DNA-Tools along with primary productions from fisheries and aquaculture and the seafood product supply-chain. Cutting-edge contributions on the DNA-based authentication of fishery/aquaculture resources and products which are potentially or effectively target for certification programmes and ecolabeling are considered as frontier contributions toward healthy fisheries and ecologically-sustainable seafood supply-chains.

Prof. Dr. Fausto Tinti
Prof. Dr. Alessia Cariani
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fish
  • seafood
  • DNA-based technologies
  • genetic markers
  • genomic markers
  • traceability
  • fish food products
  • ecolabel
  • fishery resources
  • aquacultured livestock

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Published Papers (3 papers)

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Research

10 pages, 2979 KiB  
Communication
Multiplex PCR Assay for Simultaneous Identification of Five Types of Tuna (Katsuwonus pelamis, Thunnus alalonga, T. albacares, T. obesus and T. thynnus)
by Ga-Young Lee, Seung-Man Suh, Yu-Min Lee and Hae-Yeong Kim
Foods 2022, 11(3), 280; https://doi.org/10.3390/foods11030280 - 20 Jan 2022
Cited by 16 | Viewed by 3575
Abstract
There is a need to identify the species of similar types of fish, especially those that are commercially sold. Particularly, the price of tuna varies depending on its type, which is difficult to determine as they are sold in cut or processed forms. [...] Read more.
There is a need to identify the species of similar types of fish, especially those that are commercially sold. Particularly, the price of tuna varies depending on its type, which is difficult to determine as they are sold in cut or processed forms. This study developed a multiplex polymerase chain reaction (PCR) assay to identify the five most common tuna species: bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas. Newly designed species-specific primer sets for these five tuna species were created. Subsequently, the amplicon sizes obtained were 270, 238, 200, 178, and 127 base pairs for bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas, respectively. Each primer’s specificity was further tested using 15 other fish species, and no cross-reactivity was observed. To identify multiple targets in a single reaction, multiplex PCR was optimized to increase its resolution and accuracy. The detection levels of the multiplex PCR assay were confirmed to be 1 pg for all the five tunas. Additionally, it was successfully applied to 32 types of commercial tuna products. Therefore, this multiplex PCR assay could be an efficient identification method for various tuna species. Full article
(This article belongs to the Special Issue DNA-Based Authentication of Fish and Fish Products)
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Graphical abstract

11 pages, 18935 KiB  
Communication
A Multiplex PCR Assay Combined with Capillary Electrophoresis for the Simultaneous Identification of Atlantic Cod, Pacific Cod, Blue Whiting, Haddock, and Alaska Pollock
by Yu-Min Lee, Shinyoung Lee and Hae-Yeong Kim
Foods 2021, 10(11), 2631; https://doi.org/10.3390/foods10112631 - 29 Oct 2021
Cited by 13 | Viewed by 2174
Abstract
With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed [...] Read more.
With an increased consumption of seafood products, food fraud with fish resources has been continuously reported. In particular, codfish has been exploited worldwide as a processed product in fresh, frozen, smoked, canned, or ready-to-eat dish forms. However, it is challenging to identify processed fish products after processing because of their similar morphological characteristics. Substitution and mislabeling of codfish among different species are also happening deliberately or unintentionally. Thus, it is necessary to distinguish cod species to prevent fish adulteration and food fraud. In this study, we developed a multiplex PCR for simultaneously identifying five cod species within Gadidae using capillary electrophoresis. Then, their species-specific primer sets were designed by targeting the mitochondrial cytochrome b gene. Subsequently, the amplicon sizes obtained were 237 bp, 204 bp, 164 bp, 138 bp, and 98 bp for Atlantic cod, Pacific cod, blue whiting, haddock, and Alaska pollock, respectively. The specificity of each primer was further tested using 19 fish species, and no cross-reactivity was observed. The limit of detection of this multiplex PCR assay was 1 pg. The developed multiplex PCR assay can be applied to 40 commercial food products successfully. This detection method will be efficient for managing seafood authentication by simultaneously analyzing multiple cod species. Full article
(This article belongs to the Special Issue DNA-Based Authentication of Fish and Fish Products)
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14 pages, 1884 KiB  
Article
A New Rapid Method for the Authentication of Common Octopus (Octopus vulgaris) in Seafood Products Using Recombinase Polymerase Amplification (RPA) and Lateral Flow Assay (LFA)
by Amaya Velasco, Graciela Ramilo-Fernández, Françoise Denis, Luís Oliveira, Peter Shum, Helena Silva and Carmen G. Sotelo
Foods 2021, 10(8), 1825; https://doi.org/10.3390/foods10081825 - 6 Aug 2021
Cited by 17 | Viewed by 2915
Abstract
The common octopus (Octopus vulgaris) is a highly valued cephalopod species which is marketed with different grades of processing, such as frozen, cooked or even canned, and is likely to be mislabeled. Some molecular methods have been developed for the authentication [...] Read more.
The common octopus (Octopus vulgaris) is a highly valued cephalopod species which is marketed with different grades of processing, such as frozen, cooked or even canned, and is likely to be mislabeled. Some molecular methods have been developed for the authentication of these products, but they are either labor-intensive and/or require specialized equipment and personnel. This work describes a newly designed rapid, sensitive and easy-to-use method for the detection of Octopus vulgaris in food products, based on Recombinase Polymerase Amplification (RPA) and a detection using a Lateral Flow assay (LFA). After studying several gene markers, a system of primers and nfo-probe was designed in the COI (Cytochrome Oxidase I) region and was successfully tested in 32 reference samples (covering 14 species) and 32 commercial products, after optimization. The method was also validated in a ring trial with eight European laboratories and represents a useful tool for food authenticity control at all levels of the value chain. Full article
(This article belongs to the Special Issue DNA-Based Authentication of Fish and Fish Products)
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